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1.
We compared patterns of acorn dispersal and predation by wood mice among four tree species (Quercus serrata, Quercus crispula, Castanea crenata, and Juglans mandshurica var. sieboldiana) that are abundant in cool temperate woodlands. We devised an acorn dispersal experiment using 400 magnet-inserted acorns and a magnetic locator in a 1.8-ha study plot, which spanned a cut-over area and an adjacent deciduous forest. Ten wire mesh baskets, each containing 40 acorns (10 acorns per species), were placed on the border between these two habitat types. About 13.0% (n = 52) of the total acorns remained in the baskets, while 77.3% (n = 309) were dispersed throughout the study plot and subsequently retrieved using the magnetic locator. Microhabitat, distance, and burial depth of transported acorns were significantly different among species. In the cut-over area, J. mandshurica var. sieboldiana acorns were dispersed under fallen trees or branches and near stumps, and were buried deeply in the soil. Dispersal distances of J. mandshurica var. sieboldiana acorns were significantly greater than those of Q. serrata acorns. The number and microhabitat of transported acorns significantly differed between habitat types. J. mandshurica var. sieboldiana acorns were dispersed in the cut-over area rather than in the forest. For all four species, the numbers of acorns delivered to fallen trees or branches, stumps, and crumbled soil with overhang under any vegetation type were greater in the cut-over area than in the forest.  相似文献   
2.
为了给拟穴青蟹(Scylla paramamosain)苗种培育过程中饵料的合理投喂提供基础理论数据,采用投喂-饥饿和饥饿-投喂的处理方式对拟穴青蟹大眼幼体和Ⅰ期仔蟹分别开展营养储存饱和点实验(PRS)和不可恢复点实验(PNR),研究饥饿对大眼幼体和Ⅰ期仔蟹蜕皮和生长的影响。结果显示,大眼幼体的PRS实验中,投喂时间≤2 d时,大眼幼体的蜕皮率为0;投喂时间≥4 d时,大眼幼体的蜕皮率和发育时间与连续投喂组(F组)无显著性差异;但投喂4 d-饥饿组(F4S组)大眼幼体蜕皮后Ⅰ期仔蟹的增重率和个体大小均显著小于F组(P<0.05)。大眼幼体的PNR实验中,饥饿2 d时大眼幼体的存活率仅为(30.00±13.23)%。Ⅰ期仔蟹的PRS实验中,连续饥饿组(S组)Ⅰ期仔蟹的蜕皮率为0,而投喂2 d-饥饿组(F2S组)、投喂3 d-饥饿组(F3S组)和F组3组Ⅰ期仔蟹的蜕皮率均无显著性差异。投喂1 d-饥饿组(F1S组)和F2S组Ⅰ期仔蟹蜕皮后Ⅱ期仔蟹的增重率和个体大小均显著小于F组(P<0.05),且F1S组Ⅰ期仔蟹的发育时间显著延长(P<0.05)。Ⅰ期仔蟹的PNR实验中,除饥饿1 d-投喂组(S1F组)外,F组Ⅰ期仔蟹的蜕皮率显著高于其他处理组(P<0.05);各组Ⅰ期仔蟹蜕皮后Ⅱ期仔蟹的增重率与饥饿时间呈负相关,而发育时间却与饥饿时间呈正相关;饥饿时间≥2 d时,Ⅰ期仔蟹蜕皮后Ⅱ期仔蟹的个体大小明显变小。拟合分析结果显示,大眼幼体的PRS;值是3.57 d,Ⅰ期仔蟹的PRS;和PNR;值分别是0.90 d和2.01 d。研究结果表明,新蜕皮的大眼幼体必须给予充足的饵料才可确保高的存活率,大眼幼体投喂6 d后可以适当减少投喂量,短期饥饿对Ⅰ期仔蟹蜕壳和生长的影响相对较小,Ⅰ期仔蟹的耐饥饿能力强于大眼幼体。  相似文献   
3.
Wild‐caught and pond‐reared female mud crab Scylla serrata at different stages of ovarian maturation were collected from Samar and Capiz, Philippines. Crabs were categorized into five stages according to the external morphological and microscopic appearance of the most advanced oocytes. The ovaries, hepatopancreas, muscle and newly spawned eggs (NSE) were analysed for lipid class components and fatty acids. Total lipid was higher in pond‐reared than in wild‐caught crabs but increased with ovarian maturation in both groups. Ovarian lipid peaked at the fully mature stage, coinciding with a decline in hepatopancreatic and muscle lipids. Lipid levels declined significantly in spent females. The tissues contained elevated highly unsaturated fatty acids such as arachidonic (20:4n‐6), eicosapentaenoic (20:5n‐3) and docosahexaenoic (22:6n‐3) acids, but at higher levels in late maturing and fully mature ovaries and in NSE. The type of lipid class and fatty acid components in mature ovaries as well as in NSE are generally considered to be indicative of their importance in reproductive physiology and embryonic and larval development.  相似文献   
4.
