Inhibitory Effects of AURKB Gene on Apoptosis and Cancer Cell Growth in HCT 116 Cells     

Wangyuan ZENG  Wenxin DAI 《Medicinal Plant》2019,(6):77-86

[Objectives]To explore the inhibitory effect of AURKB gene in apoptosis and cancer cell growth in HCT 116 cells.[Methods]The in vitro cytology studies were carr...  相似文献   

Performance Evaluation of the ADS 1000 Ventilator Using a Lung Model     

L.S. Faudskar DVM  M. R. Raffe DVM  MS  D. A. Randall BS  D. R. Bing BS  RRT 《Journal of Veterinary Emergency and Critical Care》1997,7(1):49-58

A laboratory evaluation was performed to evaluate the performance characteristics of a new veterinary ventilator. The ventilator studied was configured according to manufacturer's directions and attached to a test lung via a pneumotachograph and differential pressure transducer interfaced to a pulmonary mechanics analyzer system. Constant resistance (R=10 cm H₂O/L/sec) and compliance (C=3 ml/cm H₂O) factors were maintained for all trials. The ventilator operated at the manufacturer's preprogrammed parameters. In the first trial, body weight was the only variable. In the second trial, an endotracheal tube was placed in series between the ventilator's breathing circuit and the pneumotachograph. Body weights from 1–20 kgs were evaluated. Mean values for respiratory rate (RR), minute ventilation (VE), inspiratory time (Ti), peak inspiratory pressure (PIP), and peak inspiratory flow (Fpki) displayed on the ventilator control panel; tidal volume (VT), calculated from the displayed minute volume, and identical parameters measured by the pulmonary mechanics system at each body weight, were compared using a two factor analysis of variance. Significant differences (P< 0.05) were found between mean displayed and measured values for RR, PIP, and Fpki.  相似文献   

Permeability of the endothelium and partitioning of the pulmonary blood flow resistance in isolated perfused pig lungs: Effects of breed and age     

P. Gustin  B. Urbain  A. Delaunois  K. Zeimes  M. Ansay 《Veterinary research communications》1992,16(1):69-82

The right and left lungs of 5 healthy Minipigs and of 13 healthy Landrace piglets were isolated, perfused at constant pressure and maintained in an isogravimetric state under zone III conditions (pulmonary venous pressure>alveolar pressure). By applying the double, arterial and venous, occlusion technique, the total blood flow resistance (R _t) was partitioned into four components: arterial (R _a), pre-(R _a) and post-capillary (R _v) and venous (R _v). The capillary filtration coefficient (K _f,c) was evaluated by measuring the weight gained by the lungs when the arterial and venous pressures were suddenly increased. In the youngest Landrace piglets (5 weeks old), there was an uncontrolled vasoconstriction which sometimes prevented perfusion of the lungs and induced a large increase inR _t. These high values ofR _t were decreased by tolazoline administration. The values ofR _t recorded in older pigs (12–13 weeks old) were lower in Minipigs (33.66±3.77 cmH₂O min L^–1 per 100 g of lungs;n=5) than in Landrace piglets (55.20±6.18 cmH₂O min L^–1 per 100 g;n=5). This breed difference was due to the differences inR _a andR _v. The mean values ofK _f,c were 0.193±0.015 and 0.202±0.029 ml min (cmH₂O)^–1 per 100 g of the lungs in Minipigs and Landrace piglets respectively. All these parameters were stable for the 3 hours following the equilibrium period. It was concluded that: (1) There is an age-related maturation of the control of the vasomotor tone in porcine lungs. (2) Pulmonary microvascular haemodynamics are influenced by the breed of the pigs. (3) There was no difference in theK _f,c values between both the breeds. (4) A comparison of the values reported for dogs and rabbits with our data shows that the pre- and post-capillary resistances and, to a lesser extent, the arterial and venous resistances are relatively high in pigs.  相似文献   

