Cuticular protein gene LmACP8 is involved in wing morphogenesis in the migratory locust,Locusta migratoria          下载免费PDF全文

Xiao-ming ZHAO  Jia-peng YANG  Xin GOU  Wei-min LIU  Jian-zhen ZHANG 《农业科学学报》2021,20(6):1596-1606

Cuticular proteins(CPs) are major components of the insect cuticle-associated organs such as integument and wings, although the importance of CPs for wing development and function in hemimetabolous insects remains understudied. In the present study, a wing cuticular protein LmACP8 was identified from Locusta migratoria, which belongs to the RR-2 subfamily of cuticular protein RR consensus(CPR) chitin-binding proteins. LmACP8 was mainly expressed in the wing pads and showed high expression levels before ecdysis of third-, fourth-, and fifth-instar nymphs, with its encoded protein located in the procuticle of wing pads and adult wings. Depletion of LmACP8 by RNA interference markedly reduced the amount of its protein, which consequently caused abnormal wing morphogenesis in the transition from nymph to adult of L. migratoria. We further demonstrated that the abnormal morphogenesis was caused by severe damage of the endocuticle in the wings. LmACP8 was suppressed by 20-hydroxyecdysone(20 E) in vivo, however, its expression was significantly up-regulated after knocking down the hormone receptor gene LmHR39. Thus, the LmACP8 that is negatively regulated by the LmHR39-mediated 20 E signaling pathway is involved in wing development during the nymph to adult transition.  相似文献   

漆树酶在漆酚树脂上的固定化研究   总被引：4，自引：2，他引：2  

雷福厚  郭明高 《林产化学与工业》1996,16(4):19-22

介绍了漆酚树脂吸附金属离子后对漆树酶的固定化方法，比较了Ｆｅ＾３＋和Ａｌ＾３＋、漆酚树脂和漆酚－水杨酸接枝树脂对漆树酶的固定化结果显示，漆酚－水杨酸－Ａｌ螯合树脂对漆树酶的固定化活力最高，测定了溶液ＰＨ，环境温度对固定化漆树酶活性的航固定化漆酶的重作用性。另外，测定了固定化漆树酶的米氏常数，Ｋｍ＝４．９×１０＾－３，并讨论了Ｋｍ小于天然漆树酶的米氏常数的原因。  相似文献   

甲醛捕捉剂在胶合板生产中的应用   总被引：7，自引：0，他引：7  

胡炬亮  龚颖峰  王禹胜 《木材工业》2005,19(1):35-36

普通UF树脂胶中添加甲醛捕捉剂H300或H800后,其固化时间明显延长.采用H300或H800改性的UF醛树脂胶黏剂生产胶合板,在保证胶合强度的前提下,板材的甲醛释放量达到国标E2或E1级要求.  相似文献   

改善E1级胶合板用UF胶粘剂预压性能的研究   总被引：1，自引：0，他引：1  

焦德贤  唐朝发 《吉林林业科技》2005,34(5):45-47

利用氧化淀粉在树脂合成后期、API在调胶时加入分别对脲醛树脂进行改性，探讨改性UF对胶合板预压强度的影响。采用正交实验法对比改性后胶合板的预压性能和改性后胶合板的甲醛释放量的变化。结果表明：随着API量的加大，胶合板的预压性能得以提高；而氧化淀粉改性后则随着量的加大，一定范围内胶合板的预压性能得以提高，加入量继续加大后反而下降。  相似文献   

用D72树脂固定床催化莰烯制乙酸异龙脑酯   总被引：7，自引：1，他引：6  

马文龙 《林产化学与工业》1989,9(1):18-26

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催干剂对氨基环氧树脂阴极电泳漆的影响     

邓光辉  石晓红  蓝虹云  雷福厚 《林产化学与工业》2001,21(2):62-66

以氨基环氧树脂为基体树脂，Co^2 ,Pb^2 盐为催干剂合成了阴极电泳漆，并对膜性能进行考察，结果表明，所合成的阴极电泳涂料在PH值为6－7、电泳时间2min的条件下。加入不同的催干剂，在不同的烘干温度下，得到性能不同的漆膜。经过膝膜综合性能试验，结果表明，加入钴和铅复合催化剂，在160℃左右烘干20min，可得到完全固化，硬度较高、耐潮、耐盐、耐酸碱性较好的漆膜。  相似文献   

