洛阳市合欢主要病虫害防治技术     

沈改霞  赵丽 《中国园艺文摘》2011,27(3):70-71

合欢是重要的园林观赏树种。文章主要介绍合欢的五种常见病虫害及其防治措施。  相似文献   

高粱光敏色素A基因的克隆、蛋白结构与长短日照诱导表达模式     

王平  王春语  赵子良  丛玲  陆晓春 《分子植物育种》2020,(6):1748-1754

光敏色素是一类红光/远红光受体,对植物光形态建成、外部形态和生理功能等方面起着重要的调节作用。为明晰光敏色素A在调控高粱光形态建成发育过程中的作用,本研究比较分析光温敏感程度不同的高粱品种Btx623、Rio、Tx430和LH645的PhyA基因序列和PHYA蛋白的保守结构域,利用qRT-PCR明确长短日照条件处理下PhyA基因的表达模式。结果表明,Btx623、Rio和Tx430之间PhyA基因序列存在非同义突变,LH645在PhyA基因的第7个外显子上插入了5 bp碱基序列;高粱PhyA蛋白中含有1个PAS-2结构域、1个GAF结构域、1个Phytochrome结构域、2个PAS结构域、1个组氨酸激酶A结构域和1个类似组氨酸激酶的ATP激酶结构;遗传进化树分析表明,单子叶和双子叶植物PhyA蛋白明显聚为2大类,高粱PhyA、谷子PhyA和玉米PhyA1、PhyA2聚为一个亚类,相互间亲缘关系较近,与水稻PhyA同源性相对较远;在短日照(SD)条件下各时期PhyA基因转录水平变化显著,Rio PhyA基因转录水平较Btx623和Tx430变化明显,LH645 PhyA基因转录变化不显著,在长日照(LD)条件下Rio PhyA基因转录水平低于Btx623和Tx430,LH645 PhyA基因转录水平变化不显著。以上结果表明,PhyA受光周期诱导,推测其在高粱光形态建成中起重要调控作用。本研究为进一步解析PhyA基因功能及其在调控高粱光形态建成发育过程中的作用提供理论基础。  相似文献   

不同药剂处理对灵武长枣坐果与光合指标的影响     

宋丽华  万仲武  曹兵 《经济林研究》2016,(1):40-44

为了提高灵武长枣坐果率和单果质量,采用以色列专用肥(0.3%)、赤霉素(30×10-6)、爱乐壮(ALA、5-氨基乙酰丙酸)喷施处理大田种植的灵武长枣,测定其坐果率、光合速率、叶绿素含量、吊果量、单果质量等指标,分析不同药剂处理对灵武长枣坐果与光合作用的影响。结果表明:1‰ALA处理下的坐果率提高近1倍,且叶绿素含量、单果质量均高于对照;以色列肥处理下灵武长枣叶片的水分利用效率比对照高40%左右。因此推荐将1‰ALA和以色列专用肥应用于灵武长枣的生产实践中。  相似文献   

南方酸化红壤钾素淋溶对施石灰的响应   总被引：1，自引：1，他引：1  

周玲红  黄晶  王伯仁  李冬初  柳开楼  韩天富  张会民 《土壤学报》2020,57(2):457-467

为探究石灰施用的长期和短期效应对酸化红壤钾素的影响,依托始于1990年的国家红壤肥力与肥料效益监测长期定位试验,选取化肥氮磷配施(NP)、氮磷钾配施(NPK)、氮磷钾配施+半量秸秆还田(NPKS)及其增加常量石灰（NPL、NPKL、NPKSL）6个处理。室内土柱淋溶试验设置0 L、0.5 L、1 L和1.5 L石灰施用量,监测田间和淋溶后0 ~ 50 cm土层速效钾和缓效钾含量、pH及淋溶液中钾离子（K⁺）含量的变化。结果表明：1）施用石灰4年后,与NPKS、NPK、NP相比,各处理均增加了相应土层的缓效钾含量;NPKSL和NPL处理分别增加了0 ~ 40 cm和0~10 cm速效钾含量,增幅分别为2.06 % ~ 36.39 %和27.26 %。2）石灰施用量相同,各处理土壤累积K⁺淋溶量由大到小依次为NPKS处理、NPK处理和NP处理。施用石灰减少了NPKS和NPK处理淋溶液中累积K⁺含量,降幅为18.10 % ~ 57.70 %,且K⁺淋溶率也下降。3）施石灰提高了表层土壤pH;土壤中钾素盈余情况下,石灰当季施用量每增加1 000 kg·hm^-2,K⁺淋溶损失率降低11.7%;施用石灰和施肥是显著影响平均淋溶K⁺量和K⁺累积淋溶量的主效应。可见,施用石灰的短期和长期效应均能提高表层土壤pH;减少速效钾在剖面的运移,增加剖面下层缓效钾的含量;土壤淋溶K⁺量、累积K⁺淋溶量和K⁺淋溶率均随土壤中速效钾含量的增加而增加,随施用石灰而降低。合理的石灰用量能够有效降低酸化红壤K⁺淋溶损失风险。  相似文献   

