Genotype evaluation,muscle histopathology and ultrastructure in a Duchenne muscular dystrophy animal model     

LI Zhong  ZHANG Cheng  CHEN Guo-jun  LIU Xiao-rong 《园艺学报》2004,20(3):311-316

AIM: To evaluate the genotype , muscle histopathology and ultrastructure in dko mice. METHODS: Dystrophin/Utrophin-deficient double knockouts (dko) mice were obtained from university of Oxford, UK. Genotype of filial generation of heterozygote was evaluated by PCR-SSP. HE staining and fluorescent immunohistochemistry by SABC-Cy3 were used to detect striated muscle of dko mouse, and the muscle ultrastructure was observed by transmission electron microscope(TEM). RESULTS: In 112 filial generation mice, there were 28 mdx (25.0%), 26 dko (23.2%) and 58 heterozygote (51.8%), which coincided with the law of Mendelian inheritance. HE staining showed that the myocytes were not very uniform, there were phenomenon of round outline, centrally nucleated fibers, widening interspace, inflammatory cell infiltration and connective tissue proliferation in dko mice. There were no any immunofluorescent expression of dystrophin and utrophin in sarcolemma in dko mice. TEM showed sarcolemma breakage, separation and edema, and loose myofibril texture, inflammatory cell infiltration and connective tissue proliferation in dko mice. CONCLUSION: PCR-SSP is a very quick and accurate way for genotype evaluation of filial generation. The pathophysiology of dko mouse was very similar to Duchenne muscular dystrophy (DMD), and dko mouse is an ideal animal model for study of DMD clinical therapy.  相似文献   

靠接换砧矫治枳砧甜橙缺铁黄化研究     

韩为璨  张清华  李银国  王成秋  甘永康  王锡富  张方学  舒洪永 《中国南方果树》1993,(2)

靠接红桔、酸桔和枸头橙砧,矫治枳砧甜橙缺铁黄化有明显效果。其中,以枸头橙效果最好,以下依次为酸桔和红桔,靠接砧苗后树势逐年恢复,产量成倍增加,靠接红桔、酸桔和枸头橙分别比对照增产3.3、1.4和1.5倍;对提高果实含糖量、果汁率和降低含酸量亦有良好效果。  相似文献   

确定插齿刀廓形的方法及精度分析   总被引：1，自引：0，他引：1  

赵敬云  徐珂 《拖拉机与农用运输车》2006,33(6):90-91,96

论述了由插齿刀前锥面和螺旋面方程确定的母面廓形,提出了保证插齿刀原始廓形角的3个条件,由此推出了计算廓形角的3种方法,得出了廓形角及基圆半径的计算公式,然后对3种方法的误差进行分析,计算表明第3种方法误差较小,是一种较好方法。  相似文献   

A review of models of landscape change   总被引：23，自引：1，他引：22  

William L. Baker 《Landscape Ecology》1989,2(2):111-133

Models of landscape change may serve a variety of purposes, from exploring the interaction of natural processes to evaluating proposed management treatments. These models can be categorized as either whole landscape models, distributional landscape models, or spatial landscape models, depending on the amount of detail included in the models. Distributional models, while widely used, exclude spatial detail important for most landscape ecological research. Spatial models require substantial data, now more readily available, via remote sensing, and more easily manipulated, in geographical information systems. In spite of these technical advances, spatial modelling is poorly developed, largely because landscape change itself is poorly understood.To facilitate further development of landscape models I suggest (1) empirical multivariate studies of landscape change, (2) modelling of individual landscape processes, (3) explicit study of the effect of model scale on model behavior, and (4) scaling-up results of studies, on smaller land areas, that have landscape relevance.  相似文献   

基于纵向传导的蔬菜批零价格关系研究——以北京市果类蔬菜为例     

李靓  穆月英 《中国蔬菜》2017,1(6):62-69

从蔬菜产业的纵向看,批发和零售是两个重要环节,蔬菜批发价格和零售价格的走势以及它们之间的关系是各界关注的焦点。本文以北京市场为例,以番茄和黄瓜两种果类蔬菜的按日批零价格为对象,运用有限分布滞后模型和误差修正模型进行实证分析,研究蔬菜批零价格间的传导关系,并在此基础上揭示蔬菜批发价格在蔬菜价格形成中的作用。研究发现:蔬菜批零价格走势基本一致,相关系数很高;批零价格间存在批发价格向零售价格单向传导的因果关系;批零价格的传导是顺畅的,但不同种蔬菜传导的时间效应有所不同;批发价格在蔬菜价格形成中占据主导作用,当蔬菜价格受到冲击时,若零售价格过高则其会向着批发价格下调,若批发价格过高则零售价格会随之走高。  相似文献   

舒城县油茶低产林类型与综合改造技术模式   总被引：1，自引：0，他引：1  

黄贤圣 《安徽林业》2011,(5)

舒城县是安徽省油茶重点发展县,油茶产业开发走在全省的前列。本文阐述了舒城县现有油茶低产林类型、改造模式及其技术措施,对分类实施油茶低改具有一定现实意义和指导意义。  相似文献   

造林施工偏差对幼龄林生长及抚育的影响     

薛顺英  张路凤  王文德 《青海农林科技》2011,(3):60-61

本文通过开展中幼龄林抚育作业设计，调查不同林分因子，得出造林施工偏差对幼龄林林分结构、林木冠形、根系生长、胸径生长及抚育工作有着显著的影响。  相似文献   

一体化微操作器误差分析与建模     

纪华伟  杨世锡  吴昭同 《农业机械学报》2007,38(3):136-140

为评价各种误差对微操作器精度的影响，以一体化超精密微操作器为研究对象，分析了加工误差、机构原理误差、测量误差、环境带来的误差以及驱动误差等造成微操作器精度降低的因素，建立了加工误差的偏微分方程解析模型，并用有限元仿真验证了加工误差模型的准确性。在详细分析误差源的基础上，提出了提高微操作器精度的措施。  相似文献   

基于腕力传感器的机器人操作臂末端位置误差补偿   总被引：2，自引：0，他引：2  

干方建  刘正士 《农业机械学报》2001,32(6):103-105

针对机器人操作臂夹持工具与环境作用时基于传感器坐标系内的微分运动分析 ,推导出基于工具坐标系的定位误差及基于基坐标系的定位误差补偿 ,并以 PU MA5 60型机器人为例计算了定位误差及其误差补偿  相似文献   

红碎茶CTC齿辊数控加工及误差统计研究   总被引：1，自引：0，他引：1  

尹志强  刘光复  史国川  朱保国  薛云林 《农业机械学报》2001,32(4):57-59,63

介绍了自行研制的红碎茶CTC齿辊微机数控加工车床的总体方案，进给系统结构设计以及微机数控硬件系统主要构成、软件功能和加工子程序。利用数理统计学方法，对齿辊3mm节距误差进行了分析研究，结果表明采用微机数控加工齿辊，节距精度得到明显提高。该机同时也保留了普通车床基本功能。  相似文献