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1.
采用聚丙烯酰胺凝胶电泳分析了来至水稻和马唐寄主的23个致病性不同的稻瘟菌菌株的可溶性蛋白、酯酶和酸性磷酸酯酶的图谱。结果表明，二类寄主菌株的可溶蛋白和酯酶图谱有明显不同，而酸性磷酸酯酶图谱的差异，不显著。同一寄主内的菌株，彼此在电泳图型上非常相似，只是在少数带的染色强弱上有一定差异．但未发现这些话带的变比同病菌致病性直接相关的依据。 相似文献
2.
《African Journal of Range and Forage Science》2013,30(2):131-145
This study assessed the importance of spatial and temporal variation in plant quality and quantity for determining sustainable stocking rates in game, commercial and communal ranches in semi-arid savanna of the Northern Cape Province, South Africa, in wet and dry seasons over a two-year period. We focussed on variation in plant biomass, phosphorus (P), crude protein (CP) concentrations and dry matter digestibility as parameters most likely to affect sustainable stocking rates. Habitat type had greater effects on plant quality, plant biomass and species composition than management type. The commercially-managed area had the highest tree density in the rocky habitat and lower plant quality than other management types. All of these features indicate that land degradation is occurring on commercial ranches in spite of rotational grazing and lower stocking density than on communal ranches. We recommend that commercial ranchers should introduce a greater variety of stock and/or game to reduce selective grazing of certain plant species. Quality measures (CP and P) gave more conservative predictions of stocking density than biomass. In this region of the Northern Cape, seasonally-inundated pan habitats are particularly valuable in spite of low-standing crop because they have the highest year-round quality. Contrastingly, ranchers should only lightly stock open savanna habitats, in spite of high standing biomass, because they have low vegetation quality and may be particularly susceptible to degradation and invasion by poisonous and unpalatable plants. 相似文献
3.
《African Journal of Range and Forage Science》2013,30(3):243-246
Wall barley (Hodreum murinum L.) is an annual cool-season grass species that grows in areas with a Mediterranean climate. It has potential as a forage source in Jordan. The objective was to determine seasonal chemical composition of wall barley grown under sub-humid Mediterranean conditions. A field trial was conducted during 2000 and 2001 growing seasons at Samta (32°23'N, 35°50'E) in the Ajloun Mountains, Jordan. Data were collected on 28 February, 15 March, 30 March, 15 April and 15 May. The lowest fibre content of leaves was on 28 February and 15 March, with a range of 19.5% to 23.3%. Fibre content of leaves (73.3%) and stems (79.9%) peaked when the plants were mature (15 May). In contrast to fibre, the protein content decreased gradually with age. Protein content of the leaves on 28 February was 25%, after which it declined gradually and reached 2.9% at maturity. The calcium content of the leaves was higher than that of the stems and was sufficient to meet ewes' maintenance requirements. The Ca content of leaves during the period from 28 February to 30 March was 1.2% to 1.1%, whereas, the content declined with age and reached the lowest value (0.01%) at maturity. The phosphorus content did not show significant variation. It is concluded that age and environmental conditions may affect the nutritive value of wall barley. 相似文献
4.
雏鸭实用饲粮锌的适宜添加量研究 总被引：3，自引：0，他引：3
分别喂给1～35日龄建川鸭二种蛋白水平和不同含锌量的植物性饲粮,测定了不同锌水平饲粮对雏鸭生长和某些组织锌含量的影响,初步探讨了蛋白质水平与锌吸收、利用的关系。试验结果表明,雏鸭(1～35日龄)风干饲粮锌的适宜添加量为54ppm(总锌为71ppm),饲粮适宜蛋白质水平能提高锌的利用率。 相似文献
5.
