Screening for Small Molecule Inhibitors of BMP-Induced Osteoblastic Differentiation from Indonesian Marine Invertebrates     

Hiroyuki Yamazaki  Satoshi Ohte  Henki Rotinsulu  Defny S. Wewengkang  Deiske A. Sumilat  Delfly B. Abdjul  Wilmar Maarisit  Magie M. Kapojos  Michio Namikoshi  Takenobu Katagiri  Hiroshi Tomoda  Ryuji Uchida 《Marine drugs》2020,18(12)

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马尾松叶表微生物多样性     

袁贵云  孙学广  郑炀  郭其强  丁贵杰 《森林与环境学报》2021,(3):318-324

为发掘对林木生长发育有利的优良微生物资源,并筛选适合叶表微生物的收集方法,以马尾松针叶为试验材料,分别用悬摇法和超声波法收集马尾松叶表微生物,用扩增子高通量测序技术、MUSCLE和Qiime软件研究马尾松叶表微生物的多样性。结果表明:扫描电镜观测结果显示,马尾松针叶表面定殖有大量微生物,包括真菌(菌丝及孢子)和细菌。扩增子高通量测序结果表明,马尾松叶表微生物物种丰富,包含细菌运算分类单位(OTUs)490个,真菌OTUs 1273个。马尾松叶表细菌以未分类的蓝细菌属(unidentified_Cyanobacteria)(36.53%)、未分类的拜叶林克氏菌属(unidentified_Beijerinckia)(28.60%)、鞘氨醇单胞菌属(Sphingomonas)(2.35%)为优势属;叶表真菌以枝孢属(Cladosporium)(2.45%)、拟盘多毛孢属(Pestalotiopsis)(0.92%)、无头孢菌属(Capnobotryella)(0.91%)为优势属。针对叶表细菌多样性的研究表明,悬摇法和超声波法均有较高的物种检出度;在叶表真菌多样性的研究中超声波法优于悬摇法,但超声波法样品间数据变异性较大,测定结果不稳定。  相似文献   

基于BP神经网络的人机联合率定SWMM研究     

桂晗亮  张春萍  武治国 《中国农村水利水电》2021,(3)

为解决雨洪管理模型(SWMM)在率定过程中参数复杂、过程繁琐等问题。以西宁市某地块为例建立SWMM模型,利用Morris筛选法进行参数灵敏度分析,并根据灵敏度分析的结果进行人工率定;另外利用BP神经网络对模型进行率定,并结合参数灵敏度对其进行优化。对3种率定方案进行分析,结果表明:水文水力模块参数的相对灵敏度基本一致,其中灵敏度较大的参数为子汇水区面积(Area)、不透水率(Imperv)和不透水区洼地蓄积量(Destore-Imperv),并且不同降雨条件下模型参数的灵敏度存在差异。经过优化后的BP神经网络参数率定方法的模型模拟效果最好,纳什系数最大。结合灵敏度优化BP神经网络的人机联合率定方法一方面能提高BP神经网络率定的准确性,另一方面又能提高传统人工率定的效率。  相似文献   

苹果花脸病田间病情发展及ASSVd在组培条件下的传播     

郗娜娜  赵坷  杨金凤  孟祥龙  胡同乐  王树桐  王亚南  曹克强 《园艺学报》2020,47(12):2397-2404

为了明确苹果锈果类病毒（apple scar skin viroid，ASSVd）引起的苹果花脸病在田间的病情发展情况以及ASSVd在组培条件下的传播方式，2012—2015年对河北省顺平南神南村3个果园进行持续调查和检测。结果显示，果园A、B、C的显症率分别从3.00%、19.35%和10.00%上升至10.00%、34.41%和22.00%，带毒率分别从11.00%、20.43%和14.00%上升至23.00%、37.63%和30.00%，带毒率和显症率都逐年增加，而且带毒率大于显症率，即有些带毒果树不显症。发病果树田间分布有明显的发病中心。通过高通量测序发现显症果树ASSVd vsiRNA reads数是带毒未显症reads数的4.08倍，同时实时荧光定量PCR检测发现显症树ASSVd病毒积累量显著高于带毒未显症树。以携带ASSVd的组培苗和无毒组培苗为试材，利用RT-PCR检测明确了ASSVd可通过伤口沾染带毒汁液、组培剪污染、含毒培养基、根系接触进行传播，其中根系接触传染率较高。  相似文献   

