饥饿、季节及性别对大鲵肌肉、肝脏生化成分的影响     

杨代勤 《湖北农学院学报》1990,(3)

季节会影响到大鲵肌肉的脂肪及钙的含量,肝脏的脂肪、钙及游离氨基酸的含量,饥饿对肌肉的酸化成分不产生影响,但饥饿程度的增加,会对肝脏脂肪无氮浸出物及游离氨基酸产生影响;繁殖前雌性大鲵的肌肉肝脏的肪肪含量均高于雄性的。  相似文献   

Annual sex hormonal profiles, gonad development and age determination of the Mekong giant catfish (Pangasianodon gigas, Chevey)     

A Manosroi  K Meng-umphan  & J Manosroi 《Aquaculture Research》2003,34(15):1379-1385

Annual sex hormonal profiles, gonad development and age determination of 18 (13–14 kg body weight) and three (145–226 kg body weight) Mekong giant catfish (MGC) (Pangasianodon gigas, Chevey) reared in earthen ponds in Chiang Mai and from the Mekong River in Chiang Rai, Thailand, respectively, were investigated. The hormonal profiles were determined from blood samples of the fish by electrochemiluminescence immunoassay during May to August in 2000. The highest testosterone levels of 0.06 ng mL^?1 in both sexes and the highest oestradiol of 47.8 and 14.23 pg mL^?1 in females and males, respectively, were observed in May. The gonadosomatic index was found to be 0.07 for males and 0.38 for females from ponds in comparison with 2.27 for males and 8.29 for females from the Mekong River. Higher development stages of spermatocytes and oocytes of the cultured fish in May than in February and November were demonstrated. No mature germ cells were obtained from either the males or females, indicating no sexual maturity. The average age of fish was determined from the annual rings of the cross‐section of the pectoral fins by a stereomicroscope. The average age of fish in earthen pond determined from the annual ring was 8 years, which agreed with the actual culture records, while those from the Mekong River were 21 years. This information will be beneficial for the breeding programmes and conservation of the MGC.  相似文献   

三种融合标签对大熊猫轮状病毒结构蛋白VP6-VP7可溶性表达的影响     

文继锋  申欢欢  龚永平  易可可  杨智捷  邓英  颜其贵 《浙江农业学报》2019,31(7):1073

大熊猫轮状病毒(giant panda rotavirus,GPRV)是引起幼龄大熊猫腹泻的主要病原,对圈养大熊猫产生了较大的危害。轮状病毒结构蛋白VP6是一种载体蛋白,可介导黏膜免疫反应。VP7是轮状病毒结构蛋白中主要的中和抗原。因此,VP6-VP7的融合表达作为候选抗原对该病的防治具有重要的意义。传统大肠埃希菌原核表达存在表达量低、可溶性差以及纯度低等弊端。本研究使用醛缩酶(EDA)、谷胱甘肽S-转移酶(GST)、麦芽糖结合蛋白(MBP)3种融合标签,以实现获得表达量高和纯度高的GPRV-VP6-VP7重组表达蛋白。将扩增的VP6、VP7基因片段利用同源重组酶构建到含3种融合标签的表达载体pET21b上,将重组质粒转化至大肠埃希菌Rosetta(DE3)感受态细胞中进行低温诱导表达。用Ni-柱亲和层析法纯化目的蛋白,SDS-PAGE和Image J分析蛋白表达量和可溶性,Western blot分析得到表达的重组表达蛋白正确且具有蛋白活性。实验结果证明,EDA标签能显著促进VP6-VP7蛋白的原核可溶性表达,提高VP6-VP7蛋白表达量。  相似文献   

Immobilization of wild giant panda (Ailuropoda melanoleuca) with dexmedetomidine–tiletamine–zolazepam          下载免费PDF全文

Yipeng Jin  Yanchao Qiao  Xiaobin Liu  Tianchun Pu  Hongqian Xu  Degui Lin 《Veterinary anaesthesia and analgesia》2016,43(3):333-337

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Effects of brodifacoum and diphacinone exposure on four species of reptiles: tissue residue levels and survivorship     

Richard E Mauldin  Gary W Witmer  Susan A Shriner  Rachael S Moulton  Katherine E Horak 《Pest management science》2020,76(5):1958-1966

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大熊猫铜绿假单胞菌呼吸道感染继发白色念珠菌性肠炎的诊治     

修云芳  邵良平  陈玉村  王德春  徐素慧 《经济动物学报》2006,10(2):103-105

报道了1只大熊猫呼吸道感染,继发肠炎的病例。介绍了其病史,血液学变化和临床症状。经过微生物培养和鉴定,确诊呼吸道感染的致病菌为铜绿假单胞菌,继发性肠炎的致病菌为白色念珠菌。根据药物敏感试验结果,选用阿奇霉素和环丙沙星治愈呼吸道感染;选用氟康唑和金双歧,治愈继发性肠炎。并讨论了铜绿假单胞菌和白色念珠菌的发病原因,总结了临床用药的经验。  相似文献   

Stability comparison of three candidate internal reference genes in Chinese giant salamander (Andrias davidianus)     

