便携式XRF仪现场快速测定土壤中Pb的可行性研究     

柳伟  许伟  孙东  赵松江 《安徽农业科学》2017,45(33)

便携式XRF分析仪具有轻便、快速和可测对象较多与测量元素较广等特点而广泛应用于矿石、土壤等方面快速检测,但土壤的现场快速测定结果的准确性有待进一步研究与探讨。以安宁河谷平原土壤中Pb为研究对象,通过对便携式XRF仪现场快速检测结果与实验室分析结果的对比和影响因素的分析,建立基于安宁河谷平原表层土壤快速测定结果的线性函数为y=1.170 9x-17.326 0(R~2=0.992 9,x为现场检测结果),探讨其影响因素,为该区域表层土壤中Pb快速检测结果提供依据。  相似文献   

烟青虫初孵幼虫的高效快速收集方法     

王博琳  常耘  司升云 《长江蔬菜》2016,(20)

比较了常用的烟青虫人工收集方法和新研发的烟青虫初孵幼虫快速收集方法的效果。试验结果表明,与人工收集方法相比,快速收集方法获得初孵幼虫所用时间少,收集的初孵幼虫存活率搞,达96.33%,且收集的幼虫发育更加整齐,雌、雄蛹单头质量(雌蛹295.33 mg,雄蛹284.66 mg)略大;还可节省大量人力,避免了人工挑虫对初孵幼虫的伤害,保障了烟青虫实验种群的大量优质扩繁。  相似文献   

薄荷的快速繁殖与栽培技术的研究   总被引：3，自引：0，他引：3  

马春红陈霞翟彩霞  郭秀林崔四平李广敏 《中国农学通报》2004,20(4):230-230

本文介绍了薄荷的特性、栽培技术及用途，表明它在我们日常生活中起着重要的作用。用薄荷的组织培养方法可加速其繁殖系数，以达到迅速应用的目的。  相似文献   

白菜型油菜DFR基因的克隆与序列分析     

李运涛  李加纳  柴友荣  杨成军  雷波 《分子植物育种》2005,3(4):485-492

By using RACE (rapid amplification ofcDNA ends) based homologous cloning strategy, we have successfully isolated the genomic and full-length cDNA sequences of a gene encoding typical DFR (dihydroflavonol-4-reductase) from black-seeded Brassica campestris L. var. oleifera DC.. The gene, designated BcDFR here, is 1 722bp in length and harbors 5 introns with typical splice sites of plant DFR genes. BcDFR cDNA is 1311bp in length with a 1 158bp ORF as well as a 25bp 5‘ UTR and a 128bp 3‘ UTR. The encoded BcDFR protein is 385 aa with a calculated Mw of 42.85kD and a pI value of 5.55. The nucleotide and amino acid sequences of this gene share extensive homologies to plant DFR genes of wide origins especially high similarities to Cruciferous DFR genes. Sequence analyses such as phylogenetic analysis, conserved domain search and substrate specificity region detection all indicated that BcDFR gene is a quite potentially biofunctional gene. Its cloning enables us to further dissect the possible relatedness between DFR gene and Brassica seed coat color traits and to create transgenic novel yellow-seeded rapeseed germplasm through antisense- or RNAi-suppression of DFR gene expression in black-seeded elite cultivars.  相似文献   

点云自适应切片方法研究   总被引：2，自引：1，他引：2  

任乃飞  胡汝霞  万俊 《农业机械学报》2006,37(2):118-121

在反求工程(RE)中,对点云模型进行曲面重构不仅花费大量的时间,而且要求操作人员具有丰富的经验。而对点云切片则可避免曲面重构,直接生成层片文件,输入到快速成型(RP)机进行加工。但是点云切片的一个难题就是如何确定每层切片的厚度以保证切片的形状精度在误差范围内,提出一种基于距离图像的点云自适应切片的新方法,能够获得在给定的形状误差范围内切片层的最大厚度。  相似文献   

A logistic analysis of the factors determining the decision of smallholder farmers to intercrop: A case study involving rubber–tea intercropping in Sri Lanka     

