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AIM: To observe the antiproliferative effect of c-myc antisense oligonucleotide in rat thymus lymphocytes. METHODS: Rat thymus lymphocytes were separated by Ficoll-Urografin density gradient centrifugation. Lipofectin was used to introduce antisense, sense and mismatched oligonucleotides for c-myc to rat thymus lymphocytes. The antiproliferative effect was assayed by incorporation of [3H]-TdR and MTS cell proliferation assay. TR-PCR was used to detect the expression of c-myc mRNA. RESULTS: c-myc antisense oligonucleotide inhibited ratthymus lymphocytes proliferation[(0.14±0.03)A vs(0.32±0.16)A,P<0.05],but this ef ect had no relationship with the concentration of c-myc antisense oligonucleot ide.c-myc antisense oligonucleotide decreased the expression of c-myc mRNA in rat thymus lymphocytes. CONCLUSION: c-myc antisense oligonucleotide inhibited rat thymus lymphocyte proliferation. 相似文献
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目的 :探讨p5 3、bcl 2和c myc蛋白在甲状腺肿瘤中的表达意义。 方法 :应用免疫组化EnvisionTM法对 73例甲状腺肿瘤中 p5 3、bcl 2和c myc蛋白的表达进行检测。 结果 :3 6例腺瘤中 p5 3、bcl 2和c myc蛋白的阳性表达率分别为 :0 0 %( 0 / 3 6)、88.6%( 3 2 / 3 6)和 5 0 .0 %( 18/ 3 6) ;3 7例腺癌则分别为 :2 1.6%( 8/ 3 7)、64 .9%( 2 4/ 3 7)和 89.2 %( 3 3 / 3 7) ;p5 3、bcl 2、c myc蛋白分别在 3 6例腺瘤与 3 7例腺癌之间比较差异均有显著性 (P <0 .0 5或P <0 .0 1)。p5 3蛋白阳性表达的腺癌 8例 ,其bc1 2及c myc蛋白阳性表达率分别为 75 .0 %( 6/ 8)和 10 0 %( 8/ 8) ,在腺癌中 p5 3蛋白表达与bcl 2蛋白表达仅呈低度正相关 (r =0 .3 5 1,P <0 .0 5 )。 5例淋巴结转移性甲状腺癌中p5 3蛋白阳性表达率为 60 0 %( 3 / 5 ) ,但均无bc1 2蛋白阳性表达。结论 :p5 3、bcl 2及c myc蛋白可能共同参与甲状腺癌的发生发展 ;检测 p5 3蛋白有助于甲状腺良恶性肿瘤的鉴别 相似文献
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AIM: To observe the inhibitory effect of antisense eukaryotic expression vectors for c-myc on rat airway smooth muscle cells. METHODS: Antisense and sense eukaryotic expression vectors for c-myc pcDNA3-myc-antisense and pcDNA3-myc-sense were constructed. Lipofectin was used to introduce antisense and sense eukaryotic expression vectors for c-myc into rat. The inhibitory effect was assayed by MTT cell proliferation assay. Cell cycles were detected by flow cytometry technology. The expression of c-Myc was detected by immunohistochemistry. RESULTS: The results showed that antisense eukaryotic expression vector for c-myc inhibited rat airway smooth muscle cells proliferation. Rat airway smooth muscle cells were prohibited in S phase and the expression of c-Myc was decreased after antisense eukaryotic expression vectors for c-myc were transfected into cells. CONCLUSION: Antisense eukaryotic expression vectors for c-myc inhibit rat airway smooth muscle cell proliferation. 相似文献
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Providing an appropriate negative control for the experimental factor arbuscular mycorrhiza (AM) is a fundamental methodological problem. Therefore, the nonmycorrhizal (myc –) and nonnodulating (nod –) pea (Pisum sativum L.) mutant P2 was studied together with the parental symbiotic isogenetic variety Frisson in three experiments: (1) growth response to water supply in a climate chamber under nonsymbiotic growth conditions, (2) field evaluation at three sites in the Alentejo, South Portugal, and (3) growth response to P supply in a soil low in available P in a greenhouse‐chamber experiment. In the climate chamber at high NPK levels, mutant P2 achieved the same biomass as Frisson at 80% and 40% water‐holding capacity, respectively. For the field evaluation, three sites were chosen with normal arable use (Évora), extensive use as Montado (Portel), and intensive horticultural use (Mitra). The colonization of pea roots with AM fungi ranged from 4% (Mitra) to more than 90% (Portel), probably caused by differences in P availability. The plant density of mutant P2 was generally 25% lower than that of Frisson . Yield indices were all lowest at Portel, despite the same NPK fertilization. Grain and shoot yield of mutant P2 did not reach the level of Frisson at any site. Differences in N and P concentrations between the two isolines were insignificant in most cases. Differences in the amount of shoot P per plant consistently mirrored the mycorrhizal status of the three sites. Roughly 50% of the yield depression per m2 could be attributed to the lower plant density of mutant P2, the remaining 50% must be caused by AM‐fungal colonization or other factors. In the final pot experiment using the soil with low P availability from Portel, the main benefit of AM for peas was enhanced P uptake. Central questions could not be answered using a nonmycorrhizal control. However, mutants remain one interesting tool, best be used in combination with other approaches to estimate the effects of mycorrhization. 相似文献
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Archival formalin-fixed tissues from wild-caught adult blue sharks, Prionace glauca (L.), were used for immunocytochemical detection of proliferating cell nuclear antigen (PCNA), two oncoproteins from the oncogenes c-myc and pan-ras, and a protein product from the tumour suppressor gene p-53. All sharks were caught during summer months between 2000 and 2006 by recreational fishermen off the USA coast in the northwestern Atlantic. The sharks were necropsied on landing and selected organ samples were collected into elasmobranch formalin and processed for paraffin embedding and light microscopy. Paraffin-embedded sections from collected tissue were both stained with haematoxylin and eosin and processed by immunocytochemical techniques using antibodies raised against the PCNA, p-ras, c-myc and p-53 proteins. The lesions examined in this study included two well differentiated adenomatous gastric polyps, a testicular capsular mesothelioma, a gingival fibropapilloma with elements of ameloblastoma, three liver tumours, two pericardial fibropapillomas and six cases of proliferative serositis (pericarditis and peritonitis). Normal and hyperplastic tissues from blue sharks, and human neoplastic tissues served as negative and positive controls, respectively. We detected upregulation of PCNA in many neoplastic, one dysplastic and in some hyperplastic lesions, and positive p-ras and c-myc signals in some of the neoplastic lesions. None of the examined tissues showed positive p-53 signalling. This is the first literature report on immunocytochemical detection of molecular markers of cancer in sharks and in fish of the class Chondrichthyes. 相似文献
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