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采用沉淀法制备紫杉醇白蛋白微粒,采用高效液相色谱法(HPLC)测定紫杉醇质量浓度,以二氯甲烷为有机相,采用萃取法分离白蛋白微粒与游离药物,测定其包封率。结果表明:紫杉醇在10~100 mg·L-1范围内线性良好,平均回收率可达到97.6%~101.0%;有机溶剂萃取法测定紫杉醇白蛋白微粒包封率的平均回收率,达94.80%~101.67%。这种方法测定紫杉醇白蛋白微粒回收率准确可靠,适用于沉淀法制备的白蛋白微粒的包封率测定。 相似文献
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紫衫醇对小鼠不同组织巨噬细胞功能的影响 总被引：1，自引：1，他引：0
为了探讨紫杉醇(Paclitaxel,PA)对小鼠不同组织巨噬细胞增殖和功能的影响。无菌操作制备小鼠腹腔、骨髓和脾脏巨噬细胞,在体外细胞培养体系中分别加入不同浓度40、80、160 ng/mL的PA和脂多糖(LPS)共培养,采用MTT法检测细胞增殖,ELISA和Griess法分别检测细胞分泌TNF-α和NO量,瑞氏-吉姆萨染色法测定腹腔巨噬细胞吞噬能力。结果表明： PA促进腹腔巨噬细胞和LPS诱导的腹腔和骨髓巨噬细胞增殖,但抑制脾脏和骨髓巨噬细胞以及LPS诱导的脾脏巨噬细胞增殖;PA抑制不同组织巨噬细胞分泌TNF-α,但促进腹腔和脾脏巨噬细胞分泌NO;PA提高了腹腔巨噬细胞吞噬鸡红细胞的能力。PA对小鼠不同组织巨噬细胞的免疫功能均有影响,这为进一步阐明PA的免疫调节机理提供了新的理论依据。 相似文献
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[目的]研究药剂对棉铃虫繁育能力的影响,为安全有效的控制棉铃虫危害奠定理论基础.[方法]采用饲喂法处理棉铃虫成虫,对其产卵量、卵孵化率、实验前后成虫体重差、成虫寿命进行测定.[结果]甲氨蝶呤抑制棉铃虫产卵量效果优于紫杉醇,0.02;甲氨蝶呤处理的棉铃虫单雌产卵量(104粒)最低,比对照降低了72.48;;紫杉醇抑制卵孵化效果好于甲氨蝶呤,0.5;紫杉醇处理的卵孵化率为11.43;,平均校正不育卵率(89.19;)达到最高.甲氨蝶呤处理对雌虫的寿命比对照缩短l～2d.紫杉醇对棉铃虫雄虫有增重作用,而甲氨蝶呤对棉铃虫雌虫有减重作用.[结论]甲氨蝶呤和紫杉醇对棉铃虫繁育能力均有弱化作用,0.02;甲氨蝶呤弱化棉铃虫单雌产卵量效果最好,0.5;紫杉醇抑制卵孵化效果最佳. 相似文献
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[目的]为了探讨试验用和临床用紫杉醇对肝癌HepG2细胞凋亡诱导作用的差异。[方法]用不同浓度的试验用和临床用紫杉醇处理肝癌HepG2培养细胞后,通过显微观察和流式细胞术检测其细胞凋亡。[结果]临床用紫杉醇在终浓度为0.5、1.0和2.0 mg/ml时,24 h非常显著性诱导细胞凋亡,48 h诱导细胞凋亡更剧烈。试验用紫杉醇24 h在终浓度为2.5、5.0和10.0 mg/ml时,非常显著诱导细胞凋亡;当试验用紫杉醇浓度为2.5 mg/ml时,48 h诱导细胞凋亡更剧烈。当试验用紫杉醇浓度为5和10 mg/ml细胞凋亡率逐步变低,可能走向死亡。[结论]在诱导肝癌细胞凋亡中,试验用紫杉醇要比临床所用紫杉醇的浓度要高。 相似文献
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[目的]探讨处方中几种辅料的加入对紫杉醇（Tax）脂质体粒径大小及Zeta电位的影响。[方法]采用薄膜分散-超声法制备Tax脂质体,利用正交设计筛选出Tax脂质体的最优处方,并测定Tax脂质体的粒径及Zeta电位。[结果]脂质体粒径及稳定性与处方中所加油酸钠等辅料有显著关系,且随着辅料量的增加,脂质体粒径变小,Zeta电位值降低,稳定性提高。[结论]油酸钠等辅料能显著降低Tax脂质体粒径及Zeta电位值。采用优选工艺制备的Tax脂质体包封率高,粒径均匀,稳定性好。 相似文献
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AIM:To investigate the effect of metformin combined with paclitaxel on the viability and apoptosis of breast cancer cell line MCF-7 and its possible mechanism. METHODS:MCF-7 cells were treated with metformin at different concentrations (2, 5, 10, 20, 40 and 80 mmol/L) and in vitro cultured. The viability of MCF-7 cells was measured by MTT assay. Metformin at 2 mmol/L or paclitaxel at 2.4 mg/L alone or in combination was used to treat the cells, and compound C, an inhibitor of adenosine monophosphate-activated protein kinase (AMPK) signaling transduction pathway, was also used. The cells were divided into control group, metformin group, paclitaxel group, combination group, and combination +compound C group. The apoptosis of the cells was analyzed by flow cytometry. The expression of Bax, Bcl-2 and caspase-3 at mRNA and protein levels was determined by RT-qPCR and Western blot. The protein levels of AMPK and P21 were examined by Western blot. RESULTS:Metformin at different concentrations (2, 5, 10, 20, 40 and 80 mmol/L) significantly inhibited the cell viability in a concentration-dependent manner (P<0.05). Compared with control group, treatment with metformin at 2 mmol/L or paclitaxel at 2.4 mg/L alone or in combination significantly inhibited the cell viability, induced apoptosis (P<0.05), decreased the level of Bcl-2 (P<0.05), increased the levels of Bax and caspase-3 (P<0.05), and promoted the protein expression of AMPK and P21 (P<0.05). The effects of metformin and paclitaxel in combination were better than those of single drug treatment, while AMPK inhibitor weaken these effects. CONCLUSION:Metformin combined with paclitaxel inhibits the viability and induces the apoptosis of breast cancer MCF-7 cells by activating AMPK signaling pathway and regulating apoptosis signaling pathway. 相似文献
