Zerumbone attenuates MPP⁺-induced cytotoxicity in human neuroblastoma SH-SY5Y cells by inhibition of oxidative stress     

CAO Rui-ping  WANG Jiao  WANG Ce 《园艺学报》2018,34(6):1061-1066

AIM: To investigate the role of zerumbone (ZER) in 1-methyl-4-phenylpyridinium (MPP⁺)-induced cytotoxicity of human neuroblastoma SH-SY5Y cells. METHODS: Human neuroblastoma SH-SY5Y cells were cultured in vitro and the protective effect of ZER against MPP⁺-induced cytotoxicity was measured by CCK-8 assay. Flow cytometry was used to determine the apoptosis and reactive oxygen species (ROS). The expression of Parkinson disease protein 7 (PARK7) was knocked-down by using PARK7-specific short hairpin RNA (shRNA). The protein levels of PARK7, nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot. RESULTS: MMP⁺ remarkably reduced the cell viability in a dose-dependent and time-dependent manner. The SH-SY5Y cell injury model was established by treatment with MPP⁺ at 600 μmol/L for 24 h. ZER up-regulated the protein levels of PARK7 and Nrf2 (P<0.05), alleviated apoptosis (P<0.05), and reduced ROS production (P<0.05) in the SH-SY5Y cell injury model. Meanwhile, N-acetyl-L-cysteine (NAC) had the similar functions. Moreover, significant reductions in the protein levels of Nrf2 and HO-1 (P<0.05), and obvious increases in apoptosis (P<0.05) and ROS level (P<0.05) were demonstrated in PARK7-knockdown cells. CONCLUSION: ZER protects SH-SY5Y cells against MPP⁺-induced cytotoxi-city, which may be related to activation of PARK7/Nrf2/HO-1 pathway, and subsequent attenuation of oxidative stress and apoptosis.  相似文献   

The Use of a Metabolic Disorder Index as a Predictor for Metabolic Eliminations in Endurance Horses     

《Journal of Equine Veterinary Science》2017

The equine endurance race involves both aerobic and anaerobic metabolisms of the horse. The intense physical activity over an extended period often causes susceptible horses to develop metabolic signs or problems resulting in elimination from races. Thus, the objective of this study was to develop a method for prediction and validation of a metabolic disorder index (MDI) to be used before endurance races. Three hundred seventy-five Arabian (n = 152) and Arabian cross (n = 223) endurance horses aged from 6 to 15 years and weighing between 350 and 450 kg were selected for the study in Malaysia. Blood samples were collected at pre- and post-race periods. The significant (P < .05) findings in horses with metabolic disorder were packed cell volume (0.50 ± 0.06 LL−1), creatine kinase (1,275.89 ± 121.45 UL−1), interleukin-6 (2.01 ± 0.89 ng/mL), decreased glutathione reductase (26.57 ± 3.95 ng/mL), and chloride (94.98 ± 8.12 mmol/L). A new method called MDI was developed as a predictor for horses with the potential to develop metabolic disorders in endurance races. The MDI indicated a higher value greater than 5.5 for those eliminated and lower value below 5.5 for those that completed the race successfully, this proved to be accurate in the prediction of metabolic disorder in endurance horses. The MDI is an innovative and simple method used as a prediction method that will assist the equine endurance society to reduce the rate of elimination and to safeguard against serious medical problems during endurance races in the tropics.  相似文献   

Genome-wide association mapping of phenolic acids in tetraploid wheats     

《Journal of Cereal Science》2017

Phenolic acids are major components of cell walls in wheat and have important implications on human health as antioxidants with anti-tumor activity. Our objectives were to identify phenolic acid genes in wheat by single nucleotide polymorphisms (SNPs) detected within the coding sequences of candidate genes, and to identify chromosomal regions associated with single phenolic acids and total soluble phenolic compounds. A set of candidate genes involved in the biosynthesis of hydroxycinnamic acid derivatives were identified by comparative genomics. SNPs found in the coding sequences of six genes (PAL1, PAL2, C4H, C3H, COMT1 and COMT2) were used to determine their chromosomal location and accurate map position on two reference consensus linkage maps. The genome-wide association study (GWAS), based on genotyping a tetraploid wheat collection with 81,587 gene-associated SNPs, detected 22 quantitative trait loci (QTL) distributed on almost all durum wheat chromosomes. Two QTL for p-coumaric acid were coincident with the phenylalanine ammonia-lyase (PAL2) and p-coumarate 3-hydroxylase (C3H) genes on chromosome arms 2AL and 1AL, respectively. The availability of candidate gene-based markers can allow elucidating the mechanism of phenolic acids accumulation in wheat kernels and exploiting the genetic variability of phenolic acids content for the nutritional improvement of wheat end-products.  相似文献   

