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1.
Legumes contain a range of non‐nutritional phytochemicals that may have health‐promoting effects in humans. In this study, we determined the concentrations of four phytoestrogens (coumestrol, apigenin, luteolin and quercetin) in field‐grown alfalfa (Medicago sativa L.). Differences between plants of different stages of maturity, between plant parts, and different canopy segments were assessed. The concentration of individual phytoestrogen in whole herbage varied between 15 and 225 μg g?1 dry matter (DM) and was strongly affected by stage of maturity. Coumestrol and apigenin concentrations were highest at early vegetative stages, luteolin and quercetin at early vegetative and late flowering stages. All phytoestrogens were found in lowest concentrations at the early flowering stage (average 68 μg g?1 DM); stage at which alfalfa is usually harvested when used as a forage source for animals. At vegetative stages, apigenin was the predominant phytoestrogen in herbage followed by coumestrol, the reverse being observed upon initiation of flowering; luteolin and quercetin were found at all stages in similarly lower concentrations. Concentrations of luteolin, quercetin and apigenin were 225, 410 and 690 % greater, respectively, in flowers than in leaves or stems; coumestrol concentration was similar between plant parts. In flowers and stems the predominant phytoestrogens were apigenin and quercetin, followed by coumestrol and luteolin. Similar concentrations (average 26 μg g?1 DM) of each of the four phytoestrogens were found in leaves. Concentrations through the herbage canopy varied and were greatest at >60 cm from the soil surface for apigenin and coumestrol, but greatest at >60 and 0–20 cm for quercetin and at 0–20 cm for luteolin. The results suggest that if alfalfa is to be used as a source of phytoestrogens and is harvested for the production of herbal supplements or nutraceuticals, management will need to be adapted. 相似文献
2.
To study the effect of luteolin on blood indexes,liver and kidney in mice with acute mercury poisoning,28 mice were randomly divided into four groups:Control group (intraperitoneal injection 0.9% saline),luteolin group (lavage 100 mg/kg luteolin),mercuric chloride group (intraperitoneal injection 4 mg/kg mercury chloride) and mercury chloride+luteolin group (intraperitoneal injection 4 mg/kg mercuric chloride,lavage 100 mg/kg luteolin).The activities of ALT in serum,AST,CREA and BUN contents,blood WBC,RBC,HGB content and GSH and MDA contents of liver tissue were detected.Morphological changes of liver and kidney tissues were observed.The results showed that compared with the control group,the activities of ALT and AST of mercuric chloride group were extremely significantly increased (P < 0.01),serum CREA,BUN,blood WBC and liver tissue MDA contents were significantly increased (P < 0.05) while blood RBC,HGB and liver tissue GSH contents were significantly decreased (P < 0.05).Liver,kidney pathological changes were obviously.Compared with mercuric chloride group,the activities of ALT,AST in serum,CREA,BUN,blood WBC and liver tissue MDA contents of mercuric chloride+luteolin group were significantly decreased (P < 0.05),while blood RBC,HGB and liver tissue GSH contents were significantly elevated (P < 0.05).Liver,kidney pathological changes were attenuated obviously.The poisoning were characterized by inflammation and the occurrence of anemia when acute mercury poisoning occurred,liver and kidney showed different degrees of injury in mice.Luteolin could reduce the toxic effects of acute mercury poisoning on blood,liver and kidney. 相似文献
3.
本文采用酶法预处理结合超声波辅助提取的方式,最大程度地提高了花生壳总黄酮的得率,并优化大孔树脂纯化工艺,提高了有效成分的纯度。花生壳黄酮的最佳提取工艺为：花生壳粉与水混合,半纤维素酶与木聚糖酶按1:1（m/m）复配,用量0.25‰,50℃酶解30 min后,按料液比1:20（m/V）加入乙醇至终浓度60%,于功率1000 W,55℃超声波辅助提取60 min,花生壳总黄酮的得率约为2.5%。选用D101型大孔树脂,上样缓冲液为pH 5.0的60%乙醇溶液,洗脱液为pH 10.0的70% 乙醇溶液,上样与洗脱流速为0.75 BV/h和1.5 BV/h。纯化后的花生壳总黄酮和木犀草素的纯度分别为10.54%和5.85%,提高了90%和120%。 相似文献
4.