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WANG Bo JIANG Xiao-xiao PENG Yun-peng ZHUANG Yan SUN Xiao-qing ZHU Hai-tao 《园艺学报》2014,30(10):1896-1901
AIM:To investigate the effects of low-dose paclitaxel on the morphology of bladder after partial bladder outlet obstruction (BOO) in rats. METHODS:Healthy female Sprague-Dawley rats (n=30) were randomly divided into sham operation group, BOO group and low-dose paclitaxel group. The rats in BOO group and low-dose paclitaxel group received operation to establish an obstruction model, while the rats in sham group underwent sham operation. After operation, the rats in low-dose paclitaxel group received intraperitoneal injection of paclitaxel at a dose of 0.3 mg/kg twice a week for 4 weeks. At the same time, the animals in sham group and BOO group received the same volume of saline by intraperitoneal injection. Four weeks after operation, each rat was sedated and the bladder was weighted. Histological changes of the bladder were observed by HE staining. Collagen deposition in the bladder tissue was observed by Masson staining, and the fibrosis area was measured. The ultrastructure of the detrusor was studied by transmission electron microscopy. RESULTS:Compared with sham operation group, a significant increase in bladder weight (0.376 g±0.052 g vs0.112 g±0.014 g, P<0.05), the muscle hypertrophy, and a decrease in the percentage of collagen area ［collagen/(collagen+muscle), 29.66%±2.69% vs38.94%±3.67%, P<0.05］ was observed in BOO group. Under electron microscope, intracellular connection had more gap junction and desmosomes than intermediate junction. The cell gap widened with a large amount of collagen fiber. Compared with BOO group, low-dose paclitaxel group decreased bladder weight (0.215 g±0.025 g vs0.376 g±0.052 g, P<0.05) and improved the muscle hypertrophy. The percentage of the collagen area was also decreased (19.94%±1.90% vs29.66%±2.69%, P<0.05). The detrusor microstructure showed that the intermediate junction was characterized by a predominance among the intracellular connections, and the intercellular space contained less collagen fibers in low-dose paclitaxel group. CONCLUSION: Low-dose paclitaxel may ameliorate the morphological damage of the bladder and recover bladder function in the rats with BOO by slowing down the process of bladder fibrosis. 相似文献
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Fusarium mairei, a paclitaxel-producing fungal endophyte, its effects on taxoid synthesis in Taxus cells were investigated via adding the fungal endophyte culture supernatants (FECS) to the suspension cultures of Taxus cuspidata. The concentration of FECS was determined on its total carbohydrate. When 100 mL of Taxus cell suspension cultures were treated with several dosages of FECS (4, 6 and 8 g) at day10, the cultures treated with 6 g FECS produced the highest yield of paclitaxel (5.84 mg L−1), which was 1.8-fold the yield from controls (3.14 mg L−1). The major elicitor element in FECS was an oligosaccharide of 2 kDa. In addition, the cultures treated with 6 g FECS resulted in 25.86 mg L−1 of the precursor 10-deacetylbaccatin III (10-DAB) accumulation, which was 11 times that of control cultures (2.32 mg L−1), and 4.7 times higher than that of cultures treated with 200 μM methyl jasmonate (MJ) (5.43 mg L−1). These results indicate that FECS favors to stimulate 10-DAB accumulation more effectively than paclitaxel accumulation. 相似文献