藏绵羊卵巢组织学及卵泡超微形态的观察     

郭亚军  柳苗苗  付德海  冉兴荣  王欣荣 《畜牧兽医学报》2021,52(2):389-398

旨在通过观察藏绵羊卵泡、黄体的组织学特征及卵泡的超微形态,探讨其与生理功能的关系。本研究运用大体解剖、常规组织切片和H.E染色及透射电镜技术对藏绵羊卵巢卵泡和黄体的组织结构特点以及卵泡的超微形态进行观察和分析。结果发现,藏绵羊黄体期和卵泡期卵巢的宽度和厚度存在显著差异（P<0.05）,而重量和长度无显著差异（P>0.05）;卵巢上的大多数卵泡通过颗粒细胞萎缩和纤维化而闭锁;黄体中膜黄体细胞（直径22 μm）和颗粒黄体细胞（直径50 μm）结构特征明显且分界清晰,其内部分布着丰富的毛细血管;通过观察卵泡的超微形态发现,随着卵泡的发育,卵泡细胞的形状由椭圆形变为多边形,数量增多且被膜细胞包围;细胞器数量和种类也增多且之间联系紧密,胞质可见分泌的皮质颗粒。结果表明,藏绵羊卵泡系统和黄体的组织学特点以及卵泡的超微形态与其他绵、山羊类似,随着卵泡的发育,血管增生较快,卵泡结构特征变化明显和细胞器致密化程度增加且卵泡在不同时期均会闭锁;黄体内部在毛细血管滋生下使两种黄体细胞结构更加完整且特征明显,这可能是藏绵羊在高原低氧环境下仍然能够发挥卵巢机能的重要基础保障。  相似文献   

Ultrasonographic findings of the corpus luteum and the gravid uterus during heat stress in dairy cattle     

Ioannis Nanas  Mariana Barbagianni  Katerina Dadouli  Eleni Dovolou  Georgios S Amiridis 《Reproduction in domestic animals》2021,56(10):1329-1341

The objectives of this study were to assess alterations in, echogenic appearance, size and blood flow in the corpus luteum, the placentomes and the blood flow in umbilical and uterine arteries that heat stress can cause in cooled pregnant dairy cows. Pregnant cows were allocated in two groups and the gravid uteri, along with the ipsilateral corpora lutea were examined during the winter (group W, n = 9) or the summer (group S, n = 10). The grey-scale ultrasound and colour flow imaging of the corpus luteum and placentome were performed. In addition, the umbilical and uterine artery diameters and haemodynamic parameters in the vessels were calculated. At the time of ultrasonographic examination, cortisol concentrations were higher, and progesterone levels tended to be lower in group S compared to group W. The grey-scale ultrasound evaluation of corpora lutea and placentomes was lower in group S compared to group W. The diameter of umbilical artery and the blood volume in the vessel were less in group S than in group W. We infer that heat stress affects foetal blood supply and possibly the structure of placentomes and corpora lutea, but it differently affects the blood flow characteristics in the umbilical and uterine arteries.  相似文献   

Accessory corpora lutea formation in pregnant Hokkaido sika deer (Cervus nippon yesoensis) investigated by examination of ovarian dynamics and steroid hormone concentrations     

Yojiro YANAGAWA  Yukiko MATSUURA  Masatsugu SUZUKI  Shin-ichi SAGA  Hideto OKUYAMA  Daisuke FUKUI  Gen BANDO  Masashi NAGANO  Seiji KATAGIRI  Yoshiyuki TAKAHASHI  Toshio TSUBOTA 《The Journal of reproduction and development》2015,61(1):61-66

