Pheromone-based communication disruption ofAdoxophyes orana on peach using the new RAK 3+4 dispensers and their effect on development of fruit rot diseases     

E. Navrozidis  T. Thomidis  T. Sipouridis  I. Xatzicharisis  I. Fotiadis  D. Servis 《Phytoparasitica》2005,33(2):149-153

The effectiveness of the pheromone-based communication disruption method was examined against the summerfruit tortrix,Adoxophyes orana F.v.R. (Lepidoptera: Tortricidae), a pest of peach trees, using the new RAK 3+4 dispenser (BASF). NoA. orana males were captured in pheromone traps inside the experimental orchards, which were saturated with the RAK 3+4 dispensers. The percent of damaged leaves was practically zero, while the level of damaged fruits was 0–6% in pheromone-treated orchards. The percentage of fruit rot caused byMonilinia laxa was lower in pheromone-based communication disruption orchards than in the control. It was concluded that the RAK 3+4 dispenser could be used againstA. orana as an economical and environmentally friendly method.  相似文献   

Variation in virulence of Greek isolates ofPhytophthora citrophthora as measured by their ability to cause crown rot on three peach rootstocks     

Thomas Thomidis 《Phytoparasitica》2002,30(2):191-193

The virulence ofPhytophthora citrophthora isolated from various host-plants on three peach rootstocks (GF677, PR204, KID I) was examined. There was no significant difference among the rootstocks with respect to their susceptibility to testedP. citrophthora isolates. The most virulent isolate originated from sycamore (Acer pseudoplatanus); isolates from pistachio trees (Pistacia vera) also showed high virulence but were significantly less virulent than the sycamore isolate. Isolates originating from plum (Prunus domestica), almond (Prunus amygdalus) and lemon (Citrus limon) trees were moderately virulent on peach rootstocks; those from cyclamen (Cyclamen persicum) showed the lowest virulence of those tested. There was thus great variation in virulence among the testedP. citrophthora isolates. It is possible that the isolates ofP. citrophthora from sycamore, pistachio, plum, almond and lemon trees are a threat to peach trees, whereas the low virulence of the isolates from cyclamen hosts suggests that these pathogens are not a serious threat to peach trees. http://www.phytoparasitica.org posting Jan. 3, 2002.  相似文献   

鲁西南地区果树需冷量的研究   总被引：2，自引：0，他引：2  

宋颖  史作安  高东升 《落叶果树》2002,34(6):1-3

2000～2001年,采用Utah加权模型,先后对鲁西南地区常见的葡萄、桃和油桃、杏品种的需冷量进行了研究。结果表明:不同树种、品种的需冷量差异显著。葡萄的需冷量最高,为1010～1840c.u。桃和油桃最低,花芽为620～910c.u,叶芽为500～890c.u。杏介于二者之间,花芽和叶芽均为790～910c.u。各树种、品种的需冷量年际间均有差异。葡葡、杏不同品种需冷量的高低与本品种果实成熟期早晚的关系表现为成熟期早,需冷量低;成熟期晚,需冷量高。但桃树不同品种的需冷量高低与本品种的果实成熟期无明显相关关系,果实成熟期早,但需冷量较高的情况普遍存在。同一品种其花芽、叶芽的需冷量不一,基本趋势是花芽的需冷量高于叶芽。  相似文献   

肥城桃组培苗诱导、基因转化及其增殖   总被引：11，自引：0，他引：11  

李曜东  魏玉凝 《果树学报》2003,20(1):70-72

红里、白里是肥城桃的两个优良品种,但不耐贮藏。为试图利用基因工程解决这一问题,先通过组培将其胚、胚乳、子叶的愈伤组织分别诱导再生植株和芽剥离出茎尖培育成苗。再将肥城桃反义PG基因,通过农杆菌介导转化组培苗,转化苗在含卡那霉素培养基上筛选,获得抗卡那霉素的转基因苗。用研制的增殖培养基培养4～7周,一个苗可生长出14～18分枝的苗,解决了转基因苗获得率低的问题,也满足了对转基因株系进行检测和从组培室到温棚到田间果园过渡栽培和芽接的需求,提高了繁育速率。  相似文献   

Identification and expression analysis of 11 MADS-box genes in peach (Prunus persica var. nectarina ‘Luxing’)     

Hui-Feng Li  Qing-Long Dong  Gui-Xiang Li 《The Journal of Horticultural Science and Biotechnology》2018,93(3):232-243

