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胆囊收缩素主动免疫产蛋鸡的营养生理效应研究 总被引：1，自引：0，他引：1
用胆囊收缩素(CCK)主动免疫产蛋鸡，研究产蛋鸡血清和卵黄中CCK特异性抗体的产生规律及不同CCK免疫剂量对产蛋鸡生产性能和胰腺、胆囊发育及内分泌激素的影响。结果显示：用CCK-8主动免疫产蛋鸡，可以提高血清和卵黄CCK抗体水平，降低血液中CCK水平；CCK抗体的产生量在第1次加强免疫后即可接近高峰，第2、第3次加强免疫可维持较高的抗体水平。CCK主动免疫产蛋鸡后，对蛋鸡胰腺、胆囊发育、胰蛋白含量以及蛋鸡体内的生长激素、胰岛素和瘦素水平均没有显著影响，同时对蛋鸡采食量和料蛋比等生产性能也没有显著影响。用CCK主动免疫产蛋鸡，在不影响蛋鸡正常生理功能的同时，可以获得含特异性CCK抗体的卵黄。 相似文献
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本试验旨在探讨日粮中添加CC-Dp对马驹麦草采食量和血清中摄食调控激素的影响。选取20匹17月龄健康无病、体况和采食量相近的伊犁马驹,采用单因素试验设计,随机分为4组,每组5匹,对照组饲喂基础日粮,试验Ⅰ、Ⅱ、Ⅲ组分别在基础日粮中添加500、1000、1500 mg/kg CC-Dp。预试期7 d,正试期30 d。结果表明,与对照组相比,试验Ⅱ组马驹麦草采食量在试验第1~15天显著提高(P<0.05),血清中胆囊收缩素(CCK)和γ-氨基丁酸(GABA)含量在试验进行1~15 d分别显著降低和升高(P<0.05);与对照组相比,试验Ⅲ组马驹麦草采食量在试验全期内显著提高(P<0.05),血清中CCK含量在试验进行1~15 d极显著降低(P<0.01)、16~30 d显著降低(P<0.05),血清中GABA含量在试验全期均显著提高(P<0.05);各组间血清中神经肽Y(NPY)、5-羟色胺(5-HT)、瘦素(LEP)含量差异均不显著(P>0.05)。综上所述,日粮中添加1500 mg/kg CC-Dp能显著提高伊犁马驹麦草采食量,显著降低血清中胆囊收缩素含量,显著升高血清中γ-氨基丁酸含量,推测其作用机制可能是通过降低动物体内胆囊收缩素水平以调控动物的采食,且与γ-氨基丁酸的调控途径有关。 相似文献
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【目的】胃泌素(GAS)和胆囊收缩素(CCK)具有较多相似性,但两种激素与其受体(CCK-A和CCK-B)亲和力不同,研究利用生物信息学的方法,比对羊GAS和CCK及其受体氨基酸序列与其它动物序列的不同,为不同动物这两种多肽及其受体蛋白抗原设计和活性多肽筛选等提供参考。【方法】通过UniProt数据库检索各种动物GAS和CCK及其受体氨基酸序列进行比对,并进行全氨基酸序列进化树分析。【结果】除了鸡和鱼,其他动物GAS的C-端7个氨基酸完全相同;除了豚鼠外,CCK的C-端10个氨基酸序列完全相同;羊与牛的GAS-34及CCK-33氨基酸序列完全相同。羊与牛,大鼠与小鼠、狗与猫的CCK-A和CCK-B均分别在同一个分支上;从GAS和CCK及受体全氨基酸序列(包括信号肽和原肽)来看,禽类和鱼类序列同哺乳动物差距均较大;绵羊CCK-A与CCK-B氨基酸序列相似性仅为45.094%。【结论】GAS和CCK的C-端氨基酸序列在不同动物之间均具有高度的保守性,而N-端为变化区域。 相似文献
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斑节对虾性腺组织、细胞的原代培养条件优化 总被引：1，自引：0，他引：1
通过观察不同成分培养基及不同培养方法培养的斑节对虾性腺组织及细胞原代培养生长情况,分析细胞活力及生长相关基因表达情况,判断3种改良培养基的适用条件,为进一步细胞培养试验提供支持。试验结果表明,以L-15培养基为基础培养基,不同配方的培养效果差距较大,其中机械分离法可成功培养离体斑节对虾性腺组织,而酶解法与3号培养基结合效果较好;在培养过程中可见离体卵母细胞多呈悬浮生长,部分可见分裂相,离体精原细胞多呈贴壁生长,1号、2号培养基中的细胞可维持良好生长状态达一周以上;CCK测试结果表明,机械分离的性腺组织和酶解法分离的性腺细胞分别在1号、3号培养基中有更高的细胞活力,而2号培养基中的性腺组织及细胞在两种培养方法下的活力差别不大。进一步的荧光定量PCR检测了离体培养过程中MAPK信号通路相关基因的表达变化情况,结果表明,Ras、c-Mos、MEK1、ERK基因在卵母细胞中表达变化更显著,且c-Mos基因与离体细胞生长发育情况呈正相关。本试验系统性地筛选了适宜斑节对虾性腺组织、细胞的原代培养方法,为了解培养过程中的生理过程变化提供了相关参考数据。 相似文献
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Onaga T 《Veterinary research communications》2007,31(8):1021-1036
The effects of intravenous infusion of proglumide on regular ruminal contractions were examined in conscious sheep using doses
that inhibit pancreatic exocrine secretion. After a control period of 20 min, proglumide was infused intravenously for 40
min at a dose of 15, 30 or 60 μmol/kg per min and venous blood was collected. The intravenous infusion of proglumide significantly
increased the frequency of ruminal contractions at 15 μmol/kg per min without altering the amplitude, while it significantly
decreased the frequency and amplitude of ruminal contractions at 30 and 60 μmol/kg per min in a dose-dependent manner. Proglumide
did not increase contractile activity of the omasum, abomasum and duodenum or the plasma concentration of immunoreactive cholecystokinin
(CCK). Application of proglumide at 1–30 mmol/L inhibited bethanechol-induced contraction in both longitudinal and circular
muscle strips of the dorsal sac of the rumen. These results suggest that proglumide at a low dose acts indirectly on the rumen
as a CCK receptor antagonist to increase the frequency of contractions, whereas at higher doses it inhibits cholinergic-induced
contraction of the ruminal muscles or acts as an agonist to inhibit contractions in sheep. Hence, proglumide at high doses
seems unsuitable for research or therapeutic use as a CCK receptor blockade in sheep. 相似文献
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Onaga T Sugita A Wakaiki R Hara I Kagawa K Kirisawa R Hayashi H 《Domestic animal endocrinology》2008,35(2):231-244
