猫鼻气管炎病毒、怀状病毒、泛白细胞减少症病毒的分离及其其形态学观察     

李六金  李成  姜焕宏  吴钟灵  胡淑贤  师长宏  张志培  李学忠  刘晓荣 《中国预防兽医学报》2001,23(5):341-345

根据病毒的理化特性，从猫三联活疫苗中分离出猫鼻气管炎、猫西洋太和猫泛白细胞减少症病毒；筛选出每种病毒敏感的细胞，并将该3种病毒分别分别在其中传代与扩增；通过电镜观察此3种病毒的形态学特征及其在宿主细胞内增殖部位、分布情况等，并以此对猫3种病毒进行了初步鉴定。  相似文献   

原木灵芝孢子粉和浸膏粉中的牛磺酸   总被引：1，自引：0，他引：1  

陈体强  徐洁  朱培根  李开本 《食用菌学报》2001,8(2):45-46

采用热水提取－磺基水杨酸沉淀法进行样品的前处理，利用氨基酸自动分析仪测定供试样品的牛磺酸含量。试验结果表明，原木灵芝孢子粉和破壁孢子粉中牛磺酸含量分别为8．44mg/100g-9.23mg/100g和7．34mg/100g-7.89mg/100g，而灵芝浸膏粉中牛磺酸含量高达140．82mg/100g-157.38mg/100g.  相似文献   

大豆高产理想株型的构成     

麻浩  李乐农 《湖南农业大学学报(自然科学版)》1996,22(3):309-314

根据大豆理想株型的概念，分析了大豆的主要生理性状和形态性状与产量的关系，并提出了大豆高产理想株型的合理构成，以期为大豆育种的田间选择和株型育种提供理论依据和实践指导。  相似文献   

湖南省滑胸茧蜂属Homolobus Foerster初报(膜翅目:茧蜂科:滑胸茧蜂亚科)     

游兰韶  周至宏  童新旺 《湖南农业大学学报(自然科学版)》1990,(4)

描记滑胸茧蜂属属征和褐滑胸茧蜂形态,并提供该蜂的形态图。  相似文献   

不同地区黑蚱蝉的形态结构与鸣声比较研究     

雷仲仁  李莉  闵一健  徐经平 《西北农业学报》1994,3(2):48-52

黑蚱蝉的鸣声和其发音器,具有基本的构造和特性,即种性.但也有一定的地理差异,鸣声有单音色,复合声,主峰频率高低等变化,这和形态的大小,发音器及其辅助发音器的变化相一致.黑蚱蝉的召唤声和求偶声是鸣声分类较理想的类型,而群鸣声不能用于种下分类.  相似文献   

Effect of various plant factors on the expression of creeping-rootedness in lucerne (Medicago sativa L. complex)     

L. Pecetti  E. Piano  M. Romani 《Euphytica》2004,135(1):89-97

Although agronomically interesting, selection of creeping-rootedness inlucerne, that is, the ability to form adventitious shoots on horizontal,spreading roots, remains difficult because of the complex and still unclear geneticcontrol of this character, coupled with the possible occurrence of external factorsthat affect unpredictably its expression. Two experiments were undertaken onprogenies of creeping-rooted plants, to verify previous findings and inferences,and test novel hypotheses, concerning the effect of various plant factors on theexpression of creeping-rootedness. The ultimate aim was to provide information onthe most appropriate germplasm, procedures, or indirect selection criteria, that wouldimprove the efficiency of breeding for this character. The factors here examinedincluded genotype, age, kind of progeny, vigour, spreading ability, and undergroundmorphology. Some genotypes appeared to be good donors of the character, whereasothers did not. The proportion of creeping-rooted plants increased in allcases with the plant age. Selfing consistently depressed the expression ofthe character compared with clonal propagation or crossing. It is suggestedthat the effect of age and selfing on the penetrance of creeping-rootedness ismediated through their effect on plant vigour. Both aerial and root vigour showedindeed an effect per se on the presence of the character. Beside thevigour, possible indirect selection criteria were identified in the frequenciesof swollen zones and latent stem primordia on horizontal roots. Proportion ofcreeping-rooted plants in progenies after one year of growth appeared as a simple anduseful criterion to predict future trends. Consistent with previous conclusions, theresults provided evidence that a combination of family and individualselection may enhance the character expression.  相似文献   

The Biology of the Larger Grain Borer, Prostephanus truncatus (Horn) (Coleoptera: Bostrichidae)     

C. Nansen  W.G. Meikle 《Integrated Pest Management Reviews》2002,7(2):91-104

After about 25 years of intensive research a substantial moment of information has accumulated on the basic biology of Prostephanus truncatus in stored products. This article reviews the literature on the geographical distribution, biotypes, symbiotic associations, mating and flight behaviour, oviposition, and development on both agricultural and non-agricultural hosts. The current knowledge about the nutritional biology (including the role of symbionts) and host finding behaviour (including the inter-linked roles of plant chemicals and the insect's own pheronones) are highlighted as research areas which deserve future attention. In addition, few studies have been conducted to determine the extent to which the biology of P. truncatus permits it to survive and reproduce in non-agricultural environments. These areas of study should be pursued as possible routes to providing more effective integrated pest managements strategies for the larger grain borer.  相似文献   

