甜菜BvGSTU9基因的生物信息学及在镉胁迫下的表达分析     

周婉婷  汪曼  周翔  高卓  李佳佳  李王胜  李思琪  王雪倩  王录红  刘大丽 《中国农学通报》2021,37(36):111-118

为了进一步研究甜菜谷胱甘肽转移酶BvGSTU9 (LOC104894060)在重金属胁迫过程中的功能。本研究以‘780016B/12优’为实验材料,对该基因序列特征、结构、功能进行预测分析,并利用qPCR检测该基因在不同浓度镉胁迫下的表达量变化。结果显示甜菜BvGSTU9基因全长925 bp,开放阅读框675 bp,编码了由224个氨基酸组成的不稳定膜外蛋白。BvGSTU9与菠菜、藜麦的氨基酸序列相似性较高,与系统发育进化树分析结果基本相符。二级和三级结构预测表明该基因主要由α-螺旋、β-折叠、延伸链及无规则卷曲组成。qPCR显示BvGSTU9基因在不同浓度的镉胁迫下均受到不同程度的诱导,因此可以推断甜菜BvGSTU9基因无论从结构还是功能上,与镉逆境胁迫存在着一定的应答关系。研究结果也为甜菜耐重金属镉机制研究提供参考依据。  相似文献   

Measuring trail users’ perception of crowding in a peri-urban nature park: A best-worst scaling experiment     

《Urban Forestry & Urban Greening》2018

This paper evaluates the perception of crowding, its determinants and relationship with trail users’ experience in a peri-urban nature park. Existing crowding surveys rely heavily on a single-item 9-point crowding scale which was originally developed for backcountry areas. Receiver operating characteristic (ROC) analysis was used to test the validity and reliability of this measure in detecting crowding issues and formulating visitor capacity limits. Low validity and reliability raised concerns about the applicability of this scale in similar frontcountry areas. Inability of this simple crowding measure to detect a visitor threshold brings to the fore its another important limitation – ignorance of the complexity of crowding phenomenon. The perception of crowding varies with various social, psychological and situational factors, and a best-worst scaling (BWS) experiment was designed to simultaneously evaluate different aspects of crowding in the Medvednica Nature Park, a peri-urban nature park near Zagreb, Croatia. The findings of BWS experiment revealed that occupancy of mountain huts, number of other hikers on the trail and amount of litter along the trail on average contributed most to the trail users’ sense of crowding. A latent class analysis identified a large heterogeneity in the perception of crowding; while one group of trail users (63%) was more disturbed by the direct social impacts, the others (37%) were more disturbed by the secondary impacts of other visitors, especially by the negative externalities from road traffic in the park. Older visitors had a greater tendency to primarily associate crowding with the environmental conditions. A higher impact of trail use level in the perception of crowding, younger age and more frequent use were associated with a more negative evaluation of crowding. The park management should closely monitor road traffic in the park and visitor activity on highly used trails, especially on those popular among younger trail users.  相似文献   

S195—1型低背压排气消声器的设计研究     

李梅林 《湖南农业大学学报(自然科学版)》1990,17(3)

本文以内燃机排气噪声必需的消声量频率特性作为设计消声器的依据,论述了扩张室式和穿孔板复合消声器的设计.试验表明,采用这种新型复合消声器后,S195柴油机排气噪声得到了有效控制.  相似文献   

草坪恶性杂草蟋蟀草的发生生物学——蟋蟀草发生对播期的反应     

李善林  韩烈保 《中国草地学报》2001,23(2):46-48

在北京地区蟋蟀草从5月开始发生，可以持续到10月底，6月、7月出土速率和出土率强于其它月份，9月后出土率显著下降；5～10月期间生长的蟋蟀草，其种子与植株的生物学产量比率为5～7的值占64%。脱离母株的蟋蟀草种子当月存在萌发力，半年后萌发率达到60%。  相似文献   

辣椒黄绿苗突变体生物学特性及生长动态研究   总被引：9，自引：0，他引：9  

马志虎  颜素芳  胡志中  郝华忠  丁金贵 《种子》2001,(4):10-12

对96－140牛角辣椒及96－140辣椒黄绿苗突变体（96－140YBM）生物学特性、生长动态、抗病性等方面进行了测定，试验收结果表明96－140辣椒黄绿苗突变体不仅在生长势，叶片、株重、单果重等方面强于96－140，而且在抗疫病方面明显强于96－140牛角辣椒。96－140YBM性状遗传稳定，苗期遗传标记性状明显，在杂交育种及纯度鉴定上有较大的利用价值。  相似文献   

