鸡腿蘑菌丝体的同工酶研究   总被引：7，自引：1，他引：7  

邓功成  刘朝贵 《中国食用菌》2003,22(6):42-44

用8种不同培养基配方，培养鸡腿蘑菌丝体，其同工酶分析结果表明，鸡腿蘑菌丝体酯酶(EST)同工酶谱差异明显，过氧化物酶(POD)同工酶谱差异明显。  相似文献   

Allozyme Variation of Picea abies in Poland     

《Scandinavian Journal of Forest Research》2012,27(6):487-494

The aim of this study was to determine the levels and patterns of allozyme variation among 29 populations of Norway spruce [ Picea abies (L.) Karst.] from Poland. Thirteen investigated isoenzyme systems were encoded by 27 gene loci and, on average, 71% of the loci per population were polymorphic. The average and effective numbers of alleles per locus were 2.17 and 1.26, respectively, while the expected heterozygosity was 0.156. A relatively low allozyme differentiation among populations from north-eastern and southern Poland was observed ( F ST = 0.028, mean genetic distance D = 0.005). The results suggest that historical events and extensive gene flow played an important role in the distribution of the observed allozyme differentiation of Norway spruce in Poland.  相似文献   

Prognostic significance of serum alkaline phosphatase activity in canine appendicular osteosarcoma   总被引：3，自引：0，他引：3  

Garzotto CK  Berg J  Hoffmann WE  Rand WM 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2000,14(6):587-592

Sixty-one dogs with appendicular osteosarcoma were treated with amputation and chemotherapy of cisplatin and doxorubicin. Serum samples were obtained before and after treatment for determination of total alkaline phosphatase (TALP) activity as well as the activities of the constituent bone (BALP), liver (LALP), and corticosteroid-induced (CALP) isoenzymes. The relationship between alkaline phosphatase activities and survival was examined by Cox proportional hazards regression analysis and Kaplan-Meier log rank analysis. Mean activity of TALP, BALP, and LALP decreased significantly after treatment (P < .001). TALP and LALP activities before treatment were significantly correlated with survival (P = .006 and .001, respectively). The correlation between BALP activity before treatment and survival approached significance (P = .054). CALP activity and TALP, BALP, and LALP activities after treatment were not significantly correlated with survival. Dogs with normal pretreatment TALP and BALP activities survived significantly longer than dogs with increased pretreatment activities (P = .001 and .003, respectively). Median survival times for dogs with normal or increased TALP activities before treatment were 12.5 and 5.5 months, respectively; and median survival times for dogs with normal or increased BALP activities before treatment were 16.6 and 9.5 months, respectively. In the design of future clinical trials involving dogs with osteosarcoma, consideration should be given to stratifying the randomization according to alkaline phosphatase activity. In addition, alkaline phosphatase activity should be a factor considered by clinicians attempting to tailor the aggressiveness of adjuvant chemotherapy to the needs of individual patients or owners.  相似文献   

同工酶在西南地区野生狗牙根遗传多样性上的研究     

张小艾  张新全 《四川草原》2006,(4):30-32

对36份具有代表性的西南地区野生狗牙根材料采用同工酶进行遗传多样性研究,筛选出优良草坪草C1,C32,C9和部分优良育种材料,为西南地区狗牙根的开发利用及申报品种提供理论依据。  相似文献   

药用真菌菌丝体产SOD的研究初探     

黄志立  黄彦君  张丽君  刘冬  梁世中 《中国食用菌》2006,25(3):46-48

本文对9种药用及食用真菌菌丝体中SOD的同功酶进行了初步的研究．并以云芝为实验对象研究了真菌菌丝体生长与SOD活性的关系．结果表明：不同真菌来源的SOD之间同功酶组成存在较大差异：云芝菌丝体液体发酵结果显示，菌丝体的生长过程包括生长迟滞期、迅速生长期及衰亡期三个阶段．在迅速生长期后期可以获得大量富含SOD的云芝菌丝体。  相似文献   

