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乙醇-硫酸铵双水相提取马齿苋总黄酮工艺研究 总被引：1，自引：0，他引：1
[目的]优化乙醇-硫酸铵双水相提取马齿苋总黄酮工艺。[方法]采用乙醇-硫酸铵双水相体系提取马齿苋中主要成分黄酮,以总黄酮得率为指标,采用单因素和正交试验,考察提取溶剂浓度、料液比、提取时间、提取温度对总黄酮提取工艺的影响,确定乙醇-硫酸铵双水相体系提取马齿苋总黄酮的工艺。[结果]试验最佳提取条件为脱脂马齿苋粉末2.0 g,乙醇用量27.50%(W)、硫酸铵16.00%(W)、料液比1∶20、温度60℃、时间40 min,在此条件下,马齿苋总黄酮提取率为7.23%。[结论]乙醇-硫酸铵双水相提取法提取效率高、操作简便,提取剂价格便宜,是一种很有发展前途的提取方法。 相似文献
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Ezekwe MO Omara-Alwala TR Membrahtu T 《Plant foods for human nutrition (Dordrecht, Netherlands)》1999,54(3):183-191
The uniqueness of purslane (Portulaca spp.) asthe richest vegetable source of omega-3 (-3)fatty acids is well documented. However, purslane hasnot been domesticated or fully evaluated for itsnutritive value. The objective of this study was todetermine the influence of planting date on chemicalcomposition of purslane accessions. Eight accessionsfrom different geographical locations were planted 12days apart, and whole plants harvested at full bloom. Chemical analysis (DM basis) of leaves showedsignificant differences among varieties for all thecharacteristics measured. Accession by planting dateinteraction influenced (p < 0.05) levels of crudeprotein, total lipids, and carbohydrate contents. Wild Greek accession had the highest, while aBeltsville (Maryland) wild type had the lowest crudeprotein content (27.1 vs 20.5%) at the secondplanting date. Crude protein, lipid and ash levelswere most influenced (p < 0.05) by planting date. Total lipids varied from 4.0–5.8% and 3.7–5.1% forthe first and second planting dates, respectively. Selected fatty acid content indicated significantly(p < 0.05) higher levels of 18: 26, and18: 33 in the Dutch Garden accession comparedwith other varieties. The Egyptian wild accession hadthe lowest level of 18: 33. The ratio of 3 to 6 acids, which ranged from 5.5 to22.3 indicated a highnutritive value of purslane compared to other oilcrops such as soybeans and perrilla. The high levelsof protein in purslane compete with those of othercommercially important vegetable crops. The studyshows that, in spite of its genetic diversity, purslaneremains one of the most abundant terrestrial vegetablesources of Omega-3 fatty acids and other essentialnutrients potentially beneficial for humans as well asanimals. 相似文献
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Ali I. Mohamed Ahmed S. Hussein 《Plant foods for human nutrition (Dordrecht, Netherlands)》1994,45(1):1-9
Purslane (Portulaca oleracea), grown under greenhouse conditions, was harvested at three growth stages and analyzed for total solids, total protein, ash, soluble carbohydrate, and fructose/fructane in whole plants, leaves, stems, and roots. Significant increases were observed in total solids and protein during plant maturation. Leaves had the highest amount of protein in the third growth stage (44.25g/100g dry matter). Roots showed a decline in protein level as the plant aged. Soluble carbohydrate was significantly higher in growth states 1 and 3. Significant variation among growth stages was found with regard to total phosphorous, calcium, potassium, iron, managanese, and copper. Total phosphorus (P) content in leaves was significantly higher than P found in stems and roots. Iron (Fe) content varied significantly among growth stages, and roots and leaves had the highest Fe content (121.47 and 33.21 mg, respectively). Significant accumulation of managanese (Mn) was found in different growth stages. Leaves and roots had significantly higher Mn content than stems. 相似文献
