三江白猪线粒体DNA-RFLP的分析     

杨隽李馨  耿忠诚 《黑龙江畜牧兽医》2004,(3):11-13

采用22种限制性内切酶分析了三江白猪线粒体DNA的多态性。结果表明：在60头个体中检出30种限制性态型．归结为7种基因单倍型；其间的差异来自少数限制性位点的突变。单倍型间平均遗传距离为0．35%，群体遗传多态程度为0．044％。三江白猪遗传多样性非常贫乏，说明三江白猪与地方种猪起源于一个共同祖先，在品种形成早期受到创立者效应的制约。  相似文献   

The complete mitochondrial genome and molecular phylogeny of Lueyang black-bone chicken     

T. Zhang  H. Liu  L.-K. Yang  Y.-J. Yin  H.-Z. Lu  L. Wang 《British poultry science》2018,59(6):618-623

ABSTRACT

1. The objectives of the current study were to investigate the mitochondrial genome and molecular phylogeny of Lueyang black-bone chicken, and provide molecule base to preserve and explore the specific chicken strain.

2. Based on sequencing and clustering, the complete mitochondrial DNA map and sequences of Lueyang black-bone chicken were revealed, and two phylogenetic trees of Lueyang black-bone chickens based on D-loop sequences and the mitochondrial genome were constructed.

3. The results showed that the complete mitochondrial genome of Lueyang black-bone chickens is 16,784bp in size, consisting of 22 transfer RNA genes, two ribosomal RNA genes, 13 protein-coding genes, and one non-coding control region. The base composition of the complete mtDNA sequence is 30.28% for A, 23.78% for T, 32.42% for C, 13.52% for G. Additionally, 10 haplotypes of D-loop sequences in 32 Lueyang black-bone chickens were detected, which were distributed into 4 clades (A, B, C and E).

4. It was concluded that genetic diversity is wide in Lueyang black-bone chickens, and this strain has multiple maternal origins from different regions in China and neighbouring regions.  相似文献   

Phylogenetic analysis of Myanmar indigenous chickens using mitochondrial D-loop sequence reveals their characteristics as a genetic resource     

Su Lai Yee Mon  Moe Lwin  Aye Aye Maw  Lat Lat Htun  Saw Bawm  Kotaro Kawabe  Yasuhiko Wada  Shin Okamoto  Takeshi Shimogiri 《Animal Science Journal》2021,92(1):e13647

Myanmar indigenous chickens play important roles in food, entertainment, and farm business for the people of Myanmar. In this study, complete mitochondrial D-loop sequences (1232 bp) were analyzed using 176 chickens, including three indigenous breeds, two fighting cock populations, and three indigenous populations to elucidate genetic diversity and accomplish a phylogenetic analysis of Myanmar indigenous chickens. The average haplotype and nucleotide diversities were 0.948 ± 0.009 and 0.00814 ± 0.00024, respectively, exhibiting high genetic diversity of Myanmar indigenous chickens. Sixty-four haplotypes were classified as seven haplogroups, with the majority being haplogroup F. The breeds and populations except Inbinwa had multiple maternal haplogroups, suggesting that they experienced no recent purifying selection and bottleneck events. All breeds and populations examined shared haplogroup F. When 232 sequences belonging to haplogroup F (79 from Myanmar and 153 deposited sequences from other Asian countries/region) were analyzed together, the highest genetic diversity was observed in Myanmar indigenous chickens. Furthermore, Myanmar indigenous chickens and red junglefowls were observed in the center of the star-like median-joining network of 37 F-haplotypes, suggesting that Myanmar is one of the origins of haplogroup F. These findings revealed the unique genetic characteristic of Myanmar indigenous chickens as important genetic resources.  相似文献   

DNA barcoding for identification of cryptic species in the field and existing museum collections: a case study of Aethomys and Micaelamys (Rodentia: Muridae)     

Metlholo Andries Phukuntsi  Helene Brettschneider  Desiré L Dalton  Teresa Kearney  Jacqueline Badenhorst  Antoinette Kotze 《African Zoology》2016,51(1):69-76

DNA barcoding has been proposed as a method for species identification. However, this method has been criticised for its over-reliance on a single mitochondrial gene. In this study, four mitochondrial gene regions and one nuclear gene region were used to investigate their different abilities to identify tissue associated with museum specimens of Aethomys chrysophilus, Aethomys ineptus and Micaelamys namaquensis. Aethomys chrysophilus and the more recently elevated A. ineptus are indistinguishable on morphological grounds; however, their ranges are largely parapatric with only one syntopic locality currently known. All of the mitochondrial gene regions were able to separate M. namaquensis from A. chrysophilus and A. ineptus, but they varied in their abilities to resolve differences between A. chrysophilus and A. ineptus. The sequence results identified a specimen from KwaZulu-Natal that was misclassified and should have been identified as A. ineptus. Seven specimens that had not been reclassified following the elevation of A. ineptus to species level were identified as A. ineptus. Individuals of A. chrysophilus from Malawi could not be classified as either A. chrysophilus or A. ineptus, and may be a hybrid or a new, distinct species. This study indicates that DNA barcoding may be used to separate M. namaquensis from A. chrysophilus and A. ineptus, and although it was not able to separate A. chrysophilus and A. ineptus, it did indicate specimens from Malawi may be a new cryptic species.  相似文献   

