胆盐和游离脂肪酸影响离体小肠细胞类胡萝卜素吸收的研究   总被引：6，自引：0，他引：6  

陈波  周光宏  刘清 《动物营养学报》2001,13(2):47-50

用黄鸡小肠细胞进行了 β-胡萝卜素和黄体素的体外吸收试验 ,研究类胡萝卜素浓度、胆盐和游离脂肪酸对这两种类胡萝卜素吸收的影响。结果发现 :β-胡萝卜素和黄体素的吸收均呈浓度依赖性 ;牛磺胆酸钠极显著促进黄鸡离体小肠细胞类胡萝卜素的吸收(P<0.01) ,其促进 β-胡萝卜素和黄体素吸收的最佳浓度分别为3mM和10mM ;游离脂肪酸极显著促进了类胡萝卜素的吸收(P<0.01) ,其中油酸对类胡萝卜素的促吸收作用大于硬脂酸 ,促进 β-胡萝卜素和黄体素吸收的最佳油酸/硬脂酸比率分别为100 :0和3 :1。牛磺胆酸钠和游离脂肪酸影响 β-胡萝卜素吸收的动态变化模式与它们对黄体素的显著不同  相似文献   

Evaluation of β‐carotene assimilation in leopard geckos (Eublepharis macularius)          下载免费PDF全文

Ophélie Cojean  Stéphane Lair  Claire Vergneau‐Grosset 《Journal of animal physiology and animal nutrition》2018,102(5):1411-1418

Although leopard geckos (Eublepharis macularius) are commonly kept under human care, their vitamin requirements are largely unknown. Many invertebrate preys display a low vitamin A concentration; thus, gut‐loading insects with vitamin A or carotenoids is a common practice. The objective of this prospective experimental study was to investigate whether dietary supplementation with β‐carotene, including prey gut‐loading, leads to sufficient vitamin A hepatic storage and prevents epithelial squamous metaplasia development in leopard geckos. Ten clinically healthy female leopard geckos were randomly divided in two groups with various supplementations: a group receiving vitamin A supplementation and a group receiving β‐carotene. Insects were gut‐loaded continuously with a supplement containing vitamin A or β‐carotene, depending on the group. Oral supplementation with cod liver oil or carrot juice was administered weekly to each lizard from “vitamin A group” and “carotenoid group” respectively. After 10 weeks of supplementation, surgical hepatic biopsies were obtained in three geckos of each group while the two remaining geckos were euthanized to undergo complete necropsy. Hepatic vitamin A concentration was determined for each lizard (n = 10) by ultra‐performance liquid chromatography. Histopathology revealed hepatocellular vacuolization and vitellogenic follicles in five females. Epithelial squamous metaplasia was not observed in any of the geckos. Hepatic vitamin A concentration was significantly higher in the carotenoid‐supplemented group than in the vitamin A‐supplemented group (p = 0.03). Our results suggest that in leopard geckos, dietary supplementation with β‐carotene allows sufficient vitamin A hepatic storage.  相似文献   

红叶石楠提取类胡萝卜素的工艺条件研究     

蒋耀瑛  霍湘芸  董子钰  范晓越  黄志伟  王岁楼 《农产品加工．学刊》2020,(1):29-31,36

在单因素试验基础上,采用正交试验优化红叶石楠绿叶中类胡萝卜素的提取工艺条件,并用柱层析分离纯化粗提液中的类胡萝卜素。结果表明,在40℃下以丙酮∶乙醇=1∶1为提取剂,料液比1∶10,浸提时间1.5 h,类胡萝卜素含量可达51.26μg/g。该类胡萝卜素粗提液经过柱层析法初步分离纯化,纯化倍数可达1.41。  相似文献   

Digestibility,growth and pigmentation of astaxanthin,canthaxanthin or lutein diets in Lake Kurumoi rainbowfish,Melanotaenia parva (Allen) cultured species          下载免费PDF全文

Nina Meilisza  Dedi Jusadi  Muhammad Zairin Jr.  I Made Artika  Nur Bambang Priyo Utomo  Tutik Kadarini  Muhammad Agus Suprayudi 《Aquaculture Research》2017,48(11):5517-5525

