杨梅属植物共生结瘤固氮研究进展   总被引：2，自引：0，他引：2  

何新华  陈力耕  胡西琴 《果树学报》2002,19(5):351-355

杨梅属35个种中有28个种能与Frankia放线菌共生结瘤固氮。根据有关文献从杨梅属植物共生固氮根瘤及其内生放线菌的形态结构、杨梅属植物根瘤内生放线菌的分类地位、杨梅属植物共生固氮放线菌的生理生化特性、环境条件对杨梅属植物根瘤共生固氮的影响以及分子生物学方法在杨梅属植物根瘤共生固氮放线菌中的应用等方面作了较为详细的阐述。  相似文献   

纤维粗度对浆张性能的影响   总被引：3，自引：0，他引：3  

洪传真 《林产工业》1997,(6)

利用纤维质量分析仪研究针、阔叶木浆打浆后的纤维形态，观察打浆后的纤维粗度及测定手抄片的性能。结果表明，打浆能改变纤维的粗度，从而影响手抄片的物理性能和不透明度。  相似文献   

The combined effects of cover crops and symbiotic microbes on phosphatase gene and organic phosphorus hydrolysis in subtropical orchard soils     

《Soil biology & biochemistry》2015

P deficiency is a major obstacle for crop production in subtropical red soils in South China, and the hydrolysis of organic P (Po) is of great significance in these soils due to the immobilization of P by Fe and Al. Cover cropping in orchards and symbiotic microbial inoculation are considered to improve soil quality, including P status, however, their effects on the hydrolysis of Po is little known. In this study, five soil managements were established in a guava orchard in South China for two and a half years, including clean culture (CC), cover cropping with Paspalum natatu (PN), PN with arbuscular mycorrhizal fungal inoculation (PNA), cover cropping with Stylosanthes guianensis (SG), SG with rhizobial inoculation (SGR). Soil chemical, biochemical and microbial properties were analyzed. Results indicate that soil pH and SOM content tended to increase following cover cropping alone or with microbial inoculation. Po content was significantly elevated in PNA. Po fractionation revealed that cover cropping alone or with microbial inoculation significantly affected the contents of moderately labile Po (MLPo) and moderately resistant Po (FAPo). Enzyme assay indicated that cover cropping with microbial inoculation increased the activities of acidic phosphomonoesterase (ACP), neutral phosphomonoesterase (NP) and alkaline phosphomonoesterase (ALP), with ALP the most sensitive, although ACP activity dominated in red soils. Correlation analysis suggested a significantly positive relationship between ALP activity and MLPo or FAPo. PCR-DGGE profile of the alp-harboring bacterial community showed that cover cropping with S. guianensis and mycorrhizal inoculation to P. natatu promoted the bacterial diversity and/or species richness. For almost all the measured parameters, PN and SG were comparable, however, PNA was superior to SGR, indicating the stronger additive effect of arbuscular mycorrhizal fungus than that of rhizobia. Cat-PCA indicated that MLPo was the most influential factor on phosphomonoesterase. In general, this study suggests that, in subtropical orchards with red soil, cover cropping with microbial inoculation can improve the Po hydrolysis via the promoted alp-harboring bacterial community and then ALP activity. Our results also suggest that the combination of P. natatu and arbuscular mycorrhizal fungus is better than S. guianensis and rhizobia, which possesses practical significance for sustainable production in these orchards.  相似文献   

基于渐进形态学开运算和偏度平衡法的LiDAR数据滤波方法     

林金彦  邹时林  胡永杰 《安徽农业科学》2015,(16):351-354

基于高程的偏度参数统计的LiDAR数据滤波对地形起伏城区滤波适应性不强的弱点,对该方法进行了改进,提出了结合渐进形态学开运算和偏度平衡法的LiDAR数据滤波方法,该方法的原理就是将所有引起高程的突变转换成全由植被、建筑等非地面点引起,而不是由地形高程引起的,这个转换的中间量就是高差,即将高差作为偏度系数的统计对象.并通过实验验证了该方法的合理性,实验结果表明,改进的方法不仅延续了参数统计法无需阈值的优势,而且对城区滤波更具适应性、合理性和稳定性,能够明显地提高滤波精度.  相似文献   

