十里香茶离体培养及再生体系研究     

杨霏  邹瑞  张玥  杨自云  吴田 《种子》2020,(4):46-49,54

为了保护珍贵的十里香茶树资源,掌握利于十里香茶的最优组培条件,本研究对十里香茶组培过程中外植体类型、消毒时间、培养基添加物及培养条件进行了筛选。结果表明,采用轻微木质化的带腋芽茎段经升汞消毒15 min,接种后暗培养5 d,MS+0.2 mg·L^-1 NAA培养基中添加3 mg·L^-1 PVP+2000 mg·L^-1 Vc或者6 mg·L^-1 PVP+3000 mg·L^-1 AC,可在低污染率的前提下将褐化率降为10%左右,一定程度上解决了茶树组培中褐化率高的难题;采用1/8 MS+1 mg·L^-1 IBA培养基有利于外植体生根且增殖迅速。本研究建立了十里香茶的无菌苗离体培养及再生体系,可通过组培手段对其进行大量繁殖。  相似文献   

异硫氰酸丙烯酯毒杀松材线虫作用机制初步研究     

韩双双  谭周倩  黄晓德  钱骅  陈斌  赵伯涛  陈双林  闫淑珍 《农药学学报》2020,22(1):48-53

对异硫氰酸丙烯酯毒杀松材线虫作用机制进行了初步研究。测定了其对线虫虫体形态、运动行为、体内总糖和蛋白质含量的影响,以及处理前后线虫体内过氧化氢酶(CAT)、乙酰胆碱酯酶(AChE)和谷胱甘肽-S-转移酶(GST)活性变化。结果表明:经异硫氰酸丙烯酯处理后的松材线虫虫体形态严重扭曲,运动形态出现由正常的S形变化为多节弯曲和螺旋状卷曲等异常状况,线虫行动随时间的推移变得迟缓,最终呈C形、新月形或直接死亡。随着异硫氰酸丙烯酯处理质量浓度的增大,线虫体内总糖和蛋白质含量显著上升,糖和蛋白质的代谢发生紊乱;线虫体内CAT、AChE和GST活性显著降低,且均低于对照组,表明线虫体内氧化和抗氧化作用失衡;细胞受到极大的影响,神经系统遭到破坏,解毒能力明显降低。研究结果可为研究利用异硫氰酸丙烯酯作为植物源杀线虫剂提供依据。  相似文献   

Effects of muscle types and cooling on discoloration of canned skipjack     

Yeun Suk Gu  Hyoung Sik Yoon  Douck Choun Park  Cheong Il Ji  Tae Yong Cho  Myung Chan Kim  Sung Jae You  Saeng Gyu Yeo  Seon Bong Kim 《Fisheries Science》2001,67(6):1145-1150

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C_R704玉米与S_C704玉米过氧化物酶同工酶及生理指标分析     

刘双俊  兰庭瑶  柯贤波  马立群  王主选 《新疆农业大学学报》1990,(1)

C_R704玉米与S_C704玉米各时期的过氧化物酶同功酶谱无明显差别,二者遗传基础一致,但酶活性C_R704玉米高于S_C704玉米。经测定三系杂种C_R704玉米在叶绿素、糖、淀粉及蛋白质含量均优于单交种S_C704玉米。  相似文献   

Ouabain-sensitive Na/K-ATPase activity increases during embryogenesis in the giant freshwater prawn Macrobrachium rosenbergii     

Marcy N Wilder  Do Thi Thanh Huong  Atsuro Okuno  Muharijadi Atmomarsono  Wei-Jun Yang 《Fisheries Science》2001,67(1):182-184

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Serum bile acids: reference values in healthy dogs and comparison of two kit methods     

Counsell LJ  Lumsden JH 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1988,17(3):71-74

Serum bile acid (SBA) reference intervals were established by use of a radioimmunoassay method for fasting dogs to be 0.2 to 4.3 micro mol/L (n = 60) and for 2 hour postprandial samples to be 0.6 to 24.2 micro mol/L (n = 37). The SBA reference intervals estimated using an enzymatic method were 0 to 8.6 micro mol/L for fasting (n = 26) and 0 to 29.8 micro mol/L for 2 hour postprandial samples (n = 36). The correlation between the two methods including samples from healthy dogs and clinical cases is good (n = 128, r = 0.82, p < 0.0001). The radioimmunoassay method is linear to 50 micro mol/L and the enzymatic method is linear to 100 micro mol/L, thus both methods require serum dilutions to be made in many cases of primary liver disease. The enzymatic method is less expensive and more convenient for use in a clinical laboratory but requires a greater sample volume (400 micro I) than the RIA method (50 micro I). Both methods have adequate precision and accuracy to be useful as diagnostic tests of liver function in dogs.  相似文献   

Colonization and histopathology of susceptible and resistant carnation cultivars infected with Fusarium oxysporum f. sp. dianthi     

R. P. Baayen  D. M. Elgersma 《European journal of plant pathology / European Foundation for Plant Pathology》1985,91(3):119-135

