Identification of a class of CFEM proteins containing a new conserved motif in Fusarium oxysporum     

《Physiological and Molecular Plant Pathology》2015

Fusarium oxysporum (Fo) is an important soil-borne fungus that cause huge economic losses worldwide. Fungal-specific Common in Fungal Extracellular Membrane (CFEM) proteins are known to be involved in some important physiological processes associated with pathogenicity. To date, few Fo CFEM proteins have been characterized. The recent publication of several genomes of Fo has allowed us to conduct a genome-wide comparison analysis of CFEM proteins in Fo. In this study, we identified CFEM proteins for 12 different Fo formae speciales(f. sp) and obtained an average of 16 CFEM proteins for each Fo. The Fo CFEM proteins were classified into three groups (groups 1–3) according to structural features. Importantly, we identified a new conserved motif containing about 50 amino acids (DR motif) in group 1 members. CFEM proteins containing DR motif (CFEM_DR proteins) are remarkably conserved among Fo, and their number is greater in Fo compared with other fungi. We found the expansion of CFEM_DR proteins in Fo can be attributed to the segmental duplications in the genomes. Expression analysis revealed that CFEM_DR genes had a higher expression level in mycelium than conidia, and their expressions increased dramatically in the host roots at 3 days post inoculation, indicating that Fo CFEM_DR genes have roles while colonizing and infecting their hosts.  相似文献   

Non‐communicating gastric duplication cyst in a 10‐week‐old Labrador Retriever puppy          下载免费PDF全文

MW Jack  D Burgess  A Griffin 《Australian veterinary journal》2016,94(5):166-170

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Genome-wide analysis of the putative AP2/ERF family genes in Vitis vinifera     

Jing Zhuang  Ri-He Peng  Zong-Ming Cheng  Jian Zhang  Bin Cai  Zhen Zhang  Feng Gao  Bo Zhu  Xiao-Yan Fu  Xiao-Feng Jin  Jian-Min Chen  Yu-Shan Qiao  Ai-Sheng Xiong  Quan-Hong Yao 《Scientia Horticulturae》2009,123(1):73-81

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5种蔷薇科树种多酚氧化酶比较基因组学分析     

闫洪波  李桂琴  宋亚伟  孟钰玉  王云松  关军锋 《热带作物学报》2017,(2):320-327

蔷薇科植物中有多种具有重要价值的水果,如苹果、梨、桃、草莓和黑树莓等。这些水果普遍容易发生由多酚氧化酶(PPO)介导的酶促褐变而导致重大经济损失,从全基因组角度分析比较PPO基因家族,有助于加深对PPO基因家族和功能的认识。采用比较基因组学的方法,对这5种植物的PPO基因家族进行了基因鉴定、染色体定位、编码蛋白的亚细胞定位、内含子统计分析、基因系统进化、基因倍增与丢失等特征分析。从这5种植物中,共计鉴定出了42个PPO基因,其中6个基因被认定是假基因;绝大多数基因编码的蛋白定位于叶绿体,2个定位于线粒体,3个是分泌型蛋白;PPO基因在染色体上有串联重复和散布两种形式;9个基因具有内含子,聚类结果显示可以将它们分为6个类型,每个基因类型在进化过程中都发生过基因倍增和丢失;同型基因内含子的位置和大小具有相似性。这些结果揭示蔷薇科植物PPO基因内含子是伴随着基因倍增产生的,基因倍增也是推动PPO基因多样化的重要动力,PPO基因倍增和丢失差异导致PPO基因数量在不同物种之间产生差异。  相似文献   

大豆Abhydrolase_3基因家族进化分析     

王娇  张培培  程浩  喻德跃 《大豆科学》2017,36(6)

异黄酮是大豆的重要次生代谢物,参与植物与微生物互作。2-羟基异黄酮脱水酶(hydroxyisoflavanone dehydratase,HID)催化2-羟基异黄酮形成稳定的异黄酮。HID属于Abhydrolase_3基因家族,该基因家族具有多种功能,但该基因家族在大豆中的进化模式尚待研究。为了研究Abhydrolase_3基因家族在大豆中的进化模式,本文在大豆基因组中鉴定了62个Abhydrolase_3基因,串联和片段复制是该基因家族主要扩增方式。根据系统进化关系,将大豆Abhydrolase_3基因家族划分为8个亚家族,其中HID所在的亚家族I基因数量最多,并发生多次基因扩增事件。对大豆Abhydrolase_3基因家族结构分析表明,不同亚家族具有不同的基序。多态性分析表明,亚家族Ⅰ、Ⅲ和Ⅴ具有较高的核苷酸差异,并受到放松的自然选择。基因表达分析表明,除了亚家族II和IV外,其它亚家族的基因在大豆不同组织中有较高表达;亚家族Ⅰ、Ⅲ、Ⅳ、Ⅴ和Ⅵ基因受病原菌诱导表达。结果说明HID所在的亚家族I存在基因扩增和功能分化,与病原菌互作相关的基因具有较高的遗传多样性并受病原菌诱导表达。  相似文献   

