摆动平底从动件盘形凸轮廓解析设计的应用     

殷珍荣  张凤举  刘金国 《扬州大学学报(农业与生命科学版)》1989,(3)

本文根据具体机器动作协调性要求及其具体空间几何条件,采用了摆动从动件盘形凸轮机构。其摆杆推程在O°～52°及52°～62°范围内分别采用了等加速——等速合理拼接运动规律。回程在62°～153°范围内采用了正弦加速度运动规律。用极坐标矢量分析法建立方程、微分,通过电脑程序计算,设计制造出凸轮轮廓,用于禾苗移植机的擒纵机构处,完全符合生产要求,获得了理想的效果。  相似文献   

切割取样对胚胎发育的影响   总被引：2，自引：0，他引：2  

程金华  王栋  朱化彬  郝海生 《中国畜牧杂志》2006,42(19):18-20

体视显微镜下,采用徒手持金属刀片和自制的玻璃切割针分别对小鼠和牛胚胎进行切割取样,并对取样后的胚胎进行体外培养48h,其发育率分别为76.7%(46/60)和80%(16/20),两者差异不显著(P>0.05);奶牛新鲜胚胎切割取样后,胚胎移植妊娠率47.1%(8/17),比对照组妊娠率59.0%(23/39)差异显著(P<0.05)。冷冻-解冻胚胎切割取样后移植妊娠率42.9%(6/17)虽然也比对照组50%低,但是差异不显著(P>0.05)。  相似文献   

PGF2αeCG及hCG 3种生殖激素联用对母犬发情、卵泡发育及胚胎着床的影响   总被引：3，自引：0，他引：3  

谭建华  安铁洙  刘珠果  万忠海  周虚 《中国兽医学报》2002,22(4):394-396

给休情期性成熟母犬肌注 PGF2α(0 .5 m g/ kg) ,2 4 h后肌注 e CG(5 0 IU/ kg) ,再经 12 0 h后肌注 h CG(2 0 U/ kg) ,诱导其发情。将诱导发情的母犬与公犬交配 ,于交配后 16 d,摘取卵巢、子宫 ,观察其卵泡发育和胚胎着床情况。结果表明 ,药物处理可明显促使母犬提前结束休情期而进入发情期 (9/ 10 ) ,促进卵巢内卵泡发育 (P<0 .0 1) ,并使更多的胚胎着床 ,每只母犬子宫胚胎着床点为 (17.4± 2 .2 )个 ,提示 3种生殖激素联合应用可提高母犬繁殖率  相似文献   

Weeding techniques in transplanted and direct wet-seeded rice in Pakistan     

MOHAMMAD SAFDAR BALOCH  GUL HASSAN  TETSUO MORIMOTO 《Weed Biology and Management》2005,5(4):190-196

Field studies were undertaken to explore the weed management strategies for transplanted and direct wet-seeded rice in the Dera Ismail Khan district of North-west Frontier Province, Pakistan. The experimental design was a randomized complete block with a split plot arrangement. The main plots consisted of two planting techniques (transplantation and direct seeding), while weed control practises assigned to the subplots included the use of the granular herbicide Sunstar 15WG (ethoxy sulfuron), Machete 60EC (butachlor), conventional hand weeding, and the weedy check (untreated control). Data were recorded on weed dynamics and the agronomic parameters of the rice crop. Economic analyses on the data were also run. The weed density and biomass were lower in the transplantation plots than the direct-seeding plots. The herbicides ethoxy sulfuron and butachlor reduced the density over the weedy check, with a density comparable to hand weeding in 2002, but slightly higher in 2003. As a result of weed management by hand weeding and herbicides, the paddy yield and its components were significantly higher for the transplanted method compared to the direct-seeded method. The comparable yield of herbicides with hand weeding offers an option for the use of herbicides as an alternative management tool. In light of our findings, it is concluded that for good economic returns, rice crops may be transplanted rather than direct-seeded. The herbicides ethoxy sulfuron and butachlor offer a weed control cover comparable with hand weeding under the transplantation method, while butachlor is good for controlling weeds even under the direct-seeded conditions in the agroclimatic conditions of the area.  相似文献   

Based serum metabolomics analysis reveals simultaneous interconnecting changes during chicken embryonic development          下载免费PDF全文

M. L. Peng  S. N. Li  Q. Q. He  J. L. Zhao  L. L. Li  H. T. Ma 《Journal of animal physiology and animal nutrition》2018,102(5):1210-1219

Metabolic disorder is a major health problem and is associated with a number of metabolic diseases. Due to native hyperglycaemia and resistance to exogenous insulin, chickens as a model had used in the studies of adipose tissue biology, metabolism and obesity. But no detailed information is available about the comprehensive changes of serum metabolites at different stages of chicken embryonic development. This study employed LC/MS‐QTOF to determine the changes of major functional metabolites at incubation day 14 (E14d), 19 (E19d) and hatching day 1 (H1d), and the associated pathways of differential metabolites during chicken embryonic development were analysed using Metabolite Set Enrichment Analysis method. Results showed that 39 metabolites were significantly changed from E14d to E19d and 68 metabolites were significantly altered from E19d to H1d in chicken embryos. Protein synthesis was promoted by increasing the concentrations of L‐glutamine and threonine, and gonadal development was promoted through increasing oestrone content from E14d to E19d in chicken embryos, which indicated that serum glutamine, threonine and oestrone contents may be considered as the candidate indicators for assessment of early embryonic development. 2‐oxoglutaric acid mainly contributed to enhancing the citric cycle, and it plays an important role in improving the growth of chicken embryos at the late development; the decreasing of L‐glutamine, L‐isoleucine and L‐leucine contents from E19d to H1d in chicken embryonic development implied their possible functions as the feed additive during early posthatch period of broiler chickens to satisfy the growth. These results provided insights into understand the roles of serum metabolites at different developmental stages of chicken embryos, it also provides available information for chicken as a model to study metabolic disease or human obesity.  相似文献   