This is an initial paper in a series of overviews of biological research and aquaculture development of the mud crab, Scylla serrata, in China. Results of experimental ecological studies on mud crabs are reported here. As a result of these experimental studies, results that are important for mud crab culture were also discovered and these include, methods to condition and manage broodstock, determination of ecological conditions that are suitable for embryonic development, and the influence of temperature, salinity, diet and larval density on development and survival of larvae. Results of this work will be useful in establishing a good method for artificial mass culture of larvae.  相似文献   
5.
亲蟹质量及亲蟹培育是锯缘青蟹人工育苗的基础。本文就亲蟹的选择、亲蟹暂养期的环境、亲蟹的饵料及饲养条件进行了探讨。在相同的理化因子条件下,海捕亲蟹的抱卵率(62.13%)大约为养殖亲蟹抱卵率(26.67%)的2.31倍。  相似文献   
6.
Cannibalism is one of the main causes of mortality in the culture of the mud crab Scylla paramamosain, particularly in the early post‐larval and juvenile stages when the densities of hatchery‐reared crabs may be very high before stocking into ponds or release into the wild for stock enhancement. In a series of experiments investigating cannibalism mitigation, the influence of stocking density, the effectiveness of sand substrate, brick and shell shelters and feed type were compared in culture of crabs from instar 1 for short nursery periods of 15–30 days. Inclusion of brick and shell shelters significantly increased survival over sand substrate alone. However, inclusion of shelters did not affect growth rates. In scaled‐up nursery production in lined‐ponds, with shelters, live Artemia biomass and fresh chopped shrimp or tilapia were found to be equally effective feeds for juvenile crabs stocked at a density of 70 m−2 from instar 1 and grown for 30 days [52–66% survival, 21.6–24.6 mm carapace width (CW)]. In an extended nursery period for a further 30 days, crabs of 22 mm CW, stocked at 30 m−2 in the same ponds, attained a final size of 34.5–36.2 mm CW with a survival of 64.3–67.0% using the same feeds.  相似文献   
7.
1999-2000年在温岭市的两家育苗生产单位进行工作了锯缘青蟹人工苗中间培育的研究。两年间共投入中间培育的大眼幼体864.2万只,培育出可供养殖放养的商品稚蟹(C3-C7)575万只,成活率达到66.54%,达到生产性育苗技术要求。  相似文献   
8.
Intensive nursery systems are designed to culture mud crab postlarvae through a critical phase in preparation for stocking into growout systems. This study investigated the influence of stocking density and provision of artificial habitat on the yield of a cage culture system. For each of three batches of postlarvae, survival, growth and claw loss were assessed after each of three nursery phases ending at crab instars C1/C2, C4/C5 and C7/C8. Survival through the first phase was highly variable among batches with a maximum survival of 80% from megalops to a mean crab instar of 1.5. Stocking density between 625 and 2300 m−2 did not influence survival or growth in this first phase. Stocking densities tested in phases 2 and 3 were 62.5, 125 and 250 m−2. At the end of phases 2 and 3, there were five instar stages present, representing a more than 20‐fold size disparity within the populations. Survival became increasingly density‐sensitive following the first phase, with higher densities resulting in significantly lower survival (phase 2: 63% vs. 79%; phase 3: 57% vs. 64%). The addition of artificial habitat in the form of pleated netting significantly improved survival at all densities. The mean instar attained by the end of phase 2 was significantly larger at a lower stocking density and without artificial habitat. No significant effect of density or habitat on harvest size was detected in phase 3. The highest incidence of claw loss was 36% but was reduced by lowering stocking densities and addition of habitat. For intensive commercial production, yield can be significantly increased by addition of a simple net structure but rapidly decreases the longer crablets remain in the nursery.  相似文献   
9.
10.
长柄石杉中石杉碱甲的含量测定   总被引:1,自引:0,他引:1  
[目的]建立HPLC法测定长柄石杉中石杉碱甲的含量。[方法]色谱条件:色谱柱为ZORBAX Extend-C18(150 mm×4.6 mm,4.5μm),流动相为乙腈-浓度0.02 mol/L磷酸二氢钾(8∶92,V/V),检测波长为310 nm,柱温为25℃,流速为1 ml/min,进样量为10μl。[结果]石杉碱甲在0.046~0.460μg/ml浓度范围内与峰面积呈良好线性关系(R=0.999 9),平均回收率为97.45%,RSD为2.70%,平均含量为0.025%。[结论]该方法简单、易行,可用于长柄石杉中石杉碱甲的含量测定。  相似文献   
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