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地膜覆盖对棉枯萎病发生的影响 总被引:1,自引:0,他引:1
地膜覆盖显著降低了棉花枯萎病的发病率与病情指数,极显著地提高了棉花的产量。 相似文献
5.
上海松江雷竹引种拓植初报 总被引:1,自引:0,他引:1
从母竹质量、生态条件及种植技术3个方面,对雷竹引种成活率进行较系统的研究,结果表明,在平原水网地区的生态条件下,应按竹子的年龄、胸径、高度、枝下高、留枝盘数5个品质指标选用母竹;将种植地化整为零,建成1500m~2的生产小区,使沟渠道路配套搞好排灌措施,降低地下水位,弥补雨量偏少之不足,采用相应的种植技术,使母竹与生态环境统一,从而保证平原水网地区雷竹引种获得成功。 相似文献
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加快淮北地区杂交粳稻发展的几个问题 总被引:2,自引:0,他引:2
回顾了淮北地区十多年来杂交粳稻示范推广的发展过程,分析了影响杂交粳稻发展的主要原因,并论述了加快该地区杂交粳稻发展的几个主要问题。 相似文献
8.
Hideki OGISO Masashi FUJINAGA Hideki SAITO Toshiaki TAKEHARA Shigeru YAMANAKA 《Journal of General Plant Pathology》2002,68(4):292-299
One hundred and sixteen isolates of Fusarium oxysporum f. sp. lactucae obtained from 85 fields in three crisphead lettuce-producing areas in Nagano Prefecture, Japan were typed for races using
differential cultivars Patriot, Banchu Red Fire and Costa Rica No. 4. They were also grouped into vegetative compatibility
groups (VCGs) using complementation tests with nitrate non-utilizing (nit) mutants. Two California strains reported as F. oxysporum f. sp. lactucum, a type culture of F. oxysporum f. sp. lactucae, and 28 avirulent isolates of F. oxysporum obtained from crisphead lettuce were included for comparison. Among Nagano isolates, 66 isolates were identified as race 1,
and 50 as race 2. Race 1 strains derived from Shiojiri and Komoro cities and race 2 from Kawakami village and Komoro city.
All isolates of race 2 were biotin auxotrophs, and the race could be distinguished based on its requirement for biotin on
minimal nitrate agar medium (MM). Pathogenic isolates were classified into two VCGs and three heterokaryon self-incompatible
isolates. Strong correlations were found between race and VCG. All the race 1 strains were assigned to VCG 1 except self-incompatible
isolates, and all the race 2 strains to VCG 2. The 28 avirulent isolates of F. oxysporum were incompatible with VCG 1 and VCG 2. California strains was vegetatively compatible with VCG 1, and they were assigned
to race 1. Based on vegetative compatibility, these two races of F. oxysporum f. sp. lactucae may be genetically distinct, and F. oxysporum f. sp. lactucae race 1 is identical to F. oxysporum f. sp. lactucum.
Received 7 May 2002/ Accepted in revised form 6 September 2002 相似文献
9.
Motoo KOITABASHI Masataka IWANO Seiya TSUSHIMA 《Journal of General Plant Pathology》2002,68(2):183-188
Microorganisms isolated from wheat leaf surfaces were screened for inhibition of wheat powdery mildew. A new screening method,
in which wheat leaves were inoculated with Blumeria graminis f. sp. tritici and incubated with the cultured microorganisms under non-contact conditions, was developed in the present study. Using this
method, 10 phylloplane fungi that inhibited wheat powdery mildew were selected from 408 microorganisms isolated from wheat
leaf surfaces. Among these 10 strains, a fungus designated as Kyu-W63 had an especially strong inhibitory effect. Kyu-W63
produced white colonies without spores when cultivated on PDA. Kyu-W63 had a strong aromatic odor when being cultured. Wheat
powdery mildew was suppressed even though a membrane filter with a pore size of 0.45 μm was placed between the mycelial colony
and wheat leaf segment. However, when activated charcoal was introduced, Kyu-W63 did not inhibit growth of B. graminis. It was presumed that volatile substances were involved in the inhibitory effect of Kyu-W63. GC-MS analysis was used to identify
two substances produced by Kyu-W63 with molecular weights of 164 and 166. Kyu-W63 also inhibited the in vitro growth of four plant pathogenic fungi other than B. graminis.
Received 19 September 2001/ Accepted in revised form 7 February 2002 相似文献
10.
Carmen García-Limones Ana Hervs Juan A. Navas-Corts Rafael M. Jimnez-Díaz Manuel Tena 《Physiological and Molecular Plant Pathology》2002,61(6):325
To ascertain if active oxygen species play a role in fusarium wilt of chickpea caused by Fusarium oxysporum f. sp. ciceris, the degree of lipid peroxidation (malondialdehyde formation) and the activity levels of diamine oxidase (DAO), an apoplastic H2O2-forming oxidase, and several antioxidant enzymes, namely ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), guaiacol-dependent peroxidase (GPX) and superoxide dismutase (SOD), were determined spectrophotometrically in roots and stems of ‘WR315’ (resistant) and ‘JG62’ (susceptible) chickpea cultivars inoculated with the highly virulent race 5 of the pathogen. Moreover, APX, CAT, GPX and SOD were also analysed in roots and stems by gel electrophoresis and activity staining; and the protein levels of APX and SOD in roots were determined by Western blotting. In roots, infection by the pathogen increased lipid peroxidation and CAT and SOD activities, although such responses occurred earlier in the incompatible compared with the compatible interactions. APX, GPX and GR activities were also increased in infected roots, but only in the compatible interaction. In stems, infection by the pathogen increased lipid peroxidation and APX, CAT, SOD and GPX activities only in the compatible interaction, and DAO activity only in the incompatible one. In general, electrophoregrams agreed with the activity levels determined spectrophotometrically and did not reveal any differences in isoenzyme patterns between cultivars or between infected and non-infected plants. Further, Western blots revealed an increase in the root protein levels of APX in the compatible interaction and in those of SOD in both compatible and incompatible interactions. In conclusion, whereas enhanced DAO activity in stems, and earlier increases in lipid peroxidation and CAT and SOD activities in roots, can be associated with resistance to fusarium wilt in chickpea, the induction of the latter three parameters in roots and stems along with that of APX, GR (only in roots) and GPX (only in stems) activities are rather more associated with the establishment of the compatible interaction. 相似文献