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CAB6P和CAB11E 2种脱毒甜樱桃砧木嫩梢茎尖为试材,成功地进行了离体快繁研究。结果表明:外植体在改良的DKW培养基上进行诱导培养和继代增殖培养最好,在1/2MS培养基(铁盐为DKW)生根较好;同时特定的液体培养基可以显著地将增殖培养的不定稍拉长。温室中的温度、湿度控制和病害防治是试管苗移栽成活率的一个关键环节。  相似文献   
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《Journal of plant nutrition》2013,36(10-11):2197-2210
Abstract

The unicellular green alga Dunaliella was previously proposed as a model photosynthetic organism for adaptation to iron deficiency. The purpose of this study was to find out how iron limitation affects the structure and composition of the photosynthetic system of Dunaliella salina. Iron deprivation did not retard proliferation of D. salina cells, but was associated with a decrease in cell volume and chlorophyll content, and with a four‐fold reduction in iron content and a two‐fold increase in Cu content. Electron microscopic analysis revealed shrinkage of the chloroplast and decrease in stacked thylakoid membranes. Measurements of chlorophyll fluorescence induction in the presence of DCMU and of 77 K chlorophyll fluorescence emission spectra indicated gross changes in the photosynthetic efficiency of reaction centers and in the organization of their associated light harvesting antenna. Differential analysis of protein composition led to the identification of a major thylakoid membrane protein (Tidi), that was specifically induced under iron deprivation. Partial sequencing suggests that Tidi is a novel type of a chlorophyll a/b binding protein. These results clearly show that iron limitation is associated with extensive reorganization of the photosynthetic system in Dunaliella.  相似文献   
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ABSTRACT

Identifying the best source of information to satisfy the needs of local clientele has always been the challenge facing collection development and instruction librarians. In order to provide users with the best possible access tool and source for comprehensive information, it is important that librarians be aware of the most productive sources of information in a field. This paper identifies where the bulk of agricultural economics research is published and indexed. It also ascertains whether Google Scholar is as productive in covering this information as CAB Abstracts and EconLit. The cited reference counts for the top 50 cited sources in Web of Knowledge and Google Scholar are also compared. The scatter of the journal literature in this field, based on the Library of Congress Subject Headings, is also provided. The study identified a broad range of article scattering in areas where agricultural economics and agribusiness materials are published. On the cited reference count, Google Scholar was as productive as the Web of Knowledge. Google Scholar is a free source of very useful information for cited references and other subject searches in the area of agricultural economics and could be used to complement traditional databases.  相似文献   
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对甜菜夜蛾等害虫具有高毒力的苏云金杆菌CAB109菌株进行生物学特性调查结果表明,CAB109菌株的伴孢晶体具有典型的菱形结构;其原毒素蛋白的分子量为133kDa,经胰蛋白酶处理后水解为66kDa的活性毒素蛋白.DNA凝胶电泳显示:CAB19菌株的质粒DNA与同样H,血清型的已知HD-133菌株不同.CAB109菌株对葱田甜菜夜蛾的平均防效达75.3%,高于常用化学农药和微生物农药的防效;平均挽回损失率也达26.0%表现出优良的开发潜力.  相似文献   
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This study examined the 130 current full-text titles in the BioOne.1 and BioOne.2 collections to determine whether they are peer-reviewed, contain book reviews, and are supplied by document delivery vendors. We identified indexing coverage in 11 major databases in agriculture and life sciences: Aquatic Sciences and Fisheries Abstracts (ASFA), AGRICOLA, Biological Abstracts, BIOSIS Previews, CAB Abstracts, Current Contents, Environmental Sciences and Pollution Abstracts, GeoRef, Scopus, ISI Web of Science, and Zoological Record. We concluded that the majority of the titles in BioOne collections are refereed and scholarly, indexed in several of the major indexing and abstracting journals in the field of agriculture and life sciences, and available via several major document delivery vendors. We find both BioOne collections to be valuable, useful, cost-effective journal aggregations.  相似文献   
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Search检索模式中检索词的选择技巧   总被引:1,自引:0,他引:1  
作者通过对世界第一大农业文献数据库——CAB ABSTRACTS光盘数据库的主要检索模式(Search)中检索词的选择技巧问题进行了研究,提出了四种选择方法,并且对几种选择方法的特点、适用范围等进行了比较研究,为更好地利用数据库进行信息检索提供了有效的方法。  相似文献   
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本文采用文献计量学的方法 ,对世界著名的农业文献检索系统CAB光盘数据库收录山东农业大学 1990~ 2 0 0 0年间发表的文献数量、学科、作者、原文语种、英文摘要率和期刊分布等状况进行了统计分析 ,揭示了CAB收录山东农业大学论文的现状与特点 ,并就增加文献收录量提出了几点建议  相似文献   
8.
以一编码蛋白与拟南芥的CAB1高度相似的EST序列作为探针,通过同源搜索从橡胶树的转录组数据中电子克隆到一条长1 415 bp的cDNA,该序列包含一798 bp的ORF及47 bp的5′UTR和570 bp的3′UTR,命名为HbCAB1;在此基础上,采用PCR技术成功扩增了包括该ORF在内的915 bp的cDNA序列。序列分析表明,该基因预测编码265个氨基酸,理论分子量为28.15 ku,等电点为5.25;蛋白含有1个保守的捕光叶绿素a/b结合蛋白结构域,3个跨膜螺旋和1个C端螺旋,1个三聚化基序,  相似文献   
9.
ABSTRACT

Selecting a service involves evaluating many criteria, including price, ease of use, user interface, value-added information, and title coverage. This study examines four current awareness services, Current Contents: Agriculture, Biology and Environmental Science, CARL UnCover, ContentsFirst, and CAB ACCESS, for their coverage of serials in agriculture and related fields. The serial lists from the services were examined for overlapping and unique titles, subject coverage, and publication type. UnCover was found to include not only the greatest number of titles but also the highest percentage of unique titles. However, because a relatively low percentage of the unique titles in UnCover are academic/scholarly and foreign/multilingual, it is recommended that a second service be considered to complement UnCover.  相似文献   
10.
CAB收录南京农业大学科技论文的统计与分析   总被引:1,自引:1,他引:0  
采用文献计量学的方法,以1990~2003年CAB数据库为数据源,对农业高校被录用文献数量、南京农业大学科技论文的学科分布、作者的文献数量分布及期刊分布进行了统计分析,从一个侧面反映了南京农业大学科学研究状况。最后提出了增加我校文献被CAB收录的几点建议。  相似文献   
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