甜瓜属新型作物--火参果的研究和利用现状及建议     

王吉明  尚建立  李娜  周丹  马双武 《中国瓜菜》2020,(4):1-6

火参果是我国近年来引进和栽培的新型瓜类水果作物,具有坐果能力强、果实外观漂亮、风味独特、经济效益高和发展前景广阔等优点。就火参果遗传多样性及分类地位、种质资源评价与栽培、抗性及其利用、营养和药用功能成分方面回顾了国内外研究进展,并对火参果栽培技术要点进行总结。此外,由于目前的火参果鲜食品质难以满足大众化的消费需求,市场空间较为有限,制约了火参果产业的进一步发展,提出了解决这个问题相关的建议,旨在为促进我国火参果产业发展提供借鉴。  相似文献   

不同稳定剂对胭脂萝卜红色素稳定性的影响     

芮春梅  李成琼  司军  宋洪元  任雪松  李利军 《勤云标准版测试》2006,(12)

天然胭脂萝卜红色素属于花青素类化合物,性质不稳定。笔者以新鲜胭脂萝卜(Raphnussativuscv.)肉质根为原料,提取胭脂萝卜红色素,选择常用的5种稳定剂对其稳定性进行研究,从而确定出最佳的稳定剂及其用量。研究结果发现0.03%维生素C、0.03%谷氨酸、0.03%柠檬酸能明显地延缓其颜色变化,0.03%维生素C 0.03%柠檬酸的组合对该色素的稳定效果最佳;进一步研究确定维生素C和柠檬酸的最佳用量分别为0.04%和0.08%。  相似文献   

甜瓜种质资源的判别分析   总被引：6，自引：0，他引：6  

张鲁刚  王鸣 《园艺学报》1992,19(1):35-40

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Transmission of the agent causing a melon yellowing disease by the greenhouse whitefly Trialeurodes vaporariorum in southeast Spain     

C. Soria  M. L. Gomez-Guillamon  J. E. Duffus 《European journal of plant pathology / European Foundation for Plant Pathology》1991,97(5):289-296

The agent causing a yellowing disease of melon (Cucumis melo), which results in severe losses in crops under plastic on the coastal plains of southeast Spain, was shown to be transmitted in a semipersistent manner by the greenhouse whitefly (Trialeurodes vaporariorum Westwood). The agent was transmitted by grafting, but not by mechanical inoculation or through seeds. The agent was acquired in the minimum period tested (2 h) and could infect plants in an infection feeding interval of 6 h. Capsella bursa-pastoris, Cucumis melo, C. sativus, Cucurbita moschata, Cichorium endivia, Lactuca sativa andTaraxacum officinale were found susceptible.Results suggest that the yellowing disease affecting melon crops in the southeast of Spain is due to a pathogen similar to beet pseudo yellows virus, but this has to be confirmed by serology.  相似文献   

黄瓜全雌性基因连锁的AFLP和SCAR分子标记   总被引：32，自引：5，他引：32  

娄群峰  陈劲枫  Molly Jahn  陈龙正  耿红  罗向东 《园艺学报》2005,32(2):256-259

 本研究以全雌品种‘戴多星’自交系和弱雌品种‘北京截头’自交系为双亲杂交获得F₁ ,然后得到F₂ 性型分离群体, 利用分离群体分组分析法(Bulked Segregant Analysis, BSA) 构建全雌和弱雌两个基因池, 筛选了64对AFLP选择性引物EcoR I-NN +Mse I-NNN组合, 发现EcoR I-TG +Mse I-CAC引物组合在全雌基因池中扩增出一条分子量为234 bp的特异带。经F₂ 代单株验证, 该特异条带能在全雌单株中稳定出现。以MAP MAKER (Version 310) 软件分析, 该标记与全雌性位点的连锁距离在617 cM。命名该连锁标记为TG/CAC₂₃₄。将该特异条带回收、克隆、测序, 设计特异SCAR引物, 再对F₂ 代单株基因组DNA进行扩增, 仅在全雌单株中扩增出1条分子量为166 bp 的特异带, 表明已成功地将与黄瓜全雌性连锁的AFLP标记转化为操作简便、表现稳定的SCAR标记, 该标记命名为SA₁₆₆。  相似文献   

