糙皮侧耳和佛罗里达侧耳菌丝原生质体的形成与再生     

贺建超  石国昌 《中国食用菌》1989,(5):13-14

本文报道了使用蜗牛酶和纤维素酶从糙皮侧耳和佛罗里达侧耳双核菌丝体中,成功地分离了高产量的原生质体。菌丝体培养时间,混合酶液 pH 值,一定浓度的酶液用量,酶解温度和时间与原生质体形成相关。原生质体的两种再生型被观察到,并计算了再生频率,糙皮侧耳为2.5%.佛罗里达侧耳为2.3%。  相似文献   

处理主干和主枝对光皮树幼树早花早实的促进效果     

向明  蒋丽娟  李昌珠  张良波  李培旺  姚茂华 《湖南林业科技》2011,38(3):20-23,44

对3年生光皮树幼树,采用环割倒贴皮、铁丝绞缢、绳索绞缢、环刻4种人工造伤方法分别对其主干和主枝进行处理.结果表明:4种处理方法对树干直径和冠幅生长的影响差异不显著;铁丝绞缢主枝法处理,其树高及开花株数和开花数量都与其他处理存在显著差异;铁丝绞缢主枝与铁丝绞缢主干法促进开花的效果差异不显著.  相似文献   

‘芽黄’红瑞木炭疽病病原菌的分子鉴定     

罗卿权 《植物保护》2020,46(5):102-109

‘芽黄’红瑞木炭疽病在上海普遍发生。本研究从上海迪士尼园区采集疑似炭疽病的红瑞木病叶,进行了分离培养,获得2种培养形态的炭疽菌,致病性测定证明其为致病菌。联合核糖体转录间隔区、3-磷酸甘油醛脱氢酶、钙调蛋白和β-微管蛋白2多基因序列,使用最大似然法和贝叶斯分析法分别构建了多基因系统进化树。结果显示,分离获得的2株炭疽菌菌株分别与暹罗炭疽菌Colletotrichum siamense和胶孢炭疽菌C.gloeosporioides聚集在一个分支,辅以形态学特征,鉴定引起‘芽黄’红瑞木炭疽病的病原菌为暹罗炭疽菌和胶孢炭疽菌,这是国内关于红瑞木炭疽病的首次报道。  相似文献   

基于文献计量学的山茱萸研究现状分析     

陈淑慧  纵伟 《现代农业科技》2020,(6)

山茱萸具有降血糖、抗氧化、抗肿瘤、抗菌等功能作用,开发山茱萸资源具有重要意义,其研究受到广大科技工作者的关注。为了解近10年来山茱萸的研究现状,本文采用文献计量学方法,以中国学术期刊网络出版总库(CNKI)2009—2018年的数据为检索对象,从年度分布、研究机构、学科分类、期刊来源等方面分析山茱萸研究文献,以期为研究者提供参考。  相似文献   

山茱萸多糖提取工艺优化及马钱苷含量测定     

常玲彬 《甘肃农业科技》2011,(11):26-29

采用L（934）正交设计试验,对山茱萸浸提液中山茱萸多糖的酶水解法提取工艺进行了优化研究,并对浸提液的中有效成分马钱苷含量进行了HPLC法分析。结果表明,山茱萸多糖浸提的最佳工艺为：液料比1∶5,浸提时间4 h,浸提温度80℃,果胶酶添加量0.55 g/L。用HPLC法测定出的山茱萸浸提液中马钱苷平均含量为0.512 ...  相似文献   

Effect of salt stress on the physiological and photosynthetic characteristics of Weigela florida     

Qingjuan Nie  Zhigang Wang  Zhibin Ren  Dazhuang Huang 《Frontiers of Agriculture in China》2011,5(4):655-661

Effects of salt stress on the physiological and photosynthetic characteristics of Weigela florida were studied. The results showed that the leaf area of Weigela florida was enhanced at 0.1% and 0.2% salt concentration, but decreased obviously when salt concentration was higher than 0.3%. The symptom of the salt injury was not significant when the salt concentration was lower than 0.3%, but was significant when higher than 0.4%. The water content decreased gradually but the electrolyle leakage increased gradually with the increasing of salt concentration and time. The total content of chlorophyll was rising while salt concentration was lower than 0.3%, but decreased while salt concentration was higher than 0.4%. The proline contents increased gradually while salt concentration was lower than 0.5%, but decreased at 0.5%. The soluble sugar content increased gradually with the increasing of salt concentration and prolonging of treatment, but decreased at 0.4% salt concentration 15 days after salt treatment. To the photosynthetic characteristics, the salt concentrations of 0.1% and 0.2% did not affect the photosynthetic characteristics of Weigela florida, when salt concentration was higher than 0.3%, the salinity significantly reduced Pn, Gs and Tr, but enhanced intercellular CO₂ concentrations at the salt concentrations of 0.3% and 0.4%. It was indicated that 0.1% and 0.2% salt concentrations had little influence on Weigela florida. The growth of Weigela florida was slightly decreased at the salt concentration of 0.3% without effect on its normal growth. However, the salt concentration of 0.4% affected the growth of Weigela florida obviously. Weigela florida died 7 days after treatment and it could not normally grow when salt concentration is higher than 0.5%.  相似文献   

