Molecular based assessment of genetic diversity within Barbary fig (Opuntia ficus indica (L.) Mill.) in Tunisia     

Néjia Zoghlami  Ichraf ChritaBadra Bouamama  Mahmoud GargouriHassène Zemni  Abdelwahed GhorbelAhmed Mliki 《Scientia Horticulturae》2007

In this work, we report for the first time on the analysis of genetic diversity within a set of 36 Tunisian Opuntia ficus indica (L.) Mill. ecotypes using RAPD markers.  相似文献   

食用仙人掌肉质茎总DNA的提取   总被引：1，自引：1，他引：0  

王铁固  黄群策  陈秋芳  郭群召 《河南农业科学》2006,(1):84-86

以食用仙人掌的幼嫩肉质茎和紫糯玉米的幼嫩叶片为试验材料,采用CTAB法提取DNA,用紫外分光光度计检测DNA的浓度和琼脂糖凝胶电泳检测DNA的质量。结果表明,采用CTAB法可以从食用仙人掌中提取到一定质量和浓度的DNA,但是在操作过程中利用Tris-饱和酚对其进行纯化处理是非常必要的。  相似文献   

霸王树的抗盐性研究     

耐德.  A 邹韫 《干旱区研究》1992,9(3):31-35

鉴于在干旱区农业中利用盐水资源的需求,我们对霸王树在水培生长中的抗盐性进行了研究。NaCl浓度范围为5(对照)至200molm~(-3)。结果表明,叶状枝的生长对盐敏感,在盐浓度为50molm~(-3)时,生长量为对照的60%。根茎比仅在浓度为200molm~(-3)时才显著降低。对其它不同指标也进行了研究,例如水分含量、Na、K及Cl含量、渗透压和CO_2吸收等。这些指标中,叶状枝水分含量和CO_2吸收的降低均与叶状枝生长降低相联系。含盐量增高使叶状枝渗透压增加,这同组织脱水有关。我们认为霸王树在盐水胁迫下不产生渗透压的调节作用。  相似文献   

仙人掌多糖结构分析及抗凝血活性研究     

蔡为荣  谢亮亮  汤坚 《安徽农业科学》2012,40(4):2310-2314

[目的]分析仙人掌多糖组分和结构,并研究其抗凝血活性。[方法]以米邦塔仙人掌为试验材料,通过热水浸提、醇沉制得粗多糖CP,经交换层析和凝胶层析纯化,研究其单糖组成和重均分子质量分布与主链结构,并对多糖组分抗凝血活性进行了初步研究。[结果]对仙人掌粗多糖分离纯化后共得到3个多糖组分WSP1、WSP2a和WSP3,重均分子量分别为2.32×106、1.24×106和7.92×106。对氨基苯甲酸(PMP)衍生化高效液相色谱法检测表明,WSP1主要成分为半乳糖;WSP2a由鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖、葡萄糖醛酸和半乳糖醛酸组成,含量分别为6.3%、32.3%、12.9%、1.5%、4.8%、37.1%、0.64%和4.4%;WSP3主要成分为阿拉伯糖、半乳糖和木糖以及少量鼠李糖、甘露糖、糖醛酸。WSP2能延长活化部分凝血酶原时间(APTT)和凝血酶时间(TT),而对凝血酶原时间(PT)的影响不显著,说明其是通过影响内源性凝血系统而发挥抗凝血作用。纤维蛋白原转化纤维蛋白的抑制试验初步表明WSP2a和WSP3具有抗凝血活性。甲基化分析显示WSP2a含有1,4-连接的半乳糖酸,1,2-连接的鼠李糖和1,2,4-连接的鼠李糖构成的主链。[结论]该研究为仙人掌多糖资源优化和深度开发提供了试验依据。  相似文献   

仙人掌饮料研制工艺     

黄漫青  陈湘宁  刘可 《保鲜与加工》2005,5(4):15-17

以米邦塔食用仙人掌(Opuntia milpa alta)为主要原料,研制出风味独特的仙人掌饮料.仙人掌热烫温度为95℃,热烫时间为2 min,以0.1%碳酸钠、0.01%醋酸锌和0.2%抗坏血酸混合液作为护色剂,护色效果最佳.仙人掌饮料的优选配方为仙人掌汁25%,柠檬酸0.3%,白砂糖4%,蜂蜜4%,CMC-Na0.2%.产品呈淡黄绿色,具备仙人掌特有的清香.  相似文献   

Nutritional and mineral content of prickly pear cactus: A highly water‐use efficient forage,fodder and food species     

Jesse A. Mayer  John C. Cushman 《Journal of Agronomy and Crop Science》2019,205(6):625-634

