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The aim of this trial was to assess the effect that calcium gluconate priming of 468 broilers has on the antibacterial activity of a standard dose of enrofloxacin. Hence, a series of oral pharmacokinetic studies were carried out in four groups of broilers medicated individually through an oral cannula as follows: group A, medicated only with enrofloxacin 10mg/kg; group B, receiving immediately one after the other, calcium gluconate (200mg/kg) and enrofloxacin 10mg/kg; group C, dosed first with calcium gluconate (200mg/kg) and 1h later enrofloxacin (10mg/kg); and group D, dosed first with calcium gluconate (200mg/kg) and 2h later enrofloxacin (10mg/kg). Broilers were bled at different times after the dose of enrofloxacin and antibacterial activity, measured as concentration of enrofloxacin, was measured by an agar diffusion assay. Results revealed that group D the greatest values of maximum serum concentration (Cs(max)), area under the concentration vs. time curve (AUC) and area under the moment curve (AUMC). These values were statistically higher than the corresponding ones derived from groups A, B and C (P<0.05). Taking Cs(max) and AUC values of group A as reference baseline, an increase of 24% and 50%, respectively, was obtained in group D. Group B had the lowest Cs(max), AUC, AUMC and elimination half life (T(1/2)beta) and these values were statistically different from groups A, C and D (P<0.05). The T(1/2)beta was statistically longer in groups C and D as compared with A and B, and the former groups were also different between each other (P<0.05). These results show that if calcium gluconate is first dosed to broilers and 2h later enrofloxacin is administered (as in group D), a more pronounced antibacterial activity of enrofloxacin can be obtained. A challenge of this sequential dosing scheme in a field trial may reveal its clinical value. 相似文献
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西部民族贫困山区是我国扶贫攻坚的重点和难点地区,其扶贫工作的好坏直接关系：我国民族团结、全面建设小康社会目标的实现。在分析西部民族贫困山区县——四川省昭觉县农村贫困与扶贫工作现状的基础上,总结了适合民族贫困山区县的扶贫模式——“三集中”扶贫模式,提出通过发展县域循环经济和建立健全生态环境补偿机制来推广发展“三集中”扶贫模式的对策建议。 相似文献
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《Communications in Soil Science and Plant Analysis》2012,43(1-2):88-94
This article examines the metal [aluminum (Al), iron (Fe), manganese (Mn), copper (Cu), and zinc (Zn)] runoff dynamics in the Mero River (northwestern Spain). At the catchment outlet, metal concentrations, suspended solids, dissolved organic carbon, pH, and discharge were determined during three runoff events. The river drains an agroforestry catchment of 65 km2 with relatively high livestock density. Dissolved and particulate metal concentrations increased during the events with respect to pre-event concentrations, but the increase of the dissolved fraction is relatively small compared to that of the particulate fraction. The dissolved metal concentration peaks appeared after the hydrograph peak, suggesting transport associated with subsurface flow. For particulate metals, peaks usually occurred during the falling limb of hydrograph, implying distant-river source of metals. Particulate forms represented more than 90% of total Al, Fe, and Mn load, whereas for Cu and Zn its contributions were 52–76% and 31–56%, respectively. The high positive correlations of all particulate metals, except Zn, with suspended solid concentrations indicate that these constituents play a major role in transport of metals. Soil erosion is the main process responsible for causing elevated Al, Fe, and Mn concentrations in the river during rainfall–runoff events while Zn and Cu come mainly from the addition of slurries and manures to farmland. 相似文献
