青霉素V钾滴丸的研制     

肖国君  陈力  易木林  陈波 《中国兽药杂志》2003,37(6):17-19

为减少青霉素V钾的水解并提高其疗效，用聚乙二醇400和聚乙二醇6000为基质制备青霉素V钾滴丸。体外抑菌试验表明，青霉素v钾滴丸抑菌作用略强于人药片剂。  相似文献   

中国野生种毛葡萄光合特性的研究   总被引：17，自引：1，他引：16  

朱林  温秀云  李文武 《园艺学报》1994,(1)

毛葡萄（ＶｉｔｉｓｑｕｉｎｑｕａｎｇｕｌａｒｉｓＲｅｈｄ）的净光合速率日变化值明显高于山葡萄、宝石、白玉霓，且无栽培品种光合作用的“午休”现象。毛葡萄的净光合速率与其它野生种葡萄和栽培品种比较也表现出较高水平。在进行ＣＯ２饱和点和补偿点测定时，毛葡萄也较栽培品种白玉霓有较强的光合作用，因此，毛葡萄是葡萄高光合效率育种的优良野生种质资源。  相似文献   

The intravenous bile Acid loading test in the goat: determination of a procedural protocol and reference values     

Hagemoser WA 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1989,18(1):17-20

An intravenous bile acid loading test was performed on 16 goats using various dosages and sampling protocols. A recently developed enzymatic bile acid assay provided an economical and technologically simple method for bile acid analysis. Intravenous injection of bile salts into goats was well tolerated. Clearance times were rapid. A protocol for performance of the test was suggested and a practical reference range for T 1/2 clearance of 4+/-1 minute was determined. Further investigations of this test in the assessment of liver function in the ruminant appear warranted.  相似文献   

Immunosurveillance and tumorigenesis in BALB/c mice--1. Early reactive state in Peyer's patches.     

V A Toma  F P Retief 《Comparative immunology, microbiology and infectious diseases》1979,2(1):43-53

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The in situ cellular immune response in BALB/c mice Peyer's patches after stimulations with E. coli endotoxin (ECE).     

V A Toma  F P Retief 《Comparative immunology, microbiology and infectious diseases》1979,2(1):31-41

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Contribution to the taxonomy and pathogenicity of fungicolous Verticillium species. II. Pathogenicity     

Dr. A. van Zaayen  Dr. W. Gams 《European journal of plant pathology / European Foundation for Plant Pathology》1982,88(4):143-154

In recent years in the Netherlands a second mushroom species,Agaricus bitorquis, which prefers higher temperatures thanA. bisporus and is less susceptible to certain diseases, is often commercially grown.Verticillium fungicola var.fungicola, the causal agent of dry bubble, is responsible for considerable damage in crops ofA. bisporus. InA. bitorquis, however, dry bubble has hardly been noticed, but brown spots due toV. fungicola var.aleophilum resulted in inferior mushroom quality. The latter variety also caused brown spots ina. bisporus, but to a minor degree. In variety Les Miz 60 ofA. bisporus, however, it also induced fruit-body deformation in a way different from dry bubble. Verticillium psalliotae, isolated fromA. bitorquis in England, induced more confluent brown spots inA. bitorquis. In the netherlands, where moreA. bitorquis is grown than in other countries,V. psalliotae has not yet been encountered in crops ofA. bitorquis. V. psalliotae, which has a high temperature optimum for mycelial growth, likeV. fungicola var.aleophilum andA. bitorquis, did not infectA. bisporus in our trials.Artificial infection ofA. bisporus orA. bitorquis could not be accomplished with the following related and/or fungicolous fungi:Verticillium lamellicola, V. fungicola var.flavidum, V. biguttatum, Nectriopsis tubariicola, Acremonium crotocinigenum andAphanocladium album.Samenvatting Vooral in Nederland wordt sinds een aantal jaren naastAgaricus bisporus ook de warmteminnende champignonsoortAgaricus bitorquis geteeld, die minder vatbaar is voor bepaalde ziekten. TerwijlVerticillium fungicola varfungicola in de teelt vanA. bisporus droge mollen en daardoor veel schade veroorzaakt, komen in de teelt vanA. bitorquis geen droge mollen voor maar wel bruine vlekken, die tot kwaliteitsverlies en dus schade leiden. De vlekken bleken veroorzaakt te worden doorV. fungicola var.aleophilum. Deze schimmel veroorzaakte ook inA. bisporus bruine vlekken, hoewel in minder ernstige mate, maar in het ras Les Miz 60 vanA. bisporus bovendien misvormde champignons, die wel op droge mollen leken, maar daaraan niet gelijk waren.OokV. psalliotae, in Engeland geïsoleerd vanA. bitorquis met vlekken, veroorzaakte wat meer samenvloeiende, bruine vlekken inA. bitorquis. In Nederland, waar meerA. bitorquis geteeld wordt dan in andere landen, isV. psalliotae nog niet aangetroffen in teelten vanA. bitorquis. InA. bisporus kon geen kunstmatige infectie worden verkregen metV. psalliotae, die net alsV. fungicola var.aleophilum enA. bitorquis warmteminnend genoemd zou kunnen worden.Met de volgendeVerticillium-achtige of van paddestoelen geïsoleerde schimmels kon evenmin op kunstmatige wijze een infectie worden opgeroepen inA. bisporus ofA. bitorquis: Verticillium lamellicola, V. fungicola var.flavidum, V. biguttatum, Nectriopsis tubariicola, Acremonium crotocinigenum enAphanocladium album.  相似文献   