New cultured ornamental fish namely Lake Kurumoi rainbowfish Melanotaenia parva (Allen) run into reduced of colour performances when reared in the aquaria, consequently, fish feed must be added with carotenoids as a pigment source. The aim of this study was to evaluate the digestibility, growth and pigmentation of astaxanthin, canthaxanthin and lutein in diet. Apparent digestibility coefficients (ADC) of dry matter, lipid, protein, carotenoids, growth and pigmentation were studied in twenty fish after 14 and 56 days of observation. The single‐dose supplementation of 100 mg/kg of astaxanthin, canthaxanthin, or lutein diets on fish was fed by apparent satiation. The basal diet without carotenoids was used as control. The result showed that the ADC of carotenoids of test diets was higher compared to control. Fish fed astaxanthin diet had higher survival rate (96.67 ± 2.89%), colour measurements of lightness (57.60 ± 7.46%), a*‐values (4.66 ± 1.20), total carotenoids content in skin (33.75 ± 5.02 mg/kg) and muscle (2.16 ± 0.74 mg/kg). Astaxanthin also increased the growth after 14 days (2.00% ± 0.19%/days) but there was no significantly different at the end of experiment. The yellowish‐orange colour performance was more rapidly achieved by fish fed astaxanthin diet after 28 days experimentation. These values suggested that dietary carotenoids were required and astaxanthin diet was superior to other diets for skin pigmentation of Lake Kurumoi rainbowfish.  相似文献   

海洋胶红酵母Y2产类胡萝卜素发酵促进剂的研究     

杨铿  陈永青  梁晓华  杨莺莺  佘妙虹 《南方水产》2013,9(2):45-49

研究了几种添加物对海洋胶红酵母（Rhodotorulamucilaginosa）Y2发酵产类胡萝卜素的促进作用。在摇瓶条件下,发酵培养基中分别添加不同量的番茄汁、花生油、大豆油、玉米浆、谷氨酸钠和生物素,培养72h后取样分别测定类胡萝卜素产量及生物量。结果显示,当分别添加2mL·L-1番茄汁、1．5mL·L-1花生油和3mL·L-1玉米浆时,类胡萝卜素产量比对照组提高14．2％、30．3％和21．3％;谷氨酸钠和生物素会抑制海洋胶红酵母Y2类胡萝卜素的合成。因此,番茄汁、花生油和玉米浆是较为经济适用的海洋胶红酵母Y2产类胡萝卜素发酵促进剂,具有广阔的开发应用潜力。  相似文献   

Dietary carotenoids and skin melanin content influence the coloration of farmed red porgy (Pagrus pagrus)     

S. CHATZIFOTIS  I. Vaz JUAN  P. KYRIAZI  P. DIVANACH  M. PAVLIDIS 《Aquaculture Nutrition》2011,17(2):e90-e100

The present study investigates the effects of dietary carotenoid sources on the coloration of the red porgy, Pagrus pagrus. Red porgies (131.9 ± 16.2 g; mean ± SD) were fed for 12 weeks on five different diets supplemented with red carotenoids (mainly astaxanthin esters) supplied from Haematococcus pluvialis algae and yellow carotenoids (mainly β‐carotene, lutein, and zeaxanthin) supplied from Alfalfa, Medicago sativa L. and Spirulina, Spirulina pacifica. The carotenoid‐supplemented diets did not have any marked effect on the growth rate, the feed conversion ratio, the daily feeding rate, or the hepatosomatic index of red porgy. The biochemical indices measured in plasma including cholesterol, total proteins, glucose, lactate, phospholipids, non‐esterified fatty acids, triglycerides, and thyroid hormones did not differ significantly among groups. Diet did not affect significantly the melanophore‐area coverage, the melanin skin concentration and skin lightness. Carotenoid‐supplemented diets affected significantly the carotenoid deposition in the skin, the presence and distribution of erythorphores and xanthophores, and skin hue and chroma. Overall, data have shown the efficacy of Haematococcus algae in promoting a reddish coloration in red porgy.  相似文献   

Enhancing the mineral and vitamin content of wheat and maize through plant breeding   总被引：5，自引：1，他引：5  

J.I. Ortiz-Monasterio  N. Palacios-Rojas  E. Meng  K. Pixley  R. Trethowan  R.J. Pea 《Journal of Cereal Science》2007,46(3):293