脂肪细胞膜免疫降脂的研究     

姚华  王俊东  李宏全 《四川畜牧兽医》2003,30(9):24-26

本文综述了利用脂肪细胞膜免疫技术进行畜禽脂肪代谢研究的成果，其技术路线主要包括试验途径和方法、免疫机理、降脂效果及特异性脂肪膜蛋白的研究启示，从而为脂肪细胞膜免疫降脂试验提供成功的范例和科学的依据，并开辟降低脂肪、提高瘦肉率研究的新领域。  相似文献   

高原地区肉鸡能量、氮和氨基酸代谢率测定及其方法的研究   总被引：11，自引：1，他引：10  

武秀云  柴沙驼  王万邦  刘书杰  谢敖云  赵月萍  仇桂芳  张晓卫 《青海畜牧兽医杂志》2001,31(5):3-5

采用经典全收粪代谢试验方法和Sibald强饲快速法，在青海省西宁地区测定了爱拔益加肉仔鸡的能量、氮和氨基酸代谢率，并对肉用公鸡和蛋用公鸡的代谢率进行了比较。结果表明，高原地区肉用公鸡生长期（29-60日龄）的能量、氮和氨基酸代谢率随日龄的增加而降低；日粮能量、蛋白水平对其能量代谢率无影响，对氮和氨基酸代谢率有影响。35-38日龄和57-60日龄期间，肉用公鸡的能量代放率和总氨基酸代谢率显著低于蛋用公鸡；4-5月龄肉用公鸡的表观能量代谢率显著低于蛋用公鸡。未去盲肠公鸡对豆粕、肉骨粉、脱毒菜籽饼的氨基酸表观和真代谢率高于去盲肠鸡；公鸡去盲肠后对低纤维素日粮中的消化率影响较大；内源物矫正对鸡代谢试验测定结果具有可靠性和精确性。用体外胃蛋白酶法测定的三种蛋白质饲料的氨基酸代谢率与体内法具有较好的回归关系。  相似文献   

In ovo embryotoxicity of α-endosulfan adversely influences liver and brain metabolism and the immune system in chickens     

Pushpanjali  R.L. Prasad  S.K. Singh  S.B. Jadhao 《Pesticide biochemistry and physiology》2005,82(2):103-114

There is a lack of laboratory-based embryonic chicken toxicity studies with the ecologically relevant low dose/s of endosulfan that utilizes a more practical approach such as the chorioallontoic membrane (CAM) injection. In this investigation, 2μg AR grade α-endosulfan/egg (40% of LD₅₀ for embryos) was injected through the CAM in 12-day-old chicken embryos and the activities of glucose-6-phosphatase (G6Pase, EC 3.1.3.9), fructose 1,6-diphosphatase (FDPase, EC 3.1.3.11), adenosine triphosphatase (ATPase, EC 3.6.1.3) and succinic dehydrogenase (SDH, EC 1.3.99.1) and DNA and RNA content in liver and brain tissues and acetyl cholinesterase (AChE, EC 3.1.1.7) in the latter were determined at 24, 48, and 72 h post-exposure. The wet weight of the embryos did not differ between groups. Following endosulfan exposure, except increase in the hepatic ATPase activity (P < 0.01), there was a significant decrease in the following parameters: G6Pase activity in both the liver and brain (P < 0.01), SDH activity in the brain (P < 0.01), brain overall DNA and RNA concentration (P < 0.05), brain AChE activity (P < 0.01). Exposure of 18-day-old embryos to 2-μg endosulfan for 24 h caused decrease (P < 0.01) in the lymphocyte count and IgG content. Histopathology of thymus and bursa of Fabricius revealed a reduction in the population of thymic follicles, smaller thymocytes with the clear vacuoles in cytoplasm and fewer bursocytes accompanied by infiltration of erythrocytes in lymphoid follicles of the endosulfan-treated embryos. It was inferred that in ovo injection of 0.041 μg/g egg weight of α-endosulfan suppress gluconeogenesis (main energy source in embryonic life), nerve transmission, and immunity.  相似文献   

Minimal Set of Metabolic Pathways Suggested from the Genome of Onion Yellows Phytoplasma   总被引：1，自引：0，他引：1  

Kenro OSHIMA  Shin-ichi MIYATA  Toshimi SAWAYANAGI  Shigeyuki KAKIZAWA  Hisashi NISHIGAWA  Hee-Young JUNG  Ken-ichiro FURUKI  Masaki YANAZAKI  Shiho SUZUKI  Wei WEI  Tsutomu KUBOYAMA  Masashi UGAKI  Shigetou NAMBA 《Journal of General Plant Pathology》2002,68(3):225-236