从甜瓜子叶中提取总DNA   总被引：4，自引：1，他引：4  

陆璐  赵长增  陶兴林 《果树学报》2005,22(6):748-750

用分子标记方法检测甜瓜杂交种子纯度,如果从真叶中提取DNA,检测周期至少需要15d。为了缩短检测周期,我们分别以甜瓜的成熟干种子、吸胀水后“露白”种子、3d龄刚转绿子叶、6d龄子叶、9d龄子叶和12d龄子叶为材料,进行了提取总DNA的研究。结果表明:除了干种子和吸胀水后“露白”种子外,其他的材料都可以提取到DNA,但是DNA的质量因子叶日龄不同而存在很大的差异。不同日龄的子叶中,以3d龄子叶提取的DNA质量好。子叶6d龄时,提取的DNA质量次于3d龄,少量DNA开始出现降解,以后随着子叶日龄的增加,DNA降解加重。另外,与以真叶为材料提取的DNA样品相比较,子叶DNA样品中的蛋白质等杂质含量高,应增加氯仿抽提纯化次数。  相似文献   

Further suppression of<Emphasis Type="Italic">Botrytis cinerea</Emphasis> disease in cucumber seedlings by chitosan-copper complex as compared with chitosan alone     

N. Ben-Shalom  E. Fallik 《Phytoparasitica》2003,31(1):99-102

Chitosan-copper complex compared with chitosan alone enhanced suppression ofBotrytis cinerea rot development on four-true-leaf cucumber seedlings in controlled growth chambers. This paper constitutes the first report of such enhancement. The optimal concentrations for the most effective suppression ofBotrytis development were 0.2 gl ⁻¹ chitosan and 1.6 mmole copper. After 12 days’ incubation, marked and significantly better disease suppression was obtained with chitosan-copper complex (75% suppression) than with chitosan alone. The chitosan-copper complex could be a very promising decay control agent for use in both conventional and organic agriculture. http://www.phytoparasitica.org posting Dec. 18, 2002.  相似文献   

黄瓜单体异附加系的筛选与观察   总被引：9，自引：0，他引：9  

陈劲枫  罗向东  钱春桃  曹清河 《园艺学报》2003,30(6):725-727

 将甜瓜属异源三倍体与栽培黄瓜‘北京截头’杂交，经胚胎拯救获得了两个2n=15 (14C+1H)的单体异附加系，编号分别为02—17和02—39。02一l7植株叶形为戟形，02—39为深陷的掌状形，并且果刺均为白色，不同于异源三倍体的掌状心脏形和黑刺，果形比异源三倍体黄瓜长而更接近普通黄瓜。细胞学研究发现，单体异附加系在终变期和中期I染色体构型主要是由7个二价体和1个单价体构成，出现三价体的频率为4％ ～5％ ，中期Ⅱ不均等分裂形成不同的两极，一极染色体为8条，另一极为7条。RAPD分析证明它们分刖附加了野生种Cucum／s hystrix的染色体。  相似文献   

Transgenic cucumber expressing an endogenous class III chitinase gene has reduced symptoms from Botrytis cinerea     

Kyutaro Kishimoto  Yoko Nishizawa  Yutaka Tabei  Masami Nakajima  Tadaaki Hibi  Katsumi Akutsu 《Journal of General Plant Pathology》2004,70(6):314-320

A class III chitinase gene (CHI2) is induced in cucumber plants (Cucumis sativa L.) in response to infection by pathogenic microorganisms. Infection of Botrytis cinerea, causal agent of gray mold disease on cucumber, also induces CHI2 expression. To investigate whether CHI2 is involved in resistance to gray mold disease, transgenic cucumber plants were produced to overexpress the CHI2 gene. One line was analyzed in detail in terms of disease resistance. The transgenic cucumber plant (CC2) constitutively expressed CHI2 and reduced the symptoms of B. cinerea for 4 days after inoculation compared with nontransgenic plants. However, this inhibitory effect was not absolute, and CC2 eventually developed serious disease symptoms. Chitinase activity of the crude extract from CC2 leaves was higher than that from nontransgenic plants. A high-molecular-weight fraction containing CHI2 from CC2 leaves had fungistatic activity against B. cinerea. Interestingly, the low-molecular-weight fraction from CC2 leaves with CHI2 removed also had fungistatic activity against B. cinerea. Not only the introduced chitinase activity but also the endogenous defense reactions activated by overexpression of CHI2 may be involved in the enhanced gray mold disease resistance in CC2.  相似文献   

两个新的黄瓜SnRK2基因的鉴定及特征分析     

曹齐卫  周建贵  刘明毓  王永强  杨桂兰  孙小镭  李利斌 《山东农业科学》2015,(7)

植物SnRK2基因在非生物逆境应答中具有重要功能,但有关黄瓜SnRK2基因的研究尚缺乏报道。笔者通过对黄瓜基因组的重新挖掘,发现了2个新的SnRK2基因,分别与拟南芥的SnRK2.4和SnRK2.10同源。本试验对2个新的黄瓜SnRK2基因的结构特征及顺式调控元件进行了全面分析,为进一步研究其在黄瓜逆境应答中的功能奠定了基础。  相似文献