Segregation and linkage analysis of DNA markers in microspore derived and F2 populations of oilseed rape (Brassica napus L.)     

P. K. Tanhuanpää  J. P. Vilkki  H. J. Vilkki 《Euphytica》1993,74(1-2):59-65

Summary The segregation of RFLP and RAPD markers was compared in two oilseed rape (Brassica napus L.) breeding populations from the cross Topas x R4, the latter being a low linolenic mutation line. A total progeny of 68 F₂ and 40 microspore derived plants were studied with 25 markers. The results indicated a significant excess of Topas alleles at five RAPD loci in the microspore derived population. This suggests that genomic regions which probably affect microspore culture ability do not have identical distribution in the two population types.  相似文献   

利用游离小孢子培养育成早熟大白菜新品种‘豫新5号’   总被引：5，自引：0，他引：5  

耿建峰  原玉香  张晓伟  蒋武生  韩永平  高睦枪  栗根义 《园艺学报》2003,30(2):249-252

 ‘豫新5号’是采用游离小孢子培养双单倍体育种技术选育的一代杂种，该品种生育期60 d，单球质量23 kg，球形指数1.35，高抗病毒病、霜霉病和软腐病，净菜产量56.7 t·hm^-2，可兼作早熟、晚熟栽培。  相似文献   

芥蓝游离小孢子培养初报   总被引：11，自引：0，他引：11  

何杭军  王晓武  汪炳良 《园艺学报》2004,31(2):239-240

 以芥蓝的5个基因型为试材，对游离小孢子培养诱导胚状体发生及植株再生进行了初步探讨。结果表明：供体基因型对成功诱导胚状体发生至关重要；振荡培养明显有利于胚状体的发育；改良MS和B培养基对诱导植株再生没有差别。  相似文献   

大白菜游离小孢子培养技术高效体系的研究     

李菲  张淑江  章时蕃  张慧  孙日飞  张振贤 《中国蔬菜》2014,1(8):12

以大白菜吉红82、#534DH 群体及#438 群体为试材，研究了利用细胞破碎仪机械提取小孢子与人工挤压提取小孢子对大白菜游离小孢子活力及提取量的影响。结果表明：机械提取替代人工挤压可在短时间内同时提取多份样品，并可快速调整小孢子的培养浓度。收集40 个适期花蕾于5 mL 离心管内，3 000 r·min-1 破碎10 s，将提取的小孢子定容于25 mL NLN-13 培养基，可确保小孢子浓度在适宜培养范围内，并成功诱导胚胎发生。  相似文献   

秋水仙碱处理油菜离体小孢子的染色体加倍效应   总被引：7，自引：1，他引：7  

石淑稳  周永明  吴江生 《华中农业大学学报》2002,21(4):329-333

用秋水仙碱对52份甘蓝型油菜品系（种）和它们的杂种离体小孢子的单倍体二倍化技术进行了研究。从花药中分离出的单核晚期小孢子接种在含10－800mg/L秋水仙碱的NLN液体培养基中处理16－90h后转入无秋水仙碱的相同培养基诱导胚状体。结果表明，用10mg/L秋水仙碱处理小孢子48h，10份材料的双单倍体植株变幅为37.10%-90.12%，平均为65.44%。50mg/L处理48h的8份材料是48.72%-97.81%，平均88.86%。100－800mg/L处理16－48h的试验，多数加倍率在90％－100％之间。但该处理药用量大，费用高，而且对小孢子毒性大，胚状体再生率低。试验还表明，秋水仙碱处理小孢子诱导的二倍体植株所产生的花一般均能结籽，带有不孕花的嵌合植株极少。用秋水碱处理水孢子再生的植株的根或芽所产生的二倍体植株多是可孕和不孕花共生的嵌合植株，自交后产生的单株种子很少，难以达到遗传和育种所需的群体量。  相似文献   

Number of Pollen Grains in Rice Cultivars with Different Cool-Weather Resistance at the Young Microspore Stage     

