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Yuichiro Sato Kinjiro Morimoto Takanori Kubo Takemasa Sakaguchi Akira Nishizono Makoto Hirayama Kanji Hori 《Marine drugs》2015,13(6):3454-3465
Lectin sensitivity of the recent pandemic influenza A virus (H1N1-2009) was screened for 12 lectins with various carbohydrate specificity by a neutral red dye uptake assay with MDCK cells. Among them, a high mannose (HM)-binding anti-HIV lectin, ESA-2 from the red alga Eucheuma serra, showed the highest inhibition against infection with an EC50 of 12.4 nM. Moreover, ESA-2 exhibited a wide range of antiviral spectrum against various influenza strains with EC50s of pico molar to low nanomolar levels. Besides ESA-2, HM-binding plant lectin ConA, fucose-binding lectins such as fungal AOL from Aspergillus oryzae and AAL from Aleuria aurantia were active against H1N1-2009, but the potency of inhibition was of less magnitude compared with ESA-2. Direct interaction between ESA-2 and a viral envelope glycoprotein, hemagglutinin (HA), was demonstrated by ELISA assay. This interaction was effectively suppressed by glycoproteins bearing HM-glycans, indicating that ESA-2 binds to the HA of influenza virus through HM-glycans. Upon treatment with ESA-2, no viral antigens were detected in the host cells, indicating that ESA-2 inhibited the initial steps of virus entry into the cells. ESA-2 would thus be useful as a novel microbicide to prevent penetration of viruses such as HIV and influenza viruses to the host cells. 相似文献
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为了给人参果病毒检验及抗病性研究提供支持,对人参果采用ELISA和RT-PCR两种检测方法对8份田间材料中的马铃薯M病毒(PVM)、番茄花叶病毒(ToMV)和烟草花叶病毒(TMV)进行检测,对比筛选病毒检测方法。结果表明,ELISA法检测PVM的阳性检出率为87.5%;ToMV为37.5%,疑似率为12.5%;TMV为37.5%。通过RT-PCR检测体系,从人参果样品中分别扩增出与试验设计大小相符的特异条带,PVM阳性检出率为100%,ToMV为 50.0%,TMV为37.5%,检测灵敏性和准确性更高,检测结果符合率在87.5%以上。对扩增阳性产物进行凝胶回收测序,确定为目标条带。 相似文献
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人参果组织培养技术研究 总被引：3，自引：0，他引：3
以人参果的幼嫩茎段和叶片作外植体，在MS基本培养基附加不同浓度6-BA IAA,6-BA NAA 和 KT 2，4 D 组成的固体培养基中进行培养．结果显示：MS 6-BA 0．07 mg／L IAA 0.03mg／L，MS 6-BA 0.70mg／L IAA 0.03mg／L，MS 6-BA 0.70mg／L IAA O.05mg/L，MS 6-BA 1.00mg/L IAA 0.05mg/L 4种培养基中的材料愈伤组织长势比较好，分化明显、经继代培养，丛生芽大量形成，丛生苗根系发达、其他培养基则停留在愈伤组织阶段或只长出极少数短的根. 相似文献
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Chandrappa Gangaiah Amjad A. Ahmad Hue V. Nguyen 《Communications in Soil Science and Plant Analysis》2016,47(17):2046-2052
Nutritional status of vegetable crops is often monitored by analysis of dried plant tissues, which is costly and often time consuming. Two greenhouse trials were conducted, at the University of Hawaii at Manoa, Magoon facilities, to evaluate the portable cardy ion meter (CIM) in determining potassium (K+) status in fresh petiole sap of pak choi as compared with standard laboratory methods. In the first greenhouse trial, three algae species (Gracilaria salicornia, Kappaphycus alvarezii, and Eucheuma denticulatum) were used to apply five rates of K+ (0, 84, 168,252, and 336 kg.ha?1). The pak choi was directly seeded into 4 L pots and was grown in peat moss. In the second greenhouse trial, K+ was provided through Eucheuma denticulatum and potassium nitrate (KNO3) at five rates (0,112, 168, 224, 280, and 336 kg.ha?1) in peat moss and soil media. At harvest, K+ concentrations in fresh petiole sap were analyzed immediately with CIM and the dried samples were analyzed by inductively coupled plasma spectroscopy (ICP) measurement. The results showed increase in leaf K+ content at higher rates and the maximum concentration of leaf K+ at 4500–5300 mg/L for sap and 8–9% for tissue was obtained when K+ was provided between 224 and 284 kg.ha?1. There was a close correlation between the CIM readings and the ICP method (r = 0.8048 and 0.8314) from the first and second GH results, respectively. The results suggest that the CIM could be used for the rapid monitoring of the relative K+ status of plants. The data further suggested 4500–5000 mg K/L for fresh petiole sap and 7.5% K+ in tissue as critical levels for K+ concentration in pak choi. 相似文献