More than half of the world's population suffers micronutrient undernourishment. The main sources of vitamins and minerals (iron, zinc, and vitamin A) for low-income rural and urban populations are staple foods of plant origin that often contain low levels or low bioavailability of these micronutrients. Biofortification aims to develop micronutrient-enhanced crop varieties through conventional plant breeding. HarvestPlus, the CGIAR's biofortification initiative, seeks to breed and disseminate crop varieties with enhanced micronutrient content that can improve the nutrition of the “hard to reach” (by fortification or supplementation programmes) rural and urban poor in targeted countries/regions. In attempting to enhance micronutrient levels in maize and wheat through conventional plant breeding, it is important to identify genetic resources with high levels of the targeted micronutrients, to consider the heritability of the targeted traits, to explore the availability of high throughput screening tools and to gain a better understanding of genotype by environment interactions. Biofortified maize and wheat varieties must have the trait combinations which encourage adoption such as high yield potential, disease resistance, and consumer acceptability. When defining breeding strategies and targeting micronutrient levels, researchers need to consider the desired micronutrient increases, food intake and retention and bioavailability as they relate to food processing, anti-nutritional factors and promoters. Finally, ex ante studies are required to quantify the burden of micronutrient deficiency and the potential of biofortification to achieve a significant improvement in human micronutrient status in the deficient target population in order to determine whether a biofortification program is cost-effective.  相似文献   

猕猴桃果肉颜色相关色素代谢研究进展   总被引：1，自引：1，他引：1  

张计育莫正海  宣继萍贾晓东刘永芝  郭忠仁 《中国农学通报》2013,29(13):77-85

猕猴桃含有独特的风味和丰富的营养价值,深受消费者的亲睐。猕猴桃果肉颜色各异,系统叙述了猕猴桃果肉颜色的种类、果肉颜色在发育过程中的变化、显微结构的变化、色素类物质的种类和变化、以及叶绿素、类胡萝卜素、和花色素苷代谢途径及其分子调控机理。提出了猕猴桃果肉颜色研究现阶段存在的问题,并对其研究前景进行了展望。  相似文献   

不同因素对磨盘柿色素稳定性的影响     

闫小艳  李宝  冷平  梁学军  高琪洁  张丽珍 《中国农业大学学报》2004,9(6):49-52,75

通过对磨盘柿果实色素的乙醇提取液进行不同处理 ,对果实色素的稳定性进行了研究。在阳光直射 5d以内、室内自然光照射 2 5d以内或在室内避光保存 30d以内的条件下 ,磨盘柿色素在可见光区的最大吸收波长未发生改变 ,但吸光值降低 ,柿色素保留率在 6 0 %以上 ;80℃以上的高温对柿色素有破坏作用 ,但 6 0℃ ,2h以内的高温条件下柿色素的稳定性良好 ;柿色素溶液在pH 6～ 11范围内呈稳定状态 ;供试的 9种金属离子中 ,铁离子对柿色素的稳定性影响最大 ,0 12 5mmol·L-1以上的铁离子使柿色素溶液变浑浊 ,出现沉淀 ,Cu2 + ,Ca2 + 和K+ 次之 ,而Al3 + ,Na+ ,Zn2 + 和Mg2 + 影响较小。质量分数为 1%的苯甲酸钠和山梨酸钾对柿色素稳定性影响不大 ;而质量分数为 2 %的VC对柿色素有保护作用。在加工利用和保存柿色素时 ,需提供有利柿色素的稳定条件 ,才能保持柿色素的营养价值。  相似文献   

菠萝蜜类胡萝卜素检测方法研究及呈色物质分析     

胡丽松  吴刚  郝朝运  范睿  伍宝朵  谭乐和 《热带作物学报》2017,38(5):950-956

为研究菠萝蜜果肉类胡萝卜素分析方法、分析呈色组分,本研究基于高效液相色谱法检测果肉中类胡萝卜素组分,通过比较不同色泽果肉中类胡萝卜素物质差异,鉴定呈色组分。结果表明,用含0.01%BHT的正己烷∶丙酮∶无水乙醇(2∶1∶1,V/V/V)作为提取液,结合超声震荡、皂化脱脂等步骤,能有效提取菠萝蜜果肉中总类胡萝卜素。组分分析采用YMC carotenoid column C30(4.6×250 mm)色谱柱,二极管阵列检测器(photodiode array,PDA)在450 nm波长下,检测出16种不同的类胡萝卜素组分。定量结果表明,β-柠乌素是红色果肉的呈色组分,紫黄质是黄肉果肉主要的呈色组分。差异组分之间存在一定的代谢关联,即紫黄质合成前体的裂解反应及下游异构化反应是造成果肉色泽差异的潜在原因。  相似文献