Quercetin activates mitochondrial autophagy via PINK1/parkin pathway to alleviate cerebral ischemia-reperfusion injury in rats     

WEI Si-can  LIN Tian-lai  HUANG Ling  SU Zhi-wei  WEI Mei-juan 《园艺学报》2021,36(12):2251-2257

AIM To analyze the regulatory effect of quercetin （QUE） on PTEN-induced putative kinase 1 （PINK1）/parkin mitochondrial autophagy pathway, and to explore the mechanism of quercetin in relieving cerebral ischemia/reperfusion （I/R） injury. METHODS Sixty SD male rats were randomly divided into sham operation group, model group （I/R group）, QUE group,3-methyladenine （3-MA） group and QUE+3-MA group. Administration started in each group 3 days before modeling, once a day, at 30 min after the last administration,except sham group, the other groups used 4-vessel blockage method to establish the whole brain I/R model. On the day after modeling, the neural function was evaluated by neuropathy disability score （NDS）. The volume of cerebral infarction was measured by 2,3,5-triphenyltetrazolium chloride （TTC） staining. The morphological changes of mitochondria in hippocampus were observed by transmission electron microscopy. The contents of interleukin-6 （IL-6） and tumor necrosis factor-α （TNF-α） in hippocampus were measured by ELISA. The activity of superoxide dismutase （SOD） and contents of malondialdehyde （MDA） in hippocampus were detected by xanthine oxidase method, thiobarbituric acid condensation method. Western blot was used to detect the proteinex pression of PINK1, parkin and LC3-II in brain tissue. RESULTS Compared with sham group, the hippocampus of the rats in I/R group and QUE+3-MA group showed swelling of mitochondria, destruction or disappearance of internal crista and other pathological damage,also the volume of cerebral infarction, the contents of IL-6, TNF-α and MDA, the protein expression levels of PINK1, parkin and LC3-II were increased （P<0.05）, while NDS score and activity of SOD were decreased （P<0.05）. Compared with I/R group and QUE+3-MA group, the pathological damage degree of hippocampus in QUE group was reduced, the volume of cerebral infarction, the contents of IL-6, TNF-α and MDA were decreased （P<0.05）, the proteinexpression levels of PINK1, parkin and LC3-II, and NDS score and activity of SOD were increased （P<0.05）.The above indexes in 3-MA group were opposite to QUE group. No significant difference in the above indexes between I/R group and QUE+3-MA group was observed （P>0.05）. CONCLUSION Quercetin activates mitochondrial autophagy and reduces cerebral I/R by regulating the expression of PINK1/parkin pathway proteins.  相似文献   

In vitro suppression of quercetin on hypertrophy and extracellular matrix accumulation in rat glomerular mesangial cells cultured by high glucose     

Tang DQ  Wei YQ  Yin XX  Lu Q  Hao HH  Zhai YP  Wang JY  Ren J 《Fitoterapia》2011,82(6):920-926

Quercetin's protective effects on the glomerulosclerosis of diabetic nephropathy (DN) in rat mesangial cells were investigated. The cell cycles, type IV collagen and laminin, TGF-β₁ mRNA, Smad 2/3 and Smad 7, and activities of cell antioxidases were measured. Compared with the high glucose group, quercetin may decrease the cell percentages of G₀/G₁ phase, Smad 2/3 expression, laminin and type IV collagen, and TGF-β₁ mRNA level significantly. The antioxidant capacity, the cell percentages of S phase and Smad 7 expression was significantly increased by quercetin. These results suggest that quercetin is a protective agent against glomerulosclerosis in DN.  相似文献   

植物雌激素槲皮素对非洲爪蟾生长和骨骼发育的影响     

丛琳  秦占芬  蒋湘宁  周景明  杨蕾  徐晓白 《勤云标准版测试》2005,(6)

用两栖动物非洲爪蟾作模型动物,来揭示植物雌激素槲皮素对非洲爪蟾生长和骨骼发育的影响。非洲爪蟾从46/47期开始,分别暴露50μg/L,100μg/L,200μg/L槲皮素,直至变态后1个月。结果表明槲皮素处理一方面促进蝌蚪体重的增加,另一方面导致尾的畸形。200μg/L槲皮素还导致脊柱的畸形,表现为少一块脊椎骨。  相似文献   

