Expression of Anti-Lipopolysaccharide Factor Isoform 3 in Chlamydomonas reinhardtii Showing High Antimicrobial Activity     

Anguo Li  Ruihao Huang  Chaogang Wang  Qunju Hu  Hui Li  Xiao Li 《Marine drugs》2021,19(5)

Antimicrobial peptides are a class of proteins with antibacterial functions. In this study, the anti-lipopolysaccharide factor isoform 3 gene (ALFPm3), encoding an antimicrobial peptide from Penaeus monodon with a super activity was expressed in Chlamydomonas reinhardtii, which would develop a microalga strain that can be used for the antimicrobial peptide production. To construct the expression cluster, namely pH2A-Pm3, the codon optimized ALFPm3 gene was fused with the ble reporter by 2A peptide and inserted into pH124 vector. The glass-bead method was performed to transform pH2A-Pm3 into C. reinhardtii CC-849. In addition to 8 μg/mL zeocin resistance selection, the C. reinhardtii transformants were further confirmed by genomic PCR and RT-PCR. Western blot analysis showed that the C. reinhardtii-derived ALFPm3 (cALFPm3) was successfully expressed in C. reinhardtii transformants and accounted for 0.35% of the total soluble protein (TSP). Furthermore, the results of antibacterial assay revealed that the cALFPm3 could significantly inhibit the growth of a variety of bacteria, including both Gram-negative bacteria and Gram-positive bacteria at a concentration of 0.77 μM. Especially, the inhibition could last longer than 24 h, which performed better than ampicillin. Hence, this study successfully developed a transgenic C. reinhardtii strain, which can produce the active ALFPm3 driven from P. monodon, providing a potential strategy to use C. reinhardtii as the cell factory to produce antimicrobial peptides.  相似文献   

混凝土结构表面蜂窝麻面成因及其防治措施     

吴春华  徐岩峰 《林业科技情报》2009,41(1):56-57

随着建筑业的飞速发展和科技水平的不断提高,工程对混凝土的各种性能要求越来越高,不仅要求混凝土工作性能好、强度指标高、耐久性好等,而且还要求混凝土结构表面无蜂窝麻面光洁如镜的外观,尤其是清水混凝土结构要求更为严格,为此我们必须加以防范,给予重视。  相似文献   

一种新型微生态保健品在兽医临床中的应用研究     

陈铁桥  谢达平 《湖南畜牧兽医》1997,(4)

利用一种微生态保健品和3种抗菌素药物，即氟哌酸、氯霉素、庆大霉素在兽医临床上用于治疗仔猪黄白痢，断奶猪的应激性腹泻，肥猪及母猪的腹泻作了对比试验，结果表明：微生态保健品对仔猪黄痢的治愈率为93％，对仔猪白痢的治愈率为95％，对断奶仔猪的应激性腹泻治愈率为94％，对肥猪及母猪的腹泻治愈率为100％。与氟哌酸、庆大霉素的疗效比较，差异显著（P＜0．05）；与氯霉素比较差异极显著（P＜0．01）．  相似文献   

霉形体膜蛋白溶解及含量测定方法研究   总被引：1，自引：0，他引：1  

张映  张淑改 《山西农业大学学报(自然科学版)》1995,15(3):255-256

本文选择双蒸水；０．１ｍｏｌ／ＬＮａＯＨ；１．０ｍｏｌ／ＬＮａＯＨ；４％ＤＯＣ和１％ＳＤＳ条件分别对猪肺炎霉形体膜制剂作增溶处理，使膜蛋白溶解，然后采用Ｌｏｗｒｙ法进行蛋白质含量的测定．测定结果表明：０．１ｍｏｌ／ＬＮａＯＨ溶解效果较好，所测得膜蛋白含量达６８．８％左右。，数据更为接近实际含量．  相似文献   