高+Gx过载作用对恒河猴肺脏的影响     

郝俊峰  吴斌  赵德明  宁章勇  王传武  秦秀慧 《中国兽医学报》2005,25(4):400-402

15只健康恒河猴于清醒状态下，在动物离心机上经受相应峰值( 1Gx、 15Gx、 18Gx、 21Gx)的抛物线型过载作用后，按要求在过载后不同时期剖解，大体观察、取材，进行病理形态学的定性研究。结果显示：(1)眼观病变。 15Gx组、 18Gx组和 21Gx组在过载作用后即刻肺脏出现了不同程度的气肿、萎陷、淤血和出血点或斑；恢复组可见肺脏边缘有不同程度气肿区域，肺脏的背面呈暗红色；在各叶的背面均可见大小不等、多少不一的暗红色出血点或斑。(2)光镜下可见各急性实验组动物的肺脏呈现不同程度的气肿区和萎陷区，肺泡壁毛细血管扩张充血，肺泡腔内有浆液和红细胞渗出； 21Gx组还出现了血管内溶血、细支气管粘膜脱落和出血等，肺脏的病理损伤随G值升高而明显加重； 15Gx作用造成的肺脏损伤在1个月后基本恢复。而 21Gx作用后1个月肺脏的损伤尚未恢复，且出现了白细胞浸润、浆液渗出和增生等炎症反应。因此，高 Gx过载可引起猴肺脏明显的病理性损伤，其中 15Gx造成的损伤相对较轻，且容易恢复，而 21Gx造成的损伤严重，且难以恢复。  相似文献   

山羊胎期肺发育的形态学研究     

郑月茂  徐永平  卿素珠  蒲鹏  张涌 《畜牧兽医学报》2005,36(2):158-165

对3～22周龄山羊胎儿肺进行了肉眼、光镜和透射电镜观察，结果表明：1．7～22周龄山羊胎儿肺的外部形态与胎龄无关，肺的外部形态以左二右四叶者为多见．肺的叶间裂和右肺副裂常不完整，以浆膜、肺组织或混合性组织(肺组织及浆膜)融合；2．山羊胎儿肺的发育分为5个时期：胚胎期(3～5周)肺芽分支形成主支气管，主支气管长度不断增长并萌芽出叶支气管，均衬以假复层柱状上皮。腺状期(6～12周)以支气管树发育为主，小支气管衬以假复层和／或单层柱状上皮；终蕾呈腺状，上皮细胞由假复层柱状逐渐变为单层柱状，胞核向细胞顶端移行；终蕾上皮细胞游离面可见短小的微绒毛；线粒体、粗面内质网及核糖体随着胎龄增加而逐渐增多，它们均位于细胞顶部。小管期(13～14周)以呼吸部发育为主，原始肺泡开始形成，呼吸性细支气管衬以未分化的立方上皮；终蕾腺状结构逐渐消失，终蕾上皮细胞由高矮不等的单层柱状上皮逐渐演变为立方形的原始肺泡上皮；细胞游离面可见较多的微绒毛，胞质内线粒体、粗面内质网及核糖体较发达。囊状期(第15周)呼吸部发育显著，肺内细支气管及其末端呈现出“充气”状态；部分原始肺泡上皮细胞分化为扁平的肺泡Ⅰ型细胞和立方形的肺泡Ⅱ型细胞；Ⅱ型细胞内出现嗜锇小体。肺泡期(16～22周)以肺泡的形成和分化为主，更多的肺泡上皮分化为扁平的肺泡Ⅰ型细胞和立方形的肺泡Ⅱ型细胞。此期，毛细血管内皮与部分肺泡上皮贴近，可将肺泡上皮细胞区分为3种：Ⅰ型细胞，呈矮柱状或椭圆形，胞质中有较明显的核糖体、扩张内质网及变性线粒体；形成了由Ⅰ型细胞一基膜一内皮细胞组成的气血屏障。Ⅱ型细胞，胞质内含丰富的嗜锇板层小体和核糖体，内质网扩张呈大小不一的泡状，多泡体出现，线粒体膨大变性，细胞游离面可见少数微绒毛。Ⅲ型细胞，为未分化细胞，呈立方形，胞体较小，胞核相对较大，呈圆或椭圆形，胞质少，呈带状．电子密度低，细胞器少。  相似文献   

