番茄新品种新星101的选育     

黎振兴  郑锦荣  黄爱兴  张衍荣  陈治禧 《中国蔬菜》2002,1(1):21-22

新星 10 1是以高抗青枯病、抗病毒病的 114F64 2 株系为母本 ,优质、耐病的A4 5为父本杂交育成的一代杂种。果实圆形 ,单果质量 12 0g ,肉质紧实 ,耐贮运。经苗期接种鉴定表现抗青枯病和病毒病 ,在高温条件下能正常结果 ,抗逆性好。在多年种植番茄、青枯病和病毒病发生较严重的地区栽培 ,更能发挥它的优良特性  相似文献   

猪C1QTNF3基因可变剪接体的鉴定及介导miR-101调控成脂分化的研究     

杨阳  张雪莲  张万锋  李文霞  李文新  蔡春波  路畅  高鹏飞  郭晓红  李步高  曹果清 《畜牧兽医学报》2021,52(11):3042-3052

旨在研究猪C1QTNF3基因可变剪接体的特性及miR-101通过C1QTNF3基因促进猪脂肪SV细胞成脂分化的机制。本研究采集3头30日龄健康马身猪仔公猪心、肝、脾、肺、肾、胃、股二头肌、腰大肌、皮下脂肪和背部脂肪组织样品,首先利用RT-PCR技术和生物信息学方法对C1QTNF3基因的不同转录本进行扩增和生物学特性分析,采用qRT-PCR技术检测C1QTNF3基因不同转录本在猪组织中的表达变化。随后,利用生物信息学预测发现,C1QTNF3上游的调控因子是miR-101,采用双荧光素酶报告试验验证miR-101对C1QTNF3的调控作用。最后,利用油红O染色和qRT-PCR技术检测过表达miR-101对猪脂肪SV细胞成脂分化的影响。基因克隆得到猪C1QTNF3存在两个转录本C1QTNF3和C1QTNF3-1,其中C1QTNF3-1为新鉴定的转录本;测序分析显示,与C1QTNF3相比,C1QTNF3-1的第1外显子区域缺失了219个碱基,少编码73个氨基酸。进化树分析显示,猪C1QTNF3和C1QTNF3-1蛋白序列与人和马来亚穿山甲等物种的亲缘关系较近。C1QTNF3和C1QTNF3-1在猪各组织中均有表达,且在各组织中C1QTNF3-1的表达量均极显著高于C1QTNF3（P<0.01）。过表达miR-101极显著下调C1QTNF3 3'UTR区域的荧光素酶活性（P<0.01）和C1QTNF3 mRNA的表达（P<0.01）,同时增加了脂肪细胞成脂分化关键基因PPARγ、C/EBPβ、SREBP-1c和FABP4的mRNA表达量（P<0.01）。本试验成功克隆了猪C1QTNF3基因的两个可变剪接体,其中C1QTNF3-1在猪不同组织中均高表达,推测该转录本为基因发挥功能的主要亚型。并深入研究了C1QTNF3基因的上游调控因子,揭示了miR-101靶向C1QTNF3促进成脂分化的机制,丰富了C1QTNF3的生物学作用和调控网络。  相似文献   

Effects of Kocide 101® on the bean (Phaseolus vulgaris L.)‐Rhizobium symbiosis     

F. P. Baijukya  E. Semu 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(3):175-183

A glasshouse study was undertaken to investigate the effects of the copper fungicide Kocide 101 and its residues in soil on the growth, nodulation and nitrogen fixation of beans (Phaseolus vulgaris L.). The soil used was a sandy clay loam classified as Typic Rhodustalf. The bean variety SUA 90 was used as test crop. The bean rhizobia strains CIAT 899, PV, and a local isolate were used. Kocide 101 applied at the recommended rate (equivalent to 1.7 mg kg^‐1 soil) had no significant negative effects on the growth, nodulation or nitrogen fixation of bean plants. Higher levels of Kocide 101 significantly (P < 0.05) reduced plant growth, nodulation and nitrogen fixation. The bean plants inoculated with the “local isolate”; rhizobia had the highest dry matter weights, nodule numbers and nodule dry weights, and also had more N fixation. They were followed by those inoculated with the PV, strain and, lastly, those inoculated with CIAT 899. The growth and nodulation of bean plants were still curtailed by the Kocide 101 residues four months after the fungicide was first applied to the soil. Therefore, occurrence of high levels of Kocide 101 in soils can have long‐term effects on the performance of the bean‐rhizobia symbiosis.  相似文献   

Bioinformatic Analysis and Prediction of miRNA-101 Target Genes in Yak     

LING Xiao-xiao  WU Xiao-yun  LIANG Chun-nian  TANG Peng  WANG Hong-bo  BAO Peng-jia  GUO Xian  YAN Ping 《中国畜牧兽医》2017,44(9):2580-2586