MADS-box genes play a central role in the development of flowers in plants. In this study, 11 PpMADSs were isolated from ‘Luxing’ peach, and the expression levels were detected in different tissues and fruit development. Eleven PpMADSs, designated as PpMADS13, 14, 18, 23, 24, 25, 32, 33, 34, 35, and 39 were isolated. Phylogenetic analysis revealed that PpMADS13, 14, and 18 belonged to SVP, AGL15, and MIKC* group respectively; PpMADS23, 24, 25, and 39 were in the Mα group; PpMADS32, 33, 34, and 35 belonged to the Mγ group. Predictions from subcellular localization showed that 10 PpMADS were located in the nucleus. RT-PCR revealed that PpMADS13 was expressed in stems, leaves, and during fruit development (70d); PpMADS14 was expressed in sepals, stamens, petals, and during flower development; PpMADS18 was expressed in roots, stems, leaves, sepals, ovaries, stamens, petals, and during flower and fruit development; all MADS-box genes (expect for PpMADS33) in the Mα and Mγ group were expressed in roots, stems, leaves, sepals, ovaries, stamens, petals, and during flower development; few genes were expressed during fruit development. These results indicated that PpMADS may play a crucial regulatory role in vegetative growth and development processes of flowers and fruits in peaches.  相似文献   

不同品种桃果冷链物流特性差异性研究   总被引：1，自引：0，他引：1  

周慧娟  叶正文  苏明申  张安宁  杜纪红  刘伟 《经济林研究》2016,(1):99-106

为了探讨不同品种桃果冷链物流特性的差异化问题,从而为其贮运技术的优化提供参考依据,分别以采自山东省蒙阴县岱崮镇的‘仓方早生’、‘春美’、‘中油4号’和‘锦香’黄桃果实为试材,对不同品种桃果实在长途(山东蒙阴至新加坡)冷链物流期间的预冷特性、蓄冷特性、货架期及其品质的变化情况进行了观察,并对其果心温度在冷链物流期间的变化情况进行了全程跟踪检测。结果表明:在整个冷链物流期间,‘中油4号’和‘春美’的果心温度一直低于其余2个品种,说明其均有较好的预冷和蓄冷性能;在山东冷库温度设为0℃、预冷40 h的条件下,不同品种桃果果心温度方能降低至2.5~3.0℃,说明产区桃果贮运企业的冷库制冷能力还有待提高,或需配置专门的预冷设备和优化预冷技术;货架期第2天(到达目的地后),4个品种桃果果肉组织的硬度均在2 kg·cm-2以上;其中‘仓方早生’和‘锦香’果实中可溶性固形物的含量略高于10%,其余品种的均为8%~9%,说明产地采收果实的品质有待提高;不同品种对冷害的敏感性存在一定的差异性。文中综合分析认为:‘中油4号’的贮运品质较好,其预冷和蓄冷性能均佳,其果实外观和肉质均好,且汁液多,味较甜;而其他品种桃果运至目的地后均发生了不同程度的冷害现象。据此,文末提出了采取终端冷链措施以避免冷害发生的建议。  相似文献   