正交设计优化青蒿SRAP-PCR反应体系及引物筛选     

石紫薇  梁妍  姜婷婷  梁剑平  贾宁  辛志君  陆锡宏  周翔  李雪虎 《分子植物育种》2020,(10):3303-3310

为了建立青蒿的SRAP最佳扩增体系,并筛选出SRAP多态性引物,本研究以青蒿叶片DNA为模板,采用正交试验设计,以Mg^2+、dNTP Mix、Taq DNA聚合酶、引物和DNA模板5种因素5个水平,对青蒿SRAP反应体系进行研究。结果表明,青蒿SRAP-PCR最佳反应体系为:引物0.6μmol/L、Mg^2+2.0 mmol/L、模板DNA 5.1 ng、Taq DNA聚合酶2.0 U、dNTPs 0.25 mmol/L,总体积为25μL。各因素对扩增反应均有不同影响,其中引物浓度的影响最大,dNTPs的影响最小。运用该体系对不同种质资源的青蒿进行验证,证明该体系稳定可靠,并在30个引物组合中筛选出了25对扩增条带清晰,多态性丰富的引物组合。这一结论为今后利用SRAP标记技术进行青蒿分子遗传学研究提供了科学依据。  相似文献   

The role of ear environment in postharvest susceptibility of maize to toxigenic Aspergillus flavus     

Samuel K. Mutiga  Nelson Chepkwony  Owens A. Hoekenga  Sherry A. Flint‐Garcia  Rebecca J. Nelson 《Plant Breeding》2019,138(1):38-50

A kernel screening assay (KSA) was used to assess the genetic and environmental effects on the vulnerability of maize to aflatoxin accumulation. Kernels of 26 inbred lines that had been grown in seven environments, and 190 lines of the Intermated B73xMo17 (IBM) population grown in one location in the United States, were inoculated with a toxigenic strain of A. flavus and incubated in the dark at 30°C for 6 days. Percent kernel colonization (PKC), sporulation and aflatoxin were influenced by the maize genotypes (G), the location (“ear environment” or E) and the GxE interactions. Overall, low broad‐sense heritabilities were observed for PKC, sporulation and aflatoxin. PKC was significantly correlated with sporulation in all environments. Aflatoxin was positively correlated with colonization for two and with sporulation for all ear environments. Higher grain sulphur or magnesium in IBM was associated with less colonization or aflatoxin. Postharvest susceptibility of maize to aflatoxin is thus influenced by factors that are modulated by the ear environment. In a KSA, sporulation could be a proxy test for aflatoxin accumulation.  相似文献   

猪带绦虫六钩蚴cDNA文库的构建与筛选   总被引：1，自引：0，他引：1  

唐雨德  陆承平 《中国兽医学报》2003,23(6):567-569

在体外将猪带绦虫虫卵孵化为有活力的六钩蚴。采用商品化试剂盒抽提六钩蚴总 RNA、m RNA,反转录为双链c DNA,再加 Eco R Adapter。在去除小片段后 ,dsc DNA与 λgt11噬菌体 DNA连接 ,经蛋白包装 ,构建了猪带绦虫六钩蚴λgt11c DNA文库。该文库效价为 3.5× 10 9pfu/ m L ,重组 DNA片段大小为 0 .5～ 3.2 kb,平均为 1.6 kb,蓝白斑比 1∶ 9。用抗体探针对文库进行免疫学筛选 ,在消除非特异性反应的基础上筛选约 10 6 重组子 ,共得到 118株强阳性克隆 ;应用 PCR鉴定上述部分阳性克隆 ,均扩增到 0 .5 kb以上的片段。结果显示 ,构建的文库合格 ,含六钩蚴所有抗原基因 ,可用于六钩蚴 c DNA克隆的筛选 ;用免疫学与 PCR联合筛选 c DNA文库 ,可消除假阳性  相似文献   

