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〔摘要〕目的研究盐酸青藤碱的透皮吸收及离子导入方法对透皮吸收的影响、方法采用家兔离体皮肤及
Franz扩散池进行体外透皮试验,并以电子治疗仪作为外加电场进行离子导入,观测盐酸青藤碱注射液的透皮渗透
率,并比较外加电场对其透皮渗透量的影响、结果盐酸青藤碱的透皮量随着时间的延长而增加,符合零级动力学
方程( R==0.952 1)、外加电场可增加其透皮吸收量,其中以电场强度20 mA、通电时间15 min的透皮量较为I}想、
结论盐酸青藤碱注射液可透皮吸收,电场导入对其透皮吸收有一定的促进作用、木研究为临床中以盐酸青藤碱注
射液进行离子导入的治疗方法提供了实验依据、 相似文献
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建立了以高效液相色谱法测定盐酸沃尼妙林关键中间体二甲基半胱胺截短侧耳素含量的检测方法。色谱条件为SepaxC18色谱柱(150×4.6mm,3μm)流动相为乙腈-磷酸缓冲液(pH2.5)(V/V= 40∶60), 流速为1ml/min,检测波长为205nm。 并对该检测方法进行了系统适用性、线性、精密度、重现性稳定性、检测限、定量限以及回收率的考察,结果均良好,可以用于盐酸沃尼妙林关键中间体的含量检测。 相似文献
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以阿苯达唑为原料,采用一锅合成法,通过氧化、酸化反应得到目标产物盐酸阿苯达唑亚砜。以丙酮为反应溶剂,当阿苯达唑和盐酸的投料比为30 g∶13 mL,阿苯达唑和双氧水的投料比为1∶1.01(n∶n),反应时间为30 min时,制备的盐酸阿苯达唑亚砜的收率为95.78%,通过IR、1H-NMR1、3C-NMR和ESI-MS分析对其产物结构进行了确证。 相似文献
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AIM To investigate the effect of sinomenine (SN) on the damage of human neuroblastoma SK-N-SH cells induced by 1-methyl-4-4 phenylpyridine (MPP+) and its mechanism for exploring the pathogenesis of Parkinson disease. METHODS SN was used to treat MPP+-induced SK-N-SH cells. The levels of malondialdehyde (MDA) and glutathione (GSH) in cell culture supernatants were measured by ELISA. The apoptosis was analyzed by flow cytometry. The protein expression levels of Bcl-2 and Bax were determined by Western blot. The expression levels of long noncoding RNA ANRIL and microRNA-626 (miR-626) were detected by RT-qPCR. Dual-luciferase reporter assay was used to evaluate the relationship between ANRIL and miR-626. After ANRIL small interfering RNA was transfected into SK-N-SH cells, the effects of ANRIL expression knock-down on MPP+-induced SK-N-SH cell apoptosis, the protein expression levels of Bcl-2 and Bax, and the levels of MDA and GSH in cell culture supernatants were examined. RESULTS After treatment with MPP+, the apoptotic rate, Bax protein level and ANRIL expression in SK-N-SH cells were increased (P <0.05), and the Bcl-2 protein level and miR-626 expression were decreased (P <0.05). The level of MDA in cell culture supernatants was increased (P <0.05), and the level of GSH was decreased (P <0.05). After SN treatment or ANRIL expression knock-down, decreased apoptotic rate, Bax protein level and ANRIL expression (P <0.05), and increased Bcl-2 protein level and miR-626 expression in MPP+-induced SK-N-SH cells were observed (P <0.05). The level of MDA in the cell culture supernatants was decreased (P <0.05), and the level of GSH was increased (P <0.05). CONCLUSION SN attenuates MPP+-induced damage in SK-N-SH cells by regulating ANRIL/miR-626 signaling pathway. 相似文献