山茱萸不同部位提取物对α-葡萄糖苷酶的抑制作用     

张夙夙 《安徽农业科学》2014,(23):7741-7745,7747

[目的]测定山茱萸果肉和果核提取物对α-葡萄糖苷酶的抑制作用,为山茱萸资源的药用开发提供参考。[方法]采用乙醇和水分别对山茱萸果肉和果核进行提取,并测定其对α-葡萄糖苷酶的抑制作用。[结果]山茱萸果核提取物对α-葡萄糖苷酶的抑制率分别为20倍醇提99.23%、30倍醇提98.96%、40倍醇提99.29%、20倍水提99.36%、30倍水提99.32%、40倍水提99.17%。山茱萸果肉提取物对α-葡萄糖苷酶的抑制率分别为20倍醇提52.10%、30倍醇提60.77%、40倍醇提21.67%、20倍水提11.88%、30倍水提0.53%、40倍水提1.59%。[结论]山茱萸不同部位的水提取物和乙醇提取物对α-葡萄糖苷酶均有一定的抑制作用,其中山茱萸果核部位对α-葡萄糖苷酶的抑制作用最为显著,其抑制率可高达99%以上,可见山茱萸果核中含有丰富的抑制α-葡萄糖苷酶活性的物质,可作为药用资源予以开发利用。  相似文献   

多花梾木种子发芽特性研究     

周余华  唐义明  赵明明 《安徽农业科学》2014,(28):9809-9810

[目的]研究多花株木种子的发芽特性.[方法]采用正交试验设计,研究在不同温度、不同酸蚀时间、不同GA3浸种浓度对种子发芽率的影响.[结果]温度对种子发芽影响较明显,在5℃低温处理下时长100 d发芽率最好,达72％.[结论]低温处理在一定条件下能提高多花株木种子的萌发能力.  相似文献   

干旱胁迫对4个锦带花品种叶绿素及PSⅡ光化学活性的影响     

海小霞  吕飞  郑素珊  刘炳响  王志刚  聂庆娟 《东北林业大学学报》2014,(11)

为了明确干旱胁迫对四季锦带、红王子锦带、紫叶锦带和金叶锦带4个品种叶绿素质量分数和荧光参数的影响,采用田间持续干旱法,测定了各参数的变化曲线。结果表明：在干旱胁迫下,各品种叶绿素质量分数变化规律较一致,均呈不规则波动,其中四季锦带和金叶锦带、红王子锦带和紫叶锦带叶绿素质量分数曲线的变化趋势两两相似;胁迫显著降低了PSⅡ单位面积有活性反应中心数量RC／CSo、光化学性能指数PI,A,B,S值;荧光诱导曲线中K点相对可变荧光（Wk）和J点相对可变荧光（Vj）的值均较对照不同程度增加;暗适应下初始荧光Fo 值、PSⅡ最大光化学效率Fv／Fm 值均呈现下降趋势。总之,干旱胁迫下4个锦带花品种PSⅡ光化学活性受到不同程度的抑制,四季锦带对干旱的响应最强烈,其次是紫叶锦带和金叶锦带,红王子锦带最弱。  相似文献   

Correlations of stomatal conductance with hydraulic, chemical and environmental variables in five urban tree species     

T. Brendon Johnson  Robert M. Aug  Craig D. Green  Ann J. W. Stodola  Joshua B. Olinick  Arnold M. Saxton 《Scientia Horticulturae》2001,90(3-4):305-320

Recent findings suggest that stomatal conductance (g_s) may be as closely linked to plant chemical variables as to hydraulic variables. To test this in an urban field setting, we examined seasonal g_s in relation to a number of plant and environmental variables in five temperate, deciduous tree species. Stomatal conductance was generally more closely correlated with abscisic acid concentrations in xylem sap than with shoot water potential, shoot osmotic potential, pH of xylem sap or environmental variables. Seasonal g_s was mostly poorly correlated with shoot water potential and osmotic potential. Among environmental variables, PPFD accounted for most variability in g_s. We tested a model, developed previously in maize, that describes regulation of g_s by abscisic acid concentration of xylem sap with leaf water status acting to modify stomatal sensitivity to the abscisic acid signal. This model explained somewhat more variation in g_s than abscisic acid concentrations alone. Response surface models, especially those incorporating environmental variables, were most successful at explaining g_s. Our findings with urban trees are consistent with the theory of regulation of g_s by root-sourced abscisic acid.  相似文献