Generally, sika deer conceive a single fetus, but approximately 80% of pregnant females have two corpora lutea (CLs). The function of the accessory CL (ACL) is unknown; moreover, the process of ACL formation is unclear, and understanding this is necessary to know its role. To elucidate the process of ACL formation, the ovarian dynamics of six adult Hokkaido sika deer females were examined ultrasonographically together with peripheral estradiol-17β and progesterone concentrations. ACLs formed in three females that conceived at the first estrus of the breeding season, but not in those females that conceived at the second estrus. After copulation, postconception ovulation of the dominant follicle of the first wave is induced by an increase in estradiol-17β, which leads to formation of an ACL. A relatively low concentration of progesterone after the first estrus of the breeding season is considered to be responsible for the increase in estradiol-17β after copulation.  相似文献   

A Corpus-based Study on Collocation and Colligation of ＂Soil＂ in Agricultural English     

Hu Jia-ying Sun Yuan-yuan 《东北农业大学学报(英文版)》2014,21(4):86-92

This paper was based on the corpus built by the writers on agricultural science and technology English. Through corpusbased research and statistical tools Ant Conc 3.2.1 and BFSU Collocator 1.0（Beijing Foreign Studies University Collocator 1.0）, this paper tried to make an analysis of the collocation and colligation of the word ＂soil＂. The result revealed the colligation between ＂soil＂ and its collocates in agricultural academic papers, which was instructive to academic writing and the research method was a practical reference of building a corpus on agricultural science and technology English.  相似文献   

牛卵巢黄体类型与卵泡生长发育关系的组织学研究   总被引：4，自引：0，他引：4  

高建明  吴学清  高立云  李华  胡平  林涛 《北京农学院学报》2001,16(2):45-49

本试验通过对牛卵巢不同黄体类型和各级卵泡生长发育状况的组织学观察 ,探讨了不同黄体类型对各级卵泡生长发育的影响 试验结果表明 :1)不同类型黄体之间 ,黄体功能的强弱不同 黄体功能由强至弱依次为圆锥状型 (Ⅰ型 )、火山口状型 (Ⅱ型 )、蘑菇状型 (Ⅲ型 )、扁平片状型 (Ⅳ型 )、表面无黄体型 (Ⅴ型 ) 2 )黄体类型对生长发育过程中各级卵泡的数量有一定的影响 Ⅴ型腔前卵泡总数和有腔卵泡总数与Ⅰ、Ⅱ、Ⅲ、Ⅳ型的腔前卵泡总数和有腔卵泡总数之间差异极显著 (p <0 0 1) ,而Ⅰ、Ⅱ型之间差异不显著 Ⅲ、Ⅳ之间差异显著 ,Ⅰ、Ⅱ型与Ⅲ、Ⅳ型之间差异显著 (p <0 0 5) 3)黄体类型对各级卵泡平均直径均无显著影响  相似文献   

Immunocytochemical Localization of Aromatase in The Ovary of Superovulated Cattle,Pigs and Sheep     

J. Lautincik  L. Kolodzieyski  V. Elias  P. Hyttel  Y. Osawa  A. Sirotkin 《Acta veterinaria Scandinavica》1994,35(2):185

Laurincik, J., L. Kolodzieyski, V. Elias, P. Hyttel, Y. Osawa, A. Sirotkin: Immunocytochemical localization of aromatase in the ovary of superovulated cattle, pigs and sheep. Acta vet. scand. 1994, 35, 185-191.–The localization of aromatase, an enzyme converting androgens to estrogen, in the ovaries of superovulated cattle, pigs and sheep was studied immunocytochemically in the preovulatory and postovulatory period using anti-human placental aromatase cytochrome P-450 antiserum. Immunostaining for aromatase was detected in the granulosa cells of preovulatory follicles of all species studied. Theca interna cells were stained in preovulatory follicles in the pig but not in cattle and sheep. Interstitial gland cells, cumulus cells and oocytes were unstained in all species. In cattle and pig the corpora lutea were unstained whereas they displayed staining in the sheep. Preantral and small antral follicles were unstained during both the preovulatory and postovulatory period in all species.It is concluded that granulosa cells of preovulatory follicles are the main residence for aromatase activity in superovulated cattle, pig and sheep, whereas the activity of theca interna and corpora lutea is species specific.  相似文献   

