全文获取类型
收费全文 | 195篇 |
免费 | 83篇 |
国内免费 | 16篇 |
专业分类
林业 | 3篇 |
农学 | 4篇 |
4篇 | |
综合类 | 173篇 |
农作物 | 2篇 |
水产渔业 | 1篇 |
畜牧兽医 | 65篇 |
园艺 | 39篇 |
植物保护 | 3篇 |
出版年
2023年 | 4篇 |
2022年 | 6篇 |
2021年 | 11篇 |
2020年 | 2篇 |
2019年 | 11篇 |
2018年 | 18篇 |
2017年 | 59篇 |
2016年 | 28篇 |
2015年 | 7篇 |
2014年 | 10篇 |
2013年 | 7篇 |
2012年 | 28篇 |
2011年 | 32篇 |
2010年 | 15篇 |
2009年 | 5篇 |
2008年 | 9篇 |
2007年 | 4篇 |
2006年 | 8篇 |
2005年 | 3篇 |
2004年 | 4篇 |
2003年 | 7篇 |
2002年 | 4篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1996年 | 1篇 |
1992年 | 2篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有294条查询结果,搜索用时 16 毫秒
1.
2.
3.
HEI Zi-qing WU Wei-kang SUN Hui-lan HUANG He-qing TAN Hong-mei LUO Han-chuan 《园艺学报》2003,19(3):345-347
AIM:To observe the effects of Sini decoction on atherosclerosis(AS) and ceramide content of aorta in rabbits. METHODS:28 rabbits were randomly divided into three groups. Control group was fed with a normal diet; High cholesterol group was fed 1% cholesterol and 5% fat diet; Sini decotion+ high cholesterol group was fed 1% cholesterol and 5% fat diet plus Sini decotion (4.2 g·kg-1·d-1). At the end of study, the plaque area were measured, the atorta ceramide and cell apoptosis were also detected. RESULTS:Sini decotion diminished lipid plaque area on the aortic endothelium, reduced the levels of aorta ceramide and the apoptosis index. CONCLUSION:Sini decoction has an inhibitory effect on AS, the mechanism may be that Sini decotion reduces concentration of ceramide in aorta. 相似文献
4.
XUE Ying-sheng WANG Jing-feng NIE Ru-qiong WU Wei-kang LUO Han-chuan CHEN Xiao-wei 《园艺学报》2004,20(4):603-607
AIM: To investigate the influence of Sini decoction (SND) on the proliferation and apoptosis of rabbit abdominal aorta smooth muscle cells after ballon injury and discuss the effect of vascular smooth muscle cell's (VSMCs) proliferation and apoptosis in post-percutaneous coronary intervention (PCI) restenosis (RS) and the feasibility of SND preventing post-PCI RS. METHODS: The animal model of rabbit abdominal aorta ballon injury was set up and the therapertic group was treated with SND. The shape of proliferative and apoptotic cell were investigated by electron microscope. Immunohistochemistry staining was performed using α-actin,PCNA and Cyclin E monoclonal antibodies. In situ Cell Death Detection Kit was used to identify apoptotic cells. Abdomial aorta angiography was operated in the 84th day subgroup and the stenosis degree was evalued by quantitative angiographic analysis. RESULTS: As compared with the control group, the therapeutic group displayed a lower proliferative percentage and a higher apoptosic percentage (P<0.05). Moreover, the apoptosic peek time was on the 14th day after operation,which was longer than the control group. CONCLUSION: SND effectly inhibited the proliferation of VSMCs and iuduced apoptosis in VSMCs. 相似文献
5.
AIM: To investigate the effect of Sini decoction (SND)on the ultrastructure of small intestinal epithelial cells in rats with intestinal ischemia-reperfusion. METHODS: Thirty-two Sprague-Dawley rats of both sexes were randomly divided into 4 groups: (1) Control group in which sham operation was performed; (2) Model group in which intestinal I/R was produced by clamping super mesenteric artery(SMA) for 1 hour and declamping SMA for 3 hours; (3) SND1 group in which SD (0.6 g/200 g rat) was given via stomach tube 3 d before intestinal I/R; (4) SND2 group in which SD (1.2 g/200 g rat)was given via stomach tube 3 d before intestinal I/R. A strip of small intestine was taken from distal end of ileum for electron microscopic examination. The two-dimensional structural parameters and three-dimensional structural parameters of mitochondria were calculated. RESULTS: (1)Morphological changes of small intestine: In control group, epithelial cells were orderly arranged, with normal mitochondria and intestinal villi. In model group, the gaps between epithelial cells widened. There were a lot of apoptotic cells. Microvilli were short and swelled. Mitochondria were swelled obviously with broken ridges. Endoplasmatic reticulum was severely dilated. In SND1 and SND2 groups, microvilli and epithelial cells were orderly arranged relatively, mitochondria was slightly swelled. (2) Structural parameters of mitochondria: In model group, there were the least mitochondria and the swelling of mitochondria was severe. In SND1 and SND2 groups, the mitochondria was more than that of model group and the swelling were slight. CONCLUSION: Sini decoction can protect small intestine from ischemia-reperfusion injury without dose-dependent effect. 相似文献
6.
