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This study aimed to evaluate the effect of sorghum and sweet potato on the bioavailability of iron, gene expression of proteins involved in iron metabolism and the plasma antioxidant capacity in animals fed with whole sorghum grains processed by dry heat or extrusion, combined or not with sweet potato flour with high content of carotenoids. Five experimental groups were tested (n = 7): dry heat sorghum flour (DS); extruded sorghum flour (ES); whole sorghum flour + sweet potato flour (DS + SP); extruded sorghum flour + sweet potato flour (ES + SP) and positive control (FS). The evaluations included: hemoglobin gain, hemoglobin regeneration efficiency, gene expression of divalente metal transporter 1 (DMT-1), duodenal citochroma B (DcytB), ferroportin, hephaestin, transferrin and ferritin and total plasma antioxidant capacity (TAC). The ES + SP group showed higher (p < 0.05) expression of DcytB, ferroportin and hephaestin when compared to the control group. The DS group showed high (p < 0.05) expression of DMT-1 and the ES showed high mRNA expression of transferrin and ferritin. The changes in the sorghum physicochemical properties from extrusion process reduced the iron and phytate content, and increased the gene expression of proteins involved in iron metabolism, improving iron bioavailability. The combination of sweet potato and sorghum flour (dry or extruded) improved the iron capture and total antioxidant capacity, probably due to the presence of β-carotene and antioxidant compounds. 相似文献
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【目的】研究采用斜率比法对玉米、豆粕、菜粕和玉米酒精糟（distillers dried grains,DDGS）中胆碱的生物学效价（the relative bioavailability,RBV）进行评估。【方法】试验选用１日龄爱拔益加（ＡＡ）肉仔鸡132只,随机分为22个处理组,其中S1-S6组为标准曲线组,A1-A4组为玉米日粮组,B1-B4组为豆粕日粮组,C1-C4为菜粕日粮组,D1-D4为DDGS日粮组,每个处理6个重复,每个重复1只鸡。基础日粮采用醇提法将玉米、豆粕、菜粕和DDGS中的胆碱洗脱后配制而成。在基础日粮中添加不同剂量的氯化胆碱配制6种梯度的标准日粮,另将4种待测原料分别以10%、20%、40%和100%的比例替换洗脱过的相应原料配制试验日粮。分别以1-21日龄的平均日增重、肝脏脂肪含量和全血胆碱浓度为衡量指标,以比斜率法计算待测原料的相对生物学利用率。【结果】以平均日增重为指标,玉米、豆粕、菜粕和DDGS中胆碱RBV分别为63.95%、104.7%、37.98%和72.09%;以全血胆碱浓度为指标,玉米、豆粕、菜粕和DDGS中胆碱RBV分别为94.73%、184.11%、23.63%和134.23%。【结论】以日增重和全血胆碱含量为指标衡量饲料原料中胆碱生物学效价是可行的,但由于选择指标的不同,所得数值亦有所差异;4种参试原料中胆碱的相对生物学利用率从高到低依次为豆粕、 DDGS、玉米和菜粕。 相似文献
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