Sinomenine reduces MPP⁺-induced damage in SK-N-SH cells via ANRIL/miR-626 signaling pathway     

HUO Ying-hao  WANG Jin  YIN Li-xin 《园艺学报》2000,36(8):1404-1413

AIM To investigate the effect of sinomenine (SN) on the damage of human neuroblastoma SK-N-SH cells induced by 1-methyl-4-4 phenylpyridine (MPP⁺) and its mechanism for exploring the pathogenesis of Parkinson disease. METHODS SN was used to treat MPP⁺-induced SK-N-SH cells. The levels of malondialdehyde (MDA) and glutathione (GSH) in cell culture supernatants were measured by ELISA. The apoptosis was analyzed by flow cytometry. The protein expression levels of Bcl-2 and Bax were determined by Western blot. The expression levels of long noncoding RNA ANRIL and microRNA-626 (miR-626) were detected by RT-qPCR. Dual-luciferase reporter assay was used to evaluate the relationship between ANRIL and miR-626. After ANRIL small interfering RNA was transfected into SK-N-SH cells, the effects of ANRIL expression knock-down on MPP⁺-induced SK-N-SH cell apoptosis, the protein expression levels of Bcl-2 and Bax, and the levels of MDA and GSH in cell culture supernatants were examined. RESULTS After treatment with MPP⁺, the apoptotic rate, Bax protein level and ANRIL expression in SK-N-SH cells were increased (P<0.05), and the Bcl-2 protein level and miR-626 expression were decreased (P<0.05). The level of MDA in cell culture supernatants was increased (P<0.05), and the level of GSH was decreased (P<0.05). After SN treatment or ANRIL expression knock-down, decreased apoptotic rate, Bax protein level and ANRIL expression (P<0.05), and increased Bcl-2 protein level and miR-626 expression in MPP⁺-induced SK-N-SH cells were observed (P<0.05). The level of MDA in the cell culture supernatants was decreased (P<0.05), and the level of GSH was increased (P<0.05). CONCLUSION SN attenuates MPP⁺-induced damage in SK-N-SH cells by regulating ANRIL/miR-626 signaling pathway.  相似文献   

Progress in roles of MNX1 antisense RNA 1 in malignant tumor     

GUAN Cang-hai  ZHAO Yu-qiao  Guo Liang  CHEN Yu-Zhu  WANG Wei-Na  JIANG Xing-ming 《园艺学报》2000,36(10):1908-1912

MNX1 antisense RNA 1 （MNX1-AS1） is a newly discovered long non-coding RNA （lncRNA）, which is highly dysregulated in various carcinomas and its expression level is closely related to the overall survival and prognosis of patients. MNX1-AS1 regulates the occurrence and development of carcinomas by endogenous competitive adsorption of miRNA, regulating cell cycle, inducing epithelial mesenchymal transformation and activating multiple signaling pathways. The in-depth study of the carcinogenesis of MNX1-AS1 is useful for the early diagnosis, targeted therapy and prognostic assessment of relevant carcinomas. This article reviews the roles of MNX1-AS1 in malignant tumor.  相似文献   

Effect of lncRNA FEZF1-AS1 on viability and apoptosis of LPS-induced vascular endothelial cells by regulating miR-363-3p expression     

ZHANG Si-min  DU Wen-hui  WANG Min  CHEN Fen  CAI Xiao-fang 《园艺学报》2000,36(11):1945-1951

