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排序方式: 共有362条查询结果,搜索用时 15 毫秒
1.
Tayita Suttirojpattana Theesit Juanpanich Rangsun Parnpai Teraporn Vutyavanich 《Animal Science Journal》2021,92(1):e13585
Two-cell stage and blastocyst stage mouse embryos were equilibrated in a medium containing 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO) for 8–15 min. Vitrification was performed in a medium containing 0.5 M sucrose and either 15% EG + 15% DMSO, 17.5% EG + 17.5% DMSO, or 20% EG + 20% DMSO for 30 s. They were then placed either on a hemi-straw (HS) or a hollow fiber vitrification (HFV) device and vitrified by cooled air inside a 0.5-ml straw. In two-cell embryos, a 100% survival rate was obtained from all groups except the 20% HS group (P > .05). All vitrified two-cell groups showed similar rates of blastocyst development to that of fresh control group (P > .05), except 17.5% and 20% HFV groups, which were significantly lower than the other groups (P < .05). In the blastocyst embryos, the HFV groups were divided into two subgroups (non-collapsed; HFV-NC and collapsed; HFV-C blastocyst). Re-expansion rate in 15% HFV-NC, 17.5% HFV-NC, and 15% HFV-C groups was reduced (P < .05), whereas the rest were similar to control. In conclusion, we established a simplified, reliable, and closed system for HFV vitrification applying hemi-straw, which does not require skilled practitioners. 相似文献
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Masahiro SATOH 《Animal Science Journal》2004,75(2):97-102
The present study evaluated the advantage of mixed‐model techniques over a selection index under different magnitudes of an additional systematic environmental effect (ASEE) in terms of accuracy of prediction and expected genetic gain. The data attempted to simulate a closed herd in a pig breeding program. The base population (G0) consisted of 10 males and 50 females. Six generations (G0 to G5) were selected by using a selection index of three traits without overlapping. Additional systematic environmental constants with four levels in a generation were assigned from a uniform distribution at different ranges. Breeding values of animals in the last generation (G5) were estimated on the basis of an index of individual phenotype (SI‐U), SI‐U adjusted for ASEE using a least‐squares mean (SI‐A), best linear unbiased prediction using an animal model excluding ASEE (AM‐E), and an animal model including ASEE (AM‐I). Accuracy of prediction and expected genetic gain were larger by the animal model than by the selection index, even if heritability of the traits selected was high and ASEE was set to zero. When ASEE was zero, the accuracy of prediction and expected genetic gain given by SI‐U and AM‐I were similar to those given by SI‐A and AM‐E, respectively. However, the differences in accuracy and expected gain between SI‐U and AI‐A and between AM‐I and AM‐E increased as the range of ASEE increased. It was concluded that selection based on an animal model was more effective than index selection, even if the herd environment was uniform and traits with high heritability were selected, and that it should be always included in an evaluation model, however slight any systematic environmental effect may be in a closed herd. 相似文献
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Improving management for higher reproduction accelerates genetic improvement in closed herd of swine
Masahiro SATOH 《Animal Science Journal》2004,75(6):499-502
The present study compared responses to selection at different conception rates and litter sizes at weaning in a simulated closed herd in a swine breeding program. The base population consisted of 10 males and 50 females, and 10 generations of selection was practiced by using individual phenotype or best linear unbiased prediction of breeding values for a trait with heritability (h2) of either 0.2 or 0.5. The probability of conception in a single mating was assumed to be 0.8, 0.9 or 1.0. Litter size at weaning was sampled randomly from a normal distribution with mean 8, 10 or 12 and variance 8.1225. Genetic response increased by approximately 6% for h2 = 0.2 and approximately 5% for h2 = 0.5 at generation 10 when conception rate was increased from 0.8 to 1.0. However, litter size at weaning did not affect response to selection. In conclusion, improving conception rate by environmental management increases genetic response indirectly in a breeding program of a closed swine herd. 相似文献
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为了实现机器人玉米秸秆行的精确定位,对耕作玉米机器人的结构进行了改进,并提出了一种基于泰勒级数展开式的RSSI定位方法,提高了机器人玉米秸秆行的定位精度。定位系统使用高清晰度的摄像机采集图像,并采用PID闭环反馈的方式控制机器人的位移,利用PC主控端图像处理,实现了实时定位功能。为了验证机器人玉米秸秆行定位的可靠性,采用田间试验的方法对机器人的性能进行了测试。结果表明:RSSI定位方法的定位精度较高,且图像处理系统可以准确地标定玉米秸秆行,实现机器人在玉米田中的精确定位,避免了机器人在作业过程中对农作物造成损害。 相似文献
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闭式脱粒滚筒的静动态性能分析 总被引:1,自引:0,他引:1
针对联合收割机中闭式脱粒滚筒有可能在外载荷作用下会发生较大变形和在外激励作用下发生共振的问题,采用SolidWorks软件对闭式脱粒滚筒进行了三维建模,以*.x_t格式导入Ansys Workbench建立有限元模型,然后对闭式脱粒滚筒进行静动态性能分析(即静态分析和模态分析)。静态分析结果表明,闭式脱粒滚筒的刚度和强度足够,变形均在设计允许范围之内;模态分析得到的闭式脱粒滚筒前6阶固有频率和振型结果表明,在规定的工况范围内,闭式脱粒滚筒在外激励作用下不会产生共振。研究结果为后续样机的设计和改进提供了理论依据。 相似文献
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Polymethylmethacrylate orbital implants with interconnecting channels. A retrospective study following enucleation in dogs and cats 下载免费PDF全文
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An orbital sialocele developed in a dog following enucleation for protracted glaucoma. This eye had historically been treated for keratoconjunctivitis sicca by parotid duct transposition approximately 5 years previously, and the duct was ligated distally at the time of enucleation. One month following enucleation, the dog presented with a fluctuant conical-shaped swelling ventrolateral to the orbital socket. Surgical exploration revealed a dilated, fibrotic distal portion of the previously transposed parotid duct, and saliva, within the enucleated orbit. The distal portion of the duct and saliva-containing tissues from within the orbit were excised. The remaining proximal normal portion of the parotid duct was re-routed into the oral cavity. Clinicopathologic and histologic examination of the excised orbital contents and dilated portion of duct revealed a sterile sample of saliva and moderate chronic periductal fibrosis. At a 6-month re-evaluation there was no evidence of recurrence of the sialocele, and the parotid duct was functional. 相似文献
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