Jing Qian  Dun Deng 《Aquaculture Research》2020,51(1):362-369

The Chinese giant salamander (Andrias davidianus) as food and medicinal product has been an important aquaculture object in China. Study of gene function in the Chinese giant salamander requires accurate normalization though the use of appropriate reference genes. In this study, the expression levels of three candidate reference genes including β‐actin, GAPDH and cytb of different tissues, different developmental stages and different challenges in Chinese giant salamander were evaluated by qPCR. The stabilities of these three reference genes were analysed by geNorm, NormFinder and BestKeeper software. The results showed that the expression of GAPDH was more stable than that of β‐actin and cytb in four tissues and at two developmental stages of Chinese giant salamander. Compared with GAPDH and cytb, β‐actin was the most stable in spleen of Chinese giant salamander treated with LPS or GSIV. Therefore, the result showed that GAPDH was the suitable reference gene in different tissues and at different developmental stages of Chinese giant salamander. The β‐actin could be used as a reference gene in spleen of Chinese giant salamander challenged with LPS and GSIV. This study provides convincing information for the GAPDH and β‐actin as suitable reference gene in Chinese giant salamander of different tissues, different developmental stages and different challenges respectively.  相似文献   

Pathogenicity of halophilic Vibrio harveyi in giant freshwater prawns (Macrobrachium rosenbergii De Man)     

Wilailak Siripornadulsil  Mutjarin Thongserm  Surasak Siripornadulsil 《Aquaculture Research》2014,45(12):1979-1988

In this study, the phenotypic and pathogenic properties in freshwater host were characterized in 14 strains of halophilic Vibrio harveyi isolated from infected marine black tiger shrimp, Penaeus monodon. The lysogenic phenotype was assayed via prophage excision and mitomycin C induction. The bacteria were grouped into two types, corresponding to lysogenic and non‐lysogenic strains. The pathogenicity was determined via direct injection of bacterial cultures into post‐larval juvenile giant freshwater prawns, Macrobrachium rosenbergii De Man. All of the infected prawns showed similar symptoms and inflamed hepatopancreas. The V. harveyi isolates derived from the first‐injected infected prawns were re‐isolated and re‐injected into healthy giant freshwater prawns, in which they retained similar infectivity. Both lysogenic and non‐lysogenic Vibrio spp. showed identical virulence associated with 100% mortality within one day post‐injection. TEM micrographs showed hepatopancreatic nuclear deformation and lipid breakdown caused by lysogenic γ‐hemolytic VL19 and non‐lysogenic β‐hemolytic V33. However, the V33 strain was associated with severely disrupted mitochondria. None of the V. harveyi strains was able to produce a biofilm. Together, our findings indicate that the lysogenic and non‐lysogenic halophilic V. harveyi isolated from marine shrimps may use different virulence factors that are responsible for their pathogenicity in freshwater prawns.  相似文献   

大鲵虹彩病毒TaqMan实时荧光定量PCR检测方法的建立   总被引：2，自引：2，他引：2  

周勇  曾令兵  孟彦  周群兰  张辉  高正勇  肖艺  孙建滨 《水产学报》2012,36(5):772-778

利用PCR技术扩增出大鲵虹彩病毒(giant salamander iridovirus, GSIV)主要衣壳蛋白(MCP)编码区长度为1 392 bp 的片段, 克隆到 pMD19-T载体上, 构建重组质粒 pMD19-T-MCP。经PCR鉴定确认正确后, 以10倍梯度稀释 pMD19-T-MCP重组质粒, 作为标准模板进行 TaqMan实时荧光定量PCR扩增, 制作标准曲线, 建立了大鲵虹彩病毒的 TaqMan实时荧光定量PCR检测方法。制作的标准曲线有极好的线性关系, 且线性范围宽, 相关系数为0.990 19。组内重复试验的CT值标准偏差为0.52%。检测结果显示, 该方法对大鲵虹彩病毒的检测有高度的特异性, 与锦鲤疱疹病毒、弗氏柠檬酸杆菌、嗜水气单胞菌以及鲤上皮瘤细胞基因组DNA之间均无交叉反应, 特异性好, 检测总DNA灵敏度为10个病毒核酸分子拷贝数, 约1.1×10-3 pg/μL病毒核酸, 较之常规PCR的敏感度高出约1 000倍。研究建立的大鲵虹彩病毒TaqMan实时荧光定量PCR方法灵敏度高、特异性强, 对大鲵虹彩病毒病的快速诊断与病毒病原定量检测有重要意义。  相似文献   

鞍带石斑鱼仔稚（幼）鱼的发育和生长研究   总被引：7，自引：0，他引：7  

郭仁湘  符书源  杨薇  陈国华  骆剑  刘扬潮  周先雄 《水产养殖》2011,32(4):8-13

对鞍带石斑鱼的仔稚幼鱼形态发育的各个阶段进行了观察与研究,详细描述从初孵仔鱼到幼鱼各个发育时期的形态特征和发育时间。根据卵黄囊的变化,长鳍棘的长出与收缩,鳞片和体色斑纹的出现,鞍带石斑鱼胚后发育可以划分为仔鱼期、稚鱼期、幼鱼期。仔鱼期又可分为卵黄囊期仔鱼和后期仔鱼。水温27～30℃,盐度27～31,pH值8.0～8.4的海水中培育,初孵仔鱼至孵化后2日龄为卵黄囊期仔鱼。2日龄仔鱼开口,3日龄至20日龄为后期仔鱼,22日龄至30日龄为稚鱼期,31日龄进入幼鱼期。鞍带石斑鱼胚后发育过程中最明显的变化是背鳍棘和腹鳍棘的生长和收缩,也是生产育苗当中比较关键的仔稚幼鱼变态过程。  相似文献