The Effect of Nitrogen and Phosphorus Fertilization on the Nitrogen Absorption by Sunflowers     

H. L. Loubser  J.J. Human 《Journal of Agronomy and Crop Science》1993,170(1):39-48

Little is known about the effect of fertilization on the N uptake of sunflowers. A 4² factorial trial with 0, 60, 120 and 180 kg N ha⁻¹ and 0, 15, 30 and 45 kg P ha⁻¹ was conducted over three years. The N content and concentration of leaves, stems and capitula were determined at three growth stages. High N levels increased the N content and concentration of all plant parts at all growth stages sharply. High P levels increased the N content of all plant components through better growth. P has an inconsistent effect on N concentration but tended to decrease it. After flowering the crop assimilated 20 to 25 % of the total N. This implies that N applied can still be applied and utilized by the crop at a late stage. This should be substantiated by further research.  相似文献   

Die Wurzelentwicklung von Zuckerrübenpflanzen auf einem Sandlöß-Standort und ihre Bedeutung für die Stickstoffernährung     

J. Garz    M. Abdallah  W. Schliephake 《Journal of Agronomy and Crop Science》1992,169(4):260-269

Root development of sugar beet plants on a sandy loess site with regard to nitrogen nutrition.
Root development of sugar beet plants in a sandy loess soil (Haplic Phaeozem) was observed from the early seedling stage up to harvest by measuring at first the greatest vertical and lateral extension of the root systems of single plants and later the rooting density of the whole plant stands (auger method, profile wall method).
During the seedling stage not only the subsoil, but also large parts of the topsoil between the plants remained unoccupied by the root systems. In this phase the greatest lateral extension of single roots reaches nearly the length of the greatest leaf of the plant. With the closure of the canopy the rooting density in the topsoil accounts to 1–2 cm cm⁻³.
In summer roots penetrate to a depth of 100–150 cm with rooting densities of 0.1 to 1 cm - cm⁻³. Thus, the plants gain not only access to water reserves, but sometimes meet remarkable amounts of nitrate which under the relatively dry conditions of the region tends to accumulate in 60–120 cm depth and – when taken up by the beet plants in the late stage of growth – affects crop quality negatively.  相似文献   

退耕还林间作万寿菊的高产栽培技术     

冯技巧  李清林 《林业科技情报》2006,38(2):8-9

本文介绍了万寿菊的形态特征及生物学特性,根据形态特征及生物学特性,探索了万寿菊的育苗技术和栽培技术以及田间管理的方法。  相似文献   

盐分对碱茅种子发芽影响的机制   总被引：15，自引：0，他引：15  

阎顺国 Bake.  DA 《草地学报》1994,2(2):12-19

耐碱茅种子在盐（NaCl与Na2SO4）及干旱（PEG－6000模拟）胁迫下发芽及其机制的比较研究表明，种子发芽率随溶液渗透势的降低而下降，在等渗条件下三种溶液中的发芽率次序为Na2SO4＞NaCl＞PEG。NaCl与Na2SO4主要通过影响种子吸水量及对膜的毒性影响发芽，根据相关及通径分析结果，其机制为：（1）溶液渗透势及Na+浓度决定的种子吸水对发芽的直接正效应（通径系数0．73）；（2）溶液Na+浓度引起的膜毒性对发芽的直接负效应（－0．51）；（3）种子从溶液中吸收Na使种子吸水增加对发芽的间接正效应（0．30）及使膜毒性增大对发芽的间接负效应（－0．24）。  相似文献   

血清8型鸭疫里墨氏杆菌在我国的发现及其病原特性研究   总被引：10，自引：3，他引：7  

汪铭书  程安春  陈孝跃  刘兆宇  方鹏飞  郭宇飞 《中国预防兽医学报》2003,25(1):47-52

1994年1月-2000年10月，从全国23个省（市，自治区）不同代次（原种，祖代，父母代和商品代）的5-90日龄患有典型鸭传染性浆膜炎的病死鸭分离到68株血清8型的鸭疫里墨氏杆菌，对其病原学特性进行研究的结果表明，血清8型的鸭疫里墨氏杆菌在我国鸭群感染分布的范围极广，分离菌株对雏鸭具有很强的致病性，对小鼠无致病性，各地分离菌株表现出一致的形态特征和非常相似的生理生化特性；各地分离菌株耐药谱很广，但对头孢类药物（如头孢拉啶，头孢克洛等）和利福平却普遍高度敏感。用分离菌株制备的灭活疫苗免疫雏鸭具有良好的免疫原性。  相似文献