S.M.M. Iqbal  C.R. Ireland  V.H.L. Rodrigo 《Agricultural Systems》2006

The factors influencing the decision of smallholder farmers to adopt new farming technologies were studied with reference to rubber–tea intercropping in Sri Lanka. Rubber–tea intercropping has been recommended previously to rubber farmers as a means to improve productivity and income during the early pre-tapping phase of rubber growth. Although crop trials have shown that the two crops are agronomically compatible and potentially produce a combined economic yield superior to the yield of a sole crop grown on the same area of land, there is little evidence of widespread adoption of this practice among smallholder farmers in Sri Lanka. The aim of the study was to determine the major factors that influence the decision to undertake rubber–tea intercropping and to construct a predictive model that describes the likelihood of adoption of intercropping by traditional smallholder rubber growers. A rapid rural appraisal (RRA) was undertaken based on semi-structured interviews of 90 smallholder farmers in the main rubber growing low wet zone of Sri Lanka. Among a number of factors shown to significantly influence the decision to intercrop tea with rubber, three were shown to operate independently, namely level of income, source of income (i.e. solely from own farm or from farm plus additional off-farm enterprises), and availability of land considered suitable for tea cultivation. A statistical model developed through correlation and logistic analysis, which predicts the likelihood of a smallholder adopting intercropping based on these factors, is presented and discussed. The most likely combination of circumstances (82% probability) under which rubber–tea intercropping is practiced is shown to be where the farmer’s income is greater than Rs. 10,000 per month, where the farmer’s income is based solely on own farm enterprises, and where more than 80% of the farmer’s land area was judged to be suitable for tea cultivation. Conversely, 30% of smallholder farmers that chose not to intercrop did possess land suitable for tea cultivation. Qualitative responses to the RRA indicated that limitation of technical knowledge was the main problem subsequently faced by rubber farmers who had adopted rubber–tea intercropping. Results indicate that there is need for both income support through farm subsidies and further agricultural extension services, if rubber–tea intercropping is to be adopted more widely in Sri Lanka. The wider usefulness of the developed logistic model in determining the likelihood of adoption of intercropping by smallholder farmers is discussed.  相似文献   

Effect of Different Plant Growth Regulators on Callus Induction and in vitro Rapid Propagation of Wild Petunia Juss.     

Yan ZHAO  Na XU  Zhongyou MA  Wei LIU 《农业科学与技术》2012,(5):931-934

In this study,the seeds of wild Petunia Juss.were used as explants to investigate the optimal condition for tissue culture.Several different kinds and concentrations of growth regulators were adopted to produce more multiple bud clumps,callus or roots in this study.The experiments may provide experimental foundation for the rapid propagation technology and establishment of tissue culture system for wild Petunia Juss.  相似文献   

利用间歇浸没式生物反应器进行果蔗组培快繁     

杨柳  秦钢  杨丽涛  罗瑞鸿  游建华 《热带作物学报》2010,31(4):614-620

以果蔗(Badila)茎尖脱毒组培苗为材料,利用间歇浸没式生物反应器(Temporary immersion bioreactors TIBs)进行组培快繁的技术体系的研究。结果表明:(1)使用TIBs系统进行甘蔗组织培养一代增殖较传统方法高出10倍以上;(2)TIBs系统以第4代继代材料为宜;(3)接种密度在10～15株/L培养基最有利于甘蔗组培苗的增殖和生长;(4)TIBs系统中6-BA浓度为0.5～1.0mg/L时适合于增殖培养,NAA浓度为4mg/L时有利于根的诱导;(5)浸没间歇频率在1min/3h时增殖和生长表现较为优异,1min/6h时有利于根的诱导和根的生长。  相似文献   

中华芦荟的离体快繁技术研究     

宛淑艳  袁迎  陈春莲 《安徽农业科学》2009,37(10):4382-4383

[目的]探索芦荟的离体快繁技术,为芦荟的组织培养提供试验依据。[方法]以盆栽中华芦荟的嫩茎尖为外植体,MS为基本培养基,不同的增殖阶段添加不同的植物激素进行离体快繁试验。[结果]培养30 d后,3瓶被污染,4瓶不长愈伤组织,13瓶在芽基部产生圆球状浅黄色的愈伤组织。在诱导芽分化约20 d后,2瓶被污染,11瓶开始芽点分化。3.00 mg/L 6-BA的处理效果最佳。诱导生根11 d后,1瓶被污染,2瓶出现叶子干焦的现象,17瓶长势良好。中华芦荟愈伤组织诱导的适宜培养基为MS＋6-BA 2.50 mg/L＋NAA0.15 mg/L,诱导率为60%;丛生芽分化及继代的适宜培养基为MS＋6-BA 3.00 mg/L＋NAA 0.10 mg/L,诱导率为85%;生根的适宜培养基为MS＋6-BA 0.30～0.50 mg/L＋IBA 0.20 mg/L＋活性炭0.5%。[结论]该研究为芦荟的快繁提供了初步的理论依据。  相似文献   

粉红色索尔邦百合的组培快繁技术研究     

张伟丽  金欣庆 《北方园艺》2007,(4):212-214

研究了以索尔邦百合(Lilium Sorbonne)的鳞片叶为外植体的组培和植株离体再生技术,培养基采用MS基本培养基,附加不同的激素组合.分别获得诱导的愈伤组织、芽及根,并遴选出最佳激素组合,以确立索尔邦百合组织培养再生系统.试验结果表明适合的诱导培养基是MS BA0.5mg/L NAA 0.2mg/L;继代培养基为MS 2,4-D 1.0mg/L和MS BA 0.5mg/L NAA0.2mg/L;生根培养基是1/2MS IBA0.2mg/L和1/2MS NAA0.2mg/L.该方法最大增殖倍数达到5.74.  相似文献