小麦4B染色体上LOX基因的等位变异及其区域分布     

王黎明  孔维玮  高华利  董普辉  闫雪芳  王春平  王洪刚  李兴锋 《作物杂志》2021,37(1):32-138

为了解中国不同麦区小麦种质资源籽粒脂肪氧化酶（lipoxygenase,LOX）活性相关基因TaLox-B1的差异和分布,利用小麦4B染色体上的功能标记LOX16和LOX18对7个麦区的436份种质资源进行分子检测。结果表明：在供试材料中共检测到3种TaLox-B1基因等位变异类型,分别为TaLox-B1a（与高LOX活性相关)、TaLox-B1b（与低LOX活性相关）和杂合型,其频率分别为19.0％、70.4％和10.6％。小麦LOX活性基因不同变异类型在各生态区的分布存在明显差异：基因型TaLox-B1a在黄淮冬麦区、北部冬麦区和长江中下游冬麦区分布较多,其比例分别为21.1%、19.8%和17.6%;基因型TaLox-B1b在西南冬麦区和长江中下游冬麦区分布较多,比例分别为87.9%、72.5%;杂合型仅存在于北部冬麦区、黄淮冬麦区与长江中下游冬麦区,比例分别为14.2%、12.4%和9.8%。利用标记LOX16和LOX18对53个自选高代品系进行分子检测,发现自选品系仅有TaLox-B1b与杂合型两种基因型,其中基因型TaLox-B1ab有32个,比例为60.4%。采用分子标记辅助选择,有利于快速鉴定小麦籽粒LOX活性,加速LOX的遗传改良和新品种选育。  相似文献   

FoxA2 and p53 regulate the transcription of HSD17B1 in ovarian granulosa cells of pigs     

Xiaolong Yuan  Xiaofeng Zhou  Xiwu Qiao  Qi Wu  Zhixiang Yao  Yao Jiang  Hao Zhang  Zhe Zhang  Xilong Wang  Jiaqi Li 《Reproduction in domestic animals》2021,56(1):74-82

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枣实蝇对枣果挥发物的选择行为          下载免费PDF全文

梁萌  阿不都瓦哈·艾再孜  阿地力·沙塔尔 《林业科学研究》2020,33(2):145-153

[目的]探究对枣实蝇有引诱作用的挥发性物质,研发基于行为调节的防治技术。[方法]采用Y型嗅觉仪测定了枣实蝇成虫对6种枣果挥发物的选择行为反应,并在室内试验的基础上进行田间引诱试验。[结果]室内试验结果显示,枣实蝇成虫对6种枣果挥发物具有显著正趋性。枣实蝇雌虫对棕榈酸乙酯、月桂酸乙酯、肉豆蔻酸的选择率较强,选择率均为30%以上;雄虫对亚油酸乙酯、油酸乙酯的选择率较强,选择率均为25%以上,从不同浓度梯度(原液、10%溶液、1%溶液)的选择率和选择系数来看,随浓度的递减,其选择率和选择系数也在递减,原液的选择率分别为10%溶液及1%溶液的2.5倍及5倍以上。田间试验发现:肉豆蔻酸对枣实蝇雌虫的诱集效果较强,2 d最多诱捕数量为15头·只^-1,平均诱捕数量为11.4头·只^-1,十四酸乙酯次之。在6种不同挥发物中油酸乙酯和亚油酸乙酯对枣实蝇雄虫的引诱效果较强。经单因素方差分析发现,在6种挥发性物质及混合物中,月桂酸乙酯及十四酸乙酯对枣实蝇雌雄虫的引诱效果之间存在显著性差异。其他挥发物之间无显著差异。[结论]枣果挥发物中棕榈酸乙酯、月桂酸乙酯、肉豆蔻酸与枣实蝇偏爱选择密切相关。在田间试验中:枣实蝇成虫对肉豆蔻酸的选择率较强,为研发枣实蝇引诱剂提供了理论依据。  相似文献   

New Polyketides from the Antarctic Fungus Pseudogymnoascus sp. HSX2#-11     

Ting Shi  Yan-Yan Yu  Jia-Jia Dai  Yi-Ting Zhang  Wen-Peng Hu  Li Zheng  Da-Yong Shi 《Marine drugs》2021,19(3)

The species Pseudogymnoascus is known as a psychrophilic pathogenic fungus with a ubiquitous distribution in Antarctica. Meanwhile, the study of its secondary metabolites is infrequent. Systematic research of the metabolites of the fungus Pseudogymnoascus sp. HSX2#-11, guided by the method of molecular networking, led to the isolation of one novel polyketide, pseudophenone A (1), along with six known analogs (2–7). The structure of the new compound was elucidated by extensive spectroscopic investigation and single-crystal X-ray diffraction. Pseudophenone A (1) is a dimer of diphenyl ketone and diphenyl ether, and there is only one analog of 1 to the best of our knowledge. Compounds 1 and 2 exhibited antibacterial activities against a panel of strains. This is the first time to use molecular networking to study the metabolic profiles of Antarctica fungi.  相似文献   

