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Preparation on the basis of Trichoderma asperellum in the system of biological protection of wheat from Fusarium ear scab
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During the last century, as the area of wheat grown under advanced grain husbandry has increased worldwide, so too has the importance of Fusarium ear scab (FES) (synonym, Fusarium head blight) caused by several species of the fungus Fusarium. Yield losses due to FES can total 20%-40% and more depending on climatic conditions. During the last twenty years epidemics of FES in cereals have become chronic all over the world, including the United States and Russia. The most destructive of t… 相似文献
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链霉菌182-2抗真菌活性物质的分离及抑菌特性的初步研究 总被引:2,自引:0,他引:2
[目的]链霉菌182-2的发酵液对烟草赤星病有良好的抑制作用,本文拟对其发酵液中抗真菌活性组分进行初步分离,并进行抑菌特性研究.[方法]发酵液经预处理后,依次用活性炭脱色,大孔树脂吸附层析分离纯化,对纯化后活性物质(组分Ⅱ)的抑菌活性进行测定.[结果]分离纯化结果表明其活性物质中至少含有两种抗真菌活性组分.离体条件下当活性物质浓度为2 000μg/mL时,对烟草赤星病菌有很强的抑菌作用,抑菌圈直径达58.0mm.目测法测定抗生作用表明其最小抑菌浓度(MIC)为80 μg/mL,最小杀菌浓度(MBC)为160μg/mL.48 h时对菌丝生长和孢子萌发的抑制率最高,抑菌中浓度(EC50)分别为31.9 μg/mL和42.2μg/mL.活性组分处理还可导致病原菌菌丝和孢子萌发产生的芽管变形扭曲或产生大泡囊.[结论]纯化后的活性物质对烟草赤星病菌具有较强的抑制作用,有进一步研究利用的价值. 相似文献
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Kamanga-Sollo E White ME Chung KY Johnson BJ Dayton WR 《Domestic animal endocrinology》2008,35(3):254-262
Androgenic and estrogenic steroids enhance muscle growth in animals and humans. Estradiol-17beta (E2) and trenbolone acetate (TBA) (a synthetic testosterone analog) increased IGF-I mRNA expression in bovine muscle satellite cell (BSC) cultures. The goal of this study was to evaluate the mechanisms responsible for this increase by evaluating the effects of ICI 182 780 (an E2 receptor antagonist), flutamide (an androgen receptor inhibitor), G1 (a GPR30 agonist), and BSA-conjugated E2 on E2 and/or TBA-stimulated IGF-I mRNA expression in BSC cultures. Flutamide completely suppressed TBA-stimulated IGF-I mRNA expression in BSC cultures. ICI 182 780 did not suppress E2-stimulated IGF-I mRNA expression and 100nM ICI 182 780 enhanced (93%, p<0.05) IGF-I mRNA levels in BSC cultures. G1 (100nM) stimulated IGF-I mRNA expression (100%, p<0.05) but had no effect on proliferation in BSC cultures. E2-BSA, which cannot cross the cell membrane, stimulated IGF-I mRNA expression (approximately 100%, p<0.05) in BSC but even at extremely high concentrations had no effect on proliferation. In summary, our data indicate the E2-stimulation of proliferation and E2-stimulation of IGF-I mRNA expression in BSC cultures occur via different mechanisms. Our previous results showing that ICI 182 780 inhibited BSC proliferation and results of the current study showing lack of response to E2-BSA or G1 suggest that E2-stimulated proliferation in BSC cultures is mediated through classical estrogen receptors. Stimulation by ICI 182 780, G1 and E2-BSA suggests the E2-stimulated IGF-I mRNA expression in BSC cultures is mediated through the GPR30 receptor. 相似文献
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HAO Rui-min XIE Shu-yang HAN Wan-hong YAN Yun-fei SUN Yun-xiao YUE Zhen MA Ying LI You-jie 《园艺学报》2017,33(11):1987-1992
AIM: To investigate the effect of cepharanthine on the growth of human lung carcinoma A549 cells and the underlying mechanism. METHODS: After A549 cells were treated with cepharanthine, the growth inhibitory rate was detected by MTT assay. The cell morphological changes were observed under light microscope. The apoptosis of the A549 cells was analyzed by flow cytometry. The expression of microRNA (miR)-150, miR-182, p53 mRNA and FOXO1 mRNA were detected by real-time PCR. The downstream target genes were predicted by software, and the expression of p53 and FOXO1 was determined by Western blot. RESULTS: After cepharanthine treatment, the growth of A549 cells was inhibited, the apoptosis rate was significantly increased, and the expression levels of miR-150 and miR-182 were significantly decreased. With cepharanthine treatment at 10 μmol/L, the expression levels of p53 and FOXO1 were elevated; however, with cepharanthine at 30 μmol/L, the expression levels of p53 and FOXO1 were decreased. After transfection with miR-150, the expression of p53 was significantly decreased, while the expression of FOXO1 was significantly decreased after transfection with miR-182. CONCLUSION: Cepharanthine inhibits the growth of A549 cells and promotes the apoptosis of A549 cells by inhibiting the expression of miR-150 and miR-182. miR-150 and miR-182 may down-regulate the expression of p53 and FOXO1, respectively. 相似文献
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为加快甘蔗新品种推广力度,本试验开展了甘蔗新品种柳城03/182在百色蔗区的新植性能试验。结果表明:柳城03/182较新台糖22号表现为早熟、高糖,有效茎数多,但茎径、单茎重不及新台糖22号,比新台糖22号增产3.2%、增糖12%,但差异不显著。柳城03/182适合在百色市蔗区大面积推广应用,有利于改良百色市甘蔗不同熟期品种结构,改变品种单一化格局,为糖厂榨期的合理安排提供有利条件。 相似文献
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优质中筋小麦晋太182高产栽培技术研究 总被引:2,自引:2,他引:0
为了在山西中部麦区大面积推广优质中筋小麦晋太182,开展了播期和种植密度二因素随机区组试验研究。结果表明,播期与密度对晋太182产量构成因素的影响均达极显著水平,随着播期的推迟,株高依次降低,穗长依次缩短,结实小穗数和不孕小穗数均逐渐减少;随着种植密度增大,植株依次增高、穗长变短、结实小穗数减少、不孕小穗数增多。不同的播期有不同的最佳种植密度。晋太182适播期为9月24—29日,而以9月24日为最佳播期,适宜密度为375万~525万株/hm2,450万株/hm2为最佳密度。晋太182在因前茬作物不能及时收获而晚播的条件下,通过增大种植密度同样可以获得高产。 相似文献
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景电灌区土壤盐渍化的防治 总被引:1,自引:0,他引:1
本文根据实地调查、定位观测与室内分析的大量资料,提出景电灌区土壤盐渍化的成因、特性及其防治对策。这对灌区盐渍化土壤的综合治理、开发和预测预报都有重要的参考价值。 相似文献
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阐述了小麦新品种涡麦182的选育方法及选育经过,并介绍了涡麦182产量表现突出、产量结构合理、籽粒商品性好、综合抗性好等特点,提出了其栽培技术,以促进该品种的推广应用。 相似文献