  相似文献   

Consensus versus dissent: Democracy,pluralism and governance in Sāmoa     

Elise Huffer  Asofou So&#x;o 《Asia Pacific viewpoint》2003,44(3):281-304

Abstract: Consensus is a term widely used in Sāmoa and elsewhere in the Pacific. It is considered to be a key feature of decision‐making and a fundamental characteristic of fa’asāmoa and other Pacific ways of being and doing. In Sāmoa it has been a mark of the strength and continuity of the fa’a matai through the ages of colonialism, neo‐colonialism and globalisation. However, although it has evolved in many ways to take into account new social trends (for instance allowing children to take part in family discussions) and remains strong at the family and village level, its role at the national level has virtually disappeared. This is seldom stated publicly and consensus is still held up as a defining characteristic of Sāmoaness. At the same time, although the rise of dissent is given little acknowledgement, it permeates public life. One could assume, based on this result that the ideology of consensus is perpetuated in order to reduce national public debate on governance issues.  相似文献   

优质高产大穗型玉米杂交种豫单101的选育     

刘宗华  汤继华  陈伟程  胡彦民  季洪强  谭晓军 《河南农业科学》2005,(12):37-38

以自选系L17为母本,8085为父本杂交育成了优质、高产、多抗、大穗型玉米杂交种豫单101。2年省区试该品种平均产量8 542.50 kg/hm2,比对照增产11.7%,籽粒粗蛋白含量11.78%,粗脂肪3.81%,粗淀粉71.46%,赖氨酸0.33%,容重762 g/L。属高蛋白优质粮饲兼用型玉米新品种。  相似文献   

固氮球形芽孢杆菌与巨大芽孢杆菌的混合增效作用   总被引：5，自引：0，他引：5  

龙苏  李法峰  陈明  林敏 《核农学报》2000,14(6):337-341

球形芽孢杆菌Bs7与巨大芽孢杆菌Bm1 0 1混合培养时具有固氮增效作用。稀释 40 0倍的巨大芽孢杆菌的无菌上清液与球形芽孢杆菌共培养 ,同样能增强后者的固氮活性。Bs7(pAB5 76 )在纯培养时的nifA表达活性为 6 849 5Miller单位 ,与巨大芽孢杆菌上清培养液进行混合培养时的表达活性为 75 0 0 4Miller单位。上述结果表明 :某些微量非碳源的活性分泌物和某些固氮调节基因可能在固氮增效作用中起重要作用。在与水稻联合培养条件下 ,混合接种球形芽孢杆菌与巨大芽孢杆菌可以提高固氮菌在水稻根表的定殖能力  相似文献   

基于NH_2-MIL-101(Fe)过氧化物模拟酶特性测定葡萄糖          下载免费PDF全文

万路  黄玉明  奉萍 《西南大学学报(自然科学版)》2019,41(3):84-88

研究发现NH_2-MIL-101(Fe)金属有机框架材料可催化H_2O_2氧化3,3,5,5-四甲基联苯胺(TMB)显蓝色,表现出过氧化物模拟酶特性,而且在较宽的温度(4～80℃)及pH值范围(2～10)内保持其模拟酶活性,结合葡萄糖氧化酶,建立了测定葡萄糖的方法.在优化条件下,吸光度与葡萄糖浓度在0.75～50μmol/L范围内呈现良好的线性关系,对葡萄糖的检出限为0.75μmol/L.将本法用于血清中葡萄糖的测定,获得满意结果.  相似文献   

体外受精不同时间等因素对体外受精效果的影响     

佟桂芝  韩永胜  王洪宝 《中国奶牛》2010,(7):31-33

本试验对牛体外受精不同时间、不同的培养液成分和培养方法等对奶牛体外受精后的卵裂率、囊胚发育率的影响进行了研究。试验包括:(1)牛体外受精不同时间(8h、20h)对奶牛体外受精后的卵裂率、囊胚发育率的影响;(2)不同的培养液成分对奶牛体外受精早期胚胎发育的影响。研究结果表明:(1)牛体外受精时间20h对奶牛体外受精后的卵裂率(78%)好于体外受精8h组(76%),但囊胚发育率前者不如后者好(20.51%VS23.68%),两者间差异不显著(P0.05)。(2)作为早期胚胎的培养液IVD101、TCM199培养系的卵裂率分别为76%、74%,而囊胚率却分别为22.37%、22.97%,TCM199培养系好于IVD-101,但两者间差异不显著(P0.05)。  相似文献   

基于DeepLab v3+的葡萄叶片分割算法     

李余康  翟长远  王秀  袁洪波  张玮  赵春江 《农机化研究》2022,44(2):149-155

为解决自然光照环境下复杂背景葡萄叶片图像的自动分割问题,使用一种DeepLab v 3+语义分割算法,完成对葡萄叶片分割.该算法采用ResNet 101作为主干网络进行特征抽取;采用空洞卷积和编码模块进行多尺度特征融合,将ResNet的中间信息和编码模块的特征组合作为解码输入;采用上采样的方式进行尺度还原,得到语义分割...  相似文献