改良背主动脉注射法对鸡胚发育与转基因表达效率的影响     

韦金鱼  韦茏芹  徐小明  邢青波  肖艳宇  许梦缘  吴文德  李恭贺  郑喜邦 《中国畜牧杂志》2021,(2):191-195

背主动脉注射是生产转基因鸡的经典方法,该方法不需换壳培养,但壳内注射技术难度大,并且无法实时观察后期胚胎发育情况。本实验对背主动脉注射法进行了改进,将增强型绿色荧光蛋白(EGFP)腺相关病毒(Adeno-associated Virus,AAV)壳外注射至150枚HH 14~16期(Hamburger-Hamilton Stage 14~16)鸡胚背主动脉中,再进行换壳培养,继续孵化至出壳,观察和分析改良背主动脉注射法与传统背主动脉注射法和胚盘下腔注射法对发育8、14、18、21 d鸡胚存活率、孵化率以及EGFP阳性检出率的影响。结果表明:改良背主动脉注射组鸡胚存活率均高于传统背主动脉注射组与胚盘下腔注射组(P<0.05或P<0.01);改良背主动脉注射组的鸡胚孵化率(37%)高于传统背主动脉注射组(16%)与胚盘下腔注射组(28%)(P<0.01);荧光蛋白手电筒检测显示,改良背主动脉注射组鸡胚EGFP阳性率(17%)明显高于传统背主动脉注射组(13%)与胚盘下腔注射组(12%)(P<0.01)。综上,背主动脉壳外注射结合换壳培养提高了转基因鸡胚胎孵化率和EGFP阳性检出率,对提高转基因鸡效率具有重要价值。  相似文献   

Ultrasound-guided modified subcostal transversus abdominis plane block and influence of recumbency position on dye spread in equine cadavers     

Flavio A.V. Freitag  Dorli da S. Amora  Eloisa Muehlbauer  Peterson T. Dornbusch  Marcello Machado  Fabiano Montiani-Ferreira  Edison L. Prisco Farias  Alexander Valverde  Juan Carlos Duque Moreno 《Veterinary anaesthesia and analgesia》2021,48(4):596-602

ObjectiveTo describe and evaluate an ultrasound-guided modified subcostal approach for the transversus abdominis plane (TAP) block in horse cadavers in lateral or dorsal recumbency.Study designProspective, experimental cadaveric study.AnimalsStudy of one preserved foal and eight fresh adult horse cadavers.MethodsThe lateral and ventral abdominal wall of a preserved cadaver was dissected to identify the muscles and nerves. A unilateral standard TAP block technique was performed (60 mL of methylene blue dye–bupivacaine) on a fresh cadaver in right lateral recumbency. A modified subcostal technique was performed on the opposite side using a linear ultrasound transducer and in-plane approach. Injection points (two 30 mL dye) were at the level of the TAP (between the rectus abdominis and transversus abdominis muscles and ventral to the cutaneous trunci muscle) perpendicular to: 1) the mid-point between the xiphoid cartilage and umbilical scar; and 2) at a point between the caudal and middle thirds of the abdomen measured from the first injection point to the umbilical scar. The modified subcostal approach was performed in seven additional cadavers in both hemiabdomens, with three cadavers in lateral and four cadavers in dorsal recumbency. Ultrasound guidance was used with all injections.ResultsThe standard approach stained the sixteenth to eighteenth thoracic nerves (T16–T18). The modified subcostal approach performed in lateral recumbency provided greater spread (T9–T17) than dorsal recumbency (T12–T18) (p = 0.016).Conclusions and clinical relevanceThe modified subcostal TAP approach resulted in extensive staining exceeding the standard approach. The nerves stained are consistent with production of ventral abdominal wall anesthesia in horses. Clinical studies are needed to verify these findings.  相似文献   

Surgical Approaches to the Nasal Cavity and Sinuses     

《Veterinary Clinics of North America: Small Animal Practice》2016,46(4):719-733

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Early herniorrhaphy of large traumatic abdominal wounds in horses and mules          下载免费PDF全文

S. Azizi  S.‐M. Hashemi‐Asl  E. Torabi 《Equine veterinary journal》2016,48(4):434-437

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Evaluation of hemostatic derangements associated with canine anaphylaxis and the relationship to syndrome severity     

M. Ryan Smith DVM  DACVECC  Virginie A. Wurlod MS  Dr vet med  DACVECC  DECVECC  Chin-Chi Liu MS  MApStat  PhD 《Journal of Veterinary Emergency and Critical Care》2023,33(6):648-655

Objective

To describe hemostatic derangements associated with canine anaphylaxis and to assess for association with syndrome severity.

Design

Prospective observational study.

Setting

University teaching hospital.