122份栽培桃品种（系）黄白肉性状的分子标记辅助鉴定     

鲁振华  沈志军  牛良  潘磊  崔国朝  曾文芳  王志强 《中国农业科学》2020,53(14):2929-2940

【目的】 类胡萝卜素裂解双脱氧酶基因（Carotenoid Cleavage Dioxygenases 4,CCD4）控制桃果肉颜色（白/黄）,CCD4存在3种等位基因。本研究利用Indel、SSR荧光标记毛细管电泳及SNP鉴定等基因分型技术分析我国主要桃黄白肉品种（系）中CCD4等位基因的差异,为主要黄/白肉品种（系）的基因型鉴定、亲本选配和选择相应的分子标记对不同来源子代的果肉颜色进行鉴定奠定基础。【方法】利用已经报道的桃不同果肉颜色中CCD4等位基因3种突变类型,合成不同引物进行PCR扩增,LTR反转录转座子插入突变经1%的琼脂糖凝胶电泳检测,CT单元重复的PCR产物在ABI3730XL测序仪上进行SSR荧光标记毛细管电泳检测,SNP标记经Sanger测序后利用ContigExpress软件分析CCD4等位基因的碱基替换（A→T）。综合以上结果,统计每份材料中CCD4等位基因的突变类型与果肉颜色的一致性。【结果】通过对不同来源的122份桃品种（系）材料进行基因型分析,发现CCD4发生LTR反转录转座子插入突变材料的基因型共有31份,占总材料的25.4%,其中纯合插入突变材料的片段扩增长度为729 bp,共有8份,占总突变的25.8%;CCD4发生微卫星重复序列突变材料存在2 bp的插入,扩增片段长度为179 bp,该类型共有68份,占总材料的55.7%,其中纯合插入材料25份,占总突变的36.8%;CCD4发生A→T碱基替换突变的材料较少,仅有1份,占总材料的0.82%,实际应用中可以不考虑该种类型。CT和LTR插入的两种突变类型的黄肉品种（系）有7份,占总材料的5.7%。研究结果表明,LTR反转录转座子插入突变和微卫星序列重复突变是黄肉桃中CCD4等位基因的主要突变类型。其中CCD4发生一种纯合突变或两种杂合突变桃品种（系）为黄肉类型,分子标记鉴定结果与调查的122份桃品种（系）黄白肉表型性状完全一致,准确率为100%。【结论】采用分子标记明确了122个桃品种（系）黄/白肉性状的基因型,为不同亲本组合子代表型鉴定的标记类型选择提供了技术支撑,为建立桃种质材料黄/白性状的分子辅助育种体系和黄肉桃的选育奠定了基础。  相似文献   

哈密设施桃红蜘蛛的危害与防治     

曹丽艳  胡学林  王建春  何海娟 《现代园艺》2015,(6)

新疆哈密地区特有的环境条件为红蜘蛛的发生创造了强有力的生态环境,经多年的栽种经验,归纳总结了红蜘蛛的发生规律、造成的危害以及综合防治方法,有效的防治红蜘蛛的发生。  相似文献   

桃PpSnRK1α在番茄中过表达对营养胁迫下植株生长的影响     

罗静静  张亚飞  彭妍  彭福田  赵永飞  于雯 《园艺学报》2017,44(4):644-652

以番茄(Solanum lycopericum)超表达桃SnRK1(蔗糖非发酵蛋白激酶–1)基因PpSnRK1α的株系及野生型为试材,研究在养分供应不足时SnRK1对植株生长的影响。结果表明,低营养条件下,转基因番茄叶片和根系中的Sn RK1酶活性比野生型高41.55%和39.46%;功能叶片的净光合速率平均比野生型高18.98%;低营养胁迫12 d的叶片SOD、POD、CAT活性比野生型高35.56%、28.85%和14.90%;根系活力比野生型高26.39%;茎和叶中氮磷含量显著高于野生型,钾含量两者差别不大,在根系中氮磷含量差别不大,而钾含量显著高于野生型,且氮素向地上部茎和叶中的分配比率增加。上述结果说明,在营养缺乏条件下,超表达PpSnRK1α可以提高番茄功能叶净光合速率,促进植株对氮素的吸收利用,从而延缓叶片衰老。  相似文献   

4份桃种质挥发性成分的GC-MS分析   总被引：1，自引：0，他引：1  

罗静  黄玉南  王超  乔成奎  庞涛  方金豹  谢汉忠 《经济林研究》2016,(3)

为了分析桃果实中共有的挥发性成分及初步探讨果皮毛和果肉颜色性状对挥发性成分的影响情况,以此2对性状均存在差异的4份桃种质为试材,采用顶空固相微萃取方法提取其成熟果肉中的挥发性成分,并用气相-质谱联用仪对其进行了测定。结果表明:4份桃种质中共鉴定出116种挥发性成分,其主要为酯、醛、醇和烷烃等类物质。从挥发性成分的种类数量上看,白肉桃‘大久保’和‘华光’中检出的挥发性成分种类较多,分别有68和52种;而2份黄肉桃种质中检出的挥发性成分种类均较少。从挥发性成分的种类上看,2份普通桃与2份油桃中的差异挥发性成分总共仅有3种,其分别为反式-2,4-庚二烯醛、戊酸-2,2,4-三甲基-3-异丙酸-异丁酯和脱氧精胍菌素,远低于2份白肉桃与2份黄肉桃种质中差异挥发性成分的数量(13种)。此外,从挥发性成分的类群上看,2份白肉桃含有较多的"青香型"的C6类化合物,而2份黄肉桃含有较多的"果香型"的酯和内酯类化合物。文中综合分析认为,果皮毛的有无和果肉的颜色性状均可能影响果实中挥发性成分的数量和种类,但后者的影响较为明显。  相似文献