Arthrographis kalrae soluble antigens present hemolytic and cytotoxic activities     

《Comparative immunology, microbiology and infectious diseases》2014,37(5-6):305-311

Arthrographis kalrae is a dimorphic, cosmopolitan and neurotropic fungus that has been described as a rare human pathogen. This study investigated the hemolytic and cytotoxic activities of A. kalrae cell-free antigens (CFAs). Total CFAs and their Sephadex chromatography fractions were tested on mouse erythrocytes for hemolysis and on the P3U1 cell line for cytotoxicity. Hemolytic and cytotoxic activities were detected in distinct molecular mass (MM) fractions. Additionally, antibodies against isogenic erythrocytes sensitized with CFAs (anti-E-CFAs) inhibited hemolysis but not cytotoxicity. Hemolysis was not affected by heating, and a higher reactivity was detected in the carbohydrate-rich fractions, which decreased after reduction by periodate treatment. The pioneering nature of this work is due to the demonstration of the cytotoxic activity in A. kalrae and the suggestion that this activity may be due to molecules distinct from the hemolytic factor, with the latter potentially being a component with a high MM.  相似文献   

Interactions between arbuscular mycorrhizal fungi and fungivorous nematodes on the growth and arsenic uptake of tobacco in arsenic-contaminated soils     

《Applied soil ecology》2014

The effects of inoculation with two AM fungi (M1, Glomus caledonium; M2, Glomus spp. and Acaulospora spp.) and a fungivorous nematode Aphelenchoides sp. on growth and arsenic (As) uptake of Nicotiana tabacum L. were investigated in soils contaminated with a range of As. The reproduction of Aphelenchoides sp. was triggered by the co-inoculation of AM fungi regardless of AM fungal isolates and As levels. Stimulative effects of Aphelenchoides sp. on the development of mycorrhiza, slightly different between two AM fungi, were found particularly at the lowest As level. Irrespective of mycorrhizal inoculi, increasing soil As level decreased plant growth, but increased plant As uptake. Co-inoculation of AM fungi and Aphelenchoides sp. led plants to achieving further growth and greater As accumulation at the lowest As level. Results showed that the interactions between AM fungi and fungivorous nematodes were important in plant As tolerance and phytoextraction at low level As-polluted soil.  相似文献   

绿肥影响农田土传病害的研究进展     

魏勇  高嵩涓  曹卫东  段廷玉 《草地学报》2021,29(8):1605-1614

土传病害是制约我国农业可持续发展的因素之一。绿肥作为高效清洁的有机肥源,与主作物合理搭配,可有效降低主作物土传病害的发生和危害,是减少化学药剂使用的重要措施。因此,综述绿肥对土传病害的防控效果与机制,对于绿肥推广应用和土传病害绿色防控具有重要意义。绿肥影响作物土传病害的研究主要集中于土传真菌病害和线虫病害,且大多数研究表明,绿肥可通过增强主作物整体抗病水平,对病原菌的化感抑制作用和改善土壤理化性质,增加土壤微生物中拮抗菌多样性和丰富度等,显著降低主作物土传病害发病率和严重程度。然而,绿肥影响主作物病害的作用机制研究不够深入,绿肥-主作物搭配模式和绿肥调控主作物病害的社会经济学效益仍需探究。同时,加强绿肥-主作物病害一体化防控及绿肥与根际促生菌、生防菌等其他有机改良剂或与适当的化学防控措施之间协同作用的研究,是未来需要加强的研究方向。  相似文献   

铁路货车电能收集装置的机械结构设计     

徐旭  张航  王自超  洪海峰  周琪 《湖南农机》2013,(3):67-68,73

针对转K6型转向架,设计了一种适用于铁路货车的低成本、利用自身能量发电,满足铁路货车低功率用电需求的供电装置。此装置利用滚珠丝杠将悬挂系统的上下振动转变为发电机转子的旋转。通过分析弹簧中的空间以及货车运行过程中的振动情况,设计了滚珠丝杠、外壳等零件,并通过SolidWorks软件对装置进行了仿真。  相似文献   

覆盖对果园土壤真菌数量年变化的影响   总被引：5，自引：0，他引：5  

高美英  冀常军  王中英  祁素萍 《山西农业科学》2000,28(1):46-48

试验结果表明，在整个果树生长期内，覆盖可明显增加各土层中真菌的数量，０ ～２０ｃｍ 土层中，年平均真菌数量增加７９ ．３９％ ，２０ ～４０ｃｍ 土层中增加２８ ．２４ ％ ，４０ ～６０ｃｍ 土层中增加８５ ．０５ ％ 。无论覆盖与对照，不同土层真菌数量年变化均呈单峰曲线变化，高峰出现在８ 月份。  相似文献   

酸马奶中酵母菌部分代谢特性的研究     

魏元  李景茹  李海 《农产品加工．学刊》2011,(1):42-44,53

对锡盟地区马奶酒中分离的11株酵母菌的代谢特性进行了研究。研究内容包括:酵母菌对蛋白质利用能力及酵母菌对脂肪利用能力。采用OPA法,考查了蛋白酶的活力,通过测定酵母菌中蛋白酶对乳中酪蛋白的利用,反映酵母菌对蛋白质的代谢情况;采用平板扩散法,考查了脂肪酶的活力,通过观测透明圈直径的大小来反映酵母菌对脂肪的代谢情况。  相似文献   

聚类分析在分枝定界法中的应用     

于绍慧 《湖南农业大学学报(自然科学版)》2008,(2)

针对界约束二次规划的分枝定界法中出现的紧、松弛策略,结合聚类分析方法,给出了新的剖分边的选取原则,把球约束二次规划作为子问题,使得原问题整体最优值的上、下界能较快的达到.  相似文献   

女子沙滩排球垫球调整在比赛中的运用对比分析     

杨景淼 《四川畜牧兽医学院学报》2008,(2):118-120

通过文献资料法、录像统计法、观察分析法，以部分世界女子沙滩排球巡回赛、全国女子沙滩排球巡回赛为研究对象，对沙滩排球垫球调整技术在比赛中的运用进行研究分析。认为沙滩排球比赛受自然环境的影响和规则的限制，传球调整容易造成持球和连击，而我国沙滩排球队伍仍然更多采用传球调整技术。重视垫球调整是提高我国沙滩排球技战术水平与世界接轨的发展趋势，也是适应自然条件和适应规则的有效措施。  相似文献   

几丁质酶在植物抗真菌病害中的作用   总被引：7，自引：0，他引：7  

张福丽  王占斌  王志英 《林业科技》2006,31(3):24-27

几丁质酶对于植物抗真菌病害有重要作用,它可水解真菌细胞壁的重要组成成分———几丁质。正常情况下,植物体内几丁质酶的含量很低,如果将外源几丁质酶基因导入植物,可增强植物抗真菌病害的能力,而将几丁质酶基因与其他防卫蛋白基因一同导入植物,植物抗真菌病害的能力将会大大提高。  相似文献   

Update on Fungal Respiratory Disease in Horses     

《Veterinary Clinics of North America: Equine Practice》2015,31(1):43-62

  相似文献