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不同氮磷浓度对小球藻生长性能的影响 总被引：1，自引：0，他引：1
[目的]研究小球藻在不同氮源及氮磷浓度的条件下的生长状况。[方法]以小球藻为试验材料,采用紫外分光光度法、生物量及最大比生长速率来表征小球藻在不同条件下的生长情况,研究不同氮源及氮磷浓度对其生长性能的影响。[结果]小球藻的最适氮源为硝态氮,当氮浓度为164.0 mg/L时,小球藻的生长速度较快,经过10 d的培养,小球藻的生物量及最大比生长速率分别为97.26 mg/L和0.32 d-1;当磷浓度为360.0 mg/L时,小球藻的生长速度较快,其生物量和最大比生长速率分别为98.46 mg/L和0.31 d-1,显著高于其他磷浓度的处理。 相似文献
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研究了不同激素种类与含量及复配处理对F1代草花苗开花及植株高度的影响。结果表明:较低含量的激素(IBA50、100μg·g-1、NAA50、100μg·g-1)和复配处理(IBA50μg·g-1 NAA150μg·g-1)可明显提前F1代草花苗的花期(2～4d),不同激素种类与含量对花序数和花朵数无明显影响,对花序长或花朵直径无明显的增加效果。不同激素种类与含量处理下的植株高度出现明显增长的现象,但并不影响F1代草花苗的商品价值。 相似文献
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培养基组分对四倍体水稻试管速繁中不定芽丛增殖的影响 总被引：1，自引：0，他引：1
以 MS 培养基为基础,研究了诱导四倍体水稻体细胞试管苗形成及其繁殖的几个因素。(1)诱导四倍体水稻试管苗(芽)大量形成不定芽的必要条件是 KT≥11.57mg/l(KT/NAA≥1000),获得最大不定芽丛生速率的 KT 水平为23.20mg/l(KT/NAA=2000)。(2)氮、磷水平对不定芽的诱导及增殖速率均有明显的影响,它们的最佳水平分别为10100mg/l KNO_3+1320mg/l(NH_4)_2SO_4(纯总氮量120mmol/l。NO_3~--N/NH_4~+-N=5)和1190mg/l KH_2 PO_4。(3)继代培养第十天添加 CLNⅡ补充营养液(23.20mg/l KT.0.01mg/l NAA,MS 有机附加物及铁盐.800mg/l水解乳)能够显著地提高不定芽的丛生速率。(4)蔗糖利用率和激素水平密切相关,其在23.20mg/l KT+0.01mg/l NAA 条件下达到最大值(85.5%)。 相似文献
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《Communications in Soil Science and Plant Analysis》2012,43(10):989-1004
Abstract The effects of application of zinc fertilizer on dry matter yield, uptake and distribution of zinc and other nutrients by Amazon and Amelonado cocoa cultivars grown in a soil of low zinc content in the greenhouse were investigated. There was a differential response to zinc fertilization by the two cocoa cultivars. Maximum dry matter yields of Amazon and Amelonado were attained with 10 ppm Zn and 50 ppm Zn, respectively. Under similar experimental conditions Amelonado seedlings expressed zinc deficiency symptoms whereas Amazon did not. Zinc concentrations in the leaves, stem and roots of both cuitivars did not give a good index of the zinc status of the crops. This was because of the existence of the “Piper‐Steenbjerg”; effect in that nil zinc rates often gave higher leaf concentration of the zinc than next higher rate. In general, the relative content of zinc followed the pattern; leaves > roots > stem with the Amazon cultivar containing more zinc than the Amelonado. The distribution of absorbed Cu in the leaves, stem and roots did not differ in both varieties. Whereas Fe uptake was mostly concentrated in the roots, Mn absorbed was largely concentrated in the leaves of both varieties and only Mn uptake in the leaves of Amazon consistently increased with Zn application. The differences in the uptake and distribution of nutrients between the two cocoa cultivars was attributed to differences in their ability to extract nutrients from the soil and in their requirements for metabolic processes. 相似文献
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浙江省茶园土壤锰含量状况研究 总被引：9，自引：2，他引：9
2002年对浙江省部分茶园锰调查结果表明,浙江省茶园全4锰含量0～20cm土层,平均为357.33±265.26mgkg-1,20～40cm土层,平均为361.69±268.69mgkg-1。有效锰含量0～20cm土层,平均为51.16±30.16mgkg-1,20～40cm土层,平均为38.32±25.85mgkg-1,约占全锰含量的15%左右,茶园缺锰率约占30%左右。从上下土层来看,表现出上层锰含量要比下层高。茶树吸收锰与土壤有效锰含量呈极显著正相关,随着叶片的成熟度增加,锰积累增加,同时茎干部也是锰累积的部位。土壤有效锰含量与土壤pH、Al、成土母质有关。 相似文献
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以柠条锦鸡儿(Caragana korshinskii Kom.)无菌苗的子叶和下胚轴为外植体,研究其愈伤组织诱导及植株再生。结果表明:柠条锦鸡儿子叶和下胚轴均能诱导出愈伤组织,其适宜诱导培养基分别为M S+2,4-D2.0(mg/L)+KT0.1(mg/L)和M S+2,4-D2.0(mg/L)+BA0.5(mg/L);子叶诱导不定芽的适宜培养基为M S+6-BA0.2(mg/L)+NAA0.05(mg/L),下胚轴诱导不定芽的适宜培养基为MS+6-BA0.5(mg/L)+NAA0.05(mg/L);适宜生根培养基为1/2M S+IBA0.5(mg/L)+NAA0.5(mg/L)。本试验结果可为柠条锦鸡儿的快速繁殖利用及品种改良提供依据。 相似文献