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应用还原和非还原单向SDS-PAGE及二者结合的双向电泳技术,研究了红苋R104种子谷蛋白的亚基组成。谷蛋白由多种亚基组成,高分子量二硫键连接蛋白占其多数,经还原裂解成A(54KD)、B(40KD,39KD)、C(33KD,31KD)、D(22KD,20KD,18KD,)和E(15KD)5组主要单肽链单元,提出了二硫键连接蛋白的组成模式,并发现低分子量谷蛋白亚基存在肽内二硫键 相似文献
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种子自然老化时蛋白质类型的变化 总被引:1,自引:1,他引:1
许多作物种子,在收获后的贮藏过程中,往往使活力下降或丧失种子活力,既影响了农业生产又降低了人们食用的价值。由于种子活力的重要性,吸引不少研究者从不同的角度进行研究,目前获得了许多与种子活力有关的生理生化指标。但是影响种子活力的一个重要方面 相似文献
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两个低谷蛋白基因插入缺失标记的设计与验证 总被引:2,自引:0,他引:2
低谷蛋白稻米是肾脏病人极有效的食疗辅助品,培育低谷蛋白功能性水稻品种具有重大意义。低谷蛋白基因Lgc1作为培育低谷蛋白功能性水稻品种的优质资源,受到了育种家的青睐。为提高低谷蛋白基因Lgc1分子标记辅助选择的准确性,我们根据其突变体在两个谷蛋白基因GluB4和GluB5间的碱基缺失,设计出了Lgc1基因的插入缺失标记InDel-Lgc1-A和InDel-Lgc1-B。利用InDel标记对W3660(Lgc1低谷蛋白品种)/南粳46(谷蛋白正常品种)的F2分离群体和13份水稻品种进行检测验证。依据其PCR扩增产物的电泳带型,可准确地区分出低谷蛋白纯合基因、谷蛋白正常纯合基因和杂合基因型3种带型,且3种带型与其植株或品种相应的蛋白性状表现完全一致,表明这两对InDel标记可用于Lgc1低谷蛋白基因资源的鉴定以及分子辅助育种。 相似文献
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小麦高分子量谷蛋白1Dx5亚基特异PCR标记 总被引:2,自引:0,他引:2
本研究为1Dx5亚基基因(Glu—Dl-1b)设计了一对特异引物,对HMW—GS在Glu—Dx1位点已知的11个小麦品种进行了PCR扩增,以检测PCR标记的准确性。结果表明,凡具有1Dx5亚基的4个品种都能扩增出一条450bp的特异带,而其它品种则不能扩增出这一特异带.与HMW—GS的蛋白质电泳结果一致。用这一特异标记对山东省推广种植面积较大的35个品种进行PCR检测.发现仅有9个品种携带1Dx5基因,占待测材料总数的25.7%,明显低于北美小麦品种。研究发现,与蛋白质的HMW—GS标记相比.PCR标记更快速、简便、准确。 相似文献
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通过对染色体工程法培育出的小麦新品种M8003及其亲本种子的麦谷蛋白进行SDS—PAGE分析,结果表明:M8003麦谷蛋白的绝大部分亚基组成与其回交亲本“中国春”相似,而其第6,7,8三个亚基条带与非回交亲本奥地利黑麦的第5,6,7三个亚基条带带型相同,说明M8003麦谷蛋白的6,7,8三种亚基是来自奥地利黑麦染色体基因编码的,进一步证明了M8003属于小麦—黑麦异易位系的细胞学鉴定结果。 相似文献
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Screening of Rice Varieties for Endosperm Storage Proteins 总被引:2,自引:0,他引:2
A total of 118 rice varieties/lines (Oryza sativa L.) from Bangladesh were analyzed for endosperm storage proteins on a single seed basis. The screening was done by observing the profiles of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with standard japonica variety ‘Kinmaze’. Six groups were classified on the basis of the poly-peptide banding patterns. Two-dimensional gel electrophoresis was performed to ensure the SDS-PAGE results in selected varieties. The analysis of extracted protein fractions revealed that one variety had low prolamin and one variety had high glutelin content. 相似文献
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BT型杂交粳稻育性及其三系的若干蛋白质标记 总被引:6,自引:0,他引:6
用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)方法,对粳稻BT 细胞质雄性不育系六千辛A,保持系六千辛B,恢复系六千辛R、77302-1,以及杂交组合六千辛A/77302-1的F1和F2 种子的胚乳贮藏蛋白进行了分析。结果表明,在谷蛋白α3区域,恢复系有两条带α3a 和α3b,而六千辛A和六千辛B只有一条带α3。 F2代具有α3的种子和具有α3a 加α3b的种子1∶1分离。谷蛋白α4带的移动速率,恢复系比六千辛A快。把较快的α4带记为α4f。 F2代具有α4的种子和具有α4 加α4f的种子也是1∶1分离,与配子体不育类型的F2代花粉育性恢复基因分离比一致。系谱分析表明六千辛R中α3a 和α3b来源于IR8。六千辛A比六千辛B容易提取醇溶蛋白。 相似文献
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Tomoyuki Katsube-Tanaka Nadar Khan Satoru Yamaguchi Takeshi Yamaguchi Shuichi Iida 《Plant Production Science》2016,19(3):401-410
Multigenic glutelins and monogenic globulin are major storage proteins accumulating in vacuole-derived protein body (PB-II) of rice (Oryza sativa L.) seeds. Because their interplay in PB-II formation was scarcely known, the effect of globulin-less mutation on glutelin accumulation was investigated. In globulin-less mutants, no phenotypic defect was found in seed and plant growth, while PB-II was deformed and apparent glutelin composition was changed, producing new glutelin α polypeptides X1–X5. 2D-PAGE of different combinations of globulin-less and glutelin subunit mutations suggested that the X1/X2, X3, and X4/X5 were derived from glutelin GluB1/GluB2/GluB4, GluA3, and GluA1/GluA2 subunits, respectively. Western blot with glutelin GluB4 subunit-specific and its variable region discriminable antibodies indicated at least in part the new spots X1/X2 are partially degraded products of GluB4 α polypeptides by the removal of 2–39 residues from C-terminus. Time course experiments with maturing seeds indicated the partial degradation of GluB4 occurred earlier (from 7 days after flowering) and higher than that of GluA1/GluA2. Considering the above results together with the fact that globulin accumulates at the periphery of PB-II and its absence produces deformed PB-II, globulin protects glutelins from proteinase digestion and thereby facilitates stable glutelin accumulation. 相似文献
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