Increased demand for food requires us to investigate livestock forage and fodder crops that can be grown over a wide range of locations where their cultivation will not compete with that of the food supply. A large portion of the southwestern United States consists of underutilized semi‐arid land. Crops typically used for livestock fodder or forage have high‐water demands that make them uneconomical or unsustainable for semi‐arid and arid regions. The growth rate and low‐input requirements of prickly pear cactus (Opuntia ficus‐indica) make it an excellent candidate for forage or fodder supplementation or replacement in these regions. Previous reports about forage quality data on Opuntia have been scattered across multiple locations, growing conditions and cultivars. Here, we report on the forage quality and mineral content of Opuntia ficus‐indica grown under both field and greenhouse conditions. Crude protein was 71 and 264 g/kg of dry mass for field and greenhouse conditions, respectively. Field‐grown plants showed higher acid and neutral detergent fibre content than greenhouse‐grown plants reflecting higher cellulose, hemicellulose and lignin accumulation. Nutritional values were also compared to requirements of cattle to determine what deficiencies might need to be addressed through supplementation. These data suggest that Opuntia can be used in combination with other feed sources to reduce the demand of resource‐intensive forage crops for raising livestock in dryland areas.  相似文献   

TLR4–MyD88信号转导途径介导仙人掌多糖免疫调节的机制研究     

李恒  李锐  马景球 《湖南农业大学学报(自然科学版)》2021,47(4)

为研究仙人掌多糖(ODPs)对Toll样受体4(TLR4)及其转导的MyD88依赖性信号途径中下游重要元件髓样分化因子88(MyD88)、肿瘤坏死因子受体相关因子６(TRAF6)、IκB激酶β(IKKβ) mRNA和蛋白表达的影响,体外培养小鼠腹腔单核巨噬细胞(RAW264.7),用ODPs干预24 h,收集细胞,分别采用RT–PCR法和蛋白质印迹Western blot法检测TLR4、MyD88、TRAF6、IKKβ mRNA及蛋白表达。结果显示：中、高剂量(100、200 μg/mL)ODPs显著增强TLR4、TRAF6及IKKβ的基因表达及TLR4、MyD88、TRAF6的蛋白表达(P<0.05),且具有良好的量效关系;ODPs也显著降低了MyD88的基因表达(P<0.05),对IKKβ的蛋白表达量增强效果不显著。说明非炎性条件下ODPs可促使TLR4高表达,同时激活TLR4胞内信号转导的MyD88依赖性途径。  相似文献   

Variation in chemical composition and biological activities of two species of Opuntia flowers at four stages of flowering     

Imène AmmarMonia Ennouri  Bassem KhemakhemThabèt Yangui  Hamadi Attia 《Industrial Crops and Products》2012,37(1):34-40

The chemical composition of hexane extracts from flowers belonging to two species of prickly pear, Opuntia ficus-indica (L.) Miller and Opuntia stricta (Haw.) Haworth has been studied by gas chromatography-mass spectrometry in four developmental stages of flower: vegetative, initial flowering, full flowering, and post-flowering stages. Remarkable differences were noted between the flowers’ compositions. The main compounds were carboxylic acid (28-97%), terpenes (0.2-57%), esters (0.2-27%), and alcohols (<1.8%).The study of the biological activities showed that extracts were active in vitro towards four bacteria and two fungal strains. It exhibited remarkable activity against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli.Antioxidant activity of the flowers extracts was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical method.Our findings demonstrate the interest of Opuntia flowers extract as a source of bioactive substances and its potential preservative use in food.  相似文献   

米邦塔仙人掌对小鼠的抗疲劳作用研究     

梁桂英  莫金钢  栾添 《安徽农业科学》2010,38(34):19354-19355

[目的]探讨米邦塔仙人掌（Opuntia Milpa Alta）对小鼠的抗疲劳作用。[方法]小鼠随机分组,试验组每天灌胃米邦塔仙人掌提取物400 mg/kg体重,对照组灌胃等量生理盐水。21 d后进行抗疲劳指标测定。[结果]米邦塔仙人掌提取物能显著延长小鼠游泳力竭时间（P〈0.01）,能减缓运动后小鼠肝糖原与肌糖原的消耗（P〈0.05）,降低运动后血乳酸及血清尿素氮水平（P〈0.05或P〈0.01）。[结论]米邦塔仙人掌对小鼠具有明显的抗疲劳作用。  相似文献   

仙人掌挥发油化学成分GC-MS分析     

金华  马驰骁 《安徽农业科学》2010,38(24):13060-13061

[目的]为仙人掌植物的合理开发和综合利用提供科学依据。[方法]采用水蒸气蒸馏法,从仙人掌中提取挥发油,用气相色谱-质谱联用法（GC-MS）对其进行分析。[结果]鉴定了35种化合物,主要成分为植醇（36.57%）、雪松烯（10.89%）、匙叶桉油烯醇（8.35%）、香树烯（7.24%）、石竹烯（5.90%）、棕榈酸（5.45%）、愈创木烯（3.05%）、苯乙醛（2.74%）、己醛（2.36%）等。[结论]与文献报道相比,组分和百分含量均有差别。  相似文献