Animals

Twenty-seven client-owned dogs, recruited from November 2018 to January 2022, diagnosed with anaphylaxis of varying severity were included. Study inclusion required presentation <6 hours after initiation of clinical signs, no medications or history of illness within the prior 2 weeks, lack of comorbidities expected to affect hemostasis, and lack of a disease state that could alternatively explain the clinical presentation.

Interventions

Blood samples were collected within the first hour of presentation for CBC, serum biochemistry, prothrombin time (PT), activated partial thromboplastin time (aPTT), and viscoelastic coagulation testing for use with a cartridge-based point-of-care device.

Measurements and main results

Clotting time and clot formation time were prolonged, alpha angle and maximum clot firmness were decreased, PT and aPTT were prolonged, and platelet counts were lower in severe cases compared to mild and moderate cases. There were no differences for any parameter between mild and moderate cases. The presence or absence of abdominal effusion was not associated with hemostatic status.

Conclusions

Global hemostatic derangements consistent with hypocoagulability are a prominent feature of severe anaphylaxis in dogs and should be considered for routine evaluation.  相似文献   

System Design for Leather Cavity XCT     

ZHANG Ping  JIANG Yang 《保鲜与加工》2001,(2):53-56

This paper introduces a method of solving the problem of leather cavity measurement in shoe CAD/CAM by industrial CT technology. Concentrating on peculiarity and requirement of leather cavity measurement, this paper presents a X-ray industrial CT system scheme, which not only satisfy the requirement of leather cavity measurement but also can achieve general industrial CT. measurement. The achievement on data collection system and motion control of stepping motor is emphasized.  相似文献   

Penetration behavior of bath induced by top-blown gas jets for steelmaking converter     

LI Mingming  LI Qiang  ZHANG Wei  ZOU Zongshu 《保鲜与加工》2015,(2):58-63

The cavity induced by top-blow gas jets impinging on the bath surface is one of most important parameters, which has overwhelming influence on the rate of slag formation and metallurgical performance. Water model experiments are conducted through a 1/10 scaled-down top-blown convertor. Effects of lance height and gas flow rate on penetration behavior are studied, and also the influence mechanism of liquid surface tension on penetration depth is addressed based on the experiments and theory analysis. An energy utilization index (EUI) is defined to reflect how much the impinging kinetic energy of gas jets are transferred into bath for formation of cavity, and an improved model is proposed to predict penetration depth of jets. The results show that penetration depth increases with lowering lance height and increasing gas flow rate, and impact diameter increases with increasing lance height, but is little affected by gas flow rate. Influence of liquid surface tension on penetration depth is enhanced with increasing surface tension and penetration depth. The EUI increases with increasing lance height, and a function relationship of it with lance height is obtained based on the experiments.  相似文献   

Cavity spot of carrots ‐ an association with soil ammonium     

《Communications in Soil Science and Plant Analysis》2012,43(6):621-628

Abstract

Cavity spot incidence on sixteen samples of carrots from four fields was found to be correlated with soil ammonium (r = + .78***) and pH (r = ‐ .63**). The calcium concentration in the carrots related to soil ammonium (r = ‐ .65**), but not to cavity spot incidence. Soil nitrate and ammonium were positively associated with each other (r = + .63**), and their ratio (NH₄/NO₃) was very highly correlated with exchangeable manganese (r = + .93***) suggesting that anaerobicity was present.  相似文献   

不同腔长下调Q激光器输出脉冲特性研究     

罗江华  陈海燕 《长江大学学报》2010,7(4):31-33

从Nd3+:YAG脉冲激光器调Q技术出发,研究了不同腔长条件下电光调Q激光器输出脉冲的宽度、能量和峰值功率特征,得到了不同腔长的调Q激光器输出脉冲特性曲线。研究结果表明,激光器腔长的改变能较好地调节脉冲的输出特性,对脉冲激光器的灵活应用具有一定的指导意义。  相似文献   

Abdominal compartment syndrome in a dog with babesiosis     

Kenneth E. Joubert  BVSc  MMedVet      Patricia A. Oglesby  BVSc    Justine Downie  BVSc    Tania Serfontein  DipVetNur 《Journal of Veterinary Emergency and Critical Care》2007,17(2):184-190

Objective: To describe a case of abdominal compartment syndrome in a dog with babesiosis. Case summary: A 9‐year‐old female Bull Terrier presented with complicated babesiosis. Additional findings including respiratory distress, abdominal distension, and a decrease in urine output associated with an elevated abdominal pressure (23.5–25 cmH₂O) led to a further diagnosis of abdominal compartment syndrome. This case report describes the clinical course of both conditions through resolution. New information provided: Abdominal compartment syndrome is not well described in the veterinary literature, but clinicians should be aware of this potential complication in critically ill patients. The pathophysiology and veterinary literature of abdominal compartment syndrome are reviewed.  相似文献