Rapid Screening for Aluminum Tolerance in Cereals by Use of the Chlorophyll Fluorescence Test   总被引：1，自引：0，他引：1  

M. Moustakas    G. Ouzounidou  R. Lannoye 《Plant Breeding》1993,111(4):343-346

The effects of aluminum on the photosynthetic apparatus were examined in cereals grown in nutrient solutions (pH 4.5) at two Al levels (0 and 148 μM). The chlorophyll fluorescence kinetics results confirmed that the soft wheat ‘BHG’ cultivar has the potential for growth on acid soils while triticale cultivars ‘Niovi’ and ‘Dada’ appeared to be relatively tolerant. The percentage decrease in F_v/F_m of the less tolerant cultivars after Al-treatment indicated a decrease in the efficiency of the primary photochemistry of PS II, while the decrease in the ratio F_V/F_osuggested that exposure of the cultivars ‘Dio’ and ‘Appulo E’ to aluminum caused injury to the thylakoid structure. The percentage fluctuations of the ratio F_v/F_mwere shown to correlate very closely with the assessment of injury as evaluated by the relative top fresh weight. Measurements of chlorophyll fluorescence in vivo could be used to monitor injury caused by “Al-stress”, and thus they may serve as a rapid screening test for Al tolerance.  相似文献   

Development of a semi-selective medium and an immunofluorescence colony-staining procedure for the detection of Clavibacter michiganensis subsp. sepedonicus in cattle manure slurry     

N. J. M. Roozen  J. W. L. Van Vuurde 《European journal of plant pathology / European Foundation for Plant Pathology》1991,97(5):321-334

Various compounds and basal media were tested for their suitability to create a semi-selective medium for isolation ofClavibacter michiganensis subsp.sepedonicus (Cms) from cattle manure slurry containing c. 10⁸ colony forming units (cfu) per ml.Plating efficiency of Cms in yeast glucose mineral medium (YGM) was 104% compared with yeast peptone glucose medium. Nalidixic acid, polymyxin B sulphate and the experimental disinfectant S-0208 inhibited colony growth of cattle slurry bacteria as compared with Cms in YGM. The optimal concentration of these inhibitors in combination was determined by modified agar diffusion tests and by pour plating in 24-well tissue culture plates. The semi-selective medium YGMI consisted of YGM supplemented with nalidixic acid (2 mg/l), polymyxin B sulphate (30 mg/l) and S-0208 (125 mg/l). Plating efficiency varied for Cms between 50.9 and 69.6%, for cattle slurry bacteria between 1.8 and 2.5% and for saprophytes from potato heel end extracts between 11.5 and 27.4%.Differentiation of Cms colonies from other colonies was based on their small and bluish colony morphology in pour plates and on immunofluorescence colony-staining (IFC). IFC of a pure culture of micro colonies of Cms in YGM was possible after one day incubation (colonies c. 5 cells). Green background fluorescence in the agar gels was prevented by addition of Tween 20 (0.1%) to the washing buffer and the use of 1% agar gels. IFC of macro colonies of Cms in YGMI, visible with 4x objective magnification, was possible after 4 days. The detection level of the target organism in artificially inoculated cattle slurry in YGMI based on colony morphology varied between 1.4×10³ and 2.3×10⁴ cfu per ml of cattle slurry. Miniaturized plating combined with IFC, using wells in tissue culture plates (=16 mm), proved suitable for detection, but was c. 30 times les sensitive. The recovery of Cms was negatively correlated with the number of saprophytic colonies in the agar plates (R ²=0.74).  相似文献   

关于编制我国果树种质资源评价系统若干问题的商榷   总被引：17，自引：3，他引：14  

景士西 《园艺学报》1993,20(4):353-357

资源评价是果树种质资源工作的中心环节。编制果树种资源评价系统是发展和提高我国果树种质资源工作水平的一项重要基础工作。“七五”期间,“果树种质资源主要性状鉴定评价”专题组编制１８种果树的《果树种质资源描述符》,于１９９０年在农业出版社出版。本文就其中其中若干问题提出商榷意见。  相似文献