Subacute chlorpyrifos-induced oxidative stress in rat erythrocytes and the protective effects of catechin and quercetin     

Filiz Demir  Fatma Gökce UzunDilek Durak  Yusuf Kalender 《Pesticide biochemistry and physiology》2011,99(1):77-81

This study examined the effects of chlorpyrifos in the rat erythrocyte antioxidant system and evaluated the ameliorating effects of catechin and quercetin on the oxidative damage induced by chlorpyrifos. Sexually mature male Wistar rats were given chlorpyrifos (5.4 mg/kg, 1/25 of the oral LD₅₀), catechin (20 mg/kg), quercetin (20 mg/kg), catechin plus chlorpyrifos, and quercetin plus chlorpyrifos daily via gavage for four weeks. No statistical differences were found in the catechin-only and quercetin-only groups compared with the control group. By the end of the fourth week, chlorpyrifos alone increased the levels of malondialdehyde (MDA) and decreased superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities compared with the control group in rat erythrocytes. In the catechin-plus-chlorpyrifos and quercetin-plus-chlorpyrifos groups, there were statistically significantly decreased MDA levels and increased SOD, CAT, and GPx activities compared with the chlorpyrifos-only group. Thus, it appears that catechin and quercetin ameliorate chlorpyrifos-induced oxidative stress in rat erythrocytes in vivo.  相似文献   

Protective effect of quercetin on ER stress-related apoptosis induced by thapsigargin in macrophages     

YUE Wen  YAO Shu-tong  BAO Ying  WANG Jia-fu  YANG Na-na  SHANG Zhan-ping 《园艺学报》2012,28(3):518-523

AIM: To investigate the effects and possible mechanisms of quercetin (Que) on endoplasmic reti-culum stress (ERS)-related apoptosis induced by thapsigargin (TG) in RAW264.7 cells. METHODS: ER stress of RAW264.7 cells were induced by TG at concentration of 1 μmol/L for 24 h. After treated with different concentrations of Que (80, 120 and 160 μmol/L), the cell viability was determined by MTT assay.The apoptotic rate and the changes of intracellular Ca²⁺ concentration ([Ca²⁺]_i) were determined by flow cytometry, and the cell apoptotic morphology was observed under laser scanning confocal microscope.The protein levels of glucose-regulated protein 78 (GRP78) and C/EBP homologous protein (CHOP) were detected by Western blotting. The effect of Que on GRP78 and CHOP induced by TG with phosphatidylinositol 3-kinase (PI3K) inihibitor LY294002 at concentration of 15 nmol/L was measured by Western blotting. RESULTS: Que suppressed ER stress-related injury induced by TG in RAW264.7 cells. Compared with TG group, the cell viability increased (P<0.05), apoptotic rate and [Ca²⁺]_i decreased (P<0.05) and the changes of apoptotic morphology were alleviated. The increase in GRP78 and CHOP induced by TG as an ER stress marker was suppressed by Que (P<0.05). The suppressive effect of Que on GRP78 and CHOP was reproduced by LY294002 (P<0.05), but they failed to exhibit additive suppression. CONCLUSION: Que suppresses the ER stress induced by TG in RAW264.7 cells. The protective effect may be related to its suppression on PI3K signaling pathway.  相似文献   

桑叶中黄酮化合物的提取工艺及不同品种不同时期的含量变化   总被引：5，自引：2，他引：3  

张军  穆莉  檀华蓉  刘朝良  夏涛 《蚕业科学》2006,32(1):142-145

探讨了不同单因子对桑叶中黄酮成分浸提效果的影响。用80%乙醇作溶剂,原料质量浓度50.00 g/L,于70℃、pH 8的条件下浸提3 h并抽提2次的提取效果较好。以毛细管电泳仪测定、分析了不同品种、不同时期桑叶中的芦丁、槲皮素等成分,湖桑9001叶片中的芦丁含量高,湖桑32号的槲皮素含量高,10月份采摘桑叶中的芦丁和槲皮素的含量最高。芦丁、槲皮素等成分在桑叶中的动态变化:以10 mmol/L磷酸二氢钠-20 mmol/L的硼砂溶液(pH 8.62)作缓冲液,在25℃,20 kV的压力下电泳,245 nm波长处检测,线性关系良好,在8 m in内完全分离。  相似文献   