4个物种CCT结构域基因家族的序列进化分析     

陈华夏  申国境  王磊  邢永忠 《华中农业大学学报》2010,29(6):669-676

通过分析4个完成测序和注释的植物基因组,系统地分离鉴定了97个水稻、玉米、高粱和拟南芥的CCT结构域基因,并对相应蛋白质的结构和基因之间的系统演化关系进行了分析。结果表明:CCT结构域基因的蛋白质结构和特性在不同物种之间具有广泛的变异;不同基因组中CCT结构域基因通过染色体复制扩展了基因家族成员。根据其CCT结构域分为4组,其中1个亚组集中了绝大部分的禾本科CCT结构域基因,该亚组基因可能特异参与了禾本科作物开花时间的调控。基因家族成员的扩展,蛋白质结构的改变以及基因表达模式的变异共同导致了CCT结构域基因家族成员在物种间和物种内的功能分化。  相似文献   

Ancient Polyploidy and Modern Crop Plants     

The growing set of fully-sequenced angiosperm genomes highlight the role of polyploidy in angiosperm evolution    suggest that even the high level of importance we had already attributed to this mechanism was inadequate.…… 《分子植物育种》2007,5(2):151

The growing set of fully-sequenced angiosperm genomes highlight the role of polyploidy in angiosperm evolution, and suggest that even the high level of importance we had already attributed to this mechanism was inadequate.  相似文献   

Duplication of the Bx7 high-molecular-weight glutenin subunit gene in bread wheat (Triticum aestivum L.) cultivar'Red River 68'   总被引：5，自引：0，他引：5  

R. D'Ovidio    S. Masci    E. Porceddu  D. D. Kasarda 《Plant Breeding》1997,116(6):525-531

SDS-PAGE analysis of seed proteins of the cultivar‘Red River 68’showed a considerably higher staining intensity of the band corresponding to HMW-GS Bx7 relative to the equivalent band in the cultivars‘Chinese Spring’and‘Cheyenne’. Southern blots of restriction enzyme fragments from DNA of these three cultivars were analyzed densitometrically to reveal that the band corresponding to the Bx7 gene of‘Red River 68’had a double staining intensity compared to the equivalent bands from the other two cultivars, which indicates that in‘Red River 68’a duplication of the Bx7 gene has occurred. Although the possibility of the gene copy being a pseudogene was not ruled out, the greater amount of protein corresponding to Bx7 in‘Red River 68’most likely is in accord with an increase in active gene number. SDSPAGE analysis of the proteins showed also that the mobility of Bx7 in‘Cheyenne’was slightly different from the mobilities of the Bx7 subunits of‘Red River 68’and‘Chinese Spring’. The same difference was observed at the gene level by PCR amplification of the genes encoding these subunits.  相似文献   

Ancient Polyploidy and Modem Crop Plants     

A.H. Paterson 《分子植物育种》2007,5(2):151-151

The growing set of fully-sequenced angiosperm genomes highlight the role of polyploidy in angiosperm evolution, and suggest that even the high level of importance we had already attributed to this mechanism was inadequate. Gene loss following whole-genome duplication events has contributed much to the incongruities that we observe in comparative genetic maps, and presumably has also contributed to functional divergence of taxa. Ancient genome duplication has several consequences for levels and patterns of variation in modern plant populations, in particular contributing to the relatively conservative evolution of duplicated genes even at distant locations from one another in the genome. Comparison of gene retention/loss following independent whole-genome duplication events suggests patterns that may be relevant to the survival and productivity of newly-formed polyploids, with numerous implications for crop improvement.  相似文献   

玉米脱水素基因家族的鉴定与分析   总被引：2，自引：0，他引：2  

赵阳  王玉  蔡慧林  程备久  马庆 《安徽农业大学学报》2015,42(5):657-665

脱水素(dehydrin,DHN)属于LEA蛋白第二家族成员,是一种植物中广泛存在的亲水性蛋白,在干旱、低温和高盐等非生物胁迫的环境中起到重要作用。通过生物信息学方法对玉米全基因组DHN家族成员进行了鉴定,并进一步对其系统发育、基因结构、染色体定位和基因复制以及表达模式进行了系统分析。结果显示,在玉米DHN基因家族中共有5个家族成员,多物种系统发育进化树分析、基因结构以及基序分析都表明DHN家族成员在进化上具有高度的保守性。基因复制和物种间微共线性分析表明,在5个玉米DHN基因中存在着1对片段复制基因(ZmDHN1-ZmDHN2),玉米、高粱和水稻3个物种间存在2对直系同源基因(ZmDHN2-Sb04g032250.1,ZmDHN2-Os02g44870.1)。通过转录组表达数据分析表明,玉米DHN家族基因在不同发育时期具有不同的组织表达模式;同时,诱导表达模式分析表明ZmDHN基因的表达受到盐和干旱胁迫的显著诱导。该研究结果将为进一步鉴定玉米DHN家族重要的基因成员并对其开展功能分析奠定基础。  相似文献