FGF4在肺癌细胞体内和体外中表达差异的研究     

李江超  袁俏冰  陈卉  黎帅  周泽启  叶宇翔  韩露  王丽京 《广西农业生物科学》2014,(3):501-504

FGF4已经被证明是癌基因,它涉及肿瘤的生长和转移,为了解FGF4的表达与肿瘤微环境的关系。我们利用FGF4抗体通过免疫组化对一名肺癌患者癌旁,癌组织,癌组织小鼠移植瘤,二次移植瘤以及原代培养的细胞爬片进行FGF4检测,探究其表达差异。通过对比癌组织与对照组（癌旁组织）,FGF4在癌巢中高表达;同样,移植瘤与二次移植瘤的癌巢中与癌旁组织比较,FGF4表达相对较高;但是将癌组织进行原代培养后,免疫组化检测细胞爬片FGF4,发现仅有5%±0.21%的肿瘤细胞表达FGF4,对照蛋白Cytokine作为肿瘤标记物,则在100%的肿瘤细胞中表达。研究提示免疫组化检测到FGF4在体内和体外表达不同,提示肿瘤细胞FGF4的表达与肿瘤微环境调控密切相关,肿瘤微环境对肿瘤细胞的FGF4的调控有着重要作用。  相似文献   

EZH2-mediated regulation of NF-κB target gene expression in gastric cancer     

WU Xue-lei  CAI Yao-wu  ZHUANG Zhi-zhong  CHEN Yuan-jing  GUO Ren-jie  ZHENG Mao-song 《园艺学报》2015,31(12):2169-2175

AIM: To explore the mechanism by which over-expression of enhancer of zeste homolog 2 (EZH2) in a panel of gastric cancer cell lines is involved in tumorigenesis of gastric cancer. METHODS: Real-time PCR and Western blot were employed to examine the mRNA and protein levels of EZH2, respectively. MTS assay, cell migration and soft agar assay were performed to investigate the role of EZH2 in the regulation of stomach cancer behaviors. The effect of EZH2 on NF-κB target gene expression was determined by Luciferase reporter and real-time PCR. Co-immunoprecipitation was used to analyze the interaction of EZH2 and p65 in HEK293T cells. RESULTS: The expression levels of EZH2 were significantly increased in the gastric cancer cells compared with normal gastric epithelial cells. Pharmacological inhibition by DZNep or knockdown of EZH2 significantly compromised AGS and SNU-16 cell activity, cell migration and anchorage-independent cell growth. Moreover, siRNA knockdown of EZH2 impaired NF-κB downstream targets, such as IL-8, CXCL5 and CCL20. In addition, the interaction of EZH2 and p65 was detected. CONCLUSION: EZH2 mediates the growth of gastric cancer cells through the regulation of NF-κB downstream gene expression.  相似文献   

Fucoidan and Cancer: A Multifunctional Molecule with Anti-Tumor Potential     

Farzaneh Atashrazm  Ray M. Lowenthal  Gregory M. Woods  Adele F. Holloway  Joanne L. Dickinson 《Marine drugs》2015,13(4):2327-2346

There is a wide variety of cancer types yet, all share some common cellular and molecular behaviors. Most of the chemotherapeutic agents used in cancer treatment are designed to target common deregulated mechanisms within cancer cells. Many healthy tissues are also affected by the cytotoxic effects of these chemical agents. Fucoidan, a natural component of brown seaweed, has anti-cancer activity against various cancer types by targeting key apoptotic molecules. It also has beneficial effects as it can protect against toxicity associated with chemotherapeutic agents and radiation. Thus the synergistic effect of fucoidan with current anti-cancer agents is of considerable interest. This review discusses the mechanisms by which fucoidan retards tumor development, eradicates tumor cells and synergizes with anti-cancer chemotherapeutic agents. Challenges to the development of fucoidan as an anti-cancer agent will also be discussed.  相似文献   

Marine-Derived Secondary Metabolites as Promising Epigenetic Bio-Compounds for Anticancer Therapy     

Mariarosaria Conte  Elisabetta Fontana  Angela Nebbioso  Lucia Altucci 《Marine drugs》2021,19(1)

  相似文献   

Matrix metalloproteinase‐2, ‐7, and ‐9 activities in dogs with idiopathic pulmonary fibrosis compared to healthy dogs and dogs with other respiratory diseases     

Merita Mtt  Henna P. Laurila  Saila Holopainen  Kaisa Aaltonen  Liisa Lilja‐Maula  Sanna Viitanen  Minna M. Rajamki 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2021,35(1):462-471

  相似文献