Stems of the susceptible Early Sam and resistant Novada carnations were inoculated with a conidial suspension ofFusarium oxysporum f. sp.dianthi. Stem segments of either cultivar were sampled regularly and used for determination of fungal growth and for microscopical investigation.Early Sam showed typicalFusarium wilt symptoms and its stems were colonized intensively. The observed vascular browning appeared to be caused by discolouration of primary walls of infected vessels and surrounding cells. Vessels were rarely occluded with gel. Cell wall degradation led to the formation of stem cavities. Hyperplasia of xylem parenchyma was not seen.In Novada, fungal colonization remained low throughout the experiment. Macroscopic symptoms were absent except for longitudinal bursts in the stem, which appeared to be caused by hyperplasia of xylem parenchyma bordering infection. Vascular gelation occurred in the infected tissues, causing some vascular browning also. Xylem vessel regeneration was observed in the hyperplastic layer. Cavities were not formed, and wall discolouration was rare. Vascular gelation is considered part of theFusarium wilt resistance mechanism. It is followed by xylem vessel regeneration, which expresses a general plant response to vascular dysfunction rather than being part of the resistance mechanism.Although of different origin, vascular browning as such occurs in both susceptible and resistant interactions. In breeding for resistance, care should hence be taken with the current use of browning as an indication of disease.Samenvatting Anjers van de vatbare cultivar Early Sam en de resistente cultivar Novada werden geïnoculeerd met een conidiënsuspensie vanFusarium oxysporum f. sp.dianthi. Van beide cultivars werden regelmatig stengeldelen geoogst om deze microscopisch te onderzoeken en om de schimmelgroei te bepalen.Early Sam vertoonde de voor deze verwelkingsziekte kenmerkende symptomen en werd intensief gekoloniseerd. Aan het vaatweefsel waargenomen bruinkleuring bleek veroorzaakt te worden door verkleuring van de primaire wanden van geïnfecteerde vaten en de hen omringende cellen. Zelden trad er in de vaten gomvorming op. Celwandafbraak veroorzaakte de vorming van holten in de stengel. Hyperplasie van het houtparenchym werd niet waargenomen.In Novada bleef de schimmelgroei gedurende het hele experiment beperkt. Macroscopisch waren er enkel lengtescheuren in de stengel te zien, die veroorzaakt bleken te worden door hyperplasie van aan de infectie grenzend houtparenchym. In het geïnfecteerde vaatweefsel optredende gomvorming veroorzaakte ook enige bruinkleuring. In het hyperplastische weefsel werd regeneratie van houtvaten waargenomen. In de stengel werden geen holten gevormd, en verkleuring van de celwanden kwam weinig voor. De vorming van gommen in de houtvaten maakt waarschijnlijk deel uit van het resistentiemechanisme. De daarop volgende houtvatregeneratie is eerder een algemene reactie van de plant op vaatverstopping dan een deel van het resistentiemechanisme.Vaatverbruining, zij het van verschillende oorsprong, komt voor in zowel vatbare als resistente interacties. Om die reden moet men in de resistentieveredeling bij de anjer voorzichtig zijn met het gebruik van bruinkleuring als ziekteïndicatie.  相似文献   

以豆粕为原料制备反刍动物饲用肽的研究     

王雅晶  李胜利  骆雅萍  李静 《中国饲料》2005,(20):15-17

本文通过正交试验,选用碱性微生物蛋白酶,研究得出酶解法制备大豆肽的最佳工艺参数:豆粕预处理条件为90℃水浴加热10min,酶解条件为底物浓度5%(W/V)、加酶量5万单位/g蛋白质、温度50℃、pH值10、酶解时间5.5h。蛋白质水解率达到25%,平均肽链长度为4.0。制得大豆肽粗蛋白质含量66.83%(DM)。并对制得大豆肽和原料豆粕的氨基酸含量进行分析。  相似文献   

长期培肥对土壤酶活性的影响   总被引：24，自引：0，他引：24  

邱莉萍  刘军  和文祥  王益权  孙慧敏 《干旱地区农业研究》2003,21(4):44-47

25a的有机肥长期定位试验地中土壤养分和有关酶活性研究表明:耕种和不同的培肥方式均能提高土壤养分的含量,明显地改善土壤的生物活性,其中对土壤脲酶和磷酸酶活性影响明显,多酚氧化酶显著性则较差。对土壤酶活性进行相关分析发现,土壤脲酶和碱性磷酸酶之间呈极显著正相关,且这两种酶与有关主要土壤肥力因子均呈显著或极显著正相关关系。揭示出了土壤脲酶和碱性磷酸酶活性可以用来作为评价土壤肥力的综合指标。  相似文献   

豆粕酶解发酵物对仔猪生长性能、血清指标及肠道菌群结构的影响     

赵娜  杨雪海  陈芳  郭万正  黄少文  张巍  李绍章  严念东  魏金涛 《动物营养学报》2016,(12):242-249

本试验旨在研究豆粕酶解发酵物对仔猪生长性能、血清指标及肠道菌群结构的影响。选用144头49日龄健康的"杜×长×大"三元杂交育肥仔猪,随机分为3组,每组6个重复,每个重复8头猪(公母各占1/2)。阴性对照组饲喂无抗生素添加的基础饲粮,抗生素对照组饲喂基础饲粮+60 g/t吉他霉素,试验组饲喂试验饲粮(基础饲粮中10%豆粕被豆粕酶解发酵物替代)。试验期为42 d。结果表明:1)试验组仔猪的末重、平均日增重、平均日采食量分别比阴性对照组高5.06%、10.07%、5.03%(P0.05),腹泻率比阴性对照组低39.59%(P0.05),与抗生素对照组相比各指标差异均不显著(P0.05)。2)试验组仔猪血清碱性磷酸酶和谷丙转氨酶活性、总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性均显著高于阴性对照组(P0.05),丙二醛(MDA)含量显著低于阴性对照组(P0.05);试验组和抗生素对照组仔猪血清指标差异不显著(P0.05)。3)试验组仔猪盲肠、大肠中乳酸杆菌数量显著高于阴性对照组和抗生素对照组(P0.05),十二指肠中总细菌数显著低于阴性对照组和抗生素对照组(P0.05)。综上,豆粕酶解发酵物能提高仔猪生长性能,增强机体抗氧化能力,利于乳酸杆菌在肠道内形成优势菌群,降低腹泻率,生长性能达到使用抗生素水平。  相似文献