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为了检测发酵马齿苋对雏鸡白痢的防治效果,设置发酵马齿苋高、中、低剂量组,非发酵马齿苋高、中、低剂量组,氟苯尼考组与阳性、阴性对照组,检测各组人工感染雏鸡的发病数、死亡数和治愈数。发现4g/L发酵马齿苋与氟苯尼考对雏鸡白痢防治效果相当(P>0.05),两者与阳性对照组相比,在预防试验中能显著抑制雏鸡的发病率和死亡率(P<0.05);在治疗试验中能显著提高雏鸡的治愈率(P<0.05),并降低雏鸡的死亡率(P<0.05)。在雏鸡白痢防治上,4g/L发酵马齿苋效果明显,可代替氟苯尼考作为养禽业的新型绿色开口药。 相似文献
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为研究马齿苋对东乡黑鸡产蛋性能及大肠杆菌和乳酸杆菌的影响,试验选择产蛋高峰期体况良好东乡黑鸡48只,按体重和产蛋数量基本相同原则分为4个处理组,每个处理组3个重复,每个重复4只。分别饲喂添加0%、1%、2%和3%马齿苋的日粮,试验期为35 d。从第10天开始收集鸡蛋直至试验结束,并在第20、35天收集蛋鸡早晨的粪便测定其大肠杆菌和乳酸杆菌的含量。结果显示:(1)与对照组相比,日粮中添加2%和3%马齿苋组产蛋数提高49.5%和67.7%,添加1%、2%和3%马齿苋组产蛋率分别提高15.4%、23.1%、26.9%和料蛋比分别降低了16.6%、17.4%、17.6%,差异显著(P﹤0.05)。(2)与对照组相比,日粮中添加3%马齿苋组肠道乳酸杆菌提高一倍,添加3%马齿苋组肠道中大肠杆菌数降低57.9%,差异显著(P﹤0.05),且乳酸杆菌与大肠杆菌数比值比对照组提高了39.0%。综上,日粮中添加3%的马齿苋可以优化蛋鸡肠道中乳酸杆菌和大肠杆菌,提高蛋鸡的抗病能力。 相似文献
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Specific plants can remove salts from the soil and contribute to saline remediation in orchard intercropping. Determining the level of highest salinity that a salt-removing crop can withstand without reducing its yield is important for management. It is also important to know the critical hazardous level of saline irrigation water for the fruit trees.The objective of this study was to investigate the salt-removing capacity of purslane by studying different stress criteria and by tracking its salt removal from germination to harvest. Therefore, a pot experiment was performed by enhanced salinity levels.The results showed that purslane could cumulatively remove considerable amounts of salt from the soil if practical to cultivate as an intercrop all year round. In this regard, 6.5 dS m−1 can be concluded as the reasonable salinity level for the purslane managed to be intercropped in fruit orchards. 相似文献
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光照强度对马齿苋生长及光合特性的影响 总被引：2，自引：0，他引：2
为明确光照强度对马齿苋生长发育、品质及光合特性的影响,采用人工遮光的方法,于马齿苋苗期进行遮荫,透光率分别为100%,(50±5)%和(25±5)%。分别测定了马齿苋的形态指标、生理指标及光合参数。结果表明:在100%透光条件下,马齿苋植株地上部和地下部干鲜质量、植株高度、可溶性蛋白、可溶性糖含量和叶绿素a/b值均与25%透光率条件存在显著性差异。马齿苋光饱和点和光补偿点分别为2 000μmol/(m2.s)和84μmol/(m2.s)。因此,马齿苋在营养生长期需要强光照,正常光照强度有利于马齿苋生长。马齿苋经遮荫处理后,光补偿点由84μmol/(m2.s)降至25μmol/(m2.s),说明其对光强具有较宽的适应性。 相似文献
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设施菜地揭棚休闲期种植填闲作物是阻控淋溶发生的重要措施，在降雨频繁地区氮磷阻控效率高的填闲作物尚不明确。以休闲为对照，种植5种填闲作物：高粱（Sorghum bicolor L.）、玉米（Zea mays L.）、黑麦草（Lolium perenne L.）、马齿苋（Portulaca oleracea L.）和羽衣甘蓝（Brassica oleracea var. acephala DC.），研究了不同种类填闲作物对设施菜地氮磷淋溶、土壤速效氮、土壤微生物的影响。结果表明：与休闲处理相比，种植填闲作物高粱、玉米、黑麦草、马齿苋和羽衣甘蓝分别显著减少了12.6、16.6、27.4、28.9和26.8 kg?hm-2的氮淋溶，显著减少了0.10、0.05、0.04、0.04和0.13 kg?hm-2的磷淋溶。氮淋溶阻控率由高到低依次为马齿苋、黑麦草、羽衣甘蓝、玉米、高粱，磷淋溶阻控率由高到低则依次为羽衣甘蓝、高粱、玉米、马齿苋、黑麦草。5种填闲作物中黑麦草、马齿苋和羽衣甘蓝的氮阻控率分别达到52.3%、55.1%和51.2%，较高粱和玉米氮阻控率平均提高了近一倍。这是因为黑麦草、马齿苋和羽衣甘蓝在休闲前期（21 d）就有一定的覆盖度，与休闲相比分别减少了23.5%、17.1%和26.7%的淋溶水量，而高粱和玉米前期覆盖度差，在前期降水多后期降水少的情况下其淋溶水量与休闲无显著差异；种植黑麦草、马齿苋和羽衣甘蓝耕层土壤（0~20 cm）中硝态氮的含量为40.1~52.8 mg?kg-1，而种植高粱和玉米土壤硝态氮的含量为67.8~72.7 mg?kg-1；此外，与高粱和玉米相比，马齿苋和羽衣甘蓝还显著提高了耕层土壤nirS型反硝化细菌和根际土壤中nosZ型反硝化细菌的丰度，可能增强了土壤的反硝化过程。综上，黑麦草、马齿苋和羽衣甘蓝作为降雨频繁地区的夏季填闲作物可显著减少氮磷淋溶，其中羽衣甘蓝对氮磷综合阻控的效果最佳。 相似文献
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