城区道路景观共生模式研究——以金寨县将军大道为例     

许伟 《安徽农业科学》2011,39(24):14845-14848

对小城镇沿河主干道的道路周边用地和道路中央分隔带进行景观设计和植物配置,将生态学中的共生机制原理运用到景观规划中,将地形、地貌、地物的自然景观,满足人类精神需要的人文景观,道路行车安全的功能景观和谐优化,初步探讨出一种共生机制下的小城镇道路景观共生模式。  相似文献   

PWM变量喷雾系统动态雾滴分布均匀性实验   总被引：4，自引：0，他引：4  

蒋焕煜  周鸣川  李华融  蒋卓华 《农业机械学报》2015,46(3):73-77

由于PWM变量喷雾作业过程中喷头不连续作业,喷雾的均匀性特别是喷雾机运动方向上的均匀性较难控制,为此通过高速电磁阀、不锈钢压力罐、压力传感器、气泵、调速输送带等构建了一套动态PWM变量喷雾实验平台,并对该平台动态喷雾雾滴分布特性进行实验研究。采用水敏试纸作为获取动态雾滴分布状态手段,通过图像处理技术以区域内雾滴覆盖率的变异系数作为动态雾滴分布均匀性判定指标,评估了在不同PWM控制信号频率、不同PWM控制信号占空比及不同喷雾压力下的单个喷头动态雾滴分布均匀程度。经实验表明,变异系数随控制信号占空比的增大而减小,控制信号频率对动态喷雾雾滴分布均匀性有较大影响,变异系数随控制信号频率增大而减小,喷雾压力对变异系数影响较小,喷雾压力越大变异系数越大。  相似文献   

Involvement of Bradyrhizobium japonicum denitrification in symbiotic nitrogen fixation by soybean plants subjected to flooding     

Cristina Sánchez  Arsenio Granados  Eulogio J. Bedmar 《Soil biology & biochemistry》2011,43(1):212-217

Denitrification by Bradyrhizobium japonicum bacteroids contributes to nitric oxide (NO) production within soybean nodules in response to flooding conditions. However, the physiological relevance of NO production by denitrification in B. japonicum-Glycine max symbiosis is still unclear. In this work, soybean plants were inoculated with B. japonicum strains lacking the nirK or norC genes which encode the copper-containing nitrite reductase and the c-type nitric oxide reductase enzymes, respectively. 14 days flooding increased nodule number of plants inoculated with the WT and norC strains, but not of plants inoculated with the nirK mutant. However, nodule dry weight was not affected by 14 days flooding regardless of the strain used for inoculation. Supporting this observation, individual nodule growth was significantly higher in plants inoculated with nirK than those inoculated with WT or norC after 14 days flooding. Nodule functioning was strongly inhibited by flooding since leghemoglobin content of the nodules induced by any of the strains was significantly decreased after 7 or 14 days flooding compared to control plants. However, this effect was more relevant in nodules of plants inoculated with the WT or norC mutant than in those inoculated with the nirK mutant. Nitrogen fixation was also estimated by analyzing nitrogen content derived from biological nitrogen fixation in shoots, using the ¹⁵N isotope dilution technique. By using this approach, we observed that the negative effect of 14 days flooding on nitrogen fixation was more pronounced in plants inoculated with the norC mutant. However, nitrogen fixation of plants inoculated with nirK showed the highest tolerance to 14 days flooding. These findings allowed us to demonstrate the previously proposed hypothesis which suggests that NO formed by copper-containing nitrite reductase in soybean nodules, in response to flooding, has a negative effect on nitrogenase activity. We propose that inoculation of soybeans with a B. japonicum nirK mutant, which does not produce NO from nitrate, increases the tolerance of symbiotic nitrogen fixation to flooding.  相似文献   

8株优良大豆根瘤菌与不同地区27个大豆主栽品种的匹配性研究   总被引：2，自引：0，他引：2  

伍惠  钟喆栋  樊伟  彭亚齐  杜思  陈大松  王学路  李友国 《大豆科学》2017,36(3)