鸭梨酶促褐变的生化机制及底物鉴定   总被引：29，自引：0，他引：29  

吴耕西  周宏伟 《园艺学报》1992,19(3):198-202

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低氧胁迫下黄瓜幼苗根系多胺代谢的变化   总被引：23，自引：2，他引：23  

汪天  王素平  郭世荣  高洪波 《园艺学报》2005,32(3):433-437

 利用外源Spd、PAO 抑制剂研究了黄瓜幼苗根系在低氧水培时的生长状况以及内源PAs、H₂O₂ 含量和PAO活性的动态变化。结果表明, 外源PAs对低氧胁迫下黄瓜幼苗根系的生长有促进作用;低氧处理后3种PAs含量都明显上升, 外源Spd和PAO抑制剂增加了根系中的PAs含量, 降低了根系中H₂O₂ 含量, 减缓了低氧伤害; PAs为低氧胁迫的应激产物, PAO为适应酶类, 其活性随PAs含量的变化而发生变化。  相似文献   

套袋黄冠梨黑点病与钙素营养和果实衰老的关系   总被引：24，自引：0，他引：24  

王文辉  李振茹  王志华  佟伟  亓丽萍 《果树学报》2005,22(6):658-661

为探明套袋黄冠梨果实黑点病发病原因,从采后生理、矿质营养水平、酚类物质酶促褐变和果实衰老等方面对套袋黄冠梨果实黑点病进行了研究。结果表明:套袋果呼吸强度显著高于不套袋果;套袋果Ca含量仅为不套袋果的49%-63%,果皮钙素含量未套袋果>套袋健康果>套袋病果,套袋病果与不套袋果果皮N/Ca比值分别为9.8和6.1,K/Ca比值分别为17.6和8.8;果皮和果心酚类物质含量及多酚氧化酶活性(PPO)呈套袋病果>套袋健康果>未套袋果的趋势;套袋病果果皮丙二醛(MDA)含量及电导率显著高于未套袋果;果皮钙素含量与其PPO活性、多酚及丙二醛含量、电导率呈高度负相关。初步分析黄冠梨果实黑点病病因为缺钙性生理衰老。  相似文献   

Induction of an antioxidant enzyme system and other oxidative stress markers associated with compatible and incompatible interactions between chickpea (Cicer arietinum L.) and Fusarium oxysporum f. sp.ciceris   总被引：1，自引：0，他引：1  

Carmen García-Limones  Ana Hervs  Juan A. Navas-Corts  Rafael M. Jimnez-Díaz  Manuel Tena 《Physiological and Molecular Plant Pathology》2002,61(6):325

To ascertain if active oxygen species play a role in fusarium wilt of chickpea caused by Fusarium oxysporum f. sp. ciceris, the degree of lipid peroxidation (malondialdehyde formation) and the activity levels of diamine oxidase (DAO), an apoplastic H₂O₂-forming oxidase, and several antioxidant enzymes, namely ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), guaiacol-dependent peroxidase (GPX) and superoxide dismutase (SOD), were determined spectrophotometrically in roots and stems of ‘WR315’ (resistant) and ‘JG62’ (susceptible) chickpea cultivars inoculated with the highly virulent race 5 of the pathogen. Moreover, APX, CAT, GPX and SOD were also analysed in roots and stems by gel electrophoresis and activity staining; and the protein levels of APX and SOD in roots were determined by Western blotting. In roots, infection by the pathogen increased lipid peroxidation and CAT and SOD activities, although such responses occurred earlier in the incompatible compared with the compatible interactions. APX, GPX and GR activities were also increased in infected roots, but only in the compatible interaction. In stems, infection by the pathogen increased lipid peroxidation and APX, CAT, SOD and GPX activities only in the compatible interaction, and DAO activity only in the incompatible one. In general, electrophoregrams agreed with the activity levels determined spectrophotometrically and did not reveal any differences in isoenzyme patterns between cultivars or between infected and non-infected plants. Further, Western blots revealed an increase in the root protein levels of APX in the compatible interaction and in those of SOD in both compatible and incompatible interactions. In conclusion, whereas enhanced DAO activity in stems, and earlier increases in lipid peroxidation and CAT and SOD activities in roots, can be associated with resistance to fusarium wilt in chickpea, the induction of the latter three parameters in roots and stems along with that of APX, GR (only in roots) and GPX (only in stems) activities are rather more associated with the establishment of the compatible interaction.  相似文献