The methanol extract of Ficus sarmentosa var. henryi （King） Corner was found to detect potent inhibitory activities against 6 crop pathogenic fungi, Fusarium graminearum, Pumpkin fusarium Wilt, Curvularia lunata （Wakker） Boed, Septoria zeicola Stout, Botrytis cinerea, and Rhizoctonia solani. Four flavonoids were isolated from the extract of F. sarmentosa var. henryi by activity-guided fractionation and they were identified as eriodictyol （1）, homoeriodictyol （2）, dihydroquercetin （3）, and luteolin （4） by spectroscopic means and displayed excellent inhibitory activity against F. graminearum and S. zeicola. Among them, luteolin （4） showed the strongest inhibitory activity with IC50 values of 56.38 and 81.48 mg L^-1, to the fungi, respectively. The in vitro inhibitory activity of F. sarmentosa var. henryi （King） Corner was reported for the first time and the inhibitory action mechanism of luteolin （4） was worthy of further research. 相似文献
5.
建立了同时测定花生壳中绿原酸、3,4-二咖啡酰奎尼酸和木犀草素3种有效成分含量的反相高效液相色谱方法。采用Kromasil C18柱(200 mm×4.6 mm,5μm),乙腈和0.1%甲酸水溶液梯度洗脱,流速为1.0 mL/min,检测波长340 nm,柱温27℃。在该色谱条件下,绿原酸、3,4-二咖啡酰奎尼酸和木犀草素的质量浓度分别为3.75~60μg/mL(R2=0.999 9),3.63~58.08μg/mL(R2=0.999 9),17.25~276μg/mL(R2=0.999 7)内与色谱峰面积呈现良好的线性关系,平均加标回收率(n=5)为121.6%,97.79%,124.35%,RSD分别为0.71%,1.49%,0.44%。该分析方法快速准确、重现性好,可应用于花生壳资源产业开发中质量标准的建立和完善。 相似文献
6.
JIANG Dai-xun LIU Shu-rong ZHANG Mei-hua ZHANG Tao MA Wen-jing MU Xiang CHEN Wu 《农业科学学报》2015,14(1):140-147
Luteolin is an active ingredient found early from Folium perillae and Flos lonicerae, and has a specific inhibition on phosphodiesterase 4(PDE4) activity in vitro. Researches show luteolin has pharmacological effects of anti-inflammation, anti-anaphylaxis, antitumor, antioxidant, protection of nervous system and so on, and has mainly been used for the treatment of respiratory inflammatory diseases, cancer and cardiovascular disease in clinic. PDE4, specific to hydrolyze cyclic AMP(c AMP), is considered to be a new anti-inflammatory target due to the decisive role on c AMP signal in inflammatory cells such as neutrophils. In order to explore the anti-inflammatory mechanism, we further studied the effects of luteolin on the activity and expression of PDE4, the expression of lymphocyte function-associated antigen-1(LFA-1) and macrophage-1(MAC-1) in neutrophils, and the adhesion of neutrophils and endothelial cells. The results showed that luteolin had a dose-dependent inhibition on both bare PDE4 activity and PDE4 in cultured neutrophils, and had an obviously promotive effect on gene expressions of PDE4 A, 4B and 4D in later period. Luteolin had a significant inhibitory effect on neutrophils adhesion and LFA-1 expression in early stage, and had no obvious effect on MAC-1 expression. Therefore, luteolin can inhibit LFA-1 expression of neutrophils, then inhibit the adhesion of neutrophils and endothelial cells, and the mechanism is at least related with the inhibition of PDE4 activity. 相似文献
7.