15头健康仔猪,分3组,每组5头:即健康组、脾虚组和四君子汤反证组。按20mg/kg的剂量内服进行左旋氧氟沙星的辨证药动学研究。高效液相色谱法测定血浆中药物浓度,3p97药代动力学程序处理药时数据。健康组和脾虚组药动学数据符合一级吸收一室模型,四君子汤反证组药动学数据符合一级吸收二室模型。试验结果表明:仔猪脾虚状态下明显影响左旋氧氟沙星内服给药的药动学特征(PK);四君子汤可使脾虚仔猪异常的左旋氧氟沙星的药动学特征得到恢复,为验证“辨证药动学”假说和完善中西兽医结合提供了进一步的科学依据。 相似文献
7.
AIM To observe the effect of Fuzilizhong decoction on the inflammatory damage of non-alcoholic fatty liver disease (NAFLD) rats and to explore its mechanism. METHODS SPF male SD rats were randomly divided into 6 groups: control group, model group, high dose (20 mg·kg-1·d-1), middle dose (10 mg·kg-1·d-1), low dose (5 mg·kg-1·d-1) Fuzilizhong decoction group and Yishanfu (30 mg·kg-1·d-1)group, 8 rats in each group. A NAFLD rat modelwas established by intragastric administration of fat emulsion for 4 weeks. Then the drug was given for 4 weeks in each treatment group. HE staining was performed to observe the histopathological changes of the rat liver.The serum levels of interleukin-2(IL-2), IL-6 and tumor necrosis factor-α(TNF-α) were measured by ELISA. The expression of toll like receptor 4(TLR4) and NF-κB p65 in liver tissues at mRNA and protein levels was determined by RT-qPCR and Western bolt,respectively. RESULTS Compared with control group, the inflammatory damage of liver tissue was more serious, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression TLR4 and NF-κB p65 in liver tissues were significantly increased in model group(P <0.05). However, compared with model group, the liver pathological changes in each treatment group were significantly relieved, the serum levels of IL-2, IL-6 and TNF-α, the mRNA expression of TLR4 and NF-κB p65 in liver tissues were significantly reduced(P <0.05).In addition, the changes of TLR4 and p-NF-κB p65 protein levels in liver tissue were consistent with the changes of TLR4 and NF-κB p65 mRNA. CONCLUSION Fuzilizhong decoction attenuates the inflammatory damages of NAFLD in rats by inhibiting TLR4/NF-κB p65 signaling pathway. 相似文献
8.
9.
实验性脾虚证大鼠肝脏和肌肉组织中糖原变化的组织学观察 总被引:4,自引:0,他引:4
将45只大白鼠随机分成对照组、脾虚模型组及治疗组,每组15只。以利血平(1mg/kg·d)复制大白鼠脾虚证模型,四君子汤辅助治疗,在实验进行至第7天和第14天分别捕杀各组大白鼠,取肝脏、肌肉组织,用组织化学方法测定在不同组间和不同实验阶段其中糖原的变化。结果,在第7天时,脾虚组和治疗组糖原含量明显低于正常对照组,第14天时,与正常对照组相比较,脾虚组和治疗组糖原含量显著升高,且脾虚组糖原含量较其它两组高,提示肝脏和肌肉组织中糖原的变化可能是脾虚证发生发展的重要指标。 相似文献
10.
四君子汤对仔猪胸腺及脾脏生长激素受体mRNA表达的影响 总被引:1,自引:0,他引:1
选用90头新生"杜×长×大"三元杂交仔猪,随机分为3组,每组30头,分别为中药I组(中药添加剂量10 g/kg),中药Ⅱ组(中药添加剂量20 g/kg),对照组(基础日粮组),试验期为31 d.45日龄时每窝随机选取6头仔猪屠宰后,分别采集胸腺和脾脏.采用反转录聚合酶链式反应(RT-PCR)方法,定量分析仔猪胸腺及脾脏中生长激素受体(GHR)mRNA的相对丰度.结果显示,与对照组相比较,2种剂量中药组的仔猪胸腺及脾脏中生长激素受体mRNA的含量均不同程度地提高,中药Ⅱ组效果更为显著. 相似文献