营养水平对猪胎儿器官发育和初生仔猪血液生化指标的影响 总被引：2，自引：0，他引：2
本实验选用初配体重分别为５６．８、７０．１６、８４．２８ｋｇ的雅南小母猪各６头，在怀孕０－８０天、８１天至分娩分别定量采食１．１３、１．６１、２．０９公斤／头·日和１．５７、２．２４、２．９１公斤／头·日来研究孕期营养水平对初生仔猪器官重和血液生化指标的影响。研究结果表明：孕期母体营养水平对初生仔猪心、脑重没有显著影响（Ｐ＞０．０５），对肝、肾、脾有显著影响（Ｐ＜０．０５），胎儿肾、脾生长发育对母体营养水平变化最敏感，肝中等，脑、心不过感；初生仔猪血清总蛋白、清蛋白含量受母体营养水平影响（Ｐ＜０．０５）；葡萄糖则不受影响（Ｐ＞０．０５）；初生仔猪血清总蛋白、清蛋白含量与母体蛋白摄入量呈二次曲线相关，其方程分别为：Ｙ＝－１５１４．７４５＋２４５２．４７７Ｘ１－８３８．６６３Ｘ１２（Ｐ＜０．００１）Ｙ＝－１２８８９．４８＋３０４３３．７７Ｘ２－１７５９０．９５Ｘ２２（Ｐ＜０．０５）（Ｙ为母体蛋白摄入量：克／头·日；Ｘ１、Ｘ２分别为初生仔猪血清总蛋白、清蛋白含量：ｍｇ／１００ｍｌ）；初生仔猪猪血清清蛋白含量可以作为确定孕期母体蛋白需要量的标识；初生长雅仔猪血清葡萄糖、清蛋白、总蛋白含量分别为７１．７２ｍｇ／１００? 相似文献
6.
小麦穗腐镰刀菌的蛋白质和酸性磷酸酯酶凝胶电泳分析及其分类意义 总被引：1，自引：0，他引：1
对引致小麦赤霉病穗腐镰刀菌(Fusarium)的10个种或变种及30个不同地理来源的禾谷镰刀菌株的菌体可溶性蛋白质和酸性磷酸酯酶的聚丙烯酰胺凝胶电泳分析结果表明,蛋白质电泳图谱在大多数穗腐镰刀菌种或变种间存在着很大相似性;在禾谷镰刀菌种内,30个不同地理来源菌株间的电泳图型也基本相同,因此很难将这一生化指标用于穗腐镰刀菌的分类。十个穗腐镰刀菌种或变种间的酸性磷酸酯酶同工酶均为二条带,由于带数少,提供的分类信息少、加之彼此间有很大相似性,因此该酶在分类上没有意义。 相似文献
7.
采用电泳法对滨湖水牛血红蛋白、血清运铁蛋白、淀粉酶、白蛋白的多态性进行了检测。结果表明，血红蛋白多态型表现为ＡＡ、ＡＢ、ＢＢ三种类型，其频率分别为０．０１２、０．０５８、０．９３０，并且发现一头三条泳带的稀有变异体，基因ＨｂＡ、ＨｂＢ频率分别为０．０４１、０．９５９。运铁蛋白多态型表现为ＡＡ、ＡＤ、ＤＤ、ＡＥ、ＤＥ五种类型，频率为０．０５８、０．３５６、０．５１７、０．０１２、０．０５８，基因ＴｆＡ、ＴｆＤ、ＴｆＥ的频率为０．２４１４、０．７２４１、０．０３４５。淀粉酶多态型表现为ＡＡ、ＡＢ、ＡＣ、ＢＣ四种类型，频率为０．１２８２、０．１０２６、０．７１７９、０．０５１３，基因ＡｍｙＩＡ、ＡｍｙＩＢ、ＡｍｙＩＣ频率为０．５３８５、０．０７８９、０．３８４６。白蛋白多态型表现为ＡＡ、Ａｘ、Ｂｘ、ｘｘ四种类型，频率为０．０８０４、０．２９８８、０．０２３０、０．５９７７，基因ＡｌｂＡ、Ａｌｂｘ、ＡｌｂＢ频率０．２３００、０．７５８６、０．０１１５。对滨湖水牛遗传变异性研究表明其杂合度较低为０．２５７８。 相似文献
8.
本研究的目的是建立一种能简便、快速、准确鉴定水稻种子上寄藏的三类黄色细菌的方法。通过对三类菌株的菌体蛋白质和DNA限制性片段的电泳分析结果表明,三类细菌的蛋白质和DNA限制性片段电泳图谱明显不同,而在同一类群内不同菌株间的电泳图型则基本相同,因此能应用蛋白质和DNA限制性片段电泳分析于这些类群细菌的分类鉴定和亲缘关系分析,特别是菌体蛋白质是电泳分析具有简便、快速等优点。 相似文献
9.
Abstract Embryogenic callus was obtained from immature regions of the leaf of Digitaria eriantha when grown in the dark on a MS medium supplemented with 1,0 or 2,0mg/dm3, 2,4‐D and 3–9 % sucrose. Plantlet regeneration occurred when the calli were transferred to MS medium containing 0,1 mg/dm3, 2,4‐D and 7–8 % sucrose, and placed under 16 hour photoperiod. The regenerated plantlets were successfully planted out into a mixture of sand and potting soil. 相似文献