AIM To investigate the mechanism of long noncoding RNA （lncRNA） FEZF1-AS1 regulating microRNA-363-3p （miR-363-3p） on the viability and apoptosis of lipopolysaocharide （LPS）-induced vascular endothelial cells. METHODS Human umbilical vein endothelial cells （HUVECs） were cultured in vitro. pcDNA-NC, pcDNA-FEZF1-AS1, anti-miR-NC, anti-miR-363-3p, miR-NC and miR-363-3p mimics were transfected into the HUVECs and LPS stimulation was applied for 24 h. RT-qPCR was used to detect the expression of FEZF1-AS1 and miR-363-3p. The cell viability was measured by MTT assay. The apoptotic rate was analyzed by flow cytometry. The dual-luciferase reporter experiment was used to verify the targeted regulation of FEZF1-AS1 and miR-363-3p. Western blot was used to determined the expression of cyclin D1, Ki67 and cleaved caspase-3. RESULTS Compared with control group, the expression level of FEZF1-AS1 in LPS group was significantly reduced （P<0.05）, and the expression level of miR-363-3p was significantly increased （P<0.05）. Compared with pcDNA-NC+LPS group, the cell viability in pcDNA-FEZF1-AS1+LPS group was significantly increased （P<0.05）, the apoptotic rate was significantly reduced （P<0.05）, the protein levels of cyclin D1 and Ki67 were significantly increased （P<0.05）, and the protein level of cleaved caspase-3 was significantly reduced （P<0.05）. Compared with anti-miR-NC+LPS group, the cell viability in anti-miR-363-3p+LPS group was significantly increased （P<0.05）, the apoptotic rate was significantly reduced （P<0.05）, the protein levels of cyclin D1 and Ki67 were significantly increased （P<0.05）, and the protein level of cleaved caspase-3 was significantly reduced （P<0.05）. Dual-luciferase reporter experiment confirmed that FEZF1-AS1 targeted miR-363-3p. Compared with miR-NC+pcDNA-FEZF1-AS1+LPS group, the cell viability in miR-363-3p+pcDNA-FEZF1-AS1+LPS group was significantly reduced （P<0.05）, the apoptotic rate was significantly increased （P<0.05）, the protein levels of cyclin D1 and Ki67 were significantly reduced （P<0.05）, and the protein level of cleaved caspase-3 was significantly increased （P<0.05）. CONCLUSION Over-expression of FEZF1-AS1 promotes the viability and inhibits apoptosis of LPS induced vascular endothelial cells by inhibiting the expression of miR-363-3p.  相似文献   

节能日光温室番茄长季节高产栽培研究初报   总被引：10，自引：1，他引：9  

刘富中  张志斌  贺超兴  王耀林  王怀松  朴俊峰  郑文义 《中国蔬菜》2000,1(2):6-0

对日光温室番茄长季节栽培进行了品种筛选及施肥指标和环境调控的研究 ,筛选出适宜日光温室番茄长季节栽培的品种 4个 ,提出 6 6 7m2 产量 2 0 0 0 0kg番茄的复合肥和钾肥施肥指标及采用遮阳网、反光幕、防虫网、诱虫色板、膜下滴灌等环境调控技术和措施。  相似文献   

河龙区间坝系水资源开发前景分析     

左仲国  姚党生 《中国水土保持》2002,(11):23-24

据研究 ,黄河中游在暴雨 (2 4h)频率设计标准为 1%～ 0 5 %的条件下 ,坝地总面积达到坝控流域面积的 1/ 2 0～1/ 10时可达到坝系相对稳定。河龙区间在坝系达到相对稳定后 ,通过坝系拦蓄作用 ,每年可提供 16亿～ 2 9亿m3 的农业用水 ;有 42 4%的年份年径流大部分可被拦蓄利用 ;有 5 7 6%的年份坝系土壤水分补充量可达 5 2亿m3 以上  相似文献   

东北日光温室番茄长季节栽培管理专家系统的研制     

邱华锐罗新兰  李国春薛俊华 花妍钟岩  姚振坤 《计算机与农业》2007,(12):45-47

本文根据温室番茄长季节栽培技术经验，利用农业专家系统开发平台（单机版）PAID4．0，开发温室番茄长季节栽培管理专家系统。本系统包括番茄栽培管理、病虫害防治模块、248条规则。通过将专家研究成果与计算机的结合为温室番茄长季节高效栽培技术的推广提供了广阔的应用前景。  相似文献