瘤胃液移植在反刍动物中的研究进展     

李岩  杨英魁  柴沙驼  王书祥 《动物营养学报》2023,(4):2105-2110

瘤胃液移植是一种将健康供体动物的瘤胃液转移给受体动物的技术。目前，针对瘤胃液移植的报道还相对有限，但越来越多的研究结果显示，瘤胃液移植可以影响反刍动物的干物质采食量、产奶量等，还具有重建瘤胃菌群结构的潜力。本文综述了瘤胃液移植的方法及实践中的应用研究，分析其对反刍动物生长性能、瘤胃发酵、瘤胃微生物区系的影响以及在治疗某些疾病方面的作用，为反刍动物瘤胃液移植的研究提供参考。  相似文献   

Effects of Donor Cells Treated with Egg Extracts on the Development Competence of Cloned Embryos     

GUO Qin-qin  FAN Zong-xing  Hao Hai-sheng  LIU Yan  ZHAO Xue-ming  ZHU Hua-bin  DU Wei-hua 《中国畜牧兽医》2016,43(2):477-486

Japanese Balck cattle fetal fibroblasts (JBCFF) were induced with Xenopus leavis egg extracts and somatic cell nuclear transfer (SCNT) was carried out with the reprogrammed JBCFF as donor cells in order to investigate their effects on SCNT efficiency.Three samples of egg extracts were acquired from different Xenopus laevis.The protein contents and kinds in extracts were evaluated with BCA Protein Quantification Kit and SDS-PAGE.Concentration of Digitonin to permeabilize JBCFF was optimized and assessed with PI staining.Reprogrammed cells treated with egg extract were used as donor in SCNT.Additionally the reconstructed embryos were activated with ionomycin+6-DMAP and A23187+6-DMAP to compare their effects on the development competence.The protein contents of extracts samples were 56.2255,64.6570 and 71.2158 μg/mL,respectively,the each extract had the same composition about 40-55 and 70-100 ku.The optimal concentration of Digitonin was 7 μg/mL and the permeabilization rate was 55.44%.After extracts treatment and continuous culture for 6-7 d,JBCFF formed well-defined colony structures.No significant composition difference was found in rates of fusion (92.83% vs 96.04%),cleavage (89.64% vs 89.78%) and blastocyst formation (24.06% vs 23.12%) of cloned embryos when the colony cells and JBCFF without extracts treatment were used as donor cells (P>0.05).Similarly,the two activation methods had no significant effect on the developmental competence of cloned embryos (cleavage rate 92.16% vs 92.28%,blastocyst rate 23.21% vs 24.18%).Conclusively,Xenopus leavis,egg extracts could induce JBCFF reprogramming to a low differentiated state.However donor cells with reprogramming partially could not improve the development of cloned embryos and its mechanism requires further research.  相似文献   

白檀未成熟胚的器官发生和植株再生     

杨艳  蒋丽娟  汤玉喜  李昌珠  唐洁  李永进 《北方园艺》2016,(5):119-123

以白檀未成熟胚为试材,以改良MS为培养基,研究了白檀未成熟胚的器官发生和植株再生.结果表明:改良MS(mMS)能够很好的诱导愈伤组织,在添加了0.2 mg/L 6-BA+0.1 mg/L NAA的培养基中,诱导率高达92.5％;胚性愈伤组织分化最佳培养基为mMS+0.25 mg/L 6-BA+0.15 mg/L NAA,分化率为72.4％;分化出的胚性愈伤组织在空白mMS培养基上继代培养15 d,76.6％的组织分化出芽,再转入1/2mMS+ 1.5％蔗糖培养基上培养20 d,芽长至1.5 cm.  相似文献   

无患子当年生砧苗嫁接育苗技术   总被引：4，自引：0，他引：4  

林文荣 《亚热带农业研究》2007,3(3):180-183

采用正劈接、短枝腹接、斜劈接、舌接等不同嫁接方法对无患子实生苗即移即接、移后10 d嫁接、地苗嫁接育苗试验,探索提高无患子嫁接成活率的途径。结果表明,用正劈接方法对无患子实生苗即移即接其嫁接成活率高,此嫁接技术可以在无患子嫁接育苗生产中应用。  相似文献   

水牛胚胎体外生产研究综述     

孙燕潘红梅  张家骅 《中国农学通报》2004,20(4):17-17

近年来采用其它家畜上的程序来进行水牛体外胚胎生产（in vitro production,IVP）引起了越来越多的关注。水牛胚胎体外生产已获得了较高的卵母细胞成熟率、受精率和卵裂率,但囊胚发育率和体外胚的移植成功率仍较低,水牛IVP胚胎体外生产效率比黄牛低得多。在IVP技术应用于水牛生产育种之前,还有一些问题必须解决。本文对水牛胚胎体外生产的不同技术,包括未成熟卵母细胞的体外培养成熟和受精以及胚胎体外培养卵裂发育至囊胚等进行综述。文章还讨论了胚胎体外生产存在的问题及可能的解决方案。  相似文献