排序方式： 共有14条查询结果，搜索用时 15 毫秒
1.
2.
3.
4.
The recent development of genome editing technologies has given researchers unprecedented power to alter DNA sequences at chosen genomic loci, thereby generating various genetically edited animal models. This mini-review briefly summarizes the development of major genome editing tools, focusing on the application of these tools to generate animal models in multiple species. 相似文献
5.
Wataru FUJII Asuka ONUMA Shin YOSHIOKA Keisuke NAGASHIMA Koji SUGIURA Kunihiko NAITO 《The Journal of reproduction and development》2015,61(6):589-593
The generation efficiencies of mutation-induced mice when using engineered zinc-finger nucleases (ZFNs) have
been generally 10 to 20% of obtained pups in previous studies. The discovery of high-affinity DNA-binding
modules can contribute to the generation of various kinds of novel artificial chromatin-targeting tools, such
as zinc-finger acetyltransferases, zinc-finger histone kinases and so on, as well as improvement of reported
zinc-finger recombinases and zinc-finger methyltransferases. Here, we report a novel ZFN pair that has a
highly efficient mutation-induction ability in murine zygotes. The ZFN pair induced mutations in all obtained
mice in the target locus, exon 17 of aminopeptidase Q gene, and almost all of the pups had biallelic
mutations. This high efficiency was also shown in the plasmid DNA transfected in a cultured human cell line.
The induced mutations were inherited normally in the next generation. The zinc-finger modules of this ZFN pair
are expected to contribute to the development of novel ZF-attached chromatin-targeting tools. 相似文献
6.
7.
Global population will increase to over nine billion by 2050 with the doubling in demand for meat and milk. To overcome this challenge, it is necessary to breed highly efficient and productive livestock. Furthermore, livestock are also excellent models for human diseases and ideal bioreactors to produce pharmaceutical proteins. Thus, genetic engineering of domestic animals presents a critical and valuable tool to address these agricultural and biomedical applications. Overall, genetic engineering has evolved through three stages in history: transgenesis, gene targeting, and gene editing. Since the birth of the first transgenic pig, genetic engineering in livestock has been advancing slowly due to inherent technical limitations. A major breakthrough has been the advent of somatic cell nuclear transfer, which, for the first time, provided the technical ability to produce site-specific genome-modified domestic animals. However, the low efficiency of gene targeting events in somatic cells prohibits its wide use in agricultural and biomedical applications. Recently, rapid progress in tools and methods of genome engineering has been made, allowing genetic editing from mutation of a single base pair to the deletion of entire chromosomes. Here, we review the major advances of genetic engineering in domestic animals with emphasis placed on the introduction of latest designer nucleases. 相似文献
8.
9.
《畜牧与兽医》2015,(7):124-129
随着人类测序技术的发展与成熟,以锌指核酸酶(ZFN)、类转录激活因子效应物核酸酶(TALEN)以及成簇的规律间隔的短回文重复序列及相关基因(CRISPR/Cas)为主的基因组编辑技术,在生物、农业、环境及医学等各个领域中发挥着独特的作用。基因编辑技术主要是通过在外源DNA靶位点上产生双链切口,从而诱导出同源重组修复或非同源末端连接,进而实现基因组的编辑。目前ZFN和TALEN技术被广泛运用,虽然在一定程度上获得明显的效果,但因设计复杂,成本较高,使其运用有所局限。CRISPR/Cas技术近年来凭借其高效、精确的编辑成为最新一代的基因编辑技术。本文就以上3种基因打靶技术的作用原理、应用及优缺点作简单概述,为基因编辑的实践应用提供理论基础。 相似文献
10.