Identification of an H1N1 subtype of swine influenza virus and serological analysis     

Fa-chao SUN  Min TAN  Yuan-chao ZHANG  Yu-chao WANG  Sheng-liang CAO  Guo-fei DING  Fang-yuan CONG  Li-hong GUO  Si-dang LIU  Yi-hong XIAO 《农业科学学报》2019,18(7):1436-1442

To investigate the epizootic of swine influenza virus(SIV), 60 nasal swabs were collected from a clinical cases of pig farm in Tai'an City, Shandong Province of China in April 2017. SIV was isolated by inoculating into 10-day-old Special Pathogen Free embryonated eggs and the whole genome was sequenced. An H1N1 subtype SIV was isolated and designated as A/swine/Shandong/TA04/2017(H1N1). Phylogenetic analysis showed that apart from the polymerase A(PA) fragment belonging to the 2009 pandemic H1N1 branch, seven genome segments belonged to avian-like H1N1 influenza virus lineage. The cleavage site sequence of the hemagglutinin(HA) protein was PSIQSR↓G, which is a typical molecular biological characteristic. Five potential N-glycosylation sites(N14, N26, N277, N484 and N543) were found in the HA gene. To further investigate the epidemiology of SIV in this farm, the 995 serum samples were assessed with EAH1N1 2009 pandemic H1N1 and H3 N2 antigens. The results showed that the total positive rate was 65.43%. The positive rates of single virus infection detected by EAH1N1, 2009 pdmH1N1 and H3 N2 for serum HI(Hemagglutination inhibition) were 48.35, 30.85 and 7.47%, respectively. The results showed that SIV in Shandong Province has been reassorted in some segments and the SIV-positive rate was high on the SIV outbreak farm. These data provide evidence of an epizootic of SIV.  相似文献   

凉山半细毛羊新品种的选育技术与经验     

陈圣偶 《四川草原》2003,(3):29-32

凉山半细毛羊新品种的育成,是采用表型选择和基因型选择相结合产生的结果。在初生期、断奶期、育成期和成年期采取表型鉴定、逐步选择淘汰的制度。根据羔羊断奶期性状与育成期性状之间的显著相关性对羔羊单性状进行早期选择。应用同期发情与后裔测验相结合的方法,对种公羊基因型值用BLUP法进行选择。同时,加强育种工作的组织领导,组建独具特色的三级繁育体系,改善育种羊群的饲养管理条件,圆满完成了国家下达的半细毛羊新品种选育的任务,填补了国内粗档半细毛羊品种的空白。  相似文献   

以球果量、雄花量等数量性状对油松优良无性系的再选择   总被引：1，自引：0，他引：1  

刘梅  安守芹  吕馨  王永红 《内蒙古林业科技》2001,(Z1):6-9

本研究对油松无性系种子园 2 2个无性系的球果量和雄球花量进行观测调查 ,并对数据进行处理。通过无性系内和无性系间的方差分析、聚类分析和秩序排序法对各无性系进行分类和排序。研究结果表明 :无性系间球果量和雄球花量的差异极显著 ,而无性系内差异不显著。并筛选出 1 61 #,6#,2 9#,63 #,78#5个球果数量大 ,占总数的 46% ,雄花量高 ,占总数的 5 7.6%的无性系为优良无性系  相似文献   

菜用加工兼用栗永荆3号选育     

阮逸  李德静  叶茂宗 《浙江林业科技》2001,21(5):27-29

一般认为我国南方栗仅宜于菜用，加工极易开瓣和糊化，经过几年的筛选和加工试剂，永荆3号品种可生产B－E级标准的糖水栗子罐头。  相似文献   

北京植物园引种日本梅花品种初探   总被引：4，自引：3，他引：1  

黄亦工  孙宜 《北京林业大学学报》2004,(Z1)

北京植物园 1998年从日本引进梅花品种 39个。通过几年的观察、驯化 ,初步优选出可在北京地区生长的梅花品种 2 9个。 2 0 0 3年冬用电导法测定不同品种的抗寒力。对日本梅花品种在北京地区的栽培、养护及应用进行了初步探讨。  相似文献   

枣品种选择试验研究     

何佳林  李养志  李龙山  贺光生  高峰 《陕西林业科技》2001,(4):23-25,53

1997～1999年对收集引进的22个枣品种进行生长和结果情况调查及物候期定点预测，以大荔圆枣为对照，从丰产性、品质、抗性（抗裂果）、单果重、结果整齐度5个方面进行综合评比，筛选出适宜我省发展的不同用途的枣树优良品种12个，为黄土高原红枣产业化发展提供了品种依据。  相似文献