对两个玉米雄性不育系的细胞质分类研究   总被引：3，自引：0，他引：3  

李建生  徐尚忠 《作物学报》1993,19(2):156-164

本文从普通遗传学、植物病理学和分子生物学的水平对玉米雄花不育系—元徐cms—小黄和恩二激cms—大黄进行了综合性的分类研究。大田的育性反应表明，这两个不育系的育性恢复受一对显性基因控制。对花粉染色的观察发现F1可育花粉与败育花粉基本上呈1：1分离。用小斑病T小种接种，这两个不育系不表现专化感染。通过mtDNA的分析  相似文献   

Attaining inter-subgeneric hybrids in fragrant azalea breeding and the inheritance of organelle DNA   总被引：1，自引：0，他引：1  

Nobuo Kobayashi  Daiki Mizuta  Akira Nakatsuka  Masaru Akabane 《Euphytica》2008,159(1-2):67-72

Inter-subgeneric hybrids were successfully obtained in reciprocal cross combinations between evergreen azaleas (Rhododendron nakaharae and its hybrids) and fragrant deciduous azaleas (R. arborescens and R. viscosum) for the purpose of fragrant evergreen azalea breeding. Nuclear and organelle DNA of these hybrids was investigated using PCR-RFLP markers. Viable hybrid seedlings have nuclear ribosomal DNA (nrDNA) inherited biparentally, mitochondrial DNA (mtDNA) from the seed parent, and chloroplast DNA (cpDNA) from the deciduous azalea, regardless of cross combination. These results suggest that the chloroplast genome from deciduous azaleas and the nuclear genome from evergreen azaleas are compatible in viable hybrid progenies.  相似文献   

塔里木兔基因组DNA提取及PCR扩增检测     

蒋萍  李旭辉  王丹  单文娟 《新疆农垦科技》2009,32(3):39-41

本研究通过用CTAB法和蛋白酶K法提取塔里木兔基因组DNA,再利用琼脂糖凝胶电泳和聚合酶链反应（PCR）扩增线粒体DNA控制区（D-loop）因来进行检测,为塔里木兔的遗传学研究提供基本实验素材。  相似文献   

Using FTA® Elute MicroCards to address biosecurity and DNA quality issues in abalone aquaculture     

Natasha A Carr  Sharon A Appleyard 《Aquaculture Research》2008,39(16):1799-1802

  相似文献   

线粒体DNA在动物分子系统学研究中的应用   总被引：1，自引：0，他引：1  

戴伟  王晓梅  杨华 《天津农学院学报》2005,12(2):48-53

mtDNA由于其自身的特点和优越性,已被广泛地应用于动植物分子系统学的研究。本文概述了mtDNA的基本特征、遗传特点和用于系统分类的优点,并对mtDNA在动物分子系统学中的部分研究成果做了概括。  相似文献   

烟粉虱复合种不同地理种群的遗传分化   总被引：13，自引：0，他引：13  

褚栋  刘国霞  范仲学  陶云荔  张友军 《中国农业科学》2006,39(8):1571-1580

烟粉虱Bemisia tabaci(Gennadius) 是由具有遗传分化的不同地理种群组成的复合种。本文介绍了烟粉虱不同地理种群遗传分化的最新研究进展，并在世界各国对烟粉虱核糖体ITS1(rDNA ITS1)、线粒体COI（mtDNA COI）基因大量测序的基础上，进一步分析了烟粉虱不同地理种群的遗传分化。根据mtDNA COI和rDNA ITS1基因序列分析的结果，烟粉虱不同地理种群可分为5组，即亚洲组（Asia group）、美洲组（America group）、非洲组（Africa group）、澳洲组(Australia group)、B型/地中海/中东/北非/Ms型组(Biotype B/Mediterranean/Middle Eastern/Northern Africa/Biotype Ms group)；此外，还包括3个没有特定组的种群，即乌干达（Uganda）、科特迪瓦（Ivory Coast和台湾（Taiwan）种群。地理隔离可能是造成烟粉虱不同地理种群遗传分化的最重要驱动力。许多具有入侵性或生物学优势的烟粉虱种群随着人类活动而传入新的地区，造成了严重的经济损失。有必要加强烟粉虱生物型的监测，遏制已入侵烟粉虱种群的蔓延，防止新的具有潜在危险性的烟粉虱种群传入中国。  相似文献