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西农萨能奶山羊血浆生长激素水平的变化及其与奶产量的关系 总被引：1，自引：0，他引：1
本实验用放免测定法(RIA)测定了38只西农萨能奶山羊(其中360～450日龄的青年羊10只,产前一月至产后90天的头胎和2胎羊各14只)血浆GH水平变化,分析了血浆GH水平与奶量的相关性。结果表明:360～450日龄的青年羊血浆GH维持在一个波动较小的范围(20.37±5.17～23.13±5.97ng/ml),头胎羊和二胎羊分娩前后及泌乳早期血浆GH水平的变化趋势相一致,在同一生理状态下,二胎羊血浆GH水平都显著地高于头胎羊。相关分析还表明:头胎羊和二胎羊血浆GH水平都与其奶量呈直线正相关关系(P<0.05)。综上结果可以认为,奶山羊血浆GN水平可作为选育高产奶山羊的一项指标。 相似文献
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JIN Li-qin LU Jian-xin YU Kang YUAN Qian XIE Ke-jian WANG Kai-fa ZHANG Yu-lan 《园艺学报》2000,16(8):726-729
AIM: To study the activation effects of maize pollen polysaccharides(PPM) on human thoracic cavity macrophage (hTMΦ). METHODS: Activities of lactate dehydrogenase(LDH) and acid phosphatase (ACP) in the hTMΦ were detected by automatic biochemical analyzer, and the level of tumor necrosis factor alpha(TNF-α) and interleukin-6(IL-6) in the hTMΦ was analyzed by radioimmunoassay, after hTMΦ were cultured with 0.312, 0.625, 1.250, 2.500, 5.000 mg/mL PPM-RPMI 1640 for 24 and 48 hours in vitro. RESULTS: The activities of LDH and ACP increased in the hTMΦ induced by PPM, and the levels of TNF-α and IL-6 in the hTMΦ induced by PPM increased markedly too. And the induced expression effect of TNF-α and IL-6 is associated with the concentration of PPM, and time for PPM inducing. CONCLUSION: PPM can induce cytokines secretion in hTMΦ, and activate hTMΦ in vitro. 相似文献
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用一种抗体同时测定植物粗提液中游离和结合脱落酸(Free ABA andConjugate ABA)的放射免疫分析方法测定了兴津温州蜜柑在人工抗寒锻炼和自然越冬过程中两种形态的ABA变化情况。结果表明,柑桔在抗寒锻炼和脱锻炼过程中,F-ABA和C-ABA有规律的变化与抗寒性的提高和丧失具有依存关系。 相似文献
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Sidikou DI Banga-Mboko H Tamboura HH Hornick JL Remy B Beckers JF 《Research in veterinary science》2006,80(3):260-266
The measurement of serum pepsinogen concentrations by enzymatic method and immunoassay provides diagnostic values and should be helpful in the detection of gastric diseases related to a rise of blood pepsinogen. In the present study, the correlation between a conventional enzymatic method and a recently developed radioimmunoassay (RIA) for serum pepsinogen A was investigated. A total of 123 sera samples of porcine foetuses (n = 28), adult healthy pigs (n = 56), pigs with parakeratosis (n = 25) and pigs with ulceration of the pars oesophagea (n = 14) were tested. Overall, there was a slight correlation between the two methods (r = 0.60). In relation to individual animal groups, the correlations (r) were 0.39 (P>0.05), 0.74 (P<0.001), 0.19 (P>0.05) and 0.34 (P>0.05) in foetuses, healthy pigs, pigs with parakeratosis and pigs with ulcers, respectively. In both methods, pepsinogen concentrations (means+/-SE) were significantly higher (P<0.05) in pigs with parakeratosis (1778 +/- 86.00 mUTyr/L; 690 +/- 53.00 ng/mL) and in pigs with ulcers (2026 +/- 153.00 mUTyr/L; 1747 +/- 94.00 ng/mL) when compared to healthy pigs (935 +/- 58.00 mUTyr/L; 275 +/- 35.00 ng/mL). The proteolytic method gave a significant increased activity (P<0.05) in foetuses (1150 +/- 82.00 mUTyr/L) vs. (935 +/- 58.00 mUTyr/L) in healthy adult pigs, indicating an additional proteolytic activity in the sera of foetuses or neonates. 相似文献
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A. Ayad N.M. Sousa J. Sulon J.L. Hornick M. Iguer-Ouada J.F. Beckers 《Research in veterinary science》2009,86(3):377-382
The measurement of serum or plasma PAG concentrations is currently used as a specific method for pregnancy diagnosis in cattle. In this study, the correlation between five radioimmunoassay systems (RIA-497, RIA-706, RIA-780, RIA-809 and RIA–Pool) developed for measurement of PAG concentrations in ruminant species was investigated in plasma from pregnant Friesian Holstein females. Plasma PAG concentrations (ng/mL) measured by different RIA systems were significantly correlated between them ( 0.81; P < 0.001). PAG concentrations increased significantly from Day 21 (n = 27) to 30 (n = 37) after AI by use of all PAG–RIA systems. From Day 30 to 80 after AI, lower PAG concentrations were observed when using the homologous system RIA-497. The addition of several proteinase inhibitors changed neither the non specific binding nor the B0 binding to the tracer. Our results suggest that all tested PAG–RIA (RIA-497, RIA-706, RIA-780, RIA-809 and RIA–Pool) are highly correlated and can be useful to follow PAG concentrations in samples collected during the first trimester of gestation. 相似文献
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用异源放射免疫分析法测定猪血清促黄体素（LH） 总被引：1，自引：0，他引：1
本实验用兔抗牛LH(bLH)血清,~(125)Ⅰ-标记牛LH(~(125)Ⅰ-bLH)建立猪LH(pLH)的异源放射免疫分析法。bLH、pLH的标准曲线及猪血清稀释曲线平行。此方法的特异性强,与猪促卵泡素(pFSH)的交叉反应小于0.5％;灵敏度为0.015～0.020ng/管;猪血清添加pLH的回收率为96.8±3.8％;测定内变异系数为3.3％(n=14),测定间变异系数为5.5％(n-5),用本方法测定,注射LRH-A_210分钟后猪血清LH的含量显著提高(P<0.01)。75、95、115、135、155日龄后备母香猪(n=7)血清中LH含量依次为1.50±0.59,1.54±0.42,1.76±0.30,1.41±0.17,1.88±0.30ng/ml;发情时母香猪(n=2)血清中LH含量为7.07±2.73ng/ml 相似文献
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目的观察半边莲生物碱对肾性高血压大鼠内皮素1 mRNA表达、蛋白合成和释放的影响。方法采用两肾一夹高血压大鼠模型,随机分为高血压组、半边莲组、卡托普利组和假手术组。用原位杂交技术观察大鼠外周血白细胞内皮素1 mRNA的表达,应用免疫组织化学染色计数大鼠主动脉内皮细胞铺片内皮素阳性细胞率(反映内皮素1蛋白的合成),用放射免疫技术测定大鼠血浆内皮素的含量。结果与假手术组比较,高血压组外周血白细胞内皮素1mRNA表达增强(34.64%±8.39%比9.34%±4.47%,P<0.05),动脉内皮细胞合成内皮素增多(7.42%±0.24%比1.58%±0.24%,P<0.05),血浆内皮素水平显著升高(221±24ng/L比138±19ng/L,P<0.05)。应用半边莲生物碱8周后,与高血压组比较,内皮素1 mRNA表达(20.38%±11.31%比34.64%±8.39%,P<0.05)、内皮素合成(3.53%±0.21%比7.42%±0.24%,P<0.05)和血浆内皮素水平(191±21ng/L比221±24ng/L,P<0.05)均受到显著抑制。结论肾性高血压大鼠伴有内皮素表达增强,半边莲生物碱能抑制内皮素基因的转录、蛋白合成及翻译,对防治肾性高血压所致的血管病变具有一定作用。 相似文献