《Plant Production Science》2013,16(3):299-305

Abstract

The difference in the number of pollen grains may be the primary factor determining the resistance to cool weather at the young microspore stage in rice (Oryza sativa L.) cultivars. To confirm this hypothesis, we compared the number of pollen grains among the cultivars or lines with various cool-weather resistance. The cultivars or lines used in the experiments were mainly released or grown in the Tohoku district (the northern part of the mainland of Japan). The number of pollen grains showed high correlation with the degree of cool-weather resistance, but considerably varied with the cultivar or line, even in the group having the same cool-weather resistance. Each group with different cool-weather resistance was divided into two subgroups, implying the existence of another genetic factor determining the resistance. The number of pollen grains in the plants cooled at the young microspore stage showed higher correlation with the degree of the cool-weather resistance than that in the control plants without exposure to low temperature. The correlation between the pollen number and spikelet sterility was also analyzed using the data of Satake and Shibata (1992, Jpn. J. Crop Sci. 61 :454–462), who primarily used the cultivars in Hokkaido (the northernmost island of Japan). The results were basically the same as those obtained in the present experiments. These results confirmed the hypothesis that the variation in the number of pollen grains is a primary factor of the resistance to cool weather at the booting stage in the cultivars in the northern part of Japan. The results also suggested the existence of another genetic factor determining the resistance.  相似文献   

杏大小孢子与雌雄配子体发育   总被引：7，自引：1，他引：6  

胡宝忠  李桂琴 《东北农业大学学报》1997,28(2):179-185

以龙垦２号品种为试材、分期取样固定，按常规石蜡法制片观察。结果表明，哈尔滨地区３月５日花药壁已分化，次生造孢细胞和花粉母细胞已形成。四分体属同时型，其排列方式为四面体型，成熟花粉呈椭圆形、具３孔沟。有的药室内产生巨型细胞。子房内具２胚珠，１枚后期退化消失。胚珠倒生，近珠孔处为双被，其余部分愈合；厚珠心，具珠心帽结构；胚囊发育属蓼型。杏雌蕊发育的异常情况较其它果树的比率都大。  相似文献   

爬竹雄蕊发育异常的初步观察   总被引：1，自引：0，他引：1  

庞延军  喻富根 《竹子研究汇刊》1994,13(4):42-46

本文报道了爬竹雄蕊发育中有异于正常雄蕊发育过程的现象。观察结果表明,爬竹的某些雄蕊在发育过程中,在不同的时期发生偏离于正常途径的发育,这些异常现象最早出现在雄蕊原基发育时期。异常发育的雄蕊或者不能形成花粉囊;或者形成了花粉囊但囊内细胞高度液泡化,缺乏原生质,无法形成有效的小孢子母细胞。  相似文献   

华山矾大小孢子的发育和雌雄配子体的形成     

徐涛  林养昉  王跃华 《西部林业科学》2002,(4):27-29

研究了华山矾大、小孢子发育和花粉壁的形成以及大孢子的发生和雌配子体的发育过程。华山矾的花药四室。绒毡层腺质型 ,发育后期出现双核至多核。小孢子四分体四面体型 ,胞质分裂连续型。成熟花粉具 2细胞。子房 2室 ,中轴胎座。胚珠倒生 ,单珠被 ,薄珠心 ,胚囊发育为蓼型。该研究为山矾科的胚胎学提供了新资料。  相似文献   

利用游离小孢子培养技术育成早熟大白菜新品种‘豫新60’   总被引：5，自引：0，他引：5  

原玉香  张晓伟  耿建峰  蒋武生  韩永平 《园艺学报》2004,31(5):704-704

 ‘豫新60’系采用游离小孢子培养双单倍体育种技术快速育成的大白菜一代杂种。该品种早熟, 生育期60～65 d ; 高抗病毒病( TuMV) 、霜霉病和软腐病; 净菜产量59.9～69.8 t·hm^-2; 叶球矮桩叠抱, 球形指数1.19 , 单球质量1.5～2.0 kg ; 叶质柔嫩, 生食、熟食皆宜; 口感略甜, 风味佳。  相似文献