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为检测麻杏石甘散、氟苯尼考注射液中非法添加的盐酸溴己新,甘草颗粒中非法添加的吲哚美辛,以十八烷基键合硅胶为填充剂,磷酸盐缓冲液-乙腈(20∶80)为流动相检测盐酸溴己新,以乙腈-0.1mol/L冰醋酸溶液(50∶50)为流动相检测吲哚美辛,流速为1.0 m L/min,波长扫描范围为200~400 nm,建立了相应的HPLC-PAD检测方法,并采用峰纯度检查和光谱相似度检查辅助对照品比对方法,对非法添加药物进行确证。结果显示,麻杏石甘散和氟苯尼考注射液中盐酸溴己新的回收率分别为94.8%和98.5%,甘草颗粒中吲哚美辛回收率为95.2%;盐酸溴己新线性方程为y=14780200x-16476,R=0.9999,吲哚美辛线性方程为y=10995430x+13033,R=0.9999;麻杏石甘散中盐酸溴己新检测限为83 mg/kg,氟苯尼考注射液中盐酸溴己新检测限为1.6 g/L,甘草颗粒中吲哚美辛检测限为1 g/kg。 相似文献
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盐酸环丙沙星在雏鸡体内蓄积毒性的研究 总被引：1，自引：0，他引：1
对盐酸环丙沙星在雏鸡体内的急性毒性进行了研究。结果ＬＤ５０为２８６２．８６ｍｇ／ｋｇ体重；蓄积系数Ｋ≥５．３，此时无一只雏鸡死亡，为轻度蓄积或基本无蓄积；雏鸡对本品产生明显耐受性，应用安全可靠。 相似文献
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AIM:To investigate the effect of sinomenine on the viability, migration and invasion of human ovarian cancer SKOV3 cells and its possible mechanism. METHODS:The SKOV3 cells were treated with sinomenine at different concentrations for 12 h, 24 h and 48 h. CCK-8 assay was employed to detect the effects of sinomenine on the viability of the SKOV3 cells. Flow cytometry was used to analyze the cell cycle distribution. The cell migration and invasion abilities were measured by Transwell assay. Western blot was used to determine the protein levels of cyclin A, cyclin D1, E-cadherin and matrix metalloproteinase-9 (MMP-9). RESULTS:Sinomenine remarkably inhibited the viability of SKOV3 cells and IOSE80 cells in a time-dependent and dose-dependent manner (P<0.05), and the IC50 values of 48 h were 2.12 mmol/L and 17.35 mmol/L, respectively. In a dose-dependent manner, sinomenine induced G0/G1 and S phase arrest in SKOV3 cells (P<0.05), suppressed the migration and invasion abilities of SKOV3 cells (P<0.05), down-regulated the protein levels of cyclin A, cyclin D1 and MMP-9 (P<0.05), and up-regulated the protein level of E-cadherin (P<0.05). CONCLUSION:Sinomenine inhibits the viability, migration and invasion of human ovarian cancer SKOV3 cells most likely via down-regulation of the protein levels of cyclin A, cyclin D1 and MMP-9, and up-regulation of the protein level of E-cadherin. 相似文献
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HPLC法青风藤根中青藤碱含量的测定及其提取工艺的优化 总被引：1，自引：1，他引：1
[目的]测定青风藤根中青藤碱的含量并与藤茎中含量比较,同时优化青藤碱的提取工艺,为合理有效地开发青风藤资源提供依据。[方法]用HPLC法测定青藤碱的含量,通过正交试验优化青藤碱的提取工艺。[结果]青风藤根中青藤碱的平均含量为1.73%,比藤茎中含量高0.62%;青藤碱的最佳提取工艺为：乙醇浓度为70%,乙醇用量为15ml,超声处理时间为30min,超声频率为30Hz。[结论]该试验比较了青风藤根和藤茎中青藤碱的含量差异,为合理有效地开发利用青风藤资源、评价青风藤药材提供了可靠的依据。 相似文献
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