月月红中槲皮素·山奈酚的定性与定量研究   总被引：1，自引：1，他引：0  

王小宁  宋良科  王岩  王波  董关涛  汤昊 《安徽农业科学》2011,39(5):2662-2663,2682

[目的]通过对月月红中槲皮素、山奈酚的定性定量研究,拟建立其HPLC分析方法,为进一步研究民族民间药物月月红的药效学奠定基础。[方法]采用化学定性法确定月月红不同部位中的主要化学组分;色谱条件：Hypersil DJZ C18色谱柱（4.6 mm×200.0 mm,5μm）;流动相：甲醇-0.4%磷酸（60∶40,V/V）;检测波长：368 nm;流速：1.000 ml/min;柱温：30℃;理论塔板数按山奈酚和槲皮素峰计算不低于3 000,外标法计算。[结果]槲皮素在0.081 6~0.489 6μg线性关系良好（R2=0.999 6）,平均回收率为101.6%,RSD为0.87%;山奈酚在0.069 6~0.417 6μg线性关系良好（R2=0.999 6）,平均回收率为96.8%,RSD为1.01%。月月红叶中槲皮素为0.038 2%,茎为0.005 2%,全株为0.010 5%;月月红叶中山奈酚含量为0.027 6%,茎为0.002 1%,全株为0.009 6%。[结论]HPLC分析方法稳定、重复性较好;月月红不同部位均含有槲皮素、山奈酚,且叶含量高于全株和茎。  相似文献   

罗布麻中槲皮素提取工艺的研究     

许程剑  魏长庆 《安徽农业科学》2011,39(35):21679-21680,21684

[目的]研究罗布麻中槲皮素的最佳提取工艺。[方法]以新疆罗布麻茶叶为原料,采用单因素试验和响应面设计法优化超声波法提取槲皮素的工艺并建立数学模型。[结果]经响应面分析法优化后,槲皮素最佳提取工艺参数：浸提温度60℃、浸提时间35 min、液料比1∶20、浸提功率160 kW、乙醇浓度40%。[结论]在此工艺条件下槲皮素实际提取率为0.928%。  相似文献   

Simultaneous determination of kaempferol and quercetin in Heteropogon Contortus (L.) Beauv. by validated high-performance thin layer chromatography     

Navjot Kaur  Raghbir Chand Gupta 《Communications in Soil Science and Plant Analysis》2018,49(18):2247-2255

Presently, the fingerprint analysis of kaempferol and quercetin has been developed simultaneously via High-Performance Thin Layer Chromatography (HPTLC) from leaves, stem, and inflorescence of Heteropogon contortus. The HPTLC method for kaempferol and quercetin was optimized with the elution of toluene: ethyl acetate: formic acid (7:3:0.5 v/v) as a mobile phase. The fingerprint analysis of kaempferol and quercetin was developed at R_f values of 0.39 and 0.24, respectively, and densitometric evaluation was done at 254 nm. The linear regression data for the calibration curve of both the compounds show a good linear relationship in the concentration range of 2–12 nanogram spot^?1. The suggested method has been validated in terms of limit of detection (LOD) and limit of quantification (LOQ), precision, specificity, sensitivity, and accuracy. Present results show that maximum amount of kaempferol and quercetin is found in leaf extracts (35.80 and 17.01 milligram/gram of dry weight, respectively) of H. contortus.  相似文献   

HPLC测定蒲公英中槲皮素含量的研究     

肖奇志 《安徽农业科学》2010,38(30):16871-16872

[目的]建立蒲公英中槲皮素含量的测定方法。[方法]采用高效液相色谱法,AgilentC18柱（150.0mm×4.6mm,5.0μm）,流动相为甲醇-乙腈（63∶26）,紫外检测波长为370nm。[结果]槲皮素在0.1～1.0mg/ml范围内线性关系良好,平均回收率为98.37%。[结论]该方法分离好、快速、重复性好,可作为蒲公英的质量控制方法。  相似文献