试验旨在研究小鼠急性汞中毒后血液、肝脏及肾脏的中毒表现及木犀草素对急性汞中毒小鼠血液指标、肝脏及肾脏的影响。将28只小鼠随机分成4组,分别为对照组(腹腔注射0.9%生理盐水)、木犀草素组(灌胃100 mg/kg木犀草素)、氯化汞组(腹腔注射4 mg/kg氯化汞)和氯化汞+木犀草素组(腹腔注射4 mg/kg氯化汞,灌胃100 mg/kg木犀草素)。检测血清谷丙转氨酶(ALT)和谷草转氨酶(AST)活性,肌酐(CREA)和尿素氮(BUN)水平,血液白细胞(WBC)、红细胞(RBC)和血红蛋白(HGB)水平及肝脏组织谷胱甘肽(GSH)和丙二醛(MDA)水平,并观察肝脏、肾脏组织形态学改变。结果显示,与对照组相比,氯化汞组的血清ALT、AST活性极显著升高(P < 0.01),血清CREA、BUN,血液WBC及肝脏组织MDA含量均显著增加(P < 0.05),血液RBC、HGB及肝脏组织GSH水平均显著降低(P < 0.05),肝脏、肾脏病理变化明显。与氯化汞组相比,氯化汞+木犀草素组的血清ALT、AST活性,CREA和BUN,血液WBC及肝脏组织MDA含量均显著降低(P < 0.05);血液RBC、HGB及肝脏组织GSH含量均显著升高(P < 0.05),肝脏、肾脏病理变化减轻。小鼠急性汞中毒后,其中毒表现为炎症和贫血的发生及肝脏、肾脏不同程度的损伤,木犀草素可减弱急性汞中毒小鼠血液、肝脏和肾脏的毒性作用。 相似文献
8.
建立一种同时测定黄芩苷和木犀草素的高效液相色谱检测方法,并分析菊花中黄芩苷和木犀草素的含量。样品采用超声提取,以0.1%磷酸溶液（A相）-甲醇（B相）为流动相,梯度洗脱程序：0-3 min,30%A～35%A;3-6 min,35%A～40%A;6-9 min,40%A;流速为1.0 mL/min;采用purospher star RP-18（250 mm×4.6 mm,5μm）色谱柱分离;检测波长327 nm;进样量20μL。黄芩苷和木犀草素分别在4.30-43.00 mg/L、5.00-50.00 mg/L范围内线性关系良好（r〉0.999 5）。加标回收率分别为100.2%和98.56%;相对标准偏差为3.2%和1.7%。该方法简便、快速、准确,适用于菊花中黄酮类化合物黄芩苷和木犀草素的检测和含量测定。 相似文献
9.
Mercè Llugany Roser Tolrà Sandra Raquel Martín Charlotte Poschenrieder Juan Barceló 《植物养料与土壤学杂志》2013,176(6):851-858
Glutathione (GSH) and phenolics play an important role in plant defense against metal‐ion toxicity. The antioxidant activity and metal‐binding capacity of these compounds can account for the protective effects. In contrast to animal‐cell models, however, the possible interplay among these substances in stress defense of plants is poorly investigated. This study compares the influence of cadmium (Cd) on the profiles of both soluble phenolics and GSH in shoots of different Thlaspi and Noccaea species: two ecotypes of the nonhyperaccumulator T. arvense differing in Cd resistance (ecotype Aigues Vives, Cd‐sensitive, and ecotype Jena, Cd‐resistant) and two Cd‐tolerant Cd‐Zn hyperaccumulators N. praecox and N. caerulescens (formerly Thlaspi praecox and T. caerulescens). To reveal the possible influence of Cd‐induced sulfur (S) shortage on the stress response, plants receiving normal S concentrations (500 μM MgSO4) and plants treated with surplus S (500 μM MgSO4 + 500 μM K2SO4) were analyzed. Our working hypothesis was that species differences in tolerance to high tissue Cd concentrations should be reflected by differences in endogenous levels of GSH and phenolic compounds. The results reveal clear species‐dependent differences in both the constitutive patterns and the Cd‐ and S‐induced changes in shoot concentrations of GSH and phenolics. However, no simple relationship between these shoot concentrations and Cd accumulation and tolerance can be established. 相似文献
10.
F. M. Hammouda M. M. Seif El-Nasr A. A. Shahat 《Plant foods for human nutrition (Dordrecht, Netherlands)》1993,44(2):163-169
The study of the flavonoids of the leaves ofCynara scolymus cultivated in Egypt (Romanian strain) resulted in the isolation and identification of apigenin-7-O-glucoside, luteolin, cynaroside and scolymoside. 相似文献