巴楚蘑菇孢子弹射与收集方法的研究   总被引：1，自引：0，他引：1  

胡建伟  郭楚燕  龚明福  徐金波 《中国食用菌》2003,22(5):15-16

巴楚蘑菇在新疆塔里木盆地极其炎热干燥气候条件下，其孢子与一般子囊菌的一样可向空气中弹射，但主要是以耳片在水中弹射孢子的方式进行孢子释放，孢子随水进行传播。孢子收集时将耳片放入水中诱导耳片释放孢子，可比较容易地收集到无菌孢子。  相似文献   

山羊胎期肺发育的形态学研究     

郑月茂  徐永平  卿素珠  蒲鹏  张涌 《畜牧兽医学报》2005,36(2):158-165

对3～22周龄山羊胎儿肺进行了肉眼、光镜和透射电镜观察，结果表明：1．7～22周龄山羊胎儿肺的外部形态与胎龄无关，肺的外部形态以左二右四叶者为多见．肺的叶间裂和右肺副裂常不完整，以浆膜、肺组织或混合性组织(肺组织及浆膜)融合；2．山羊胎儿肺的发育分为5个时期：胚胎期(3～5周)肺芽分支形成主支气管，主支气管长度不断增长并萌芽出叶支气管，均衬以假复层柱状上皮。腺状期(6～12周)以支气管树发育为主，小支气管衬以假复层和／或单层柱状上皮；终蕾呈腺状，上皮细胞由假复层柱状逐渐变为单层柱状，胞核向细胞顶端移行；终蕾上皮细胞游离面可见短小的微绒毛；线粒体、粗面内质网及核糖体随着胎龄增加而逐渐增多，它们均位于细胞顶部。小管期(13～14周)以呼吸部发育为主，原始肺泡开始形成，呼吸性细支气管衬以未分化的立方上皮；终蕾腺状结构逐渐消失，终蕾上皮细胞由高矮不等的单层柱状上皮逐渐演变为立方形的原始肺泡上皮；细胞游离面可见较多的微绒毛，胞质内线粒体、粗面内质网及核糖体较发达。囊状期(第15周)呼吸部发育显著，肺内细支气管及其末端呈现出“充气”状态；部分原始肺泡上皮细胞分化为扁平的肺泡Ⅰ型细胞和立方形的肺泡Ⅱ型细胞；Ⅱ型细胞内出现嗜锇小体。肺泡期(16～22周)以肺泡的形成和分化为主，更多的肺泡上皮分化为扁平的肺泡Ⅰ型细胞和立方形的肺泡Ⅱ型细胞。此期，毛细血管内皮与部分肺泡上皮贴近，可将肺泡上皮细胞区分为3种：Ⅰ型细胞，呈矮柱状或椭圆形，胞质中有较明显的核糖体、扩张内质网及变性线粒体；形成了由Ⅰ型细胞一基膜一内皮细胞组成的气血屏障。Ⅱ型细胞，胞质内含丰富的嗜锇板层小体和核糖体，内质网扩张呈大小不一的泡状，多泡体出现，线粒体膨大变性，细胞游离面可见少数微绒毛。Ⅲ型细胞，为未分化细胞，呈立方形，胞体较小，胞核相对较大，呈圆或椭圆形，胞质少，呈带状．电子密度低，细胞器少。  相似文献   

Morphofunctional description of mucous cells in the gills of the Arapaimidae Arapaima gigas (Cuvier) during its development          下载免费PDF全文

C. A. Ramos  O. T. F. da Costa  W. L. P. Duncan  M. N. Fernandes 《Anatomia, histologia, embryologia》2018,47(4):330-337

The gill structure of the Amazonian fish Arapaima gigas (Cuvier 1829) shows ontogenetic changes during development, particularly due the transition from the aquatic to the obligatory air breathing mode of respiration. However, three main cell types can be found in the gills: mitochondrial rich cells, pavement cells and mucous cells (MCs). The MCs are involved in the secretory pathway. The functions of the secreted molecules include mechanical protection of epithelia, protection against parasites and bacterial infection, and role on ion regulation. In this study, we analysed mucous cell location and mucous cell type, based on pH, during the development of A. gigas. Using samples obtained from the environment, gills were collected and fixed in buffered solution. Histological techniques for the identification of MCs were performed Alcian Blue (AB) and periodic acid‐Schiff (PAS). The results showed the presence of PAS+ and AB+ cells in the whole filament in all examined fish. In animals less than 50 g, few MCs were present, and no differences were observed in AB+ and PAS+ cells. In animals weighing close to 500 g, more PAS+ cells than AB+ cells were observed, and in animals that weighed more than 1,000 g, more AB+ cells than PAS+ cells were observed. These observations may be a result of the ontogenetic changes in the gill epithelia, which can change the osmorespiratory compromise in ion regulation functions as well the glycosaminoglycans secreted by PAS cells, which in large animals can play a role in the protection against parasites and bacterial infection.  相似文献