The present study examined localization of cholecystokinin receptor (CCK-R) mRNA in the muscle layer of the ovine omasum and role of CCK-R type 1 (CCK-1R) in the regulation of muscle contraction of the omasum. We demonstrated that not only CCK-R type 2 (CCK-2R) mRNA but also CCK-1R mRNA is highly expressed in the muscle layer of the ovine omasum. Application of CCK-8 to muscle strips of the greater curvature of the ovine omasum at 1-100 nM induced tonic contraction in a concentration-dependent manner, and the contractile effect of CCK-8 was inhibited by both CCK-1R antagonist lorglumide (IC(50) 2.7 and 7.9 microM in the longitudinal and circular muscle, respectively) and CCK-2R antagonist PD135,158 (IC(50) 51.4 microM in the longitudinal muscle), indicating that not only CCK-2R but also CCK-1R is functionally expressed in the plasma membrane of smooth muscles in the omasum and mediates action of exogenous CCK. Contractile effect of intravenous infusion of CCK-8 (1-30 pmol/kg/min) on omasal contraction was also confirmed in the in vivo experiments using conscious sheep in the absence and presence of atropine infusion (14.4 nmol/kg/min), and showed that circulating CCK increases omasal electromyographic (EMG) activity at lower plasma concentration than that it inhibits ruminal contractions. Taking account of our previous results in the in vivo study using other CCK-1R antagonist, it is suggested that circulating CCK, even at normal range of plasma concentration, plays a physiological role as a regulator of omasal contractions in sheep and CCK-1R mediates the action of CCK. 相似文献
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本实验克隆了大菱鲆趋化因子受体基因CCR3和CCR9的全长cDNA, 并进行了鉴定。其中, 全长cDNA的克隆采用了5′和 3′-RACE。CCR3 cDNA全长1 451 bp, 包括92 bp的5′非编码区, 276 bp的3′非编码区以及编码360个氨基酸的1 083 bp的开放阅读框。CCR9 cDNA全长1 441 bp, 包括59 bp 的5′非编码区, 278 bp 的3′非编码区, 以及编码367个氨基酸的1 104 bp的开放阅读框。两种受体均具有7次跨膜结构, 符合G蛋白偶联受体的序列特征。系统进化分析表明, 大菱鲆CCR3与其他鱼类CCR3聚为一支, 大菱鲆CCR9也与其他鱼类的CCR9聚为一支; 系统进化树显示的亲缘关系符合各物种的进化地位。实时定量 PCR (qRT-PCR)表明这两个基因在大菱鲆正常组织中均有一定量的表达, 尤其是它们在头肾、脾及心脏中均有高水平的表达。在脂多糖(Lipopolysaccharide, LPS)诱导之后, CCR3在脾和肝中的表达水平与对照组有显著性差异(P<0.05), 而CCR9的表达水平仅在肝中与对照组有显著性差异(P<0.05)。两个基因在免疫相关组织中表达水平较高, 并且能够对LPS产生免疫应答, 说明它们在大菱鲆免疫系统中发挥了重要功能。
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T. Ostaszewska K. Dabrowski M. Kamaszewski K. Kwasek M. Grodzik J. Bierla 《Aquaculture Nutrition》2013,19(1):100-109
The aim of the present study was to evaluate the effects of three formulated diets: wheat‐gluten‐protein‐based diets supplemented with Lys‐Gly dipeptide (LG) or free lysine and glycine (FL), a control diet without lysine supplementation (C) and commercial starter Bio Oregon (BO) for on the growth and digestive system morphology of yellow perch. After 48 days of experimental feeding, fish fed LG diet showed the highest body mass. Fish fed LG diet showed the highest number of gastrin/cholecystokinin positive cells and the lowest number of CD3‐positive cells. The brush border of anterior intestine was the most PepT1 immunopositive in fish fed LG diet, the weakest in fish fed C diet. The largest hepatocytes were observed in fish fed BO, while the smallest in those fed FL diet, the difference being statistically significant. Relative hepatocyte cytoplasm volume occupied by lipids was higher in fish fed BO and FL compared to those fed C and LG. Number of proliferating cell nuclear antigen‐positive hepatocyte nuclei did not significantly differ among experimental groups. These results indicate that wheat‐gluten‐protein‐based diets supplemented with dipeptide Lys‐Gly (LG) were appropriate for yellow perch. 相似文献