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本文用生物胶作载体,加1250ppm、2500ppm、5000ppm营养液分别配制成生物胶缓释肥,分别施于洋兰试管苗的生长基质中,用叶面积模型Y=-0.32+0.87X(X=1.d)定期测算叶面积增量,以研究生物胶缓释肥对洋兰生长量的影响.运用模糊正交设计分析表明,在施肥方法、营养液浓度和施加次数3个试验因子中,施肥方式是影响生长的主要因子.与直接施加营养液的传统施肥方法相比,生物胶2号缓释肥能有效地促进洋兰试管苗的生长,其平均叶面积生长量提高2倍以上,且肥效持续时间长,成本低廉,具有较高的实用价值. 相似文献
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建兰花叶病毒的分离,鉴定及检测研究 总被引:13,自引:0,他引:13
从石斛兰病株中获得一个病毒分离物,利用曼陀罗进行分离纯化和繁殖,并通过梯度离心获得提纯病毒,经生物学、血清学鉴定及电镜观察,鉴定该分离物属于建兰叶病毒。分别用纯化病毒及SDS-PAGE回收病毒外壳蛋白免疫家兔。获得2种特异性抗血清,并用SPA-ELISA方法对兰花样品进行了检测研究。 相似文献
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介绍了旌德县境内野生兰花分布、生存的生态环境:和生长状况,并对野生兰花资源破坏的原因进行分析,进一步提出了保护野生兰花资源的合理建议。 相似文献
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Orchids are currently the most valuable potted crop in the United States. To date, no studies focused on making possible the year-round greenhouse production of flowering nobile dendrobium orchids. This experiment was aimed at developing a strategy to defer flowering of nobile dendrobium orchids by holding them under low temperature. Mature Den. Red Emperor ‘Prince’ and Den. Sea Mary ‘Snow King’ were held at 10 °C for various durations (0, 4, 8, 12 or 16 weeks) after vernalization (4 weeks at 10 °C). Plants were forced in a greenhouse after holding. Time to flower, flower differentiation (flowering node percentage, number of aerial shoot and aborted bud) and flower quality (total flower number, flower diameter, flower number per flowering node and flower longevity) were determined. Increase of low temperature holding duration from 0 to 16 weeks extended time to flower up to 3 months and did not affect parameters of flower except producing larger flowers and reducing flower number per flowering node for Den. Red Emperor ‘Prince’. Notably, the flower longevity was not adversely affected. Defoliation was aggravated in Den. Red Emperor ‘Prince’ by longer duration of cooling and was considered a detrimental effect of low temperature holding. 相似文献
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兰科菌根真菌对石斛组培苗的接种效应 总被引:6,自引:0,他引:6
从野生球花石斛(Dendrobium thyrsiflorum)根系中分离到Dt1、Dt2、Dt5、Dt6、Dt7、Dt8 共6株真菌,将其接种到铁皮石斛(Dendrobium candidum)和球花石斛组培苗的根系上,定期测定石斛苗的株数、株高、叶数、新芽数等指标,并与不接种的石斛苗进行比较。试验结果表明,Dt5号和Dt8号真菌的接种效果最好,地上植株和地下根系生长均良好,株数增多、株高增加、落叶减少,新芽和新根萌发,因此它们对于石斛组培苗的成功栽培具有实际应用价值。 相似文献