Cypermethrin inhibits egg development in the ixodid tick, Amblyomma hebraeum     

Kevin J. FriesenW.Reuben Kaufman 《Pesticide biochemistry and physiology》2003,76(1):25-35

The pyrethroid insecticide, cypermethrin (CyM), stimulates vitellogenesis in Ornithodoros moubata (an argasid tick) by stimulating the release of the normal vitellogenesis-inducing factor (a neuropeptide) and subsequent release of the vitellogenic hormone [19]. Here we examine the effects of CyM on egg development in the ixodid tick, Amblyomma hebraeum. Ovary weight, oocyte size, and vitellin content of the ovary were measured after CyM treatment; in partially fed ticks, none of these parameters were affected significantly. However, CyM treatment caused an inhibition of ovary development, as well as reduction of both hemolymph 20-hydroxyecdysone (20E; the vitellogenic hormone in this species) and vitellogenin (Vg)-concentrations in engorged ticks. In addition, the degree of salivary gland degeneration (which is triggered by 20E) was slightly reduced in CyM-treated engorged ticks. These results indicate that CyM acts differently in Amblyomma compared to Ornithodoros. Instead of stimulating vitellogenesis, CyM inhibits egg development perhaps in part as a result of inhibiting release of 20E.  相似文献   

一种脱水洋葱制品中微生物菌落总数的检测方法     

刘敏  李国鹏  张文征 《安徽农业科学》2015,(29)

建立一种准确检测脱水洋葱制品中菌落总数的方法.利用离心法对出口脱水洋葱制品中沙门氏菌进行预富集,以提高国标检测方法的致病菌检出率,弥补国标方法的不足,并对该方法的准确度进行评价.试验结果表明,样液中菌体经过高速离心后,99％以上的菌体可以从样液分离.与标准方法比对,高速离心法很好地解决了脱水洋葱粉样液中的抑菌成分的干扰,结果可靠准确,基本满足国标中食品安全标准要求,具有一定的实际推广意义.  相似文献   

6种大孔树脂对复方免疫增强剂多糖的吸附解吸性能     

李赞阳  李炳奇  刘红  龚晓武  谷新利 《西北农业学报》2011,20(12):16-18

筛选出对中药复方免疫增强剂多糖具有较好的吸附解吸效果的大孔树脂。通过静态吸附及解吸试验、动态吸附及解吸试验，利用苯酚 硫酸法跟踪检测多糖质量浓度，以吸附率与解吸率为评价指标，初步研究AB 8、LSA 5B、DM 18、CAD 40、LX 1、D941型大孔树脂对中药复方免疫增强剂中多糖的吸附及解吸性能。结果表明，在6种大孔树脂中， AB 8对中药复方免疫增强剂多糖的动态吸附率和解吸率最高，分别为54%和90%。说明AB 8型大孔树脂可以作为分离纯化多糖的材料。  相似文献   

不同极性色谱柱在滇红香气成分分析中的对比研究     

乔阳  杜丽平  肖冬光 《茶叶科学》2016,(1):38-44

采用顶空固相微萃取(HS-SPME)对滇红的香气成分进行富集,分别使用两种不同极性的色谱柱进行色谱分离,气质联用(GC-MS)检测分析,比较了非极性色谱柱HP-5MS和极性色谱柱CP-Wax对红茶香气成分分离效果的差异。结果表明,两种不同极性色谱柱对红茶香气成分的分离效果均表现良好,但分析结果存在一定的差异。因此在对红茶中不同的香气成分进行研究分析时,应根据分析目标的不同选择合适的色谱柱。  相似文献