国内雏鸭病毒性肝炎的研究进展     

李芙蓉  王永坤 《猪业科学》2003,20(11):62-63

本文就国内雏鸭病毒性肝炎的病原特性、疾病诊断和防制等做一简要综述并提出了该病今后的研究方向。  相似文献   

抗菌肽与抗生素的体外抗菌效果比较   总被引：17，自引：0，他引：17  

汪以真  王中强  许梓荣 《中国兽医学报》2004,24(3):270-273

应用由 Hancock实验室改进的微量肉汤稀释法测定了 cecropin P1、cecropin A、magainin 2、defensin 1、bactenecin、lactoferricin和 indolicidin等 7种抗菌肽和盐酸金霉素、去甲万古霉素、土霉素、强力霉素等 4种抗生素的体外抗菌活性 ,并且使用薄层琼脂糖孔穴扩散法比较了抗菌肽中 cecropin P1和 cecropin A与土霉素和呋喃唑酮对大肠杆菌 K88的抑菌效果。结果发现 ,抗菌肽与抗生素一样对几种革兰氏阳性菌和阴性菌都有不同程度的抗菌效果。其中 cecropin A、cecropin P1和 defensin1对大肠杆菌的 2个菌株 ATCC2 5 92 2和 K88的抗菌活性高于抗生素 ,defensin1是各种抗菌肽中对金黄色葡萄球菌抗菌效果最好的。抗生素对试验用金黄色葡萄球菌的抑制活性总体上要好于抗菌肽。与抗生素相比 ,cecropin A、cecropin P1和 indolicidin对猪霍乱沙门氏菌和鼠伤寒沙门氏菌具有更好的抗菌效果。另外 ,抗菌肽的抑菌圈边缘十分清晰 ,而抗生素的整个抑菌圈都比较模糊且界线不明。因此 ,从抗菌效果方面考虑 ,抗菌肽可以代替抗生素用于疾病的预防和治疗  相似文献   

松香合成中性施胶剂的研究     

丁礼金  程芝 《林产化学与工业》1992,12(4):307-313

  相似文献   

催干剂对氨基环氧树脂阴极电泳漆的影响     

邓光辉  石晓红  蓝虹云  雷福厚 《林产化学与工业》2001,21(2):62-66

以氨基环氧树脂为基体树脂，Co^2 ,Pb^2 盐为催干剂合成了阴极电泳漆，并对膜性能进行考察，结果表明，所合成的阴极电泳涂料在PH值为6－7、电泳时间2min的条件下。加入不同的催干剂，在不同的烘干温度下，得到性能不同的漆膜。经过膝膜综合性能试验，结果表明，加入钴和铅复合催化剂，在160℃左右烘干20min，可得到完全固化，硬度较高、耐潮、耐盐、耐酸碱性较好的漆膜。  相似文献   

西杂牛连续埋植“畜大壮”效果观察   总被引：1，自引：1，他引：0  

赵国琳  李学标 《中国牛业科学》1996,22(1):11-12

本试验对甘肃河西地区西杂１－２岁和周岁以下的犊牛连续用促生长剂“畜大壮”分阶段进行耳根埋植,试验结果表明,４－６月龄犊牛全期１８０天,试验组增重和平均日增重分别为５．５ｋｇ和３０８．３３ｇ,试验组比对照组提高３７．２５ｋｇ和２０６．９４ｇ（Ｐ〈０．０１）,１－２岁牛全期１８０天,试验组平均增重和日增重分别为５６．４３ｋｇ和３１４．４９ｇ,试验缄比对照组分别提高３０．０３ｋｇ和１６６．８２ｇ差异显著  相似文献   

A review of antimicrobial peptides: defensins and related cationic peptides     

Evans EW  Harmon BG 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1995,24(4):109-116

Cationic antimicrobial peptides are present throughout the plant and animal kingdoms and bear striking structural and functional similarities across species lines. They provide primitive, nonspecific means of combating a variety of bacteria, fungi, enveloped viruses, and protozoa. Some are also cytotoxic against host cells, including neoplastic cells. Cationic antimicrobial peptides may play various roles in inflammation and tissue repair. Antimicrobial peptides are found in epithelial tissues regularly exposed to microbial attack as well as in cells whose primary function is defense against potential pathogens. They constitute an important part of the nonoxidative antimicrobial arsenal of leukocytes. They are preformed and/or readily synthesized when the cells are stimulated by exposure to pathogens. They exert their effects directly by inserting into membranes of target cells and forming ion channels which increase membrane permeability; however, antimicrobial peptides can also act as opsonins to facilitate phagocytosis. Resistance to defensins is a virulence factor for organisms such as Salmonella sp. The study of cationic antimicrobial peptides is increasing our understanding of innate immunity, inflammation, and the pathogenesis of genetic diseases such as specific granule disease in humans. Therapeutic applications of antimicrobial peptides are currently under investigation.  相似文献