抑制素基因免疫黄牛的黄体发育与免疫纯度及剂量的关系     

姜卫星  刘友生  薛立群  杨利国  王水莲 《湖南农业大学学报(自然科学版)》2012,38(3):300-304

为探讨双拷贝抑制素pcISI基因疫苗纯度和剂量对肉牛黄体发育的影响,将150头同期发情的南阳黄牛随机分为8个试验组和2个对照组,试验组用pcISI按2种纯度、4种剂量(0.75、1.50、2.25、3.00 mg/头)进行免疫处理,2个对照组分别用空质粒和生理盐水进行处理;初次免疫21 d后进行加强免疫。结果表明:无论用高纯度还是低纯度疫苗免疫,2.25 mg/头剂量处理对黄体的作用效果最明显,且抗体水平与黄体发育密切相关,2.25 mg/头抑制素融合表达质粒组的黄体显著大于0.75 mg/头组(P<0.05),抗体阳性牛两侧黄体显著比抗体阴性牛的大(P<0.05)。  相似文献   

Effect of oxytocin infusion on luteal blood flow and progesterone secretion in dairy cattle     

Christos N. Brozos  Metin S. Pancarci  Javier Valencia  Nikola Beindorff  Georgios Tsousis  Evaggelos Kiossis  Heinrich Bollwein 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(1):67-71

The objective of this study was to investigate the effects of oxytocin infusion on corpus luteum (CL) function during early to mid-diestrus by measuring luteal size (LS) and luteal blood flow (LBF) along with plasma levels of progesterone (P4) and prostaglandin metabolites (13,14-dihydro-15-keto-prostaglandin F_2α, PGFM). On day (D) 7 of the estrus cycle (D1 = ovulation), seven cows received 100 IU of oxytocin (OXY) or placebo (PL) following a Latin square design. LS and LBF increased in both groups over time and no differences were observed between the groups. PGFM did not differ either within the groups over time or between the groups at any time point. P4 of the OXY group was higher compared to that of the the PL group 360 min after the infusion (p = 0.01) and tended to be higher at the time points 450 min, 48 h, and 72 h (all p = 0.08). Results from this study support the hypothesis that OXY is not directly involved in the mechanism(s) governing blood flow of the CL and has no remarkable effects either on luteal size or P4 and PGFM plasma levels. Further investigation is needed to elucidate the role of OXY in CL blood flow during early and late luteal phases.  相似文献   

Effects of ovarian structures identified at Ovsynch™ enrolment,disease history and lactation variables on odds of pregnancy to a fixed‐time artificial insemination after Ovsynch™          下载免费PDF全文

《Australian veterinary journal》2017,95(4):110-117

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Effect of chronic administration of a gonadotropin‐releasing agonist on luteal function and pregnancy rates in dairy cattle     

Alan Willmore  Tracy L. Davis 《Animal Science Journal》2019,90(11):1432-1443

Increased embryonic losses may be associated with inadequate progesterone (P4) concentrations in high‐producing lactating dairy cattle. The objectives of the present studies were to determine if chronic administration of a gonadotropin‐releasing hormone (GnRH) agonist, Deslorelin, would increase circulating P4 concentrations and subsequently increase pregnancy rates in dairy cattle. Administration of Deslorelin for 12 days increased (p < .05) luteal volume and circulating P4 concentrations in primiparous lactating dairy cows, but increased only luteal volumes in multiparous cows. Treatment with Deslorelin increased Day 45 pregnancy rates in cows as compared to untreated controls. Chronic treatment with Deslorelin in dairy cattle; (a) increased luteal volume of the primary CL, (b) induced accessory CL, (c) increased circulating P4 concentration in primiparous cows only, (d) did not lengthen the estrous cycle upon removal of treatment, and (e) increased pregnancy rates. Although luteal volume was increased in multiparous cows and circulating P4 concentrations were not with Deslorelin treatment, there was an apparent effect on pregnancy rates. This hormonal strategy may represent a suitable model to address local effects of P4 and GnRH/luteinizing hormone on uterine environment and subsequent embryonic survival.  相似文献