  相似文献   

Role of Akt signal pathway in inhibition of neuronal apoptosis by Chinese medicine Zuogui pill in adult rats treated with monosodium glutamate     

GAO Chang  WANG Jing-zhou  WU Wei-kang 《园艺学报》2004,20(11):2016-2019

AIM: To explore the role of Akt signal pathway in apoptosis of neural cells in adult rats treated with Zuogui Pill, a Chinese medicine. METHODS: Flowcytometry and Western blotting methods were used to investigate the changes of cellular apoptosis rate and Akt signal pathway. RESULTS: Monosodium glutamate (MSG) could increase cellular apoptosis rate and significantly restrained the phosphorylation of Akt (Ser473) and Akt (Thr308), and markedly increased the levels of phospho-FKHR (ser256), GSK-3β (Ser9) and PTEN. Zuogui Pill partly inhibited the above effects of MSG. CONCLUSION: Zuogui pill effectively inhibits the neural apoptosis induced by MSG, and Akt pathway is involved in the neuronal protection of Zuogui pill.  相似文献   

Identification of Differentially Expressed Genes and Lipid Metabolism Signaling Pathways between Muscle and Fat Tissues in Broiler Chickens     

Min Zhang  Dan Zheng  Zhimei Peng  Yuting Zhu  Rongrong Li  Qiong Wu  Yan Li  Hongyi Li  Weihua Xu  Mao Zhang  Qichun Hung  Yanfa Sun 《The Journal of Poultry Science》2021,58(2):131

In this study, signaling pathways and key differentially expressed genes (DEGs) involved in lipid metabolism in muscle and fat tissues were investigated. Muscle and abdominal fat tissues were obtained from 35-day-old female broilers for RNA sequencing. DEGs between muscle and fat tissues were identified. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of DEGs were performed. A total of 6130 DEGs were identified to be significantly enriched in 365 GO terms, most of which were involved in biological processes, cellular components, and molecular functions in muscle and fat tissues. Three important lipid signaling pathways (pyruvate metabolism, the insulin signaling pathway, and the adipocytokine signaling pathway) were identified among the fat and muscle tissues of broilers. The key common DEGs in these pathways included phosphoenolpyruvate carboxykinase 2 (PCK2), acetyl-CoA carboxylase 1 alpha and beta (ACACA and ACACB), and the mitogen-activated protein kinase (AMPK) gene family. Hence, our findings revealed the pathways and key genes and gene families involved in the regulation of fat deposition in the muscle and fat tissues of broilers.  相似文献   

Influence of paricalcitol on renal tubulointerstitial fibrosis in diabetic nephropathy     

WANG Lai-liang  LUO Qun  CAI Ke-dan  ZHOU Fang-fang  GAO Yan-hong 《园艺学报》2015,31(4):719-724

AIM: To investigate the effect of paricalcitol (P) on renal tubulointerstitial fibrosis and the underlying mechanisms in diabetic nephropathy (DN).METHODS: DN rat model was induced by a single intraperitoneal injection of streptozotocin after fasting. The animals were randomly divided into 2 groups:the DN rats in paricalcitol-intervened group (group P) were injected intraperitoneally with paricalcitol dissolved in propylene glycol after the day when the model was induced successfully at a dose of 0.4 μg/kg (3 times a week); the DN rats in DN group (group D) were given isopyknic propylene glycol. Normal control group (group C) was also set up. The samples of blood, urine and renal tissue were collected after intervention of paricalcitol for 12 weeks. The biochemical indexes were measured. The renal tissues were used for pathologic observation and determining the expression of transforming growth factor-β1 (TGF-β1), Wnt-4, β-catenin and Klotho by immunohistochemistry and Western blotting. In addition, the correlation among the above indexes was analyzed.RESULTS: (1) Scr, BUN and 24 h urine protein increased significantly in group D compared with group C, while decreased in group P compared with group D (P<0.05). (2) The area of renal tubulointerstitial fibrosis increased in group D compared with group C, while decreased in group P compared with group D (P<0.05). (3) The expression of Klotho decreased, while the expression of TGF-β1, Wnt-4 and β-catenin increased in group D compared with group C (P<0.05). Compared with group D, the expression of Klotho increased, while the expression of TGF-β1, Wnt-4 and β-catenin decreased in group P (P<0.05). (4) The expression of Klotho was negatively correlated with the fibrosis area, TGF-β1, Wnt-4 and β-catenin (P<0.05).CONCLUSION: Paricalcitol inhibits renal tubulointerstitial fibrosis in DN by promoting the expression of renal Klotho, and inhibiting Wnt/β-catenin signaling pathway activation and TGF-β1 synthesis.  相似文献