小叶杨原生质体培养植株再生及其同工酶的变化   总被引：4，自引：2，他引：4  

王影  黄敏仁  陈道明  卫志明  孙勇如 《林业科学》1995,31(4):310-318,T001

从小叶杨（Ｐｏｐｕｌｕｓｓｉｍｏｎｉｉ）胚性悬浮细胞分离得到大量有活力的原生质体，产量高达３．８×１０７ｇ－１ＦＷ。高密度（３×１０６／ｍＬ）液体浅层培养可促进小叶杨原生质体的分裂和生长；以葡萄糖为唯一碳源的Ｋｍ８ｐ培养基，有利于原生质体的持续分裂；在培养基中添加有机氮，有助于提高原生质体的植板率。原生质体形成细胞团后，逐步降渗和分皿培养，能有效促进细胞团的持续分裂。愈伤组织在不同分化培养基上的分化频率存在着明显的差异，同工酶分析说明，分化能力强的愈伤组织，其ＴＣＡ循环和磷酸戊糖途径较为活跃，ＧＯＴ、ＥＳＴ和ＰＥＲＯＸ的活性比较高。当芽伸长到２—３ｃｍ时，从基部切下转移到无激素的１／２ＭＳ培养基上诱导生根并形成完整植株。移入盒内，在温室生长良好。  相似文献   

Research on the heredity and variation of the peruxidase isoenzyme of needles ofpinus koraiensis     

Guan Weijun 《林业研究》1993,4(1):69-72

Through the polyacrylamide gel electrophoresis analysis of thirty-two needle samples of ninty-years oldpinus koraiensis in Harbin, seven typies of zone and distribution of the zone frequency, and some objective fact of heredity and variation were determined. These informations can be used as reference materials in the research of heredity and variation ofpinus koraiensis.  相似文献   

美国红鱼养殖种群的同工酶酶谱及其生化遗传初步分析   总被引：6，自引：0，他引：6  

刘世禄  王波  高天翔  柳广东  张锡烈 《海洋水产研究》2002,23(3):10-14

利用水平淀粉凝胶电泳技术，对50尾美国红鱼肌肉及肝脏组织进行了14种同工酶的初步分析。结果表明，美国红鱼多态位点比例P＝0.292，实际平均杂合度Hp=0.057，预期杂合度He=0.108，每个位点的有效等位基因数Ae=1.108，Hardy-Weinberg遗传偏离指数D＝－0.472。  相似文献   

苹果叶片感染褐斑病过程中过氧化物酶同工酶变化的研究     

谢为龙  冷怀琼 《云南农业大学学报(自然科学版)》1990,5(3):161-165

 应用聚丙烯酰胺凝胶电泳,测定抗性不同的3个苹果品种过氧化物酶同工酶在接种褐斑病菌(Marssonina coronaria)后的变化规律。结果表明:苹果过氧化物酶同工酶在整个病程中呈类似常态曲线变化,症状出现时酶活性达最高峰,品种越感病,变化越强烈,表现在接种初期第12酶带增加早、增加量大;发病后期酶带数目比抗病品种减少更多。上述性状对病情诊断和幼苗抗性鉴定有参考价值。  相似文献   

西瓜枯萎病菌生理小种分化研究   总被引：11，自引：0，他引：11       下载免费PDF全文

王浩波  王鸣 《西北农业学报》1993,2(4):67-70

用浸根接种法对来自全国八省(市)、自治区的西瓜枯萎病菌(Fusarium oxysporium f.sp.niveum,FON)10个分离物进行了生理小种分化鉴定研究.根据这10个分离物在6个鉴别寄主上的反应,初步把它们划分为小种0,1,2,3,计4个生理小种.同时对这10个西瓜枯萎病菌分离物(FON isolates)的菌丝体进行了过氧化物酶同工酶(POD)聚丙烯酰胺凝胶电泳分析.电泳图谱的分析结果支持了浸根接种鉴定的结论.  相似文献