大豆是我国重要的油料作物和高蛋白粮饲兼用作物,具有与根瘤菌共生固氮的能力。近十几年来,我国大豆品种更新快,导致大豆根瘤菌株与新品种匹配能力差、接种效果不明显。筛选与主栽大豆品种匹配性好、固氮效率高的广谱性优良菌株,可为针对性的施用大豆根瘤菌接种剂提供菌种资源和方案。选取本实验室前期分离保存的6个优良快生型大豆根瘤菌株和2个慢生型大豆根瘤菌株,在砂培盆栽条件下与不同地区的27个大豆主栽品种进行匹配试验。测定分析了植株地上部分生物量、高度、根瘤数量、根瘤生物量和根瘤固氮酶活指标。结果表明:不同大豆根瘤菌之间结瘤固氮能力存在极显著的差异;供试根瘤菌均能够与国内24个大豆品种结瘤,广谱性较好;植株地上部分高度、根瘤数量、根瘤生物量和根瘤固氮酶活与地上部分生物量呈显著相关;大部分接种根瘤菌后的植物生物量显著高于CK;HN01、GR3、HH29、HH103匹配性和固氮效率均不逊色于USDA110,具有在东北地区、黄淮海地区、长江流域、东南地区推广的潜能;从品种来看,中豆39、BD2、天隆1号与8株供试大豆根瘤菌的匹配接种表现出高生物量特点。此外,本文还筛选出大豆-大豆根瘤菌的表型最佳匹配组合中豆39-GR3,适合长江流域地区;同样筛选到东北地区、黄淮海地区、东南地区最佳匹配组合,分别是HN01-辽豆14、HN01-徐豆14、HN01-BD2。本文初步建立了优良根瘤菌与大豆主栽品种的匹配关系,为在田间试验中进一步筛选和应用这些优良菌株提供了材料和指导。  相似文献   

覆膜滴灌条件下棉花根层土壤盐分时间稳定性研究   总被引：3，自引：0，他引：3  

邢旭光  赵文刚  马孝义  赵伟  史文娟 《农业机械学报》2015,46(7):146-153

为揭示膜下滴灌棉田主根区土壤盐分的时间变异特性,基于2013—2014年田间实测数据,采用变异系数、平均相对偏差和标准差等方法研究了不同土层土壤盐分的时间稳定性,并进一步确定了可以反映各土层土壤平均含盐量的代表性测点。结果表明,研究区域0~40 cm土层土壤盐分随时间序列的变异性只有少数几个测点属于强变异,其余均属于中等变异;在棉花主根层(40 cm)内,土壤盐分的时间稳定性随土层深度增加呈现先增强后略微减弱趋势,在30 cm土层深度稳定性最强,平均相对偏差浮动范围最小、且其平均标准差最小;反映各土层平均含盐量的代表测点分布较为集中,可选取代表地块对区域土壤含盐量进行估算(决定系数R2为0.791 2~0.917 1)。棉田主根层土壤盐分时间稳定性研究有利于指导田间灌溉;选取少量且具有代表性的测点可为区域合理布设土壤盐分监测点提供理论依据。  相似文献   

A yield-independent, 15N-isotope dilution method to estimate legume symbiotic dependence without a non-N2-fixing reference plant   总被引：1，自引：0，他引：1  

P. M. Chalk  C. J. Smith  P. Hopmans  S. D. Hamilton 《Biology and Fertility of Soils》1996,23(2):196-199

The proportional contribution of atmospheric N₂ to the N nutrition of lupin (P _atm) was estimated in a field experiment following addition of NH₄Cl of KNO₃ to unconfined microplots (1.5 m²) at 2.5 g N m^-2 (10 atom% ¹⁵N). The integrated ¹⁵N enrichment, or mean pool abundance, of nitrate extracted from 0- to 15-cm samples taken under the lupin crop on eight occasion between 28 and 190 days after sowing was used as the reference criterion to estimate P _atm by the ¹⁵N-isotope dilution technique. Estimates of P _atm were similar to those obtained using canola as a non-fixing reference plant, but were higher than estimates obtained using a yield-dependent model. Use of mean pool abundance obviates the need for a non-fixing reference plant, and the frequent sampling and isotope-ratio analysis of the legume biomass required with the yield-dependent model is unnecessary. However, further work is needed to validate a sampling strategy commensurate with the growth of the legume roots.  相似文献